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The map label for this gene is tgt [H]

Identifier: 29346245

GI number: 29346245

Start: 1030316

End: 1031446

Strand: Reverse

Name: tgt [H]

Synonym: BT_0835

Alternate gene names: 29346245

Gene position: 1031446-1030316 (Counterclockwise)

Preceding gene: 29346246

Following gene: 29346244

Centisome position: 16.48

GC content: 45.98

Gene sequence:

>1131_bases
ATGACATTTGAGTTACAATATACAGACACTAAAAGTAATGCCCGTGCAGGTCTGATAACAACAGACCACGGGCAGATACA
AACACCTATCTTCATGCCTGTGGGAACACTGGGCACTGTTAAAGGAGTACATGTAACAGAACTGAAAGAAGATATCCAGG
CACAGATTATTCTGGGCAACACCTATCATTTATACTTGCGTCCGGGACTAGACGTAATTGAAAAGGCAGGCGGACTGCAT
CGCTTCAATGGTTTTGATCGTCCTATGCTGACCGATAGCGGTGGTTTTCAGGTATTCTCATTAGCAGGAATACGAAAACT
TCGGGAAGAAGGAGCCGAATTCCGCTCGCACATTGATGGCAGCAAACATATCTTTACCCCGGAAAAGGTGATGGATATAG
AACGTACCATTGGCGCTGATATCATGATGGCTTTTGACGAATGTCCTCCGGGAGATTCAGATTATGCATATGCCAAAAAG
TCATTAGGGCTGACACACAGATGGCTTGATCGTTGTATTCAGCGTTTTAACGAAACTGAACCGAAATACGGCTACAACCA
GTCTCTCTTCCCCATCGTGCAAGGATGTGTTTATCAGGATTTGCGTAAGCAATCCGCTGAATTCGTCGCCTCCAAAGGAG
CGGATGGAAATGCAATCGGTGGACTGGCCGTAGGCGAACCGGTAGATAAGATGTACGAGATGATAGAGGTTGTCAACGAG
ATTCTTCCCAAAGACAAGCCCCGCTATCTGATGGGAGTCGGTACCCCCGTAAATATCCTTGAAGGCATTGAACGCGGAGT
CGATATGTTCGACTGTGTTATGCCTACCCGAAACGGACGAAATGGCATGCTGTTTACCAAAGACGGTATCATCAATATGC
GAAACAAGAAATGGGAAACAGACTTCTCTCCCATCGAGGCAGACGGTGCTTCTGCTGTAGACACGTTATACAGCAAAGCG
TACTTGCGTCACCTTTTCCACGCACAAGAACTACTGGCAATGCAAATTGCTTCTATACACAATCTGGCATTTTACCTGTG
GCTGGTAGGTGAAGCCCGGAAACATATCATTGCCGGAGATTTCTCTACCTGGAAGCCGATGATGGTAAAAAGAGTGTCAA
CAAGATTATAA

Upstream 100 bases:

>100_bases
TCCACTATGTAAACGACATTAAAGAAGTATTCGCAATCGCCTTGACAAACGAGAAAGTTGCGGATGCCATTGATTTATCT
GTAAAGAAACCCAGCCAGGA

Downstream 100 bases:

>100_bases
AGAATGAAAAGCAATCGATTAATAAAACGGCTGGATTGGTATATCATCAAGAAATTCTTGGGGACATACGTATTTGCCAT
TGCATTGATTATCTCAATTG

Product: tRNA-guanine transglycosylase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTFELQYTDTKSNARAGLITTDHGQIQTPIFMPVGTLGTVKGVHVTELKEDIQAQIILGNTYHLYLRPGLDVIEKAGGLH
RFNGFDRPMLTDSGGFQVFSLAGIRKLREEGAEFRSHIDGSKHIFTPEKVMDIERTIGADIMMAFDECPPGDSDYAYAKK
SLGLTHRWLDRCIQRFNETEPKYGYNQSLFPIVQGCVYQDLRKQSAEFVASKGADGNAIGGLAVGEPVDKMYEMIEVVNE
ILPKDKPRYLMGVGTPVNILEGIERGVDMFDCVMPTRNGRNGMLFTKDGIINMRNKKWETDFSPIEADGASAVDTLYSKA
YLRHLFHAQELLAMQIASIHNLAFYLWLVGEARKHIIAGDFSTWKPMMVKRVSTRL

Sequences:

>Translated_376_residues
MTFELQYTDTKSNARAGLITTDHGQIQTPIFMPVGTLGTVKGVHVTELKEDIQAQIILGNTYHLYLRPGLDVIEKAGGLH
RFNGFDRPMLTDSGGFQVFSLAGIRKLREEGAEFRSHIDGSKHIFTPEKVMDIERTIGADIMMAFDECPPGDSDYAYAKK
SLGLTHRWLDRCIQRFNETEPKYGYNQSLFPIVQGCVYQDLRKQSAEFVASKGADGNAIGGLAVGEPVDKMYEMIEVVNE
ILPKDKPRYLMGVGTPVNILEGIERGVDMFDCVMPTRNGRNGMLFTKDGIINMRNKKWETDFSPIEADGASAVDTLYSKA
YLRHLFHAQELLAMQIASIHNLAFYLWLVGEARKHIIAGDFSTWKPMMVKRVSTRL
>Mature_375_residues
TFELQYTDTKSNARAGLITTDHGQIQTPIFMPVGTLGTVKGVHVTELKEDIQAQIILGNTYHLYLRPGLDVIEKAGGLHR
FNGFDRPMLTDSGGFQVFSLAGIRKLREEGAEFRSHIDGSKHIFTPEKVMDIERTIGADIMMAFDECPPGDSDYAYAKKS
LGLTHRWLDRCIQRFNETEPKYGYNQSLFPIVQGCVYQDLRKQSAEFVASKGADGNAIGGLAVGEPVDKMYEMIEVVNEI
LPKDKPRYLMGVGTPVNILEGIERGVDMFDCVMPTRNGRNGMLFTKDGIINMRNKKWETDFSPIEADGASAVDTLYSKAY
LRHLFHAQELLAMQIASIHNLAFYLWLVGEARKHIIAGDFSTWKPMMVKRVSTRL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=352, Percent_Identity=39.4886363636364, Blast_Score=271, Evalue=1e-72,
Organism=Escherichia coli, GI1786607, Length=364, Percent_Identity=48.0769230769231, Blast_Score=344, Evalue=5e-96,
Organism=Caenorhabditis elegans, GI17544680, Length=344, Percent_Identity=37.5, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI25146502, Length=125, Percent_Identity=30.4, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI19920510, Length=364, Percent_Identity=40.3846153846154, Blast_Score=287, Evalue=7e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42224; Mature: 42093

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFELQYTDTKSNARAGLITTDHGQIQTPIFMPVGTLGTVKGVHVTELKEDIQAQIILGN
CEEEEEECCCCCCCCCCEEECCCCCEECCEEEECCCCCCCCCCHHHHHHHCCEEEEEECC
TYHLYLRPGLDVIEKAGGLHRFNGFDRPMLTDSGGFQVFSLAGIRKLREEGAEFRSHIDG
EEEEEECCCHHHHHHCCCCCCCCCCCCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHCCC
SKHIFTPEKVMDIERTIGADIMMAFDECPPGDSDYAYAKKSLGLTHRWLDRCIQRFNETE
CCEEECHHHHHHHHHHHCHHHEEHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCC
PKYGYNQSLFPIVQGCVYQDLRKQSAEFVASKGADGNAIGGLAVGEPVDKMYEMIEVVNE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ILPKDKPRYLMGVGTPVNILEGIERGVDMFDCVMPTRNGRNGMLFTKDGIINMRNKKWET
HCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEECCCEEECCCCCCCC
DFSPIEADGASAVDTLYSKAYLRHLFHAQELLAMQIASIHNLAFYLWLVGEARKHIIAGD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECC
FSTWKPMMVKRVSTRL
CCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TFELQYTDTKSNARAGLITTDHGQIQTPIFMPVGTLGTVKGVHVTELKEDIQAQIILGN
EEEEEECCCCCCCCCCEEECCCCCEECCEEEECCCCCCCCCCHHHHHHHCCEEEEEECC
TYHLYLRPGLDVIEKAGGLHRFNGFDRPMLTDSGGFQVFSLAGIRKLREEGAEFRSHIDG
EEEEEECCCHHHHHHCCCCCCCCCCCCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHCCC
SKHIFTPEKVMDIERTIGADIMMAFDECPPGDSDYAYAKKSLGLTHRWLDRCIQRFNETE
CCEEECHHHHHHHHHHHCHHHEEHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCC
PKYGYNQSLFPIVQGCVYQDLRKQSAEFVASKGADGNAIGGLAVGEPVDKMYEMIEVVNE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ILPKDKPRYLMGVGTPVNILEGIERGVDMFDCVMPTRNGRNGMLFTKDGIINMRNKKWET
HCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEECCCEEECCCCCCCC
DFSPIEADGASAVDTLYSKAYLRHLFHAQELLAMQIASIHNLAFYLWLVGEARKHIIAGD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECC
FSTWKPMMVKRVSTRL
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA