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The map label for this gene is lepB [H]

Identifier: 29348728

GI number: 29348728

Start: 4262067

End: 4263548

Strand: Reverse

Name: lepB [H]

Synonym: BT_3319

Alternate gene names: 29348728

Gene position: 4263548-4262067 (Counterclockwise)

Preceding gene: 29348729

Following gene: 29348727

Centisome position: 68.1

GC content: 46.02

Gene sequence:

>1482_bases
ATGAGACAAGCCACACGCACACAATGGATCAAATGCACCATTGCTATTCTCCTTTATCTCGCCTTCCTCATCTGGGTACG
CAGTTGGTGGGGGCTGATTGTCGTTCCTTTCATCTTCGATATTTATATCACCAAGAAAATACCCTGGTCATTCTGGAAAA
AATCGAAGAATCCTACCGTTCGTAATGTTATGAGCTGGGTAGATGCCATTGTCTTTGCATTGGTTGCTGTCTACTTTGTC
AATATCTACATTTTCCAAAACTATCAGATTCCTTCCTCGTCTTTGGAAAAGTCATTGCTGGTAGGCGACTTCCTGTACGT
CAGTAAGATGAGCTACGGGCCACGTGTGCCGAATACGCCGCTTTCCATGCCGTTGGCGCAACATACGTTGCCTATTTTAA
ATACCAAATCATATATCGAATGGCCTCAGTGGAAATATAAAAGAGTGCCCGGGTTCGGAAAAGTGAAACTCAATGACATT
GTTGTCTTCAACTTTCCTGCCGGTGATACGGTAGCGCTCAACAATCAGCAAACAGATTTCTACTCTATAGCTTACGGTGA
AGGTCAGCGCCTGTATCCCAAGCAGATAGAGATGGACAGTCTGACACGCCAGCAGCAACGTGCTGTTTACGATTTGTATT
ATAATGCCGGTCGCCAGCAGATTCTATCGAATCCGCGTGTTTACGGAGAGGTCGTTTATCGTCCGGTAGACCGCCGTGAA
AACTATGTAAAACGCTGTGTCGGTCTGCCCGGAGATACGCTGCAGATCGTAGACGGTCAGGTCATGATTGACGGCAAGGC
TATCGAGAATCCCGAAAATCTGCAGTTCAACTACTTCGTACAGACCACAGGCCCGTATATCACAGAAGATATGTTCCGTG
AATTAGGCATCAGCAAGGCAGACCAGACATTGTATGATGATTCTTCATGGGAAGAAACCTTCAGACAGATCGGACTGGAC
AATCGGAATGCACAAGGAAAGATGGCTCCTATTTATCATCTTCCGCTGACGAAGAAAATGTACGAAACATTGTCCGGCAA
CAAAAAGTTCATCAGCAAGATCGTCATGGAACCGGAAGAGTATGCCGGACAGATGTACCCGCTCAACTTACATACCAAAT
GGAACCGTAACAACTACGGTCCGATCTGGATTCCCGCCAAAGGTGCTACTATCACTCTGACTGAAGATAACCTGCCTATC
TACGAACGTTGTATTGTCGCTTACGAAGGAAATAAACTGGAGATCAAGCCGGACGGTATTTATATCAACGGCGAGAAAAC
CGATCAATATACCTTTAAAATGGACTATTACTGGATGATGGGCGACAACCGCCACAATTCTGCGGACTCCCGCTATTGGG
GATTCGTTCCTGAAGACCATGTAGTAGGTAAACCTATCGTTGTATGGCTGTCACTTGACAAAGACCGCGGATGGTTTGAT
GGTAAAATCCGTTGGAACCGCCTCTTCAAGTGGGTAGACTAA

Upstream 100 bases:

>100_bases
CGAATACACGGCCCAACATGAGGGATATCTGGGCATGAGTGATTTATTCCCATTCTTAAACGAATAAGGCTTCAAAGCCA
CTATCAAACGAATATAGATT

Downstream 100 bases:

>100_bases
TAAGTATACAGACGTGATAGAGTGATAAGGTGATAGAAATTGATGAACATTCGTAAATTCAAATGGATATTCGCATTCGC
CGGAGCAATAGCCGTCGTGC

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MRQATRTQWIKCTIAILLYLAFLIWVRSWWGLIVVPFIFDIYITKKIPWSFWKKSKNPTVRNVMSWVDAIVFALVAVYFV
NIYIFQNYQIPSSSLEKSLLVGDFLYVSKMSYGPRVPNTPLSMPLAQHTLPILNTKSYIEWPQWKYKRVPGFGKVKLNDI
VVFNFPAGDTVALNNQQTDFYSIAYGEGQRLYPKQIEMDSLTRQQQRAVYDLYYNAGRQQILSNPRVYGEVVYRPVDRRE
NYVKRCVGLPGDTLQIVDGQVMIDGKAIENPENLQFNYFVQTTGPYITEDMFRELGISKADQTLYDDSSWEETFRQIGLD
NRNAQGKMAPIYHLPLTKKMYETLSGNKKFISKIVMEPEEYAGQMYPLNLHTKWNRNNYGPIWIPAKGATITLTEDNLPI
YERCIVAYEGNKLEIKPDGIYINGEKTDQYTFKMDYYWMMGDNRHNSADSRYWGFVPEDHVVGKPIVVWLSLDKDRGWFD
GKIRWNRLFKWVD

Sequences:

>Translated_493_residues
MRQATRTQWIKCTIAILLYLAFLIWVRSWWGLIVVPFIFDIYITKKIPWSFWKKSKNPTVRNVMSWVDAIVFALVAVYFV
NIYIFQNYQIPSSSLEKSLLVGDFLYVSKMSYGPRVPNTPLSMPLAQHTLPILNTKSYIEWPQWKYKRVPGFGKVKLNDI
VVFNFPAGDTVALNNQQTDFYSIAYGEGQRLYPKQIEMDSLTRQQQRAVYDLYYNAGRQQILSNPRVYGEVVYRPVDRRE
NYVKRCVGLPGDTLQIVDGQVMIDGKAIENPENLQFNYFVQTTGPYITEDMFRELGISKADQTLYDDSSWEETFRQIGLD
NRNAQGKMAPIYHLPLTKKMYETLSGNKKFISKIVMEPEEYAGQMYPLNLHTKWNRNNYGPIWIPAKGATITLTEDNLPI
YERCIVAYEGNKLEIKPDGIYINGEKTDQYTFKMDYYWMMGDNRHNSADSRYWGFVPEDHVVGKPIVVWLSLDKDRGWFD
GKIRWNRLFKWVD
>Mature_493_residues
MRQATRTQWIKCTIAILLYLAFLIWVRSWWGLIVVPFIFDIYITKKIPWSFWKKSKNPTVRNVMSWVDAIVFALVAVYFV
NIYIFQNYQIPSSSLEKSLLVGDFLYVSKMSYGPRVPNTPLSMPLAQHTLPILNTKSYIEWPQWKYKRVPGFGKVKLNDI
VVFNFPAGDTVALNNQQTDFYSIAYGEGQRLYPKQIEMDSLTRQQQRAVYDLYYNAGRQQILSNPRVYGEVVYRPVDRRE
NYVKRCVGLPGDTLQIVDGQVMIDGKAIENPENLQFNYFVQTTGPYITEDMFRELGISKADQTLYDDSSWEETFRQIGLD
NRNAQGKMAPIYHLPLTKKMYETLSGNKKFISKIVMEPEEYAGQMYPLNLHTKWNRNNYGPIWIPAKGATITLTEDNLPI
YERCIVAYEGNKLEIKPDGIYINGEKTDQYTFKMDYYWMMGDNRHNSADSRYWGFVPEDHVVGKPIVVWLSLDKDRGWFD
GKIRWNRLFKWVD

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=52, Percent_Identity=55.7692307692308, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR019533
- InterPro:   IPR019766 [H]

Pfam domain/function: PF00717 Peptidase_S24; PF10502 Peptidase_S26 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 57769; Mature: 57769

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQATRTQWIKCTIAILLYLAFLIWVRSWWGLIVVPFIFDIYITKKIPWSFWKKSKNPTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCCHHHHHCCCCCHH
RNVMSWVDAIVFALVAVYFVNIYIFQNYQIPSSSLEKSLLVGDFLYVSKMSYGPRVPNTP
HHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LSMPLAQHTLPILNTKSYIEWPQWKYKRVPGFGKVKLNDIVVFNFPAGDTVALNNQQTDF
CCCCHHHHCCCEECCCCCCCCCCCCEECCCCCCEEEEEEEEEEECCCCCEEEECCCCCCE
YSIAYGEGQRLYPKQIEMDSLTRQQQRAVYDLYYNAGRQQILSNPRVYGEVVYRPVDRRE
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEHEEECCHHHHH
NYVKRCVGLPGDTLQIVDGQVMIDGKAIENPENLQFNYFVQTTGPYITEDMFRELGISKA
HHHHHHHCCCCCEEEEECCEEEECCEECCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCC
DQTLYDDSSWEETFRQIGLDNRNAQGKMAPIYHLPLTKKMYETLSGNKKFISKIVMEPEE
CHHHCCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHCCCHH
YAGQMYPLNLHTKWNRNNYGPIWIPAKGATITLTEDNLPIYERCIVAYEGNKLEIKPDGI
HCCCEEEEEEEEEECCCCCCCEEEECCCCEEEEECCCCCHHHEEEEEEECCEEEEECCCE
YINGEKTDQYTFKMDYYWMMGDNRHNSADSRYWGFVPEDHVVGKPIVVWLSLDKDRGWFD
EECCCCCCEEEEEEEEEEEECCCCCCCCCCCEECCCCCCCCCCCCEEEEEEECCCCCEEC
GKIRWNRLFKWVD
CEEEEEHHHCCCC
>Mature Secondary Structure
MRQATRTQWIKCTIAILLYLAFLIWVRSWWGLIVVPFIFDIYITKKIPWSFWKKSKNPTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCCHHHHHCCCCCHH
RNVMSWVDAIVFALVAVYFVNIYIFQNYQIPSSSLEKSLLVGDFLYVSKMSYGPRVPNTP
HHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LSMPLAQHTLPILNTKSYIEWPQWKYKRVPGFGKVKLNDIVVFNFPAGDTVALNNQQTDF
CCCCHHHHCCCEECCCCCCCCCCCCEECCCCCCEEEEEEEEEEECCCCCEEEECCCCCCE
YSIAYGEGQRLYPKQIEMDSLTRQQQRAVYDLYYNAGRQQILSNPRVYGEVVYRPVDRRE
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEHEEECCHHHHH
NYVKRCVGLPGDTLQIVDGQVMIDGKAIENPENLQFNYFVQTTGPYITEDMFRELGISKA
HHHHHHHCCCCCEEEEECCEEEECCEECCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCC
DQTLYDDSSWEETFRQIGLDNRNAQGKMAPIYHLPLTKKMYETLSGNKKFISKIVMEPEE
CHHHCCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHCCCHH
YAGQMYPLNLHTKWNRNNYGPIWIPAKGATITLTEDNLPIYERCIVAYEGNKLEIKPDGI
HCCCEEEEEEEEEECCCCCCCEEEECCCCEEEEECCCCCHHHEEEEEEECCEEEEECCCE
YINGEKTDQYTFKMDYYWMMGDNRHNSADSRYWGFVPEDHVVGKPIVVWLSLDKDRGWFD
EECCCCCCEEEEEEEEEEEECCCCCCCCCCCEECCCCCCCCCCCCEEEEEEECCCCCEEC
GKIRWNRLFKWVD
CEEEEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]