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The map label for this gene is mepA [H]

Identifier: 29347787

GI number: 29347787

Start: 2963646

End: 2965019

Strand: Reverse

Name: mepA [H]

Synonym: BT_2377

Alternate gene names: 29347787

Gene position: 2965019-2963646 (Counterclockwise)

Preceding gene: 29347788

Following gene: 29347784

Centisome position: 47.36

GC content: 45.85

Gene sequence:

>1374_bases
ATGAACAATAAATACGAAGAACGGCTGGGAACAGAACGTATGTTACCGCTTGTTTTTAAGATGGCACTCCCGGCAGTGGC
TGCACAATTTGTCAATCTGCTTTATAGCATTGTGGACCGTATATACATCGGGCATATTCCGGGAATTGGCACCGATGCGC
TGGCGGGTGTAGGGGTGACTACCTCTCTCATTATACTGATTTCTTCTTTTTCGGCAATTGTCGGCGGCGGTGGTGCTCCG
TTGGCTGCCATTGCACTCGGACAGGGCGACCGTGTACGAGCCGGAAAGATATTGGGAAACGGGTTTGTTCTGCTGATATT
GTTTACTTTGCTGACTTCTGTCATTGCATATACTTTTATGGAACCTATTCTCCTCTTTACAGGAGCTTCGGAAAACACCT
TGGAATATGCGGTTGACTATCTTTCCATTTATCTCTTGGGAACGATATTTGTCGAAATATCCACCGGGTTGAATAGCTTT
ATCAATGCTCAGGGACGTCCTGCCATTGCCATGTTTTCAGTTCTGATCGGAGCCTTATTGAACATCATTCTTGATCCTAT
TTTTATTTTCTGGTTTGACATGGGCGTGAAAGGTGCTGCTTTGGCTACAGTACTTTCCCAAGCATGCAGTGCGGTGTGGG
TGGTAAGTTTCCTCTTTTCCCGACGTGCCTCTCTGCCTTTGGAGAAACGATATATGGGACTGGACCGGAAGATTATCCTT
TCCATATTAGCTTTAGGAGTATCCCCCTTCATTATGGCAAGTACGGAAAGTCTGGTTGGGTTTGTCCTCAATAGCAGTCT
GAAGGAATTTGGAGATATCTACATCAGCGCATTGACGATTCTGCAAAGTTCGATGCAGTTTGCCAGTGTACCGCTGACCG
GATTTGCGCAAGGATTCGTTCCGATTATAAGTTATAACTTCGGGCATGGTGACAAGCAGCGTGTGAAAGATTGCTTCCGT
ATTGTGCTGGTAACCATGTTTTCCTTCAATTTGATATTGATGCTTTTCATGATTCTCTTCCCTTCGACGGTTGCCTCTGC
TTTCACCAGTGATGAAAGACTGATAGAAACTGTACGTTGGACAATGCCCGTTTTCTTGGGTGGCATGACCATTTTCGGCT
TGCAACGTGCCTGTCAGAATATGTTCGTTGCATTGGGGCAGGCCCGAATCTCTATCTTCATTGCTCTGTTGCGTAAAGCT
ATCCTGTTGATTCCGCTGGCGTTAATTTTGCCGAACTTTATGGGAGTAACCGGAGTGTATGCAGCTGAGGCTATATCTGA
TGCGACTGCGGCCATCTGTTGCACGTTATTGTTCTTTGGGCAGTTCCCTAAAATTCTGGGAAGGATTAAAGGGAATACTT
TGCGTGTGGAGTAA

Upstream 100 bases:

>100_bases
GGTCAGAAGGAAAGATTATACTTCAATTTTTATCACAATATAGATAAAAATTGAAGTTTTTTATCTATGTTGCAGCCGAT
TTTAGAAAAAGAAGTTTGGA

Downstream 100 bases:

>100_bases
TCGTTTCTTTGTATTTTATTTTTTTTACTTCACAGATATGCATGGCATTACGGTTGTATTCTGACTGTTATCTCTCTCTT
TATCTCATTTCCTGTGCGTA

Product: cation efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MNNKYEERLGTERMLPLVFKMALPAVAAQFVNLLYSIVDRIYIGHIPGIGTDALAGVGVTTSLIILISSFSAIVGGGGAP
LAAIALGQGDRVRAGKILGNGFVLLILFTLLTSVIAYTFMEPILLFTGASENTLEYAVDYLSIYLLGTIFVEISTGLNSF
INAQGRPAIAMFSVLIGALLNIILDPIFIFWFDMGVKGAALATVLSQACSAVWVVSFLFSRRASLPLEKRYMGLDRKIIL
SILALGVSPFIMASTESLVGFVLNSSLKEFGDIYISALTILQSSMQFASVPLTGFAQGFVPIISYNFGHGDKQRVKDCFR
IVLVTMFSFNLILMLFMILFPSTVASAFTSDERLIETVRWTMPVFLGGMTIFGLQRACQNMFVALGQARISIFIALLRKA
ILLIPLALILPNFMGVTGVYAAEAISDATAAICCTLLFFGQFPKILGRIKGNTLRVE

Sequences:

>Translated_457_residues
MNNKYEERLGTERMLPLVFKMALPAVAAQFVNLLYSIVDRIYIGHIPGIGTDALAGVGVTTSLIILISSFSAIVGGGGAP
LAAIALGQGDRVRAGKILGNGFVLLILFTLLTSVIAYTFMEPILLFTGASENTLEYAVDYLSIYLLGTIFVEISTGLNSF
INAQGRPAIAMFSVLIGALLNIILDPIFIFWFDMGVKGAALATVLSQACSAVWVVSFLFSRRASLPLEKRYMGLDRKIIL
SILALGVSPFIMASTESLVGFVLNSSLKEFGDIYISALTILQSSMQFASVPLTGFAQGFVPIISYNFGHGDKQRVKDCFR
IVLVTMFSFNLILMLFMILFPSTVASAFTSDERLIETVRWTMPVFLGGMTIFGLQRACQNMFVALGQARISIFIALLRKA
ILLIPLALILPNFMGVTGVYAAEAISDATAAICCTLLFFGQFPKILGRIKGNTLRVE
>Mature_457_residues
MNNKYEERLGTERMLPLVFKMALPAVAAQFVNLLYSIVDRIYIGHIPGIGTDALAGVGVTTSLIILISSFSAIVGGGGAP
LAAIALGQGDRVRAGKILGNGFVLLILFTLLTSVIAYTFMEPILLFTGASENTLEYAVDYLSIYLLGTIFVEISTGLNSF
INAQGRPAIAMFSVLIGALLNIILDPIFIFWFDMGVKGAALATVLSQACSAVWVVSFLFSRRASLPLEKRYMGLDRKIIL
SILALGVSPFIMASTESLVGFVLNSSLKEFGDIYISALTILQSSMQFASVPLTGFAQGFVPIISYNFGHGDKQRVKDCFR
IVLVTMFSFNLILMLFMILFPSTVASAFTSDERLIETVRWTMPVFLGGMTIFGLQRACQNMFVALGQARISIFIALLRKA
ILLIPLALILPNFMGVTGVYAAEAISDATAAICCTLLFFGQFPKILGRIKGNTLRVE

Specific function: Multidrug resistance efflux protein [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MepA subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510954, Length=222, Percent_Identity=28.3783783783784, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR015522 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49529; Mature: 49529

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKYEERLGTERMLPLVFKMALPAVAAQFVNLLYSIVDRIYIGHIPGIGTDALAGVGVT
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHH
TSLIILISSFSAIVGGGGAPLAAIALGQGDRVRAGKILGNGFVLLILFTLLTSVIAYTFM
HHHHHHHHHHHHHHCCCCCCHHHEEECCCCCEECCHHHCCHHHHHHHHHHHHHHHHHHHH
EPILLFTGASENTLEYAVDYLSIYLLGTIFVEISTGLNSFINAQGRPAIAMFSVLIGALL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
NIILDPIFIFWFDMGVKGAALATVLSQACSAVWVVSFLFSRRASLPLEKRYMGLDRKIIL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHH
SILALGVSPFIMASTESLVGFVLNSSLKEFGDIYISALTILQSSMQFASVPLTGFAQGFV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
PIISYNFGHGDKQRVKDCFRIVLVTMFSFNLILMLFMILFPSTVASAFTSDERLIETVRW
HHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
TMPVFLGGMTIFGLQRACQNMFVALGQARISIFIALLRKAILLIPLALILPNFMGVTGVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAEAISDATAAICCTLLFFGQFPKILGRIKGNTLRVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure
MNNKYEERLGTERMLPLVFKMALPAVAAQFVNLLYSIVDRIYIGHIPGIGTDALAGVGVT
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHH
TSLIILISSFSAIVGGGGAPLAAIALGQGDRVRAGKILGNGFVLLILFTLLTSVIAYTFM
HHHHHHHHHHHHHHCCCCCCHHHEEECCCCCEECCHHHCCHHHHHHHHHHHHHHHHHHHH
EPILLFTGASENTLEYAVDYLSIYLLGTIFVEISTGLNSFINAQGRPAIAMFSVLIGALL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
NIILDPIFIFWFDMGVKGAALATVLSQACSAVWVVSFLFSRRASLPLEKRYMGLDRKIIL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHH
SILALGVSPFIMASTESLVGFVLNSSLKEFGDIYISALTILQSSMQFASVPLTGFAQGFV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
PIISYNFGHGDKQRVKDCFRIVLVTMFSFNLILMLFMILFPSTVASAFTSDERLIETVRW
HHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
TMPVFLGGMTIFGLQRACQNMFVALGQARISIFIALLRKAILLIPLALILPNFMGVTGVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAEAISDATAAICCTLLFFGQFPKILGRIKGNTLRVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA