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The map label for this gene is mnmG

Identifier: 29348677

GI number: 29348677

Start: 4171817

End: 4173703

Strand: Reverse

Name: mnmG

Synonym: BT_3268

Alternate gene names: 29348677

Gene position: 4173703-4171817 (Counterclockwise)

Preceding gene: 29348692

Following gene: 29348676

Centisome position: 66.67

GC content: 49.23

Gene sequence:

>1887_bases
ATGGATTTTAAGTACGATGTAATTGTAATTGGTGCCGGACATGCAGGTTGTGAAGCGGCAGCCGCCGCTGCGAATTTAGG
TTCCAAAACTTGTCTGATCACAATGGACATGAACAAGATCGGACAGATGAGTTGCAATCCGGCAGTAGGAGGAATCGCCA
AAGGACAAATAGTACGCGAAATAGACGCACTGGGCGGACAGATGGGATTGGTGACGGATGAAACTGCTATCCAGTTCCGA
ATCCTGAACCGCTCGAAAGGTCCCGCCATGTGGAGTCCCCGCGCTCAATGCGACCGTGCCAAGTTTATCTGGTCATGGCG
TGAGAAACTGGAAAATACTCCCAACCTTCATATCTGGCAGGATACCGTCTGTGAATTGCTCGTTGAAAACGGTGAAGTAG
TCGGACTCGTCACTCTTTGGGGTGTCACATTCAAAGCGAAATGTATCGTACTGACCGCAGGAACATTCTTGAACGGACTC
ATGCACGTAGGCCGCCACCAATTGCCCGGCGGAAGAATGGCAGAACCCGCATCTTACCAGCTGACAGAATCCATCGCCCG
ACATGGCATTGCCTACGGAAGGATGAAGACCGGCACACCGGTACGAATCGACGCACGCAGCATCCACTTCGATCTGATGG
ATACACAAGACGGAGAATGCGACTTCCACAAGTTCTCATTCATGAATACCAGTACGCGCCATTTGAAGCAACTGCAATGC
TGGACCTGCTATACCAACGAAGAAGTGCACCGAATTCTGCGCGAAGGGCTGCCGGATTCTCCCCTGTTCAACGGACAGAT
TCAAAGTATCGGTCCGCGCTATTGCCCCAGCATCGAAACCAAGATTGTCACCTTCCCCGACAAAGAACAGCACCAACTGT
TCCTCGAACCCGAAGGTGAAACAACACAGGAATTATACCTGAACGGATTCTCTTCTTCTCTTCCGATGGATATTCAGATA
GCCGCGCTGAAAAAAGTACCGGCATTCAAGGATATCGTCATCTATCGTCCCGGATACGCGATTGAGTACGATTACTTCGA
CCCCACCCAATTAAAACATTCGCTGGAATCGAAGATTATCAAAAACCTGTTCTTTGCCGGGCAAGTGAACGGAACAACCG
GTTACGAAGAAGCGGGAGGACAAGGACTGATCGCCGGAATCAACGCACACATCAACTGTCACGGTGGTGAAGCCTTCACA
CTGGCTCGCGACGAAGCATATATCGGTGTATTGATAGACGACCTGGTGACCAAAGGAGTAGACGAACCATACCGTATGTT
CACTTCACGTGCCGAATACCGTATTCTTCTCCGCATGGACGACGCCGACATGAGACTGACCGAAAGAGCATATCACCTCG
GACTGGCAAGAGAGGATCGCTACCAATTAATGAAGACCAAGAAGGAGGCATTAGAGCAGATAGTAAATTTCGCAAAGAAC
TATTCGATGAAACCGGCATTAATTAATGATGCCCTCGAAAAGTTGGGAACAACTCCCCTTCGTCAAGGCTGTAAGCTGAT
CGAAATTCTCAATCGTCCGCAAATCACGATTGAGAATATCGCGGAACATGTCCCCGCCTTCCAAAGAGAATTAGAAAAAG
CAACCGCTGCAGACTCCGACCGTAAAGAAGAGATTCTGGAAGCGGCAGAAATACTGATCAAGTATCAAGGCTATATTGAC
AGAGAGCGAATGATAGCAGAAAAGCTGGCACGCCTGGAAAGCATCAAGATCAAAGGCAAATTTGATTATGCTTCCATCCA
GTCGCTATCTACGGAAGCTCGTCAGAAGCTAGTGAAGATTGATCCGGAAACGATTGCTCAAGCGAGCCGGATCCCGGGAG
TATCACCAAGCGACATCAACGTATTGCTGGTACTTTCCGGCCGATAA

Upstream 100 bases:

>100_bases
CCTGCCTCCTCCCCGAAATTTCGGAAGCAAAAACCGGCAGAAGATACAACTCTCGCAACTTTTCCGTAACTTTGCAGCGT
TTGATAATAAATAAGGTAGA

Downstream 100 bases:

>100_bases
GCGGCAACGTTTCACGTGAAACAAATCATTTAAAGAAACGAGATAAACCAATGATTATGAGCAAAGAAACGCTAATTAAA
AGCATCCGGGAAATTCCCGA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 628; Mature: 628

Protein sequence:

>628_residues
MDFKYDVIVIGAGHAGCEAAAAAANLGSKTCLITMDMNKIGQMSCNPAVGGIAKGQIVREIDALGGQMGLVTDETAIQFR
ILNRSKGPAMWSPRAQCDRAKFIWSWREKLENTPNLHIWQDTVCELLVENGEVVGLVTLWGVTFKAKCIVLTAGTFLNGL
MHVGRHQLPGGRMAEPASYQLTESIARHGIAYGRMKTGTPVRIDARSIHFDLMDTQDGECDFHKFSFMNTSTRHLKQLQC
WTCYTNEEVHRILREGLPDSPLFNGQIQSIGPRYCPSIETKIVTFPDKEQHQLFLEPEGETTQELYLNGFSSSLPMDIQI
AALKKVPAFKDIVIYRPGYAIEYDYFDPTQLKHSLESKIIKNLFFAGQVNGTTGYEEAGGQGLIAGINAHINCHGGEAFT
LARDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILLRMDDADMRLTERAYHLGLAREDRYQLMKTKKEALEQIVNFAKN
YSMKPALINDALEKLGTTPLRQGCKLIEILNRPQITIENIAEHVPAFQRELEKATAADSDRKEEILEAAEILIKYQGYID
RERMIAEKLARLESIKIKGKFDYASIQSLSTEARQKLVKIDPETIAQASRIPGVSPSDINVLLVLSGR

Sequences:

>Translated_628_residues
MDFKYDVIVIGAGHAGCEAAAAAANLGSKTCLITMDMNKIGQMSCNPAVGGIAKGQIVREIDALGGQMGLVTDETAIQFR
ILNRSKGPAMWSPRAQCDRAKFIWSWREKLENTPNLHIWQDTVCELLVENGEVVGLVTLWGVTFKAKCIVLTAGTFLNGL
MHVGRHQLPGGRMAEPASYQLTESIARHGIAYGRMKTGTPVRIDARSIHFDLMDTQDGECDFHKFSFMNTSTRHLKQLQC
WTCYTNEEVHRILREGLPDSPLFNGQIQSIGPRYCPSIETKIVTFPDKEQHQLFLEPEGETTQELYLNGFSSSLPMDIQI
AALKKVPAFKDIVIYRPGYAIEYDYFDPTQLKHSLESKIIKNLFFAGQVNGTTGYEEAGGQGLIAGINAHINCHGGEAFT
LARDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILLRMDDADMRLTERAYHLGLAREDRYQLMKTKKEALEQIVNFAKN
YSMKPALINDALEKLGTTPLRQGCKLIEILNRPQITIENIAEHVPAFQRELEKATAADSDRKEEILEAAEILIKYQGYID
RERMIAEKLARLESIKIKGKFDYASIQSLSTEARQKLVKIDPETIAQASRIPGVSPSDINVLLVLSGR
>Mature_628_residues
MDFKYDVIVIGAGHAGCEAAAAAANLGSKTCLITMDMNKIGQMSCNPAVGGIAKGQIVREIDALGGQMGLVTDETAIQFR
ILNRSKGPAMWSPRAQCDRAKFIWSWREKLENTPNLHIWQDTVCELLVENGEVVGLVTLWGVTFKAKCIVLTAGTFLNGL
MHVGRHQLPGGRMAEPASYQLTESIARHGIAYGRMKTGTPVRIDARSIHFDLMDTQDGECDFHKFSFMNTSTRHLKQLQC
WTCYTNEEVHRILREGLPDSPLFNGQIQSIGPRYCPSIETKIVTFPDKEQHQLFLEPEGETTQELYLNGFSSSLPMDIQI
AALKKVPAFKDIVIYRPGYAIEYDYFDPTQLKHSLESKIIKNLFFAGQVNGTTGYEEAGGQGLIAGINAHINCHGGEAFT
LARDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILLRMDDADMRLTERAYHLGLAREDRYQLMKTKKEALEQIVNFAKN
YSMKPALINDALEKLGTTPLRQGCKLIEILNRPQITIENIAEHVPAFQRELEKATAADSDRKEEILEAAEILIKYQGYID
RERMIAEKLARLESIKIKGKFDYASIQSLSTEARQKLVKIDPETIAQASRIPGVSPSDINVLLVLSGR

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=639, Percent_Identity=38.6541471048513, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI19882217, Length=664, Percent_Identity=37.8012048192771, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI183227703, Length=679, Percent_Identity=36.3770250368188, Blast_Score=402, Evalue=1e-112,
Organism=Escherichia coli, GI2367273, Length=625, Percent_Identity=47.68, Blast_Score=572, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI17534255, Length=624, Percent_Identity=38.1410256410256, Blast_Score=431, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6321202, Length=625, Percent_Identity=40, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24658174, Length=632, Percent_Identity=40.6645569620253, Blast_Score=423, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_BACTN (Q8A2N7)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_812180.1
- ProteinModelPortal:   Q8A2N7
- SMR:   Q8A2N7
- GeneID:   1072307
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_3268
- NMPDR:   fig|226186.1.peg.3267
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- PhylomeDB:   Q8A2N7
- ProtClustDB:   PRK05192
- BioCyc:   BTHE226186:BT_3268-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 70258; Mature: 70258

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 123-123 BINDING 178-178 BINDING 368-368

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKYDVIVIGAGHAGCEAAAAAANLGSKTCLITMDMNKIGQMSCNPAVGGIAKGQIVRE
CCCEEEEEEEECCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
IDALGGQMGLVTDETAIQFRILNRSKGPAMWSPRAQCDRAKFIWSWREKLENTPNLHIWQ
HHHCCCCCCEEECCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEH
DTVCELLVENGEVVGLVTLWGVTFKAKCIVLTAGTFLNGLMHVGRHQLPGGRMAEPASYQ
HHHHHHHHCCCCEEEEEEEECCEEEEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCHH
LTESIARHGIAYGRMKTGTPVRIDARSIHFDLMDTQDGECDFHKFSFMNTSTRHLKQLQC
HHHHHHHCCCEECCCCCCCCEEEECCEEEEEEECCCCCCCCCEEEEECCCCHHHHHHEEE
WTCYTNEEVHRILREGLPDSPLFNGQIQSIGPRYCPSIETKIVTFPDKEQHQLFLEPEGE
EEECCCHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCEEEEECCCCCCCEEEECCCCC
TTQELYLNGFSSSLPMDIQIAALKKVPAFKDIVIYRPGYAIEYDYFDPTQLKHSLESKII
CCHHHHEECCCCCCCCEEEEEEHHCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHH
KNLFFAGQVNGTTGYEEAGGQGLIAGINAHINCHGGEAFTLARDEAYIGVLIDDLVTKGV
HHHHHCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCEEEEECCCCEEEHHHHHHHHCCC
DEPYRMFTSRAEYRILLRMDDADMRLTERAYHLGLAREDRYQLMKTKKEALEQIVNFAKN
CHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
YSMKPALINDALEKLGTTPLRQGCKLIEILNRPQITIENIAEHVPAFQRELEKATAADSD
CCCCHHHHHHHHHHHCCCHHHHCCHHHHHHCCCCEEHHHHHHHCHHHHHHHHHHHCCCCH
RKEEILEAAEILIKYQGYIDRERMIAEKLARLESIKIKGKFDYASIQSLSTEARQKLVKI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHEEC
DPETIAQASRIPGVSPSDINVLLVLSGR
CHHHHHHHHHCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MDFKYDVIVIGAGHAGCEAAAAAANLGSKTCLITMDMNKIGQMSCNPAVGGIAKGQIVRE
CCCEEEEEEEECCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
IDALGGQMGLVTDETAIQFRILNRSKGPAMWSPRAQCDRAKFIWSWREKLENTPNLHIWQ
HHHCCCCCCEEECCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEH
DTVCELLVENGEVVGLVTLWGVTFKAKCIVLTAGTFLNGLMHVGRHQLPGGRMAEPASYQ
HHHHHHHHCCCCEEEEEEEECCEEEEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCHH
LTESIARHGIAYGRMKTGTPVRIDARSIHFDLMDTQDGECDFHKFSFMNTSTRHLKQLQC
HHHHHHHCCCEECCCCCCCCEEEECCEEEEEEECCCCCCCCCEEEEECCCCHHHHHHEEE
WTCYTNEEVHRILREGLPDSPLFNGQIQSIGPRYCPSIETKIVTFPDKEQHQLFLEPEGE
EEECCCHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCEEEEECCCCCCCEEEECCCCC
TTQELYLNGFSSSLPMDIQIAALKKVPAFKDIVIYRPGYAIEYDYFDPTQLKHSLESKII
CCHHHHEECCCCCCCCEEEEEEHHCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHH
KNLFFAGQVNGTTGYEEAGGQGLIAGINAHINCHGGEAFTLARDEAYIGVLIDDLVTKGV
HHHHHCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCEEEEECCCCEEEHHHHHHHHCCC
DEPYRMFTSRAEYRILLRMDDADMRLTERAYHLGLAREDRYQLMKTKKEALEQIVNFAKN
CHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
YSMKPALINDALEKLGTTPLRQGCKLIEILNRPQITIENIAEHVPAFQRELEKATAADSD
CCCCHHHHHHHHHHHCCCHHHHCCHHHHHHCCCCEEHHHHHHHCHHHHHHHHHHHCCCCH
RKEEILEAAEILIKYQGYIDRERMIAEKLARLESIKIKGKFDYASIQSLSTEARQKLVKI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHEEC
DPETIAQASRIPGVSPSDINVLLVLSGR
CHHHHHHHHHCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928