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The map label for this gene is pit [H]

Identifier: 29350045

GI number: 29350045

Start: 6072867

End: 6073886

Strand: Reverse

Name: pit [H]

Synonym: BT_4637

Alternate gene names: 29350045

Gene position: 6073886-6072867 (Counterclockwise)

Preceding gene: 29350046

Following gene: 29350044

Centisome position: 97.02

GC content: 44.22

Gene sequence:

>1020_bases
ATGGAATTATTAGTGACTATCATTATACTAGCTCTGATATTCGATTATATCAATGGCTTTCATGACGCAGCAAACTCGAT
CGCTACCATCGTATCGACTAAAGTACTGACTCCTTTTCAAGCGGTATTGTGGGCAGCATTCTTTAACTTCGTTGCGTTCT
TCATAGCCAAATACATTATAGGGGGCTTTGGCATAGCAAACACTGTGTCAAAGACAGTTGTTGAACAATACATCACGCTG
CCTATTATCCTGGCGGGCATTATTGCCGCTATTTTCTGGAATCTGATTACCTGGTGGAAGGGTATCCCGTCTTCTTCCTC
CCATACGCTTATAGGTGGTTTCGCAGGAGCTGCTATCATGGCAAACGGCTTTGAAGCTATTCAGCTCAATATCATTCTTA
AGATTGCCGCTTTTATCTTCCTGGCTCCATTTATCGGTATGATTATCGCTTTCGGATTTACTCTACTTGTGCTCTATATC
TGCCGACGTACAAATCCGCATACCGCTGAAATGTGGTTCAAGAGATTACAGTTGGTTTCTTCCGCCATGTTCAGTGTAGG
ACATGGTCTGAATGACTCGCAGAAGGTAATGGGTATTATCGCCGCCGCAATGATAGCTGCCCACTCTATGGGTCTGGGAA
TGGGAATAAACAGTATTTCTGATCTGCCGGACTGGGTAGCGTTCTCCTGTTTTACTGCTATCTCACTGGGAACGATGTCC
GGAGGATGGAAAATCGTAAAAACGATGGGAACCAGAATCACTAAAGTTACTCCATTGGAAGGTATGGTTGCCGAAACCGC
AGGTGCTTTTACTCTTTATATCACCGAAATGCTGAAAATACCTGTTAGTACGACTCATACAATTACCGGAGCAATCATTG
GTGTCGGAGCAACCAAACGTTTATCCGCTGTTCGCTGGGGGGTGACTAAAAGCCTCATGACTGCGTGGGTACTTACTATC
CCTGTCAGTGGACTCCTAGCTGCATGTATTTATTGTGTAGTTTCTCTGTTCCTGAAATAA

Upstream 100 bases:

>100_bases
ATCATGCATGAACTGGAAAAGACAACCGATGCAGCAGAACATGTAGGGAAAATATTAAAAAACCTGATCGTAAAGTATTC
ATAAAAGCAGAGCAAGACAC

Downstream 100 bases:

>100_bases
ATTCTGCTTCAAGGGAAACAACTCGAAATTTAAACAAGAAAAATAGCTACTCTTAACATCCCTCTACAGTATTTTGTAGA
GGGAATACTATAAAATGATA

Product: putative inorganic phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MELLVTIIILALIFDYINGFHDAANSIATIVSTKVLTPFQAVLWAAFFNFVAFFIAKYIIGGFGIANTVSKTVVEQYITL
PIILAGIIAAIFWNLITWWKGIPSSSSHTLIGGFAGAAIMANGFEAIQLNIILKIAAFIFLAPFIGMIIAFGFTLLVLYI
CRRTNPHTAEMWFKRLQLVSSAMFSVGHGLNDSQKVMGIIAAAMIAAHSMGLGMGINSISDLPDWVAFSCFTAISLGTMS
GGWKIVKTMGTRITKVTPLEGMVAETAGAFTLYITEMLKIPVSTTHTITGAIIGVGATKRLSAVRWGVTKSLMTAWVLTI
PVSGLLAACIYCVVSLFLK

Sequences:

>Translated_339_residues
MELLVTIIILALIFDYINGFHDAANSIATIVSTKVLTPFQAVLWAAFFNFVAFFIAKYIIGGFGIANTVSKTVVEQYITL
PIILAGIIAAIFWNLITWWKGIPSSSSHTLIGGFAGAAIMANGFEAIQLNIILKIAAFIFLAPFIGMIIAFGFTLLVLYI
CRRTNPHTAEMWFKRLQLVSSAMFSVGHGLNDSQKVMGIIAAAMIAAHSMGLGMGINSISDLPDWVAFSCFTAISLGTMS
GGWKIVKTMGTRITKVTPLEGMVAETAGAFTLYITEMLKIPVSTTHTITGAIIGVGATKRLSAVRWGVTKSLMTAWVLTI
PVSGLLAACIYCVVSLFLK
>Mature_339_residues
MELLVTIIILALIFDYINGFHDAANSIATIVSTKVLTPFQAVLWAAFFNFVAFFIAKYIIGGFGIANTVSKTVVEQYITL
PIILAGIIAAIFWNLITWWKGIPSSSSHTLIGGFAGAAIMANGFEAIQLNIILKIAAFIFLAPFIGMIIAFGFTLLVLYI
CRRTNPHTAEMWFKRLQLVSSAMFSVGHGLNDSQKVMGIIAAAMIAAHSMGLGMGINSISDLPDWVAFSCFTAISLGTMS
GGWKIVKTMGTRITKVTPLEGMVAETAGAFTLYITEMLKIPVSTTHTITGAIIGVGATKRLSAVRWGVTKSLMTAWVLTI
PVSGLLAACIYCVVSLFLK

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI5803173, Length=158, Percent_Identity=32.2784810126582, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI31543630, Length=158, Percent_Identity=30.379746835443, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1789360, Length=235, Percent_Identity=28.5106382978723, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789907, Length=235, Percent_Identity=30.6382978723404, Blast_Score=91, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI25146401, Length=172, Percent_Identity=27.3255813953488, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17539280, Length=178, Percent_Identity=26.9662921348315, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71981576, Length=173, Percent_Identity=26.5895953757225, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17536725, Length=168, Percent_Identity=25.5952380952381, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI32566716, Length=167, Percent_Identity=28.1437125748503, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319773, Length=178, Percent_Identity=28.6516853932584, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 36435; Mature: 36435

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLVTIIILALIFDYINGFHDAANSIATIVSTKVLTPFQAVLWAAFFNFVAFFIAKYII
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGFGIANTVSKTVVEQYITLPIILAGIIAAIFWNLITWWKGIPSSSSHTLIGGFAGAAIM
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHH
ANGFEAIQLNIILKIAAFIFLAPFIGMIIAFGFTLLVLYICRRTNPHTAEMWFKRLQLVS
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SAMFSVGHGLNDSQKVMGIIAAAMIAAHSMGLGMGINSISDLPDWVAFSCFTAISLGTMS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHCCC
GGWKIVKTMGTRITKVTPLEGMVAETAGAFTLYITEMLKIPVSTTHTITGAIIGVGATKR
CCHHHHHHHCCHHEEECCCHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHEECCCHHH
LSAVRWGVTKSLMTAWVLTIPVSGLLAACIYCVVSLFLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELLVTIIILALIFDYINGFHDAANSIATIVSTKVLTPFQAVLWAAFFNFVAFFIAKYII
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGFGIANTVSKTVVEQYITLPIILAGIIAAIFWNLITWWKGIPSSSSHTLIGGFAGAAIM
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHH
ANGFEAIQLNIILKIAAFIFLAPFIGMIIAFGFTLLVLYICRRTNPHTAEMWFKRLQLVS
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SAMFSVGHGLNDSQKVMGIIAAAMIAAHSMGLGMGINSISDLPDWVAFSCFTAISLGTMS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHCCC
GGWKIVKTMGTRITKVTPLEGMVAETAGAFTLYITEMLKIPVSTTHTITGAIIGVGATKR
CCHHHHHHHCCHHEEECCCHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHEECCCHHH
LSAVRWGVTKSLMTAWVLTIPVSGLLAACIYCVVSLFLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]