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The map label for this gene is purU [H]

Identifier: 29346791

GI number: 29346791

Start: 1708512

End: 1709366

Strand: Reverse

Name: purU [H]

Synonym: BT_1381

Alternate gene names: 29346791

Gene position: 1709366-1708512 (Counterclockwise)

Preceding gene: 29346793

Following gene: 29346790

Centisome position: 27.3

GC content: 41.52

Gene sequence:

>855_bases
ATGACTACAGCCAAACTGTTATTGCACTGTCCCGACAAACCGGGAATCCTCGCGGAAGTGACAGACTTTATTACGGTAAA
CAAAGGAAATATTATCTATCTGGATCAGTATGTAGACCATGTGGAGAACATCTTCTTCATGCGTATAGAATGGGAATTGA
AAGATTTTCTGGTTCCGCAGGAAAAGATTGAAGATTATTTCAGAACGCTATATGGGCAGAAGTATGAAATGGATTTCCGT
CTTTATTTCTCGGATGTGAAACCGCGTATGGCTATATTCGTTTCTAAAATGTCTCATTGCCTGTTCGATATGTTGGCTCG
CTACACGGCAGGAGAGTGGAACGTGGAGATACCTCTTATTATAAGCAACCATCCGGACTTGCAGCACGTGGCAGAGCGTT
TCGGTATTCCTTTCTATTTGTTCCCCATCACCAAGGAGACCAAAGAAGAACAGGAACGCAAAGAAATGGAATTGCTTGCC
AAACATAAAATTACATTCATCGTGTTGGCACGTTATATGCAAGTGATTTCCGAACAGATGATCAATGCGTATCCAAATAA
GATTATCAATATCCATCATTCCTTCCTACCGGCATTTGTGGGGGCAAAACCTTATCATGCCGCATTCCAGCGTGGAGTGA
AAATTATCGGTGCGACCAGCCATTATGTGACGACTGAGCTGGATGCAGGTCCGATCATCGAGCAGGATGTAGTACGCATT
ACACATAAGGACTCTATAGAAGATCTTGTAAATAAAGGAAAGGACCTGGAAAAAATTGTTCTTTCGCGTGCGGTACAAAA
GCATATCGAACGTAAGATTTTGGCTTATAAAAATAAAACAGTAATATTTAGCTGA

Upstream 100 bases:

>100_bases
TGAATAATTCGTAGTTTTGCAATAAATCGTTATTTTTGCGCACTAAAATTAGATAGGTGGAAGAATTTCCGCCCGGAATA
GTAAATTAGTAAAAAGAATG

Downstream 100 bases:

>100_bases
TGAAAGTAGCAGTTGTAAAATACAATGCCGGGAATATCCGTTCTGTGGACTATGCTCTGAAGCGGTTGGGAGTAGAAGCA
GTGATTACTGCCGATAAAGA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTTAKLLLHCPDKPGILAEVTDFITVNKGNIIYLDQYVDHVENIFFMRIEWELKDFLVPQEKIEDYFRTLYGQKYEMDFR
LYFSDVKPRMAIFVSKMSHCLFDMLARYTAGEWNVEIPLIISNHPDLQHVAERFGIPFYLFPITKETKEEQERKEMELLA
KHKITFIVLARYMQVISEQMINAYPNKIINIHHSFLPAFVGAKPYHAAFQRGVKIIGATSHYVTTELDAGPIIEQDVVRI
THKDSIEDLVNKGKDLEKIVLSRAVQKHIERKILAYKNKTVIFS

Sequences:

>Translated_284_residues
MTTAKLLLHCPDKPGILAEVTDFITVNKGNIIYLDQYVDHVENIFFMRIEWELKDFLVPQEKIEDYFRTLYGQKYEMDFR
LYFSDVKPRMAIFVSKMSHCLFDMLARYTAGEWNVEIPLIISNHPDLQHVAERFGIPFYLFPITKETKEEQERKEMELLA
KHKITFIVLARYMQVISEQMINAYPNKIINIHHSFLPAFVGAKPYHAAFQRGVKIIGATSHYVTTELDAGPIIEQDVVRI
THKDSIEDLVNKGKDLEKIVLSRAVQKHIERKILAYKNKTVIFS
>Mature_283_residues
TTAKLLLHCPDKPGILAEVTDFITVNKGNIIYLDQYVDHVENIFFMRIEWELKDFLVPQEKIEDYFRTLYGQKYEMDFRL
YFSDVKPRMAIFVSKMSHCLFDMLARYTAGEWNVEIPLIISNHPDLQHVAERFGIPFYLFPITKETKEEQERKEMELLAK
HKITFIVLARYMQVISEQMINAYPNKIINIHHSFLPAFVGAKPYHAAFQRGVKIIGATSHYVTTELDAGPIIEQDVVRIT
HKDSIEDLVNKGKDLEKIVLSRAVQKHIERKILAYKNKTVIFS

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=191, Percent_Identity=28.2722513089005, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI209869995, Length=191, Percent_Identity=28.2722513089005, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI209869993, Length=191, Percent_Identity=28.2722513089005, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787483, Length=274, Percent_Identity=41.2408759124088, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI1788846, Length=107, Percent_Identity=29.9065420560748, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17567511, Length=166, Percent_Identity=31.3253012048193, Blast_Score=91, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33197; Mature: 33066

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAKLLLHCPDKPGILAEVTDFITVNKGNIIYLDQYVDHVENIFFMRIEWELKDFLVPQ
CCCCEEEEECCCCCCHHHHHHHHEEECCCCEEEEHHHHHHHCCEEEEEEEEEHHHHCCCH
EKIEDYFRTLYGQKYEMDFRLYFSDVKPRMAIFVSKMSHCLFDMLARYTAGEWNVEIPLI
HHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
ISNHPDLQHVAERFGIPFYLFPITKETKEEQERKEMELLAKHKITFIVLARYMQVISEQM
ECCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INAYPNKIINIHHSFLPAFVGAKPYHAAFQRGVKIIGATSHYVTTELDAGPIIEQDVVRI
HHHCCHHHEEHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCEEEEECCCCCCCHHHHHHH
THKDSIEDLVNKGKDLEKIVLSRAVQKHIERKILAYKNKTVIFS
HCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure 
TTAKLLLHCPDKPGILAEVTDFITVNKGNIIYLDQYVDHVENIFFMRIEWELKDFLVPQ
CCCEEEEECCCCCCHHHHHHHHEEECCCCEEEEHHHHHHHCCEEEEEEEEEHHHHCCCH
EKIEDYFRTLYGQKYEMDFRLYFSDVKPRMAIFVSKMSHCLFDMLARYTAGEWNVEIPLI
HHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
ISNHPDLQHVAERFGIPFYLFPITKETKEEQERKEMELLAKHKITFIVLARYMQVISEQM
ECCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INAYPNKIINIHHSFLPAFVGAKPYHAAFQRGVKIIGATSHYVTTELDAGPIIEQDVVRI
HHHCCHHHEEHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCEEEEECCCCCCCHHHHHHH
THKDSIEDLVNKGKDLEKIVLSRAVQKHIERKILAYKNKTVIFS
HCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]