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The map label for this gene is lepB [C]

Identifier: 29348727

GI number: 29348727

Start: 4261086

End: 4262024

Strand: Reverse

Name: lepB [C]

Synonym: BT_3318

Alternate gene names: 29348727

Gene position: 4262024-4261086 (Counterclockwise)

Preceding gene: 29348728

Following gene: 29348726

Centisome position: 68.08

GC content: 46.96

Gene sequence:

>939_bases
ATGAACATTCGTAAATTCAAATGGATATTCGCATTCGCCGGAGCAATAGCCGTCGTGCTTCTGCTCCGGGGATTCGCTTT
TACATCCTGCCTTATCCCTTCCACCGGTATGGAGAATTCACTTTTTCAGGGTGAACGTATTCTGGTCAACAAATGGAGCT
ACGGCCTGCGGGTTCCGCTGATGTCACTGTTCTCATATCACCGCTGGTGCGAGCGATCTGTCCGGCAACAAGATGTTGTA
GTCTTTAATAATCCCGCCGCCATCGGGCAACCGACTATTGACCGAAGAGAAATCTATATCAGCCGTTGTATCGGTACTCC
CGGTGATACCCTGCTTGTCGATTCCCTTTTCTCCGTATCCTCTCCCGAAGCGCAACTCAATCCGGACAAGAAAAGGCTCT
ACACCTATCCCGCTGCCAAAGAGCAGTTGATCACTTCGCTTATGCAAACCCTTTCCATCACCAACGACGGACTGATGGGA
AGTAATGACAGTACTCATGTCCGCAGTTTCAGCCGCTATGAATATTACTTACTGGAACAAGCGATCAGCGATCAGAACTG
GATTCAGCCTTTGACGGAAAAGAGCGAAAAAGAGCTTAAACCGCTCATCGTTCCCGGCAAAGGGAAAGCACTACGAGTAT
ATCCGTGGAATATCACATTGCTGCGAAATACCCTCGTGATGCACGAAGGCAAACAGGCGGAAATAAAGAATGATACGCTT
TATATCGACGGAAAACCCACACAGCATTGTTTTTTTACCAAAGATTACTACTGGATGGCTTCCAACAATAGTGTCAATCT
GTCCGATTCACGTCTGTTCGGATTCGTTCCGCAAGACCATATCATCGGGAAAGCATCGTTGATCTGGTTCTCTAAAGAAA
AAGGAACGGGAATCTTTGACGGATACCGATGGAATCGCTTCTTTCAGTCTGTAAAATAA

Upstream 100 bases:

>100_bases
CCGCGGATGGTTTGATGGTAAAATCCGTTGGAACCGCCTCTTCAAGTGGGTAGACTAATAAGTATACAGACGTGATAGAG
TGATAAGGTGATAGAAATTG

Downstream 100 bases:

>100_bases
TTCACATGCATAGTTTTCAACATAACATCTGTACATAAAATAATGAGAATAGCTTATTTATCTTCTGCCTACCTTGCTCC
AGTAGAATATTATACCAAAC

Product: signal peptidase I

Products: NA

Alternate protein names: 30S Ribosomal /Signal Peptidase I; Signal Peptidase; Exported Signal Peptidase I

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MNIRKFKWIFAFAGAIAVVLLLRGFAFTSCLIPSTGMENSLFQGERILVNKWSYGLRVPLMSLFSYHRWCERSVRQQDVV
VFNNPAAIGQPTIDRREIYISRCIGTPGDTLLVDSLFSVSSPEAQLNPDKKRLYTYPAAKEQLITSLMQTLSITNDGLMG
SNDSTHVRSFSRYEYYLLEQAISDQNWIQPLTEKSEKELKPLIVPGKGKALRVYPWNITLLRNTLVMHEGKQAEIKNDTL
YIDGKPTQHCFFTKDYYWMASNNSVNLSDSRLFGFVPQDHIIGKASLIWFSKEKGTGIFDGYRWNRFFQSVK

Sequences:

>Translated_312_residues
MNIRKFKWIFAFAGAIAVVLLLRGFAFTSCLIPSTGMENSLFQGERILVNKWSYGLRVPLMSLFSYHRWCERSVRQQDVV
VFNNPAAIGQPTIDRREIYISRCIGTPGDTLLVDSLFSVSSPEAQLNPDKKRLYTYPAAKEQLITSLMQTLSITNDGLMG
SNDSTHVRSFSRYEYYLLEQAISDQNWIQPLTEKSEKELKPLIVPGKGKALRVYPWNITLLRNTLVMHEGKQAEIKNDTL
YIDGKPTQHCFFTKDYYWMASNNSVNLSDSRLFGFVPQDHIIGKASLIWFSKEKGTGIFDGYRWNRFFQSVK
>Mature_312_residues
MNIRKFKWIFAFAGAIAVVLLLRGFAFTSCLIPSTGMENSLFQGERILVNKWSYGLRVPLMSLFSYHRWCERSVRQQDVV
VFNNPAAIGQPTIDRREIYISRCIGTPGDTLLVDSLFSVSSPEAQLNPDKKRLYTYPAAKEQLITSLMQTLSITNDGLMG
SNDSTHVRSFSRYEYYLLEQAISDQNWIQPLTEKSEKELKPLIVPGKGKALRVYPWNITLLRNTLVMHEGKQAEIKNDTL
YIDGKPTQHCFFTKDYYWMASNNSVNLSDSRLFGFVPQDHIIGKASLIWFSKEKGTGIFDGYRWNRFFQSVK

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.21.89

Molecular weight: Translated: 35843; Mature: 35843

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRKFKWIFAFAGAIAVVLLLRGFAFTSCLIPSTGMENSLFQGERILVNKWSYGLRVPL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHH
MSLFSYHRWCERSVRQQDVVVFNNPAAIGQPTIDRREIYISRCIGTPGDTLLVDSLFSVS
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHEEEEECCCCCCCHHHHHHHHCCC
SPEAQLNPDKKRLYTYPAAKEQLITSLMQTLSITNDGLMGSNDSTHVRSFSRYEYYLLEQ
CCCCCCCCCHHHEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AISDQNWIQPLTEKSEKELKPLIVPGKGKALRVYPWNITLLRNTLVMHEGKQAEIKNDTL
HHCCCHHHHHHHHHHHHCCCCEEECCCCCEEEEEECEEEEEEEEHEEECCCCCEECCCEE
YIDGKPTQHCFFTKDYYWMASNNSVNLSDSRLFGFVPQDHIIGKASLIWFSKEKGTGIFD
EECCCCCCEEEEECCEEEEECCCEECCCCCEEEECCCCCCEECCEEEEEEECCCCCCCCC
GYRWNRFFQSVK
CHHHHHHHHHCC
>Mature Secondary Structure
MNIRKFKWIFAFAGAIAVVLLLRGFAFTSCLIPSTGMENSLFQGERILVNKWSYGLRVPL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHH
MSLFSYHRWCERSVRQQDVVVFNNPAAIGQPTIDRREIYISRCIGTPGDTLLVDSLFSVS
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHEEEEECCCCCCCHHHHHHHHCCC
SPEAQLNPDKKRLYTYPAAKEQLITSLMQTLSITNDGLMGSNDSTHVRSFSRYEYYLLEQ
CCCCCCCCCHHHEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AISDQNWIQPLTEKSEKELKPLIVPGKGKALRVYPWNITLLRNTLVMHEGKQAEIKNDTL
HHCCCHHHHHHHHHHHHCCCCEEECCCCCEEEEEECEEEEEEEEHEEECCCCCEECCCEE
YIDGKPTQHCFFTKDYYWMASNNSVNLSDSRLFGFVPQDHIIGKASLIWFSKEKGTGIFD
EECCCCCCEEEEECCEEEEECCCEECCCCCEEEECCCCCCEECCEEEEEEECCCCCCCCC
GYRWNRFFQSVK
CHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phospholipids; Triton X-100 [C]

Metal ions: NA

Kcat value (1/min): 523.8 [C]

Specific activity: NA

Km value (mM): 0.52 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile}} 0.33 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile}} 0.032 {pro-OmpA-nuclease} 0.0165 {pro-OmpA-nuclease} 1 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile-CONH2}} [C]

Substrates: NA

Specific reaction: NA

General reaction: Peptide bond hydrolysis [C]

Inhibitor: 1-Ethyl -3-(3-dimethyl aminopropyl) carbodiimide; beta-Lactams; Bromosuccinimide; Carboxyphenanthroline; Cholate; Cu2+; Deoxycholate; Diethyl dicarbonate; Dinitrophenol; Hg2+; Mg2+ most serine peptidases; NEM Cys; Phenyl glyoxal; SDS; Sodiumchloride [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA