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The map label for this gene is murF [H]

Identifier: 29349052

GI number: 29349052

Start: 4721029

End: 4722327

Strand: Reverse

Name: murF [H]

Synonym: BT_3644

Alternate gene names: 29349052

Gene position: 4722327-4721029 (Counterclockwise)

Preceding gene: 29349053

Following gene: 29349050

Centisome position: 75.43

GC content: 48.65

Gene sequence:

>1299_bases
ATGAAACTTTCTGCTCTTTACCAGATATTTCTGGATTGCCAATTAGTGACGACCGATAGTCGGAACTGTCCTGAAGGATC
ACTGTTTATCGCTCTGAAAGGCGAGTCATTCAATGGAAACGCTTTTGCCGGAAAAGCATTGGAAACCGGTTGCGCCTATG
CTGTCATCGACGAATCCGAATACGCGATTGAAGGCGATCAACGATATATACTCGTAGACGACTGCCTGCAAACATTGCAG
CAGCTTGCCAATTACCACCGCCGCCAGCTGGGTACACGAGTAATCGGTATCACTGGTACCAACGGCAAGACGACTACGAA
GGAACTGATCTCCGCCGTACTCTCTCAGAGCCACAACATTCTTTATACGCTAGGTAATCTGAACAACCACATCGGCGTGC
CCTCTACCCTGCTCCGCCTGAAAGCGGAACACGACCTTGCCGTCATCGAGATGGGCGCCAATCATCCGGGAGAAATCAAA
TTCCTCAGCGAAATCGCAGAGCCGGACTGCGGTATCATCACCAATGTAGGCAAAGCCCATTTGGAAGGCTTCGGTTCTTT
CGAAGGTGTGATAAAAACCAAAGGAGAACTTTATGACTTTCTCCGTAAGAAAGAAGGTTCTACCGTCTTTATCCATCACG
ACAATGCTTATCTGATGAACATTGCCGAAGGACTGAACCTGATCCCTTACGGCACCGAAGATGACCTTTACGTAAACGGA
CGAATCACCGGCAACTCCCCTTACCTCACTTTCGAATGGAAAGCGGGCAAGGATGGTAAATCCTATCAGGTTCAGACGCA
ACTGATCGGAGAATACAACTTCCCCAATGCACTGGCTGCCATCACCATCGGCCGCTTCTTCGGAGTGGAAGATGCAAAGA
TCAACGAAGCCTTAGCCGGATATACACCACAGAACAACCGCTCGCAACTAAAGAAAACGGACGATAACACACTGATCATC
GACGCATACAACGCCAATCCGACCAGCATGATGGCTGCCTTACAGAACTTCCGCAACATGGAAGTTCCTCACAAAATGCT
CATCCTTGGTGATATGCGCGAACTGGGTGCCGAAAGCGCTGCCGAACATCAGAAGATAGCAGACTACCTCAAAGAATGCG
CGTTCGAAAAAGTATGGCTGGTAGGAGATCAGTTTGCAGCAGCAGAGCACTCCTTCCAAACCTATCCCAACGTACAGGAA
GTAATAAAGGAACTGGAAGCCGACAAGCCGAAAGGATACACTATCCTGATCAAAGGGTCCAACGGAATTAAGCTCAGTTC
GGTTGTAGATTATCTATAA

Upstream 100 bases:

>100_bases
CTCTTCCTTTGGTATATGCTGAGTAAAAAACGCTTCTGACCGATACCAAATCCGAAAGAATAGCCTATCTTTGCAACATT
TAAAAATAAAAGCTCAGGCT

Downstream 100 bases:

>100_bases
TATCGCTGTTAATTTCTCAAAGTATTCTCATGCCTTTGAGAAAAAATTCTCACCACGATGAGAAAAAATACTCATGCCTA
TGAGAATATTTTCTCACTGC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKLSALYQIFLDCQLVTTDSRNCPEGSLFIALKGESFNGNAFAGKALETGCAYAVIDESEYAIEGDQRYILVDDCLQTLQ
QLANYHRRQLGTRVIGITGTNGKTTTKELISAVLSQSHNILYTLGNLNNHIGVPSTLLRLKAEHDLAVIEMGANHPGEIK
FLSEIAEPDCGIITNVGKAHLEGFGSFEGVIKTKGELYDFLRKKEGSTVFIHHDNAYLMNIAEGLNLIPYGTEDDLYVNG
RITGNSPYLTFEWKAGKDGKSYQVQTQLIGEYNFPNALAAITIGRFFGVEDAKINEALAGYTPQNNRSQLKKTDDNTLII
DAYNANPTSMMAALQNFRNMEVPHKMLILGDMRELGAESAAEHQKIADYLKECAFEKVWLVGDQFAAAEHSFQTYPNVQE
VIKELEADKPKGYTILIKGSNGIKLSSVVDYL

Sequences:

>Translated_432_residues
MKLSALYQIFLDCQLVTTDSRNCPEGSLFIALKGESFNGNAFAGKALETGCAYAVIDESEYAIEGDQRYILVDDCLQTLQ
QLANYHRRQLGTRVIGITGTNGKTTTKELISAVLSQSHNILYTLGNLNNHIGVPSTLLRLKAEHDLAVIEMGANHPGEIK
FLSEIAEPDCGIITNVGKAHLEGFGSFEGVIKTKGELYDFLRKKEGSTVFIHHDNAYLMNIAEGLNLIPYGTEDDLYVNG
RITGNSPYLTFEWKAGKDGKSYQVQTQLIGEYNFPNALAAITIGRFFGVEDAKINEALAGYTPQNNRSQLKKTDDNTLII
DAYNANPTSMMAALQNFRNMEVPHKMLILGDMRELGAESAAEHQKIADYLKECAFEKVWLVGDQFAAAEHSFQTYPNVQE
VIKELEADKPKGYTILIKGSNGIKLSSVVDYL
>Mature_432_residues
MKLSALYQIFLDCQLVTTDSRNCPEGSLFIALKGESFNGNAFAGKALETGCAYAVIDESEYAIEGDQRYILVDDCLQTLQ
QLANYHRRQLGTRVIGITGTNGKTTTKELISAVLSQSHNILYTLGNLNNHIGVPSTLLRLKAEHDLAVIEMGANHPGEIK
FLSEIAEPDCGIITNVGKAHLEGFGSFEGVIKTKGELYDFLRKKEGSTVFIHHDNAYLMNIAEGLNLIPYGTEDDLYVNG
RITGNSPYLTFEWKAGKDGKSYQVQTQLIGEYNFPNALAAITIGRFFGVEDAKINEALAGYTPQNNRSQLKKTDDNTLII
DAYNANPTSMMAALQNFRNMEVPHKMLILGDMRELGAESAAEHQKIADYLKECAFEKVWLVGDQFAAAEHSFQTYPNVQE
VIKELEADKPKGYTILIKGSNGIKLSSVVDYL

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=442, Percent_Identity=36.1990950226244, Blast_Score=209, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 47730; Mature: 47730

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSALYQIFLDCQLVTTDSRNCPEGSLFIALKGESFNGNAFAGKALETGCAYAVIDESE
CCHHHHHHHHEEEEEEECCCCCCCCCCEEEEEECCCCCCCCEECCHHHCCCEEEEEECCC
YAIEGDQRYILVDDCLQTLQQLANYHRRQLGTRVIGITGTNGKTTTKELISAVLSQSHNI
EEEECCCEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCE
LYTLGNLNNHIGVPSTLLRLKAEHDLAVIEMGANHPGEIKFLSEIAEPDCGIITNVGKAH
EEEECCCCCCCCCCHHHHHEECCCCEEEEEECCCCCCCCHHHHHHCCCCCCEEECCCHHH
LEGFGSFEGVIKTKGELYDFLRKKEGSTVFIHHDNAYLMNIAEGLNLIPYGTEDDLYVNG
HCCCCCCCHHEECHHHHHHHHHHCCCCEEEEECCCEEEEEHHHCCEEEECCCCCCEEEEE
RITGNSPYLTFEWKAGKDGKSYQVQTQLIGEYNFPNALAAITIGRFFGVEDAKINEALAG
EEECCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHCC
YTPQNNRSQLKKTDDNTLIIDAYNANPTSMMAALQNFRNMEVPHKMLILGDMRELGAESA
CCCCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECHHHHCCHHH
AEHQKIADYLKECAFEKVWLVGDQFAAAEHSFQTYPNVQEVIKELEADKPKGYTILIKGS
HHHHHHHHHHHHHHHHEEEEECCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEEECC
NGIKLSSVVDYL
CCCCHHHHHHCC
>Mature Secondary Structure
MKLSALYQIFLDCQLVTTDSRNCPEGSLFIALKGESFNGNAFAGKALETGCAYAVIDESE
CCHHHHHHHHEEEEEEECCCCCCCCCCEEEEEECCCCCCCCEECCHHHCCCEEEEEECCC
YAIEGDQRYILVDDCLQTLQQLANYHRRQLGTRVIGITGTNGKTTTKELISAVLSQSHNI
EEEECCCEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCE
LYTLGNLNNHIGVPSTLLRLKAEHDLAVIEMGANHPGEIKFLSEIAEPDCGIITNVGKAH
EEEECCCCCCCCCCHHHHHEECCCCEEEEEECCCCCCCCHHHHHHCCCCCCEEECCCHHH
LEGFGSFEGVIKTKGELYDFLRKKEGSTVFIHHDNAYLMNIAEGLNLIPYGTEDDLYVNG
HCCCCCCCHHEECHHHHHHHHHHCCCCEEEEECCCEEEEEHHHCCEEEECCCCCCEEEEE
RITGNSPYLTFEWKAGKDGKSYQVQTQLIGEYNFPNALAAITIGRFFGVEDAKINEALAG
EEECCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHCC
YTPQNNRSQLKKTDDNTLIIDAYNANPTSMMAALQNFRNMEVPHKMLILGDMRELGAESA
CCCCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECHHHHCCHHH
AEHQKIADYLKECAFEKVWLVGDQFAAAEHSFQTYPNVQEVIKELEADKPKGYTILIKGS
HHHHHHHHHHHHHHHHEEEEECCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEEECC
NGIKLSSVVDYL
CCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]