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The map label for this gene is mutB [H]

Identifier: 29347500

GI number: 29347500

Start: 2618135

End: 2620282

Strand: Reverse

Name: mutB [H]

Synonym: BT_2090

Alternate gene names: 29347500

Gene position: 2620282-2618135 (Counterclockwise)

Preceding gene: 29347501

Following gene: 29347499

Centisome position: 41.86

GC content: 47.95

Gene sequence:

>2148_bases
ATGAGAAAAGATTTTAAAAACTTAGATATATATGCTGCATTTCAGCCCGCTAACGGTGCTGAATGGCAAAAGGCTAACGG
TATTTCCGCTGATTGGAATACACCGGAACACATTGACGTGAAGCCTGTTTATACAAAAGAGGACCTCGAAGGAATGGAAC
ACCTCGGCTATGCTGCCGGACTTCCTCCTTATCTGCGTGGTCCGTATTCAGTGATGTACACTCTTCGCCCCTGGACTATC
CGTCAGTACGCCGGATTCTCTACTGCCGAAGAATCAAATGCTTTCTATCGCCGTAACCTGGCTTCCGGTCAGAAAGGTTT
GTCCGTAGCATTTGACTTGGCTACTCACCGCGGTTATGACCCCGATCACGAACGTGTGGTAGGTGACGTAGGTAAGGCTG
GTGTATCTATCTGTTCACTCGAAAACATGAAAGTTTTGTTCGACGGTATTCCTTTGAGCAAGATGTCCGTGTCTATGACG
ATGAACGGTGCGGTACTTCCGATTATGGCATTCTATATTAATGCAGGTCTGGAACAGGGTGCTAAACTCGAAGAAATGGC
CGGTACTATCCAGAATGATATTCTGAAAGAGTTCATGGTGCGTAATACCTATATCTATCCGCCTGCATTCTCTATGAAGA
TTATTTCCGATATTTTCGAATATACTTCGCAGAAGATGCCTAAGTTCAACTCCATCTCTATCTCCGGTTACCACATGCAG
GAAGCAGGTGCGACGGCAGATATCGAGCTGGCTTATACATTGGCCGACGGTCTGGAATATCTGCGTGCCGGAACAGCGGC
AGGTATCGATATCGATGCGTTCGCTCCCCGTCTGTCTTTCTTCTGGGCGATTGGTACCAATCACTTCATGGAAATCGCGA
AGATGCGTGCTGCCCGTATGTTGTGGGCTAAGATTGTAAAACAATTCAATCCGAAGAATCCGAAATCACTGGCATTGCGT
ACACACTCTCAGACTTCCGGCTGGTCGCTGACAGAACAAGACCCGTTCAATAACGTAGGACGTACTTGTATCGAAGCCAT
GGCTGCCGCTTTGGGACATACACAGTCTTTGCATACCAATGCGCTGGATGAGGCTATTGCCCTACCGACAGACTTCTCCG
CACGTATTGCCCGTAATACTCAGATTTATATTCAGGAAGAAACCTATATCTGTAAGAACGTTGACCCATGGGGCGGTTCT
TACTACGTAGAAGCTCTGACCAACGAACTGGCTCACAAGGCTTGGGAACGTATCGAGGAAGTAGAAAAACTGGGTGGTAT
GGCGAAAGCTATCGAGACCGGTATTCCTAAGATGCGTATCGAAGAGGCTGCTGCTCGTACGCAGGCCCGTATCGACAGCG
GTTCTCAGACTATTGTTGGTGTGAACAAATATCGTCTGGAAAAAGAAGCTCCGATTGATATCCTTGAGATTGATAACACG
GCAGTTCGTCTTGAACAGATCGAGAACCTGAAACGTTTGAAAGAAGGACGTAACCAGGCAGAAGTGGACAAAGCACTGGC
AGCCATCACCGAATGCGTGGAGACCGGTAAGGGTAACTTGCTGGAACTGGCTGTAGAAGCTGCTCGTGTTCGTGCAACGC
TGGGTGAAATCTCCTATGCTTGCGAAAAGGTTGTAGGACGTTATAAAGCAATAATTAGAACAATATCAGGCGTGTATTCA
TCAGAAAGTAAAAACGATGGCGACTTCAAGCGTGCATGTGAACTGGCTGAGAAGTTTGCTAAGAAAGAGGGACGTCAACC
TCGTATTATGGTTGCGAAGATGGGACAGGACGGTCACGACCGTGGTGCCAAAGTGGTAGCAACCGGTTATGCAGACTGTG
GCTTCGACGTAGACATGGGACCATTGTTCCAGACTCCGGCGGAAGCTGCCCGTGAAGCTGTAGAAAATGACGTTCACGTA
GTAGGTGTTTCTTCATTGGCTGCCGGACACAAGACGTTGATTCCGCAGATTATGGAAGAACTGAAAAAGCTGGGTCGTGA
AGATATCGTGGTAATTGCCGGCGGTGTTATCCCTGCACAGGATTATGACTTCCTGTATAAGGCAGGTGTAGCTGCTATTT
TCGGTCCCGGTACTCCGGTAGCGAAGGCTGCTTGCCAGATTCTGGAAATCCTGATGGATGAAGAATAA

Upstream 100 bases:

>100_bases
ATGGATGAACTGAAGGCAGCAGGTATCGAGAACTTCATCCACGTGCGTGTCAACGTATTGGATACCTTGAAAGAGTTTAA
CGCCAAACTATTGAAGTAAG

Downstream 100 bases:

>100_bases
GTACGGAATTTAATCATTTTTTCTATTTTTTTATCCCGCCTTATAGCTCCATTCGGGCTGAAGGCGGGATGTTTTTTTCA
TCTTTATGCGGTCAGTGAGA

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MRKDFKNLDIYAAFQPANGAEWQKANGISADWNTPEHIDVKPVYTKEDLEGMEHLGYAAGLPPYLRGPYSVMYTLRPWTI
RQYAGFSTAEESNAFYRRNLASGQKGLSVAFDLATHRGYDPDHERVVGDVGKAGVSICSLENMKVLFDGIPLSKMSVSMT
MNGAVLPIMAFYINAGLEQGAKLEEMAGTIQNDILKEFMVRNTYIYPPAFSMKIISDIFEYTSQKMPKFNSISISGYHMQ
EAGATADIELAYTLADGLEYLRAGTAAGIDIDAFAPRLSFFWAIGTNHFMEIAKMRAARMLWAKIVKQFNPKNPKSLALR
THSQTSGWSLTEQDPFNNVGRTCIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYIQEETYICKNVDPWGGS
YYVEALTNELAHKAWERIEEVEKLGGMAKAIETGIPKMRIEEAAARTQARIDSGSQTIVGVNKYRLEKEAPIDILEIDNT
AVRLEQIENLKRLKEGRNQAEVDKALAAITECVETGKGNLLELAVEAARVRATLGEISYACEKVVGRYKAIIRTISGVYS
SESKNDGDFKRACELAEKFAKKEGRQPRIMVAKMGQDGHDRGAKVVATGYADCGFDVDMGPLFQTPAEAAREAVENDVHV
VGVSSLAAGHKTLIPQIMEELKKLGREDIVVIAGGVIPAQDYDFLYKAGVAAIFGPGTPVAKAACQILEILMDEE

Sequences:

>Translated_715_residues
MRKDFKNLDIYAAFQPANGAEWQKANGISADWNTPEHIDVKPVYTKEDLEGMEHLGYAAGLPPYLRGPYSVMYTLRPWTI
RQYAGFSTAEESNAFYRRNLASGQKGLSVAFDLATHRGYDPDHERVVGDVGKAGVSICSLENMKVLFDGIPLSKMSVSMT
MNGAVLPIMAFYINAGLEQGAKLEEMAGTIQNDILKEFMVRNTYIYPPAFSMKIISDIFEYTSQKMPKFNSISISGYHMQ
EAGATADIELAYTLADGLEYLRAGTAAGIDIDAFAPRLSFFWAIGTNHFMEIAKMRAARMLWAKIVKQFNPKNPKSLALR
THSQTSGWSLTEQDPFNNVGRTCIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYIQEETYICKNVDPWGGS
YYVEALTNELAHKAWERIEEVEKLGGMAKAIETGIPKMRIEEAAARTQARIDSGSQTIVGVNKYRLEKEAPIDILEIDNT
AVRLEQIENLKRLKEGRNQAEVDKALAAITECVETGKGNLLELAVEAARVRATLGEISYACEKVVGRYKAIIRTISGVYS
SESKNDGDFKRACELAEKFAKKEGRQPRIMVAKMGQDGHDRGAKVVATGYADCGFDVDMGPLFQTPAEAAREAVENDVHV
VGVSSLAAGHKTLIPQIMEELKKLGREDIVVIAGGVIPAQDYDFLYKAGVAAIFGPGTPVAKAACQILEILMDEE
>Mature_715_residues
MRKDFKNLDIYAAFQPANGAEWQKANGISADWNTPEHIDVKPVYTKEDLEGMEHLGYAAGLPPYLRGPYSVMYTLRPWTI
RQYAGFSTAEESNAFYRRNLASGQKGLSVAFDLATHRGYDPDHERVVGDVGKAGVSICSLENMKVLFDGIPLSKMSVSMT
MNGAVLPIMAFYINAGLEQGAKLEEMAGTIQNDILKEFMVRNTYIYPPAFSMKIISDIFEYTSQKMPKFNSISISGYHMQ
EAGATADIELAYTLADGLEYLRAGTAAGIDIDAFAPRLSFFWAIGTNHFMEIAKMRAARMLWAKIVKQFNPKNPKSLALR
THSQTSGWSLTEQDPFNNVGRTCIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYIQEETYICKNVDPWGGS
YYVEALTNELAHKAWERIEEVEKLGGMAKAIETGIPKMRIEEAAARTQARIDSGSQTIVGVNKYRLEKEAPIDILEIDNT
AVRLEQIENLKRLKEGRNQAEVDKALAAITECVETGKGNLLELAVEAARVRATLGEISYACEKVVGRYKAIIRTISGVYS
SESKNDGDFKRACELAEKFAKKEGRQPRIMVAKMGQDGHDRGAKVVATGYADCGFDVDMGPLFQTPAEAAREAVENDVHV
VGVSSLAAGHKTLIPQIMEELKKLGREDIVVIAGGVIPAQDYDFLYKAGVAAIFGPGTPVAKAACQILEILMDEE

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=679, Percent_Identity=64.8011782032401, Blast_Score=922, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=683, Percent_Identity=62.2254758418741, Blast_Score=853, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=682, Percent_Identity=64.0762463343109, Blast_Score=899, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 78701; Mature: 78701

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00544 METMALONYL_COA_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKDFKNLDIYAAFQPANGAEWQKANGISADWNTPEHIDVKPVYTKEDLEGMEHLGYAAG
CCCCCCCCEEEEEEECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHCC
LPPYLRGPYSVMYTLRPWTIRQYAGFSTAEESNAFYRRNLASGQKGLSVAFDLATHRGYD
CCHHHCCCEEEEEEECCCEEHHHCCCCCCHHCCHHHHHHHCCCCCCCEEEEEHHHCCCCC
PDHERVVGDVGKAGVSICSLENMKVLFDGIPLSKMSVSMTMNGAVLPIMAFYINAGLEQG
CCHHHHHHHHHHHCEEEEECCCCEEEECCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCC
AKLEEMAGTIQNDILKEFMVRNTYIYPPAFSMKIISDIFEYTSQKMPKFNSISISGYHMQ
CHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEECEEEC
EAGATADIELAYTLADGLEYLRAGTAAGIDIDAFAPRLSFFWAIGTNHFMEIAKMRAARM
CCCCCCEEEEEHHHHHHHHHHHCCCCCCCCHHHHCHHHHEEEECCHHHHHHHHHHHHHHH
LWAKIVKQFNPKNPKSLALRTHSQTSGWSLTEQDPFNNVGRTCIEAMAAALGHTQSLHTN
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
ALDEAIALPTDFSARIARNTQIYIQEETYICKNVDPWGGSYYVEALTNELAHKAWERIEE
HHHHHHCCCCCCHHHHCCCCEEEEEECEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VEKLGGMAKAIETGIPKMRIEEAAARTQARIDSGSQTIVGVNKYRLEKEAPIDILEIDNT
HHHHCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHEECCCCCEEEEECCCH
AVRLEQIENLKRLKEGRNQAEVDKALAAITECVETGKGNLLELAVEAARVRATLGEISYA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
CEKVVGRYKAIIRTISGVYSSESKNDGDFKRACELAEKFAKKEGRQPRIMVAKMGQDGHD
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
RGAKVVATGYADCGFDVDMGPLFQTPAEAAREAVENDVHVVGVSSLAAGHKTLIPQIMEE
CCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEHHHHHCCHHHHHHHHHHH
LKKLGREDIVVIAGGVIPAQDYDFLYKAGVAAIFGPGTPVAKAACQILEILMDEE
HHHCCCCCEEEEECCCCCCCCCHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKDFKNLDIYAAFQPANGAEWQKANGISADWNTPEHIDVKPVYTKEDLEGMEHLGYAAG
CCCCCCCCEEEEEEECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHCC
LPPYLRGPYSVMYTLRPWTIRQYAGFSTAEESNAFYRRNLASGQKGLSVAFDLATHRGYD
CCHHHCCCEEEEEEECCCEEHHHCCCCCCHHCCHHHHHHHCCCCCCCEEEEEHHHCCCCC
PDHERVVGDVGKAGVSICSLENMKVLFDGIPLSKMSVSMTMNGAVLPIMAFYINAGLEQG
CCHHHHHHHHHHHCEEEEECCCCEEEECCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCC
AKLEEMAGTIQNDILKEFMVRNTYIYPPAFSMKIISDIFEYTSQKMPKFNSISISGYHMQ
CHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEECEEEC
EAGATADIELAYTLADGLEYLRAGTAAGIDIDAFAPRLSFFWAIGTNHFMEIAKMRAARM
CCCCCCEEEEEHHHHHHHHHHHCCCCCCCCHHHHCHHHHEEEECCHHHHHHHHHHHHHHH
LWAKIVKQFNPKNPKSLALRTHSQTSGWSLTEQDPFNNVGRTCIEAMAAALGHTQSLHTN
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
ALDEAIALPTDFSARIARNTQIYIQEETYICKNVDPWGGSYYVEALTNELAHKAWERIEE
HHHHHHCCCCCCHHHHCCCCEEEEEECEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VEKLGGMAKAIETGIPKMRIEEAAARTQARIDSGSQTIVGVNKYRLEKEAPIDILEIDNT
HHHHCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHEECCCCCEEEEECCCH
AVRLEQIENLKRLKEGRNQAEVDKALAAITECVETGKGNLLELAVEAARVRATLGEISYA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
CEKVVGRYKAIIRTISGVYSSESKNDGDFKRACELAEKFAKKEGRQPRIMVAKMGQDGHD
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
RGAKVVATGYADCGFDVDMGPLFQTPAEAAREAVENDVHVVGVSSLAAGHKTLIPQIMEE
CCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEHHHHHCCHHHHHHHHHHH
LKKLGREDIVVIAGGVIPAQDYDFLYKAGVAAIFGPGTPVAKAACQILEILMDEE
HHHCCCCCEEEEECCCCCCCCCHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8566763; 12949112 [H]