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The map label for this gene is ribBA

Identifier: 29347826

GI number: 29347826

Start: 3016902

End: 3018116

Strand: Reverse

Name: ribBA

Synonym: BT_2416

Alternate gene names: 29347826

Gene position: 3018116-3016902 (Counterclockwise)

Preceding gene: 29347827

Following gene: 29347825

Centisome position: 48.21

GC content: 44.77

Gene sequence:

>1215_bases
ATGGAAGAGATTAAAATGGATAGAATAGAAGATGCGATTGCCGATTTCAAAGAAGGCAAGTTTGTCATTGTAGTGGATGA
TGAAGATCGTGAAAACGAAGGTGATTTAATCATAGCTGCCGAAAAGATAACTCCTGAGAAAGTTAACTTTATGTTGAAAC
ATGCGCGTGGTGTGCTCTGTGCGCCTATCACGGTGTCGCGTTGTAAAGAACTGGATTTGCCGCATCAGGTCAGTGATAAT
ACTTCTGTATTGGGCACTCCTTTTACAGTGACAATTGACAAACTGGAAGGTTGTAGTACCGGAGTTTCTGCTTCCGACCG
TGCTGCCACTATTCAGGCGTTGGCCGATCCTGCTTCTACACCGGCTACTTTCGGACGTCCCGGACATATTAATCCGCTGT
ATGCACAGGAAAAAGGAGTACTTCGTCGTGCCGGACATACAGAAGCTACCATCGATATGGCACGCCTTGCCGGACTTTAC
CCTGCAGGTGCTCTGATGGAAATAATGAGCGATGACGGTACGATGGCACGTCTGCCGGAACTTCGTGCAATGGCCGATGA
ACATGGACTGAAACTGATTTCCATTCATGACTTGATTGCTTATCGTCTGAAACAGGAGTCAATCGTAGAAAAAGGCGTTG
AGGTAAATATGCCGACCGAACATGGTATGTTCCGTCTGATTCCTTTCCGTCAGAAATCGAATGGACTGGAGCATATGGCT
ATATTTAAAGGTACATGGAATGAAGACGAACCTATCTTGGTGCGTGTCCATTCATCTTGCGCTACTGGTGATATTCTTGG
CTCACACCGTTGTGACTGTGGCGAGCAACTGCATAAAGCGATGGAAATGATTGAAAAAGAAGGAAAAGGTGTGGTGGTTT
ATCTGAATCAGGAAGGCCGTGGTATCGGTTTGATGGAAAAGATGAAAGCATATAAACTTCAGGAAGACGGTATGGATACC
GTTGACGCCAACCTTTGCTTAGGACACCTGGCAGACGAACGCGACTATGGAGTAGGAGCCCAGATTCTGCGTGAACTGGG
AGTGCACAAAATGAAACTTCTGACAAATAATCCCGTGAAACGTGTAGGGCTGGAAGCCTATGGACTGGAAATTGTAGAAA
ATGTACCTGTTGAAACAGTTCCTAATCCATATAATGAGCGATATCTGAGAACAAAGAAAGAAAGAATGGGACATACATTA
CATTTTAATAAGTAA

Upstream 100 bases:

>100_bases
TCTGGGCATTCCGTGTCCGGTTGGCGAAAGATATGGAACGGATAATAAAAAACAATGAACAAATTCAATTCATCATACAG
AATATTAATAAATAAGTGTT

Downstream 100 bases:

>100_bases
AATGCCATTTTGTTTTCATATATCGAATATTATCGCTTATTTTGCAACGAATTTCACTCAACAACAATAAAATAGATACA
AAATGAATCAATTATCAGAT

Product: GTP cyclohydrolase II

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MEEIKMDRIEDAIADFKEGKFVIVVDDEDRENEGDLIIAAEKITPEKVNFMLKHARGVLCAPITVSRCKELDLPHQVSDN
TSVLGTPFTVTIDKLEGCSTGVSASDRAATIQALADPASTPATFGRPGHINPLYAQEKGVLRRAGHTEATIDMARLAGLY
PAGALMEIMSDDGTMARLPELRAMADEHGLKLISIHDLIAYRLKQESIVEKGVEVNMPTEHGMFRLIPFRQKSNGLEHMA
IFKGTWNEDEPILVRVHSSCATGDILGSHRCDCGEQLHKAMEMIEKEGKGVVVYLNQEGRGIGLMEKMKAYKLQEDGMDT
VDANLCLGHLADERDYGVGAQILRELGVHKMKLLTNNPVKRVGLEAYGLEIVENVPVETVPNPYNERYLRTKKERMGHTL
HFNK

Sequences:

>Translated_404_residues
MEEIKMDRIEDAIADFKEGKFVIVVDDEDRENEGDLIIAAEKITPEKVNFMLKHARGVLCAPITVSRCKELDLPHQVSDN
TSVLGTPFTVTIDKLEGCSTGVSASDRAATIQALADPASTPATFGRPGHINPLYAQEKGVLRRAGHTEATIDMARLAGLY
PAGALMEIMSDDGTMARLPELRAMADEHGLKLISIHDLIAYRLKQESIVEKGVEVNMPTEHGMFRLIPFRQKSNGLEHMA
IFKGTWNEDEPILVRVHSSCATGDILGSHRCDCGEQLHKAMEMIEKEGKGVVVYLNQEGRGIGLMEKMKAYKLQEDGMDT
VDANLCLGHLADERDYGVGAQILRELGVHKMKLLTNNPVKRVGLEAYGLEIVENVPVETVPNPYNERYLRTKKERMGHTL
HFNK
>Mature_404_residues
MEEIKMDRIEDAIADFKEGKFVIVVDDEDRENEGDLIIAAEKITPEKVNFMLKHARGVLCAPITVSRCKELDLPHQVSDN
TSVLGTPFTVTIDKLEGCSTGVSASDRAATIQALADPASTPATFGRPGHINPLYAQEKGVLRRAGHTEATIDMARLAGLY
PAGALMEIMSDDGTMARLPELRAMADEHGLKLISIHDLIAYRLKQESIVEKGVEVNMPTEHGMFRLIPFRQKSNGLEHMA
IFKGTWNEDEPILVRVHSSCATGDILGSHRCDCGEQLHKAMEMIEKEGKGVVVYLNQEGRGIGLMEKMKAYKLQEDGMDT
VDANLCLGHLADERDYGVGAQILRELGVHKMKLLTNNPVKRVGLEAYGLEIVENVPVETVPNPYNERYLRTKKERMGHTL
HFNK

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate

COG id: COG0807

COG function: function code H; GTP cyclohydrolase II

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family

Homologues:

Organism=Escherichia coli, GI1789420, Length=201, Percent_Identity=51.7412935323383, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1787533, Length=193, Percent_Identity=47.1502590673575, Blast_Score=180, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6320695, Length=201, Percent_Identity=47.2636815920398, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6319438, Length=186, Percent_Identity=46.7741935483871, Blast_Score=145, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIBBA_BACTN (Q8A528)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_811329.1
- HSSP:   P0A7J0
- ProteinModelPortal:   Q8A528
- GeneID:   1073662
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_2416
- NMPDR:   fig|226186.1.peg.2416
- HOGENOM:   HBG735778
- OMA:   NIVAREP
- PhylomeDB:   Q8A528
- ProtClustDB:   CLSK823142
- BioCyc:   BTHE226186:BT_2416-MONOMER
- HAMAP:   MF_01283
- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299
- Gene3D:   G3DSA:3.90.870.10
- PIRSF:   PIRSF001259
- TIGRFAMs:   TIGR00505
- TIGRFAMs:   TIGR00506

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2; SSF55821 DHBP_synth_RibB-like_a/b_dom

EC number: =4.1.99.12; =3.5.4.25

Molecular weight: Translated: 44824; Mature: 44824

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: ACT_SITE 332-332 ACT_SITE 334-334 BINDING 35-35 BINDING 167-167 BINDING 276-276 BINDING 320-320 BINDING 355-355 BINDING 360-360

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEIKMDRIEDAIADFKEGKFVIVVDDEDRENEGDLIIAAEKITPEKVNFMLKHARGVLC
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEE
APITVSRCKELDLPHQVSDNTSVLGTPFTVTIDKLEGCSTGVSASDRAATIQALADPAST
CCCCHHHHHHCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCC
PATFGRPGHINPLYAQEKGVLRRAGHTEATIDMARLAGLYPAGALMEIMSDDGTMARLPE
CCCCCCCCCCCCCHHHHCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHCHH
LRAMADEHGLKLISIHDLIAYRLKQESIVEKGVEVNMPTEHGMFRLIPFRQKSNGLEHMA
HHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEEECCCCCCCCCEEE
IFKGTWNEDEPILVRVHSSCATGDILGSHRCDCGEQLHKAMEMIEKEGKGVVVYLNQEGR
EEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCC
GIGLMEKMKAYKLQEDGMDTVDANLCLGHLADERDYGVGAQILRELGVHKMKLLTNNPVK
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEHHCCCCHH
RVGLEAYGLEIVENVPVETVPNPYNERYLRTKKERMGHTLHFNK
HHCHHHHCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MEEIKMDRIEDAIADFKEGKFVIVVDDEDRENEGDLIIAAEKITPEKVNFMLKHARGVLC
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEE
APITVSRCKELDLPHQVSDNTSVLGTPFTVTIDKLEGCSTGVSASDRAATIQALADPAST
CCCCHHHHHHCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCC
PATFGRPGHINPLYAQEKGVLRRAGHTEATIDMARLAGLYPAGALMEIMSDDGTMARLPE
CCCCCCCCCCCCCHHHHCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHCHH
LRAMADEHGLKLISIHDLIAYRLKQESIVEKGVEVNMPTEHGMFRLIPFRQKSNGLEHMA
HHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEEECCCCCCCCCEEE
IFKGTWNEDEPILVRVHSSCATGDILGSHRCDCGEQLHKAMEMIEKEGKGVVVYLNQEGR
EEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCC
GIGLMEKMKAYKLQEDGMDTVDANLCLGHLADERDYGVGAQILRELGVHKMKLLTNNPVK
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEHHCCCCHH
RVGLEAYGLEIVENVPVETVPNPYNERYLRTKKERMGHTLHFNK
HHCHHHHCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12663928