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The map label for this gene is trpB2 [H]

Identifier: 161511186

GI number: 161511186

Start: 6111641

End: 6113011

Strand: Reverse

Name: trpB2 [H]

Synonym: BT_4664

Alternate gene names: 161511186

Gene position: 6113011-6111641 (Counterclockwise)

Preceding gene: 29350075

Following gene: 29350071

Centisome position: 97.65

GC content: 49.45

Gene sequence:

>1371_bases
ATGAGTGACAAAAGAAAACGGTATATCCTACCGGAAGAAGAAATTCCACATTACTGGTACAACATTCAGGCTGATATGGT
GAACAAGCCAATGCCGCCGTTGCACCCGGGAACAAAACAGCCGCTGAAAGCGGAAGATCTGTATCCAATCTTTGCCAAAG
AACTGTGTCATCAGGAGCTTAACCAGACTGACGCATGGATCGAAATACCGGAAGATGTACGCGAAATGTATAAATATTAT
CGCAGTACGCCGCTTGTACGCGCTTACGGACTGGAAAAAGCGCTCGGAACTCCGGCACATATCTATTTCAAGAACGAAAG
TGTCAGCCCGATCGGTTCTCATAAACTGAACTCTGCGCTGGCTCAGGCATATTATTGCAAAGAAGAAGGCGTCACGAACG
TTACTACCGAAACCGGTGCGGGCCAATGGGGAGCCGCCCTTTCTTATGCTGCCAAAGTTTTTGGTCTCGAAGCCGCTGTT
TATCAGGTAAAGATCAGCTACGAACAAAAGCCTTACCGCCGCAGCATTATGCAGACTTTCGGCGCGCAGGTCACTCCTTC
TCCGTCTATGTCTACCCGTGCGGGAAAAGATATCCTGACGGCTCACCCCACCTATCAGGGTTCTCTGGGTACAGCCATCT
CGGAAGCTATCGAACTTGCACAAATGACTCCCAACTGTAAATACACTCTTGGTTCGGTACTCAGCCACGTTACTCTCCAC
CAAACCATTATCGGTCTGGAAGCTGAAAAGCAAATGGAAATGGCGGGCGAATATCCGGACGTAGTAATCGGTTGTTTCGG
AGGCGGTTCCAACTTCGGAGGTATCTCTTTCCCATTCATGCGTCACAACATCCTGGAAGGAAAGAAAACGCGTTTCGTTG
CTGCCGAACCTGCTTCGTGCCCGAAGCTGACCCGCGGTAAATTCCAATATGACTTCGGTGATGAAGCCGGATATACTCCG
CTGCTCCCGATGTTTACATTAGGACACAACTTCGCTCCTGCCCATATCCATGCAGGCGGTCTTCGCTATCACGGTGCAGG
CGTCATCGTCTCCCAATTGCTGAAAGACAATCTGATGGAAGCGGTAGATATCCAGCAACTGGAATCTTTCGAAGCCGGCT
GTCTGTTCGCACAGGCGGAAGGTATTATTCCGGCACCGGAATCCAGCCACGCGATTGCTGCCGCTATACGCGAAGCAAAG
GCGTGCAAGGAAACAGGAGAAGAGAAAGTGATTCTGTTCAACCTGTCCGGACACGGTCTGATCGATATGGCATCTTATGA
CAAATATCTGGCAGGCGATTTAGTGAACTACGCACTGACTGACGAAGATATTCAGAAGAATCTGGATGAAATAGGAGACT
TATCCAAGTAA

Upstream 100 bases:

>100_bases
CTAAAATAACAATCGAATTCGATTCTTTGTTGACAAAATTACACGAATATTAAAAATTTTGCCTACTTTTGGCAGCAGAA
AACTAATAAACCACAATAAC

Downstream 100 bases:

>100_bases
GCCCCGGTTTCTGAACCGAATTCTGTAAGCATAAAAGCCCGGTATGATGCCGGGCTTATTTATTATCTCTACGAAACTAT
CCAAAAGAATACAGTCTTTT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSDKRKRYILPEEEIPHYWYNIQADMVNKPMPPLHPGTKQPLKAEDLYPIFAKELCHQELNQTDAWIEIPEDVREMYKYY
RSTPLVRAYGLEKALGTPAHIYFKNESVSPIGSHKLNSALAQAYYCKEEGVTNVTTETGAGQWGAALSYAAKVFGLEAAV
YQVKISYEQKPYRRSIMQTFGAQVTPSPSMSTRAGKDILTAHPTYQGSLGTAISEAIELAQMTPNCKYTLGSVLSHVTLH
QTIIGLEAEKQMEMAGEYPDVVIGCFGGGSNFGGISFPFMRHNILEGKKTRFVAAEPASCPKLTRGKFQYDFGDEAGYTP
LLPMFTLGHNFAPAHIHAGGLRYHGAGVIVSQLLKDNLMEAVDIQQLESFEAGCLFAQAEGIIPAPESSHAIAAAIREAK
ACKETGEEKVILFNLSGHGLIDMASYDKYLAGDLVNYALTDEDIQKNLDEIGDLSK

Sequences:

>Translated_456_residues
MSDKRKRYILPEEEIPHYWYNIQADMVNKPMPPLHPGTKQPLKAEDLYPIFAKELCHQELNQTDAWIEIPEDVREMYKYY
RSTPLVRAYGLEKALGTPAHIYFKNESVSPIGSHKLNSALAQAYYCKEEGVTNVTTETGAGQWGAALSYAAKVFGLEAAV
YQVKISYEQKPYRRSIMQTFGAQVTPSPSMSTRAGKDILTAHPTYQGSLGTAISEAIELAQMTPNCKYTLGSVLSHVTLH
QTIIGLEAEKQMEMAGEYPDVVIGCFGGGSNFGGISFPFMRHNILEGKKTRFVAAEPASCPKLTRGKFQYDFGDEAGYTP
LLPMFTLGHNFAPAHIHAGGLRYHGAGVIVSQLLKDNLMEAVDIQQLESFEAGCLFAQAEGIIPAPESSHAIAAAIREAK
ACKETGEEKVILFNLSGHGLIDMASYDKYLAGDLVNYALTDEDIQKNLDEIGDLSK
>Mature_455_residues
SDKRKRYILPEEEIPHYWYNIQADMVNKPMPPLHPGTKQPLKAEDLYPIFAKELCHQELNQTDAWIEIPEDVREMYKYYR
STPLVRAYGLEKALGTPAHIYFKNESVSPIGSHKLNSALAQAYYCKEEGVTNVTTETGAGQWGAALSYAAKVFGLEAAVY
QVKISYEQKPYRRSIMQTFGAQVTPSPSMSTRAGKDILTAHPTYQGSLGTAISEAIELAQMTPNCKYTLGSVLSHVTLHQ
TIIGLEAEKQMEMAGEYPDVVIGCFGGGSNFGGISFPFMRHNILEGKKTRFVAAEPASCPKLTRGKFQYDFGDEAGYTPL
LPMFTLGHNFAPAHIHAGGLRYHGAGVIVSQLLKDNLMEAVDIQQLESFEAGCLFAQAEGIIPAPESSHAIAAAIREAKA
CKETGEEKVILFNLSGHGLIDMASYDKYLAGDLVNYALTDEDIQKNLDEIGDLSK

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=381, Percent_Identity=28.0839895013123, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321412, Length=425, Percent_Identity=29.8823529411765, Blast_Score=119, Evalue=1e-27,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 50234; Mature: 50102

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKRKRYILPEEEIPHYWYNIQADMVNKPMPPLHPGTKQPLKAEDLYPIFAKELCHQEL
CCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NQTDAWIEIPEDVREMYKYYRSTPLVRAYGLEKALGTPAHIYFKNESVSPIGSHKLNSAL
CCCCCEEECCHHHHHHHHHHHCCCCEEHHCHHHHCCCCEEEEECCCCCCCCCHHHHHHHH
AQAYYCKEEGVTNVTTETGAGQWGAALSYAAKVFGLEAAVYQVKISYEQKPYRRSIMQTF
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHH
GAQVTPSPSMSTRAGKDILTAHPTYQGSLGTAISEAIELAQMTPNCKYTLGSVLSHVTLH
CCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
QTIIGLEAEKQMEMAGEYPDVVIGCFGGGSNFGGISFPFMRHNILEGKKTRFVAAEPASC
HHHHCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCC
PKLTRGKFQYDFGDEAGYTPLLPMFTLGHNFAPAHIHAGGLRYHGAGVIVSQLLKDNLME
CCCCCCCEEECCCCCCCCCCCHHHHHCCCCCCCCEEECCCEEECCHHHHHHHHHHHHHHH
AVDIQQLESFEAGCLFAQAEGIIPAPESSHAIAAAIREAKACKETGEEKVILFNLSGHGL
HHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCE
IDMASYDKYLAGDLVNYALTDEDIQKNLDEIGDLSK
EEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDKRKRYILPEEEIPHYWYNIQADMVNKPMPPLHPGTKQPLKAEDLYPIFAKELCHQEL
CCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NQTDAWIEIPEDVREMYKYYRSTPLVRAYGLEKALGTPAHIYFKNESVSPIGSHKLNSAL
CCCCCEEECCHHHHHHHHHHHCCCCEEHHCHHHHCCCCEEEEECCCCCCCCCHHHHHHHH
AQAYYCKEEGVTNVTTETGAGQWGAALSYAAKVFGLEAAVYQVKISYEQKPYRRSIMQTF
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHH
GAQVTPSPSMSTRAGKDILTAHPTYQGSLGTAISEAIELAQMTPNCKYTLGSVLSHVTLH
CCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
QTIIGLEAEKQMEMAGEYPDVVIGCFGGGSNFGGISFPFMRHNILEGKKTRFVAAEPASC
HHHHCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCC
PKLTRGKFQYDFGDEAGYTPLLPMFTLGHNFAPAHIHAGGLRYHGAGVIVSQLLKDNLME
CCCCCCCEEECCCCCCCCCCCHHHHHCCCCCCCCEEECCCEEECCHHHHHHHHHHHHHHH
AVDIQQLESFEAGCLFAQAEGIIPAPESSHAIAAAIREAKACKETGEEKVILFNLSGHGL
HHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCE
IDMASYDKYLAGDLVNYALTDEDIQKNLDEIGDLSK
EEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]