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The map label for this gene is lipB [H]

Identifier: 29346777

GI number: 29346777

Start: 1696087

End: 1696845

Strand: Reverse

Name: lipB [H]

Synonym: BT_1367

Alternate gene names: 29346777

Gene position: 1696845-1696087 (Counterclockwise)

Preceding gene: 29346778

Following gene: 29346776

Centisome position: 27.1

GC content: 50.33

Gene sequence:

>759_bases
ATGAAAGTAAGAATACTTGGAAGCGGAACATCGACGGGAGTTCCTCAGATTGGATGTACCTGTCCGGTCTGCACTTCTGC
CGATCCGAAAGATAATCGTCTGCGTGCGTCTGCGATCGTTGAGACGGAAGATGCGCGGATACTTATAGACTGCGGGCCCG
ATTTTCGGGCGCAGGTGTTACACCTTCCTTTTGAGAAGATAGACGGTGTGCTCATTACGCACGAACATTATGACCACGTG
GGCGGGCTGGATGATTTGCGTCCGTTTTGCCGCTTCGGGGCGGTTCCTATCTATGCCGAAGAATATGTGGCACGCGCATT
GCGTTTGCGGATGCCTTATTGTTTTGTCGATCACCGTTATCCCGGTGTTCCTGATATTCCCTTGCAGGAAATTGAGGTCG
GGCACGCCTTTTCCATCCACCATACGGAAGTGGTGCCTTTGCGGGTGATGCATGGTCGGCTGCCGATTCTGGGCTACCGT
ATCGGCAGGCTGGGGTATATCACGGATATGCTGACCATGCCCGAAGAATCGTATGAACAATTGGAAGGGATAGATGTTCT
GATTATGAATGCGCTTCGTATCGCTCCTCATCCTACTCACCAGAATCTGGAGGAGGCATTGAAGGTGGCAGAACGTATTC
GGGCAAAGGAGACATACTTCATCCACATGAGTCATGATATGGGGCTGCACAAGAAGGTAGAGAAGGAGCTTCCTGAAAAT
ATTCACCTTACATATGACGGAATGGAAATCATTTTCTGA

Upstream 100 bases:

>100_bases
CGAAAGGCAGCGACGTCCTTGCGCTTTCGGATGCAGTGCGGGCTTCTGTTCGTGCGAAATTCGGCATAGACATTCATCCG
GAAGTAAATTTGGTTGGAAA

Downstream 100 bases:

>100_bases
TTATTGGTGATTTTTTTAGCAAAAGTTTTGTTTTCTTTGTAGAAATTATTATTTTTGCCCCTAGCCGATAAACAGCAAAT
ATTATGAAACTTCGTACGAT

Product: putative hydrolase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKVRILGSGTSTGVPQIGCTCPVCTSADPKDNRLRASAIVETEDARILIDCGPDFRAQVLHLPFEKIDGVLITHEHYDHV
GGLDDLRPFCRFGAVPIYAEEYVARALRLRMPYCFVDHRYPGVPDIPLQEIEVGHAFSIHHTEVVPLRVMHGRLPILGYR
IGRLGYITDMLTMPEESYEQLEGIDVLIMNALRIAPHPTHQNLEEALKVAERIRAKETYFIHMSHDMGLHKKVEKELPEN
IHLTYDGMEIIF

Sequences:

>Translated_252_residues
MKVRILGSGTSTGVPQIGCTCPVCTSADPKDNRLRASAIVETEDARILIDCGPDFRAQVLHLPFEKIDGVLITHEHYDHV
GGLDDLRPFCRFGAVPIYAEEYVARALRLRMPYCFVDHRYPGVPDIPLQEIEVGHAFSIHHTEVVPLRVMHGRLPILGYR
IGRLGYITDMLTMPEESYEQLEGIDVLIMNALRIAPHPTHQNLEEALKVAERIRAKETYFIHMSHDMGLHKKVEKELPEN
IHLTYDGMEIIF
>Mature_252_residues
MKVRILGSGTSTGVPQIGCTCPVCTSADPKDNRLRASAIVETEDARILIDCGPDFRAQVLHLPFEKIDGVLITHEHYDHV
GGLDDLRPFCRFGAVPIYAEEYVARALRLRMPYCFVDHRYPGVPDIPLQEIEVGHAFSIHHTEVVPLRVMHGRLPILGYR
IGRLGYITDMLTMPEESYEQLEGIDVLIMNALRIAPHPTHQNLEEALKVAERIRAKETYFIHMSHDMGLHKKVEKELPEN
IHLTYDGMEIIF

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG1235

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI1790530, Length=254, Percent_Identity=27.9527559055118, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF00753 Lactamase_B [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 28613; Mature: 28613

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRILGSGTSTGVPQIGCTCPVCTSADPKDNRLRASAIVETEDARILIDCGPDFRAQVL
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEECCCCCHHHEE
HLPFEKIDGVLITHEHYDHVGGLDDLRPFCRFGAVPIYAEEYVARALRLRMPYCFVDHRY
ECCHHHHCCEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCC
PGVPDIPLQEIEVGHAFSIHHTEVVPLRVMHGRLPILGYRIGRLGYITDMLTMPEESYEQ
CCCCCCCHHHCCCCCEEEECCCEEEEEEEECCCCCEEEEEHHHHHHHHHHHHCCHHHHHH
LEGIDVLIMNALRIAPHPTHQNLEEALKVAERIRAKETYFIHMSHDMGLHKKVEKELPEN
HCCCHHEEEHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCC
IHLTYDGMEIIF
EEEEECCEEEEC
>Mature Secondary Structure
MKVRILGSGTSTGVPQIGCTCPVCTSADPKDNRLRASAIVETEDARILIDCGPDFRAQVL
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEECCCCCHHHEE
HLPFEKIDGVLITHEHYDHVGGLDDLRPFCRFGAVPIYAEEYVARALRLRMPYCFVDHRY
ECCHHHHCCEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCC
PGVPDIPLQEIEVGHAFSIHHTEVVPLRVMHGRLPILGYRIGRLGYITDMLTMPEESYEQ
CCCCCCCHHHCCCCCEEEECCCEEEEEEEECCCCCEEEEEHHHHHHHHHHHHCCHHHHHH
LEGIDVLIMNALRIAPHPTHQNLEEALKVAERIRAKETYFIHMSHDMGLHKKVEKELPEN
HCCCHHEEEHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCC
IHLTYDGMEIIF
EEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]