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The map label for this gene is lacZ [H]

Identifier: 29350075

GI number: 29350075

Start: 6116237

End: 6118684

Strand: Reverse

Name: lacZ [H]

Synonym: BT_4667

Alternate gene names: 29350075

Gene position: 6118684-6116237 (Counterclockwise)

Preceding gene: 29350076

Following gene: 161511186

Centisome position: 97.74

GC content: 50.2

Gene sequence:

>2448_bases
ATGAACATGAAACACCAGAAACAGTTGTTTACTGTACTTTTGATGAGCGCAGCCTGTATGCTACAGGCACAGCGCAGCGA
GACGCTGCTTGAGAAGAACTGGAAATTCAGTAAAGGAGATTTTCCGGAAGCTGCGCAGACTGACTACAATGATACTAAAT
GGGAATCGGTCGTTATCCCGCATGACTGGGCCATCTTCGGACCTTTCGACATGAACAACGACCTGCAAAACGTAGCAGTC
ACACAGAACTTTGAGAAGAAGGCATCACTGAAAACCGGACGTACCGGAGGACTGCCTTATGTCGGAACCGGATGGTATCG
CACCAGCTTCGACGTTCCCGCCGACAAAGAAGTCACTCTCCTGTTCGACGGAGCAATGAGTGAGGCGCGTGTATACGTCA
ACGGACAGGAGGCTTGCTTCTGGCCCTTCGGCTACAACTCTTTCCATTGCAACGTCACCCCTTTCCTGAATAAAGACGGA
AAGAACAATACACTGGCGGTACGCCTCGAAAACCGCCCCCAGTCTTCACGCTGGTATCCGGGAGCAGGTCTGTACCGCAA
CGTCCACTTGATCGTGACGGAAAAAGTACACATTCCGGTATGGGGCACGCAGATTACTACCCCGCATGTCAGTGATGAAT
TTGCCGCCGTACGCCTGCAAACCAAGATAGAGAATGCCGGCGAAAAGACAGAAATCCGTATAGAGACAGAAATCCTGTCA
CCGGATGGAAAGGTAGTGACCCGCAAAGAAAACAAAGGCCGCATCAATCACGGGCAGCCGTTTGAACAAAACTTCATCGT
CAACGCGCCTCAACTCTGGTCACCGGAAAGCCCCGCTCTTTACAAGGCTGTATCGAAAGTGTACGCAGACAACCAACTGG
TAGATACATACTCTACCCGCTTCGGTATCCGCAGCATTGAATACATTGCCGACAAAGGTTTCTACTTGAACGGAAAACAC
CGCAAATTCCAAGGTGTATGCAACCACCATGACTTAGGTCCGCTGGGTGCGGCCATCAACGTAGCGGCACTTCGCCACCA
GCTCACACTCTTAAAGGATATGGGATGCGACGCCATTCGTACTTCCCATAATATGCCGGCTCCCGAACTCGTGGAATTGT
GCGACGAAATGGGATTCATGATGATGATCGAACCGTTCGACGAATGGGACATCGCCAAGTGTGAAAACGGATATCACCGT
TACTTCAACGAATGGGCAGAACGCGATATGGTGAATATGCTGCACAACTATCGTAACAATCCCTGTGTAGTGATGTGGAG
TATCGGTAATGAAGTCCCCACGCAATGCAGCCCTGTAGGATATAAAGTAGCCAAGTTCCTGCAAGATATCTGCCACCGTG
AAGACCCTACCCGCCCCGTTACCTGCGGCATGGACCAAGTGACTTGTGTTCTAGCCAACGGATTTGCTGCCATGATCGAT
ATCCCCGGTCTGAACTACCGCGCCAATCGCTATCAGGAGGCATATGACAAACTTCCGCAAAATCTTATTCTGGGTAGTGA
AACTGCGTCCACCGTCAGCTCACGCGGTGTCTACAAGTTTCCCGTAAAAGATAAGAAAAGTGCCCAGTACGATGATCATC
AATGTTCTTCTTATGACGTAGAGGCATGTTCATGGTCCAACATCCCCGATGAGGACTTCGCTTTGGCGGACGATCATCAC
TGGACCATCGGACAGTTTGTATGGACCGGCTTCGATTATCTGGGTGAGCCTTCCCCTTATGATACTGATGCCTGGCCCAA
TCATAGTTCCATGTTCGGTATTATCGACCTTGCCAGTCTCCCCAAAGACCGCTATTACTTGTATCGCAGCATATGGAATA
AGGAAGCGGAAACACTGCATATCCTGCCGCATTGGACATGGCCGGGACGCGAAGGTGAAGTAACTCCGGTATTTGTTTAC
ACCAACTACCCTGCTGCCGAACTGTTTATCAACGGAAAAAGCTACGGTAAACAAAGCAAGAACAACAGTTCACTGAAAAG
CCGCTACCGTCTGATGTGGATGGATGCCATATATGAACCGGGTGAAGTGAAAGTAGTAGCCTACGATAAAGACGGAAAAG
CAGTAGCAGAAAAGAGTGTACGCACCGCAAGCAAGCCTCATCACATCGAACTGGTCAGCAACCGGAACGAACTGACGGCA
GACGGCAAAGACCTCGCTTATGTGACAGTGAAAGTGGTGGACAAAGACGGCAACCTCTGCCCTGCCGACAGTCGTCTGAT
CAACTTCTCCGTCAAAGGTGCCGGAAAATACCGTGCCGGAGCCAACGGTGACCCCACGAGTCTCGACCTGTTCCATCTTC
CGAAGATGCACGCTTTCAACGGAATGCTTACAGCTATCGTACAAACAAATGAAACAGCCGGAGAAATTATTCTGACTGCC
AAAGCCGGCGGAGTGAAAGCAGGAAGTATCCGTCTTCTGACGAAATAA

Upstream 100 bases:

>100_bases
GCCCCGAATGGCTATAATGACGGTTATAACCTAGGCTGTTTCGATAATAATGCGCCTACAATTGCACTAGATGCTTTTAA
AATCCAATAAAAAAGTGAGA

Downstream 100 bases:

>100_bases
TCGGTCTACATAAAGAAATATATTTGAAAAACTGCTGTCACCTGTCACCAAACGAGTGATAATAGTCTGATAATTAAATC
ATAAGACCGTGACAGCAACG

Product: beta-galactosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 815; Mature: 815

Protein sequence:

>815_residues
MNMKHQKQLFTVLLMSAACMLQAQRSETLLEKNWKFSKGDFPEAAQTDYNDTKWESVVIPHDWAIFGPFDMNNDLQNVAV
TQNFEKKASLKTGRTGGLPYVGTGWYRTSFDVPADKEVTLLFDGAMSEARVYVNGQEACFWPFGYNSFHCNVTPFLNKDG
KNNTLAVRLENRPQSSRWYPGAGLYRNVHLIVTEKVHIPVWGTQITTPHVSDEFAAVRLQTKIENAGEKTEIRIETEILS
PDGKVVTRKENKGRINHGQPFEQNFIVNAPQLWSPESPALYKAVSKVYADNQLVDTYSTRFGIRSIEYIADKGFYLNGKH
RKFQGVCNHHDLGPLGAAINVAALRHQLTLLKDMGCDAIRTSHNMPAPELVELCDEMGFMMMIEPFDEWDIAKCENGYHR
YFNEWAERDMVNMLHNYRNNPCVVMWSIGNEVPTQCSPVGYKVAKFLQDICHREDPTRPVTCGMDQVTCVLANGFAAMID
IPGLNYRANRYQEAYDKLPQNLILGSETASTVSSRGVYKFPVKDKKSAQYDDHQCSSYDVEACSWSNIPDEDFALADDHH
WTIGQFVWTGFDYLGEPSPYDTDAWPNHSSMFGIIDLASLPKDRYYLYRSIWNKEAETLHILPHWTWPGREGEVTPVFVY
TNYPAAELFINGKSYGKQSKNNSSLKSRYRLMWMDAIYEPGEVKVVAYDKDGKAVAEKSVRTASKPHHIELVSNRNELTA
DGKDLAYVTVKVVDKDGNLCPADSRLINFSVKGAGKYRAGANGDPTSLDLFHLPKMHAFNGMLTAIVQTNETAGEIILTA
KAGGVKAGSIRLLTK

Sequences:

>Translated_815_residues
MNMKHQKQLFTVLLMSAACMLQAQRSETLLEKNWKFSKGDFPEAAQTDYNDTKWESVVIPHDWAIFGPFDMNNDLQNVAV
TQNFEKKASLKTGRTGGLPYVGTGWYRTSFDVPADKEVTLLFDGAMSEARVYVNGQEACFWPFGYNSFHCNVTPFLNKDG
KNNTLAVRLENRPQSSRWYPGAGLYRNVHLIVTEKVHIPVWGTQITTPHVSDEFAAVRLQTKIENAGEKTEIRIETEILS
PDGKVVTRKENKGRINHGQPFEQNFIVNAPQLWSPESPALYKAVSKVYADNQLVDTYSTRFGIRSIEYIADKGFYLNGKH
RKFQGVCNHHDLGPLGAAINVAALRHQLTLLKDMGCDAIRTSHNMPAPELVELCDEMGFMMMIEPFDEWDIAKCENGYHR
YFNEWAERDMVNMLHNYRNNPCVVMWSIGNEVPTQCSPVGYKVAKFLQDICHREDPTRPVTCGMDQVTCVLANGFAAMID
IPGLNYRANRYQEAYDKLPQNLILGSETASTVSSRGVYKFPVKDKKSAQYDDHQCSSYDVEACSWSNIPDEDFALADDHH
WTIGQFVWTGFDYLGEPSPYDTDAWPNHSSMFGIIDLASLPKDRYYLYRSIWNKEAETLHILPHWTWPGREGEVTPVFVY
TNYPAAELFINGKSYGKQSKNNSSLKSRYRLMWMDAIYEPGEVKVVAYDKDGKAVAEKSVRTASKPHHIELVSNRNELTA
DGKDLAYVTVKVVDKDGNLCPADSRLINFSVKGAGKYRAGANGDPTSLDLFHLPKMHAFNGMLTAIVQTNETAGEIILTA
KAGGVKAGSIRLLTK
>Mature_815_residues
MNMKHQKQLFTVLLMSAACMLQAQRSETLLEKNWKFSKGDFPEAAQTDYNDTKWESVVIPHDWAIFGPFDMNNDLQNVAV
TQNFEKKASLKTGRTGGLPYVGTGWYRTSFDVPADKEVTLLFDGAMSEARVYVNGQEACFWPFGYNSFHCNVTPFLNKDG
KNNTLAVRLENRPQSSRWYPGAGLYRNVHLIVTEKVHIPVWGTQITTPHVSDEFAAVRLQTKIENAGEKTEIRIETEILS
PDGKVVTRKENKGRINHGQPFEQNFIVNAPQLWSPESPALYKAVSKVYADNQLVDTYSTRFGIRSIEYIADKGFYLNGKH
RKFQGVCNHHDLGPLGAAINVAALRHQLTLLKDMGCDAIRTSHNMPAPELVELCDEMGFMMMIEPFDEWDIAKCENGYHR
YFNEWAERDMVNMLHNYRNNPCVVMWSIGNEVPTQCSPVGYKVAKFLQDICHREDPTRPVTCGMDQVTCVLANGFAAMID
IPGLNYRANRYQEAYDKLPQNLILGSETASTVSSRGVYKFPVKDKKSAQYDDHQCSSYDVEACSWSNIPDEDFALADDHH
WTIGQFVWTGFDYLGEPSPYDTDAWPNHSSMFGIIDLASLPKDRYYLYRSIWNKEAETLHILPHWTWPGREGEVTPVFVY
TNYPAAELFINGKSYGKQSKNNSSLKSRYRLMWMDAIYEPGEVKVVAYDKDGKAVAEKSVRTASKPHHIELVSNRNELTA
DGKDLAYVTVKVVDKDGNLCPADSRLINFSVKGAGKYRAGANGDPTSLDLFHLPKMHAFNGMLTAIVQTNETAGEIILTA
KAGGVKAGSIRLLTK

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=596, Percent_Identity=24.3288590604027, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI48994920, Length=388, Percent_Identity=26.8041237113402, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1787903, Length=448, Percent_Identity=24.3303571428571, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1786539, Length=440, Percent_Identity=24.5454545454545, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17510825, Length=285, Percent_Identity=26.3157894736842, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24651745, Length=521, Percent_Identity=26.295585412668, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI62471735, Length=446, Percent_Identity=26.9058295964126, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI19922582, Length=446, Percent_Identity=26.9058295964126, Blast_Score=119, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24655438, Length=446, Percent_Identity=26.9058295964126, Blast_Score=119, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 91971; Mature: 91971

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMKHQKQLFTVLLMSAACMLQAQRSETLLEKNWKFSKGDFPEAAQTDYNDTKWESVVIP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCEEEEEC
HDWAIFGPFDMNNDLQNVAVTQNFEKKASLKTGRTGGLPYVGTGWYRTSFDVPADKEVTL
CCCEEECCCCCCCCCCCEEEECCCHHHHCCCCCCCCCCCCEECCEEEEECCCCCCCEEEE
LFDGAMSEARVYVNGQEACFWPFGYNSFHCNVTPFLNKDGKNNTLAVRLENRPQSSRWYP
EEECCCCCCEEEECCCCEEEEECCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCC
GAGLYRNVHLIVTEKVHIPVWGTQITTPHVSDEFAAVRLQTKIENAGEKTEIRIETEILS
CCCCEEEEEEEEEEEEEEEEECCEECCCCCCCCEEEEEEEEHHCCCCCCEEEEEEEEEEC
PDGKVVTRKENKGRINHGQPFEQNFIVNAPQLWSPESPALYKAVSKVYADNQLVDTYSTR
CCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHH
FGIRSIEYIADKGFYLNGKHRKFQGVCNHHDLGPLGAAINVAALRHQLTLLKDMGCDAIR
CCCCCEEEHHCCCEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHH
TSHNMPAPELVELCDEMGFMMMIEPFDEWDIAKCENGYHRYFNEWAERDMVNMLHNYRNN
HCCCCCCHHHHHHHHHCCEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
PCVVMWSIGNEVPTQCSPVGYKVAKFLQDICHREDPTRPVTCGMDQVTCVLANGFAAMID
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEECCEEEEEE
IPGLNYRANRYQEAYDKLPQNLILGSETASTVSSRGVYKFPVKDKKSAQYDDHQCSSYDV
CCCCCCCHHHHHHHHHHCCHHEEECCCHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCC
EACSWSNIPDEDFALADDHHWTIGQFVWTGFDYLGEPSPYDTDAWPNHSSMFGIIDLASL
CCCCCCCCCCCCCEECCCCCEEHHHHHHHCHHHCCCCCCCCCCCCCCCCCEEEEEEHHHC
PKDRYYLYRSIWNKEAETLHILPHWTWPGREGEVTPVFVYTNYPAAELFINGKSYGKQSK
CCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEECCHHCCCCCC
NNSSLKSRYRLMWMDAIYEPGEVKVVAYDKDGKAVAEKSVRTASKPHHIELVSNRNELTA
CCHHHHHHHEEEEEHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCCCC
DGKDLAYVTVKVVDKDGNLCPADSRLINFSVKGAGKYRAGANGDPTSLDLFHLPKMHAFN
CCCCEEEEEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHC
GMLTAIVQTNETAGEIILTAKAGGVKAGSIRLLTK
CEEEEEEECCCCCCCEEEEEECCCCCCCCEEEEEC
>Mature Secondary Structure
MNMKHQKQLFTVLLMSAACMLQAQRSETLLEKNWKFSKGDFPEAAQTDYNDTKWESVVIP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCEEEEEC
HDWAIFGPFDMNNDLQNVAVTQNFEKKASLKTGRTGGLPYVGTGWYRTSFDVPADKEVTL
CCCEEECCCCCCCCCCCEEEECCCHHHHCCCCCCCCCCCCEECCEEEEECCCCCCCEEEE
LFDGAMSEARVYVNGQEACFWPFGYNSFHCNVTPFLNKDGKNNTLAVRLENRPQSSRWYP
EEECCCCCCEEEECCCCEEEEECCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCC
GAGLYRNVHLIVTEKVHIPVWGTQITTPHVSDEFAAVRLQTKIENAGEKTEIRIETEILS
CCCCEEEEEEEEEEEEEEEEECCEECCCCCCCCEEEEEEEEHHCCCCCCEEEEEEEEEEC
PDGKVVTRKENKGRINHGQPFEQNFIVNAPQLWSPESPALYKAVSKVYADNQLVDTYSTR
CCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHH
FGIRSIEYIADKGFYLNGKHRKFQGVCNHHDLGPLGAAINVAALRHQLTLLKDMGCDAIR
CCCCCEEEHHCCCEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHH
TSHNMPAPELVELCDEMGFMMMIEPFDEWDIAKCENGYHRYFNEWAERDMVNMLHNYRNN
HCCCCCCHHHHHHHHHCCEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
PCVVMWSIGNEVPTQCSPVGYKVAKFLQDICHREDPTRPVTCGMDQVTCVLANGFAAMID
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEECCEEEEEE
IPGLNYRANRYQEAYDKLPQNLILGSETASTVSSRGVYKFPVKDKKSAQYDDHQCSSYDV
CCCCCCCHHHHHHHHHHCCHHEEECCCHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCC
EACSWSNIPDEDFALADDHHWTIGQFVWTGFDYLGEPSPYDTDAWPNHSSMFGIIDLASL
CCCCCCCCCCCCCEECCCCCEEHHHHHHHCHHHCCCCCCCCCCCCCCCCCEEEEEEHHHC
PKDRYYLYRSIWNKEAETLHILPHWTWPGREGEVTPVFVYTNYPAAELFINGKSYGKQSK
CCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEECCHHCCCCCC
NNSSLKSRYRLMWMDAIYEPGEVKVVAYDKDGKAVAEKSVRTASKPHHIELVSNRNELTA
CCHHHHHHHEEEEEHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCCCC
DGKDLAYVTVKVVDKDGNLCPADSRLINFSVKGAGKYRAGANGDPTSLDLFHLPKMHAFN
CCCCEEEEEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHC
GMLTAIVQTNETAGEIILTAKAGGVKAGSIRLLTK
CEEEEEEECCCCCCCEEEEEECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA