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The map label for this gene is topB [H]

Identifier: 29347499

GI number: 29347499

Start: 2615821

End: 2617983

Strand: Reverse

Name: topB [H]

Synonym: BT_2089

Alternate gene names: 29347499

Gene position: 2617983-2615821 (Counterclockwise)

Preceding gene: 29347500

Following gene: 29347498

Centisome position: 41.82

GC content: 47.3

Gene sequence:

>2163_bases
ATGATAGTTTGCATAGCCGAAAAGCCGTCTGTAGCACGTGATATAGCCGAAGTACTCGGCGCTCATACAAGAAAAGAAGG
GTATATAGAAGGAAACGGATACCAGGTAACCTGGACATTCGGTCATCTTTGTACCTTGAAAGAGCCGCATGAATACACCC
CTAACTGGAAATCATGGAACTTGGGTAGCTTACCGATGATTCCGCCACGGTTCGGCATCAAGCTGATAGAGAACCCTACT
TACGAAAAACAGTTTCACATTATCGAGGGACTGATGCAGAATGCAGATGAAATCATCAACTGCGGTGATGCCGGCCAAGA
AGGAGAATTGATCCAGCGTTGGGTGATGCAGAAGGCAGGTGCCCGCTGTCCGGTGAAACGTCTGTGGATTTCTTCGCTGA
CAGAAGAGGCGATCCGCGAAGGATTTGCCAAACTAAAGGATCAGTCCGACTTTCAATCACTGTATGAAGCGGGACTTTCC
CGTGCAATGGGGGACTGGTTGCTGGGAATGAATGCTACCCGTCTTTATACTATCAAATACGGTCAGAACAAACAAGTGCT
TTCCATCGGTCGTGTGCAGACTCCTACGCTGGCATTGATTGTCAACCGTCAACTGGAGATCGCCAACTTCCAGCCGAAAC
AATACTGGGAACTGAAAACAAATTATCGTGATACCACCTTTTCTGCTCTGATCCGTAAAAGCGATGAAGAAATTGCTGCG
GAAGAAGAAAAGAGTGGCGGAAAGAAGAAGATAGACAATCCCGGCATCGACCCTATCGCCAACCGGGAGGAGGGAGAAGC
GCTGGTGGAACGCATAAAAGACCTTCCGTTCATTGTCACCAATGTCGGCAAGAAGGATGGAAAGGAATATGCTCCGCGTC
TGTTCGACCTGACTTCCCTTCAGGTAGAGTGTAATAAGAAGTTTGCCTATTCCGCTGATGAAACGCTGAAGCTGATTCAG
TCGCTTTATGAAAAGAAAGTGGCTACTTATCCGCGTGTCGATACCACTTTCCTGAGTGACGATATTTATCCGAAGTGTCC
CGGAATATTGAAAGGCCTTCGGGACTATGAGGTGCTGACTGCTCCTTTGTCAGGTGCTTCATTGCCTAAATCGAAAAAGG
TATTTGATAATTCGAAGGTAACAGATCACCATGCCATTATCCCTACAGGCGTTTATGCACAGAATCTGACGGATATGGAA
CGTCGTGTCTATGACCTGATAGCCCGTCGTTTTATAGCGGTGTTCTATCCCGATTGTAAGATTTCTACCACTACCGTGAT
GGGCGAAGTGGACAAGATAGAATTTAAAGTAATCGGCAAACAGATTCTGGAGCCGGGATGGCGTGTCGTTTTTGCCAAAG
ATGTGAAAGATACTTCCGAAGAAAAAGAGGAAGAGGATGAAAATGTACTTCCTGCCTTTGTCAAGGGGGAGAGTGGTCCT
CATGTTCCGGACTTGAATGAGAAGTGGACGCAACCGCCCAGACCTTATACGGAAGCAACATTGCTTCGTGCGATGGAAAC
AGCCGGAAAGTTGGTGGATAATGACGAACTCCGTGATGCTCTGAAGGAGAATGGTATCGGTCGGCCGTCTACCCGTGCCG
CCATTATCGAGACGTTGTTCAAGCGAAACTATATCCGCAAGGAAAGAAAGAACCTGATAGCCACCCCTACGGGAGTGGAA
CTGGTACAGATCATCCACGAAGAACTGCTGAAATCTGCGGAACTGACCGGTATATGGGAAAAGAAACTACGTGAGATTGA
AAAGAAGACATACGATGCCCGTCAATTCCTTGAAGAACTGAAGCAAATGGTATCTGAAATCGTGATGAGCGTACTTTCAG
ATAATACGAACCGACGTATCACTATTCAGGAGGCAGTTGCCCAAACGGAAGAAAAGGCAAAGAAAGAACCTAAGAAGCGT
GAGCGTAAAGCAAGTGCTCCCAAAGAAAAGAAGGCGAAAGCAGAACCGAAAGTTCCTGCATCTTCTCCGTCTCCTGCTCT
GACTCCTTCTGCTCCTGCCTCTGCTCCGGCTACCGGTAATGCAGATACTTTTGTCGGACAACCGTGTCCGGTTTGTGGTA
AGGGAACCATCATTAAAGGGAAGACAGCTTACGGATGTTCGGAATGGAGAAATGGATGTACTTACCGAAAGAATTTCGAA
TAG

Upstream 100 bases:

>100_bases
TTCGGGCTGAAGGCGGGATGTTTTTTTCATCTTTATGCGGTCAGTGAGAAATAATCATGTAACTTTGTAGACTGTTAGTA
TAAAAACAAGAAGAGAAAAA

Downstream 100 bases:

>100_bases
TTTGTGGACAGTGTCTCACATAACAGTGATTTCTCTTTTTTCAAAGAAAAAGAGTCCCTTCTTTTCTGTTCGCACCATAA
GGAATTAGATAGCCGTTTTG

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MIVCIAEKPSVARDIAEVLGAHTRKEGYIEGNGYQVTWTFGHLCTLKEPHEYTPNWKSWNLGSLPMIPPRFGIKLIENPT
YEKQFHIIEGLMQNADEIINCGDAGQEGELIQRWVMQKAGARCPVKRLWISSLTEEAIREGFAKLKDQSDFQSLYEAGLS
RAMGDWLLGMNATRLYTIKYGQNKQVLSIGRVQTPTLALIVNRQLEIANFQPKQYWELKTNYRDTTFSALIRKSDEEIAA
EEEKSGGKKKIDNPGIDPIANREEGEALVERIKDLPFIVTNVGKKDGKEYAPRLFDLTSLQVECNKKFAYSADETLKLIQ
SLYEKKVATYPRVDTTFLSDDIYPKCPGILKGLRDYEVLTAPLSGASLPKSKKVFDNSKVTDHHAIIPTGVYAQNLTDME
RRVYDLIARRFIAVFYPDCKISTTTVMGEVDKIEFKVIGKQILEPGWRVVFAKDVKDTSEEKEEEDENVLPAFVKGESGP
HVPDLNEKWTQPPRPYTEATLLRAMETAGKLVDNDELRDALKENGIGRPSTRAAIIETLFKRNYIRKERKNLIATPTGVE
LVQIIHEELLKSAELTGIWEKKLREIEKKTYDARQFLEELKQMVSEIVMSVLSDNTNRRITIQEAVAQTEEKAKKEPKKR
ERKASAPKEKKAKAEPKVPASSPSPALTPSAPASAPATGNADTFVGQPCPVCGKGTIIKGKTAYGCSEWRNGCTYRKNFE

Sequences:

>Translated_720_residues
MIVCIAEKPSVARDIAEVLGAHTRKEGYIEGNGYQVTWTFGHLCTLKEPHEYTPNWKSWNLGSLPMIPPRFGIKLIENPT
YEKQFHIIEGLMQNADEIINCGDAGQEGELIQRWVMQKAGARCPVKRLWISSLTEEAIREGFAKLKDQSDFQSLYEAGLS
RAMGDWLLGMNATRLYTIKYGQNKQVLSIGRVQTPTLALIVNRQLEIANFQPKQYWELKTNYRDTTFSALIRKSDEEIAA
EEEKSGGKKKIDNPGIDPIANREEGEALVERIKDLPFIVTNVGKKDGKEYAPRLFDLTSLQVECNKKFAYSADETLKLIQ
SLYEKKVATYPRVDTTFLSDDIYPKCPGILKGLRDYEVLTAPLSGASLPKSKKVFDNSKVTDHHAIIPTGVYAQNLTDME
RRVYDLIARRFIAVFYPDCKISTTTVMGEVDKIEFKVIGKQILEPGWRVVFAKDVKDTSEEKEEEDENVLPAFVKGESGP
HVPDLNEKWTQPPRPYTEATLLRAMETAGKLVDNDELRDALKENGIGRPSTRAAIIETLFKRNYIRKERKNLIATPTGVE
LVQIIHEELLKSAELTGIWEKKLREIEKKTYDARQFLEELKQMVSEIVMSVLSDNTNRRITIQEAVAQTEEKAKKEPKKR
ERKASAPKEKKAKAEPKVPASSPSPALTPSAPASAPATGNADTFVGQPCPVCGKGTIIKGKTAYGCSEWRNGCTYRKNFE
>Mature_720_residues
MIVCIAEKPSVARDIAEVLGAHTRKEGYIEGNGYQVTWTFGHLCTLKEPHEYTPNWKSWNLGSLPMIPPRFGIKLIENPT
YEKQFHIIEGLMQNADEIINCGDAGQEGELIQRWVMQKAGARCPVKRLWISSLTEEAIREGFAKLKDQSDFQSLYEAGLS
RAMGDWLLGMNATRLYTIKYGQNKQVLSIGRVQTPTLALIVNRQLEIANFQPKQYWELKTNYRDTTFSALIRKSDEEIAA
EEEKSGGKKKIDNPGIDPIANREEGEALVERIKDLPFIVTNVGKKDGKEYAPRLFDLTSLQVECNKKFAYSADETLKLIQ
SLYEKKVATYPRVDTTFLSDDIYPKCPGILKGLRDYEVLTAPLSGASLPKSKKVFDNSKVTDHHAIIPTGVYAQNLTDME
RRVYDLIARRFIAVFYPDCKISTTTVMGEVDKIEFKVIGKQILEPGWRVVFAKDVKDTSEEKEEEDENVLPAFVKGESGP
HVPDLNEKWTQPPRPYTEATLLRAMETAGKLVDNDELRDALKENGIGRPSTRAAIIETLFKRNYIRKERKNLIATPTGVE
LVQIIHEELLKSAELTGIWEKKLREIEKKTYDARQFLEELKQMVSEIVMSVLSDNTNRRITIQEAVAQTEEKAKKEPKKR
ERKASAPKEKKAKAEPKVPASSPSPALTPSAPASAPATGNADTFVGQPCPVCGKGTIIKGKTAYGCSEWRNGCTYRKNFE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=577, Percent_Identity=23.2235701906412, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI4507635, Length=643, Percent_Identity=25.8164852255054, Blast_Score=95, Evalue=3e-19,
Organism=Escherichia coli, GI1788061, Length=678, Percent_Identity=33.3333333333333, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1787529, Length=536, Percent_Identity=26.1194029850746, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32563869, Length=600, Percent_Identity=25.5, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323263, Length=640, Percent_Identity=25.15625, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24640096, Length=605, Percent_Identity=23.1404958677686, Blast_Score=92, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 81144; Mature: 81144

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVCIAEKPSVARDIAEVLGAHTRKEGYIEGNGYQVTWTFGHLCTLKEPHEYTPNWKSWN
CEEEEECCCHHHHHHHHHHCCCCCCCCEEECCCEEEEEEECCEEECCCCCCCCCCCCCCC
LGSLPMIPPRFGIKLIENPTYEKQFHIIEGLMQNADEIINCGDAGQEGELIQRWVMQKAG
CCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHCC
ARCPVKRLWISSLTEEAIREGFAKLKDQSDFQSLYEAGLSRAMGDWLLGMNATRLYTIKY
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
GQNKQVLSIGRVQTPTLALIVNRQLEIANFQPKQYWELKTNYRDTTFSALIRKSDEEIAA
CCCCCEEEECCCCCCEEEEEEECCEEECCCCCCHHEEEECCCCHHHHHHHHHCCCHHHHH
EEEKSGGKKKIDNPGIDPIANREEGEALVERIKDLPFIVTNVGKKDGKEYAPRLFDLTSL
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCHHHCCHHHHHHEE
QVECNKKFAYSADETLKLIQSLYEKKVATYPRVDTTFLSDDIYPKCPGILKGLRDYEVLT
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHHCCCCCCEEE
APLSGASLPKSKKVFDNSKVTDHHAIIPTGVYAQNLTDMERRVYDLIARRFIAVFYPDCK
CCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ISTTTVMGEVDKIEFKVIGKQILEPGWRVVFAKDVKDTSEEKEEEDENVLPAFVKGESGP
EEHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCCCEEECCCCC
HVPDLNEKWTQPPRPYTEATLLRAMETAGKLVDNDELRDALKENGIGRPSTRAAIIETLF
CCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
KRNYIRKERKNLIATPTGVELVQIIHEELLKSAELTGIWEKKLREIEKKTYDARQFLEEL
HHHHHHHHHHCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQMVSEIVMSVLSDNTNRRITIQEAVAQTEEKAKKEPKKRERKASAPKEKKAKAEPKVPA
HHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHCCCCCCCCC
SSPSPALTPSAPASAPATGNADTFVGQPCPVCGKGTIIKGKTAYGCSEWRNGCTYRKNFE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIVCIAEKPSVARDIAEVLGAHTRKEGYIEGNGYQVTWTFGHLCTLKEPHEYTPNWKSWN
CEEEEECCCHHHHHHHHHHCCCCCCCCEEECCCEEEEEEECCEEECCCCCCCCCCCCCCC
LGSLPMIPPRFGIKLIENPTYEKQFHIIEGLMQNADEIINCGDAGQEGELIQRWVMQKAG
CCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHCC
ARCPVKRLWISSLTEEAIREGFAKLKDQSDFQSLYEAGLSRAMGDWLLGMNATRLYTIKY
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
GQNKQVLSIGRVQTPTLALIVNRQLEIANFQPKQYWELKTNYRDTTFSALIRKSDEEIAA
CCCCCEEEECCCCCCEEEEEEECCEEECCCCCCHHEEEECCCCHHHHHHHHHCCCHHHHH
EEEKSGGKKKIDNPGIDPIANREEGEALVERIKDLPFIVTNVGKKDGKEYAPRLFDLTSL
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCHHHCCHHHHHHEE
QVECNKKFAYSADETLKLIQSLYEKKVATYPRVDTTFLSDDIYPKCPGILKGLRDYEVLT
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHHCCCCCCEEE
APLSGASLPKSKKVFDNSKVTDHHAIIPTGVYAQNLTDMERRVYDLIARRFIAVFYPDCK
CCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ISTTTVMGEVDKIEFKVIGKQILEPGWRVVFAKDVKDTSEEKEEEDENVLPAFVKGESGP
EEHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCCCEEECCCCC
HVPDLNEKWTQPPRPYTEATLLRAMETAGKLVDNDELRDALKENGIGRPSTRAAIIETLF
CCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
KRNYIRKERKNLIATPTGVELVQIIHEELLKSAELTGIWEKKLREIEKKTYDARQFLEEL
HHHHHHHHHHCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQMVSEIVMSVLSDNTNRRITIQEAVAQTEEKAKKEPKKRERKASAPKEKKAKAEPKVPA
HHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHCCCCCCCCC
SSPSPALTPSAPASAPATGNADTFVGQPCPVCGKGTIIKGKTAYGCSEWRNGCTYRKNFE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA