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The map label for this gene is sacC [H]

Identifier: 29348491

GI number: 29348491

Start: 3906644

End: 3908290

Strand: Reverse

Name: sacC [H]

Synonym: BT_3082

Alternate gene names: 29348491

Gene position: 3908290-3906644 (Counterclockwise)

Preceding gene: 29348492

Following gene: 29348490

Centisome position: 62.43

GC content: 48.76

Gene sequence:

>1647_bases
ATGAAGACATTAAGCAGAACTGTACTTACTCTTTTTGTTTTATCCTTCTCCTGTTTGGCAGCGATAGCCGGAGAAGTATC
TTTTAAAATAACCAAGCAATATCTGAACTTCCCCATCTCCCATAAAGAGAACAGAGGAAGGATGACTTTTGAAGTAAACG
GCAAGCCGGATTTATCCGTCGTTATCCGTCTGGCGCCGGATGAAGCGGAATACTGGGTGTTTAAAGATGTGTCGGCTTTC
AAAGGAAAGACGATCAAGATAACCTACGACGGCAATGAGAAGGGATTGAGTAAAATATACCAGTCCGACGAAATCGAAGG
ACAAGCTGAACTGTATAAGGAAAAGAACCGTCCGCAATTCCACTTCACTACCCGGAGAGGCTGGATCAATGACCCTAACG
GATTAATCTATTATGAGGGCGAATACCACCTGTTCTACCAGCACAATCCTTTCGAAAGAGACTGGGAGAATATGCACTGG
GGCCATGCGGTCAGCAAGGACTTGATACACTGGACAGAATTACCGGACGCTCTGTATCCCGACCATTTAGGAACGATGTT
CTCCGGTTCGGCAGTCATCGACTATGATAATACCGCCGGATTCAATAAAGGCAAGACTCCAGCCATGGTTGCCGCCTTCA
CTGCCGCCAGCAGTGACAGACAAGTGCAGGGGATAGCCTATAGCCTTGACAAAGGACGTACCTTCACGAAGTACGACAAG
AATCCGGTGATCAACTCCAAGGAGAAATGGAACTCTCAGGACACACGCGACCCGAAAGTGTTCTGGTATGCGCCCTCCAA
ACATTGGGTACTGGTACTGAACGAGCGCGACGGACACTCCATTTATACCTCTTCCAATCTCAAAGACTGGAAATACGAGA
GCCATGTTACAGGCTTTTGGGAATGTCCCGAATTGTTTGAACTGCCGGTTGACGGTGACAAGAACCATACGAAGTGGGTG
ATGTACGGTGCCACCGGTACGTATATGCTAGGCTCTTTCGACGGCAAGGTATTTACTCCCGAAGCCGGAAAATACTGCTA
TACCACCGGAAGTATCTATGCAGCCCAGACCTTTACCAATATTCCGGCAAGTGACGGCAGACGTATCCAGATAGGCTGGG
GAAGAATCTCCCACCCCGGTATGCCTTTCAACGGAATGATGATGCTCCCCACCGAACTGACTTTGCGTACCACCAAAGAC
GGCATCCGCCTTGTCAACGTTCCCGTAAAGGAGGTCGAATCCTTGTGTAAGCCTCTTCGTTCCTGGAAATCTCTTTCTTC
GGATGAGGCCAACCGCCATCTGAAAGAGTTTTATGATGCCGACTGTCTCCGCATTAAGACAACGATCAAACTCTCTCATG
CCACGGATGCCGGATTCAACCTTTTCGGCCAGCGCATGATCGGCTACGACATGAACTCCAATACCCTGAACGGCCGTTTC
TACTCTCCCCAAGACCCGACCAGCATGGAGTTGAGTGCCGATATTTACATTGACCGCACCTCTATCGAAGTATTTATCGA
CGGCGGACTGTATTCTTATTCCATGGAACGCCGCCCTGACACTAACAACAGAGAAGGACTCCACTTCTGGGGAAACCGTA
TCGAAGTGAAAGACCTGCAAGTCTTTTCCGTAGAATCCATTTGGTAG

Upstream 100 bases:

>100_bases
ATGTAACGTTTCTCATAGTCTGTGAATCATTTATTATATCAGAAATCGGTCGTATTGGCTACATTTACTCCGATTTTACA
AACTAATAAATAAAATAGCT

Downstream 100 bases:

>100_bases
ACGGAAATCAAAAGCTATCTTTGTTTTCAGTTATTTAAATAGGAAAGAGAATATGAAAACGCGTAGTGAAGTTTTTCAGT
TTGAGAAAGAGTTGAAATGG

Product: 2,6-beta-D-fructofuranosidase

Products: NA

Alternate protein names: Beta-D-fructofuranosidase; Exo-beta-D-fructosidase; Exo-levanase [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MKTLSRTVLTLFVLSFSCLAAIAGEVSFKITKQYLNFPISHKENRGRMTFEVNGKPDLSVVIRLAPDEAEYWVFKDVSAF
KGKTIKITYDGNEKGLSKIYQSDEIEGQAELYKEKNRPQFHFTTRRGWINDPNGLIYYEGEYHLFYQHNPFERDWENMHW
GHAVSKDLIHWTELPDALYPDHLGTMFSGSAVIDYDNTAGFNKGKTPAMVAAFTAASSDRQVQGIAYSLDKGRTFTKYDK
NPVINSKEKWNSQDTRDPKVFWYAPSKHWVLVLNERDGHSIYTSSNLKDWKYESHVTGFWECPELFELPVDGDKNHTKWV
MYGATGTYMLGSFDGKVFTPEAGKYCYTTGSIYAAQTFTNIPASDGRRIQIGWGRISHPGMPFNGMMMLPTELTLRTTKD
GIRLVNVPVKEVESLCKPLRSWKSLSSDEANRHLKEFYDADCLRIKTTIKLSHATDAGFNLFGQRMIGYDMNSNTLNGRF
YSPQDPTSMELSADIYIDRTSIEVFIDGGLYSYSMERRPDTNNREGLHFWGNRIEVKDLQVFSVESIW

Sequences:

>Translated_548_residues
MKTLSRTVLTLFVLSFSCLAAIAGEVSFKITKQYLNFPISHKENRGRMTFEVNGKPDLSVVIRLAPDEAEYWVFKDVSAF
KGKTIKITYDGNEKGLSKIYQSDEIEGQAELYKEKNRPQFHFTTRRGWINDPNGLIYYEGEYHLFYQHNPFERDWENMHW
GHAVSKDLIHWTELPDALYPDHLGTMFSGSAVIDYDNTAGFNKGKTPAMVAAFTAASSDRQVQGIAYSLDKGRTFTKYDK
NPVINSKEKWNSQDTRDPKVFWYAPSKHWVLVLNERDGHSIYTSSNLKDWKYESHVTGFWECPELFELPVDGDKNHTKWV
MYGATGTYMLGSFDGKVFTPEAGKYCYTTGSIYAAQTFTNIPASDGRRIQIGWGRISHPGMPFNGMMMLPTELTLRTTKD
GIRLVNVPVKEVESLCKPLRSWKSLSSDEANRHLKEFYDADCLRIKTTIKLSHATDAGFNLFGQRMIGYDMNSNTLNGRF
YSPQDPTSMELSADIYIDRTSIEVFIDGGLYSYSMERRPDTNNREGLHFWGNRIEVKDLQVFSVESIW
>Mature_548_residues
MKTLSRTVLTLFVLSFSCLAAIAGEVSFKITKQYLNFPISHKENRGRMTFEVNGKPDLSVVIRLAPDEAEYWVFKDVSAF
KGKTIKITYDGNEKGLSKIYQSDEIEGQAELYKEKNRPQFHFTTRRGWINDPNGLIYYEGEYHLFYQHNPFERDWENMHW
GHAVSKDLIHWTELPDALYPDHLGTMFSGSAVIDYDNTAGFNKGKTPAMVAAFTAASSDRQVQGIAYSLDKGRTFTKYDK
NPVINSKEKWNSQDTRDPKVFWYAPSKHWVLVLNERDGHSIYTSSNLKDWKYESHVTGFWECPELFELPVDGDKNHTKWV
MYGATGTYMLGSFDGKVFTPEAGKYCYTTGSIYAAQTFTNIPASDGRRIQIGWGRISHPGMPFNGMMMLPTELTLRTTKD
GIRLVNVPVKEVESLCKPLRSWKSLSSDEANRHLKEFYDADCLRIKTTIKLSHATDAGFNLFGQRMIGYDMNSNTLNGRF
YSPQDPTSMELSADIYIDRTSIEVFIDGGLYSYSMERRPDTNNREGLHFWGNRIEVKDLQVFSVESIW

Specific function: Exo-fructosidase that can hydrolyze both levan and inulin, leading to the production of free fructose. Is also able to hydrolyze sucrose and to a small extent raffinose, but not melezitose, stachylose, cellobiose, maltose, and lactose [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=314, Percent_Identity=37.8980891719745, Blast_Score=184, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR010496
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR011040 [H]

Pfam domain/function: PF06439 DUF1080; PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.80 [H]

Molecular weight: Translated: 62871; Mature: 62871

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLSRTVLTLFVLSFSCLAAIAGEVSFKITKQYLNFPISHKENRGRMTFEVNGKPDLSV
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCEEEEEECCCCCEEE
VIRLAPDEAEYWVFKDVSAFKGKTIKITYDGNEKGLSKIYQSDEIEGQAELYKEKNRPQF
EEEECCCCCCEEEEECHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHCCCCEE
HFTTRRGWINDPNGLIYYEGEYHLFYQHNPFERDWENMHWGHAVSKDLIHWTELPDALYP
EEEECCCCCCCCCCEEEECCEEEEEEECCCCCCCHHCCCCCHHHHHHHHHHHCCCCCCCC
DHLGTMFSGSAVIDYDNTAGFNKGKTPAMVAAFTAASSDRQVQGIAYSLDKGRTFTKYDK
CCCCHHCCCCEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCEEEECCC
NPVINSKEKWNSQDTRDPKVFWYAPSKHWVLVLNERDGHSIYTSSNLKDWKYESHVTGFW
CCCCCCHHHCCCCCCCCCEEEEECCCCCEEEEEECCCCCEEEECCCCCCCEECCCCCCEE
ECPELFELPVDGDKNHTKWVMYGATGTYMLGSFDGKVFTPEAGKYCYTTGSIYAAQTFTN
CCCHHEECCCCCCCCCEEEEEECCCCCEEEECCCCEEECCCCCCEEEECCCEEEEEEECC
IPASDGRRIQIGWGRISHPGMPFNGMMMLPTELTLRTTKDGIRLVNVPVKEVESLCKPLR
CCCCCCCEEEEECCCCCCCCCCCCCEEEECCEEEEEECCCCEEEEECCHHHHHHHHHHHH
SWKSLSSDEANRHLKEFYDADCLRIKTTIKLSHATDAGFNLFGQRMIGYDMNSNTLNGRF
HHHCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHCCEEEEEECCCCEECCEE
YSPQDPTSMELSADIYIDRTSIEVFIDGGLYSYSMERRPDTNNREGLHFWGNRIEVKDLQ
ECCCCCCCEEEEEEEEEEEEEEEEEECCCEEEEEECCCCCCCCCCCEEECCCEEEEEEEE
VFSVESIW
EEEEECCC
>Mature Secondary Structure
MKTLSRTVLTLFVLSFSCLAAIAGEVSFKITKQYLNFPISHKENRGRMTFEVNGKPDLSV
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCEEEEEECCCCCEEE
VIRLAPDEAEYWVFKDVSAFKGKTIKITYDGNEKGLSKIYQSDEIEGQAELYKEKNRPQF
EEEECCCCCCEEEEECHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHCCCCEE
HFTTRRGWINDPNGLIYYEGEYHLFYQHNPFERDWENMHWGHAVSKDLIHWTELPDALYP
EEEECCCCCCCCCCEEEECCEEEEEEECCCCCCCHHCCCCCHHHHHHHHHHHCCCCCCCC
DHLGTMFSGSAVIDYDNTAGFNKGKTPAMVAAFTAASSDRQVQGIAYSLDKGRTFTKYDK
CCCCHHCCCCEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCEEEECCC
NPVINSKEKWNSQDTRDPKVFWYAPSKHWVLVLNERDGHSIYTSSNLKDWKYESHVTGFW
CCCCCCHHHCCCCCCCCCEEEEECCCCCEEEEEECCCCCEEEECCCCCCCEECCCCCCEE
ECPELFELPVDGDKNHTKWVMYGATGTYMLGSFDGKVFTPEAGKYCYTTGSIYAAQTFTN
CCCHHEECCCCCCCCCEEEEEECCCCCEEEECCCCEEECCCCCCEEEECCCEEEEEEECC
IPASDGRRIQIGWGRISHPGMPFNGMMMLPTELTLRTTKDGIRLVNVPVKEVESLCKPLR
CCCCCCCEEEEECCCCCCCCCCCCCEEEECCEEEEEECCCCEEEEECCHHHHHHHHHHHH
SWKSLSSDEANRHLKEFYDADCLRIKTTIKLSHATDAGFNLFGQRMIGYDMNSNTLNGRF
HHHCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHCCEEEEEECCCCEECCEE
YSPQDPTSMELSADIYIDRTSIEVFIDGGLYSYSMERRPDTNNREGLHFWGNRIEVKDLQ
ECCCCCCCEEEEEEEEEEEEEEEEEECCCEEEEEECCCCCCCCCCCEEECCCEEEEEEEE
VFSVESIW
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3112519; 3120151; 9141695; 9384377; 2117666 [H]