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The map label for this gene is barA [H]

Identifier: 29346064

GI number: 29346064

Start: 803832

End: 805814

Strand: Reverse

Name: barA [H]

Synonym: BT_0654

Alternate gene names: 29346064

Gene position: 805814-803832 (Counterclockwise)

Preceding gene: 29346065

Following gene: 29346063

Centisome position: 12.87

GC content: 44.18

Gene sequence:

>1983_bases
ATGACACTTCAGCTAAAAAATAAAAAATGGCGCATTGTCTACGTTATTTCCTTTCTTTTATTCTCCTTTACAGGCAATGC
GGCTATTCCGGAAGAACCGGAAGAGGAGATTCTTTTCGTGACGTCCTATAATTCCGATACTAAATATACCTACGATAATA
TCAACACGTTCGTAGAAACGTACCGCCAACTGGGTGGCAAGTATTCCACCATCGTAGAAAACATGAATGTTACCGACCTC
AATCAATCCAGAAAATGGAAAAAAAGACTTACCAACATTCTGGACAAACATCCGAACGCCAAGCTGGTTATCCTGCTGGG
TGGCGAAGCGTGGAGCAGTTTCCTGCACCTCGAAGACGAGAAGTACAGACAACTGCCCGTATTTTGCGCTATGGCATCGC
GCAACGGCATCCGTATTCCGGAAGACTCTATTGATATACGGACTTATGAACCTCAGAGTATCGACTTAACGGAAAGAATG
AACAAATACAATATAATATACTGTAACACATATGAATATGATATCGATAAGGACATAGAAATGATGCGAAGCTTTTATCC
GGATATGGAGCATTTAGTGTTCATCTCGGACAACACCTACAACGGACTTGCCGAACAAGCGTGGGTAAAAAAGAATATGA
AACGCTATCCGGAAATATCGACGACCTATATTGACGGCCGTATCCACACCCTTGATGCTGCCGCCAAACAACTACGCGAT
GTCCCCAAAAATTCGGTTGCACTACTGGGTATCTGGAGAATTGACAATCGGGGCATAACGTATATGAACAACTCTGTATA
TGCTTTCTCCAAAGCGAATCCAGAGTTGCCTGTTTTCAGCCTGACTGCTACCGCCATCGGTTATTGGGCAATCGGAGGAT
ATATCCCCCAATACGACGGAATAGGGAGAAGCATGGGAGAACAGGCTTATCAATTTCTGGACAAGGGGAAAAACAATGTC
GGCCACATTCATTTGTTGCCCAACAGATACAAATTTGATGCCAACAAACTGCATGAATGGGGATTTCAGGACAAGAAACT
ACCTTTCAATTCACTTATCATCAACCAACAGGTGCCTTTCTTTCAGGCATATCGGACGGAAGTGCAATTCATCCTGTTTA
CCTTCCTTGTACTGATAGGCGGACTGTTTATCTCACTGTATTATTATTACCGGACAAAAATCTTAAAGAATCACCTTGAG
AAGACTACAGCCCAACTAAGGGAAGATAAAAAGAAACTGGAATTATCGGAAATTGCACTACGTCATGCCAAAGAGCGTGC
CGAAGAAGCCAATCAACTGAAAAGTGCCTTCGTATCAAATATGAGCCACGAAATACGGACACCGCTGAACGCCATTGTCG
GCTTTTCCAGCCTGCTGATCGATACCGTGGAAACAACGGACGAGCAGAAGGAATATGCGGATATCATCCAGACTAATTCC
GAATTGTTATTGCAACTGATCAGTGATGTACTGGATGTATCACGTCTGGAATCAGGCAAACTGCTGTTCAAGTATGAATG
GTGCGAACTGGTAAATCATTGCCAGAACATGATCACACTGACCAGAAAGAACCGGACAAGAGATATAGATGTCAGGCTTC
AGATGCCGAAAGAGCCTTATATGCTCTATACCGATCCGCTACGTTTGCAGCAGGTGATCATCAACTTACTGAACAATGCC
CTGAAGTTTACTCCCGACGGCGGAAGTATCACGCTGGATTATGAAATAGACGAAGAAGACCAGTGTATGCTGTTCTCTGT
GACCGATACAGGCGCGGGCATCCCCGAAGACAAGCAGGAACTGGTATTCCAGCGTTTTGAGAAGCTCAACGAATTTGTGC
AGGGAACAGGCTTGGGACTGGCGATTTGCAAACTGACCATACAACGCATGGGAGGCGATATCTGGATTGACAAGAATTAT
AAGAACGGAGCACGGTTTGTCTTTTCTCATCCCATCAAAAAGCGGGAATCGGAAGAAAAATGA

Upstream 100 bases:

>100_bases
CACAGCGGCCTTTTTTCTAGCATAAAAAACTAAACTATCTATTGTATTTCAAAGGCATAATTTGTATTTTTGCAATAGCC
GAAAATCAAAACAGACAATT

Downstream 100 bases:

>100_bases
AAAAAAAACGGATAATTTGATGAATCTTTGTTTCTTTTTCGCTTACTTTGCTCCCGTAAAGACAAAGGGGTGCCCCCAAA
GAGGGGCTGAGATTATACCC

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MTLQLKNKKWRIVYVISFLLFSFTGNAAIPEEPEEEILFVTSYNSDTKYTYDNINTFVETYRQLGGKYSTIVENMNVTDL
NQSRKWKKRLTNILDKHPNAKLVILLGGEAWSSFLHLEDEKYRQLPVFCAMASRNGIRIPEDSIDIRTYEPQSIDLTERM
NKYNIIYCNTYEYDIDKDIEMMRSFYPDMEHLVFISDNTYNGLAEQAWVKKNMKRYPEISTTYIDGRIHTLDAAAKQLRD
VPKNSVALLGIWRIDNRGITYMNNSVYAFSKANPELPVFSLTATAIGYWAIGGYIPQYDGIGRSMGEQAYQFLDKGKNNV
GHIHLLPNRYKFDANKLHEWGFQDKKLPFNSLIINQQVPFFQAYRTEVQFILFTFLVLIGGLFISLYYYYRTKILKNHLE
KTTAQLREDKKKLELSEIALRHAKERAEEANQLKSAFVSNMSHEIRTPLNAIVGFSSLLIDTVETTDEQKEYADIIQTNS
ELLLQLISDVLDVSRLESGKLLFKYEWCELVNHCQNMITLTRKNRTRDIDVRLQMPKEPYMLYTDPLRLQQVIINLLNNA
LKFTPDGGSITLDYEIDEEDQCMLFSVTDTGAGIPEDKQELVFQRFEKLNEFVQGTGLGLAICKLTIQRMGGDIWIDKNY
KNGARFVFSHPIKKRESEEK

Sequences:

>Translated_660_residues
MTLQLKNKKWRIVYVISFLLFSFTGNAAIPEEPEEEILFVTSYNSDTKYTYDNINTFVETYRQLGGKYSTIVENMNVTDL
NQSRKWKKRLTNILDKHPNAKLVILLGGEAWSSFLHLEDEKYRQLPVFCAMASRNGIRIPEDSIDIRTYEPQSIDLTERM
NKYNIIYCNTYEYDIDKDIEMMRSFYPDMEHLVFISDNTYNGLAEQAWVKKNMKRYPEISTTYIDGRIHTLDAAAKQLRD
VPKNSVALLGIWRIDNRGITYMNNSVYAFSKANPELPVFSLTATAIGYWAIGGYIPQYDGIGRSMGEQAYQFLDKGKNNV
GHIHLLPNRYKFDANKLHEWGFQDKKLPFNSLIINQQVPFFQAYRTEVQFILFTFLVLIGGLFISLYYYYRTKILKNHLE
KTTAQLREDKKKLELSEIALRHAKERAEEANQLKSAFVSNMSHEIRTPLNAIVGFSSLLIDTVETTDEQKEYADIIQTNS
ELLLQLISDVLDVSRLESGKLLFKYEWCELVNHCQNMITLTRKNRTRDIDVRLQMPKEPYMLYTDPLRLQQVIINLLNNA
LKFTPDGGSITLDYEIDEEDQCMLFSVTDTGAGIPEDKQELVFQRFEKLNEFVQGTGLGLAICKLTIQRMGGDIWIDKNY
KNGARFVFSHPIKKRESEEK
>Mature_659_residues
TLQLKNKKWRIVYVISFLLFSFTGNAAIPEEPEEEILFVTSYNSDTKYTYDNINTFVETYRQLGGKYSTIVENMNVTDLN
QSRKWKKRLTNILDKHPNAKLVILLGGEAWSSFLHLEDEKYRQLPVFCAMASRNGIRIPEDSIDIRTYEPQSIDLTERMN
KYNIIYCNTYEYDIDKDIEMMRSFYPDMEHLVFISDNTYNGLAEQAWVKKNMKRYPEISTTYIDGRIHTLDAAAKQLRDV
PKNSVALLGIWRIDNRGITYMNNSVYAFSKANPELPVFSLTATAIGYWAIGGYIPQYDGIGRSMGEQAYQFLDKGKNNVG
HIHLLPNRYKFDANKLHEWGFQDKKLPFNSLIINQQVPFFQAYRTEVQFILFTFLVLIGGLFISLYYYYRTKILKNHLEK
TTAQLREDKKKLELSEIALRHAKERAEEANQLKSAFVSNMSHEIRTPLNAIVGFSSLLIDTVETTDEQKEYADIIQTNSE
LLLQLISDVLDVSRLESGKLLFKYEWCELVNHCQNMITLTRKNRTRDIDVRLQMPKEPYMLYTDPLRLQQVIINLLNNAL
KFTPDGGSITLDYEIDEEDQCMLFSVTDTGAGIPEDKQELVFQRFEKLNEFVQGTGLGLAICKLTIQRMGGDIWIDKNYK
NGARFVFSHPIKKRESEEK

Specific function: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=278, Percent_Identity=35.2517985611511, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI145693157, Length=251, Percent_Identity=38.2470119521912, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1788713, Length=242, Percent_Identity=32.2314049586777, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87081816, Length=275, Percent_Identity=34.9090909090909, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI48994928, Length=230, Percent_Identity=32.1739130434783, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1786912, Length=263, Percent_Identity=26.615969581749, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1790436, Length=283, Percent_Identity=24.3816254416961, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1786783, Length=247, Percent_Identity=25.9109311740891, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI87082128, Length=226, Percent_Identity=28.3185840707965, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788393, Length=232, Percent_Identity=25.4310344827586, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790861, Length=216, Percent_Identity=27.7777777777778, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790346, Length=229, Percent_Identity=27.5109170305677, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786600, Length=236, Percent_Identity=25, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787894, Length=229, Percent_Identity=26.6375545851528, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1788549, Length=245, Percent_Identity=24.4897959183673, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR019247
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF09984 DUF2222; PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 76705; Mature: 76574

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQLKNKKWRIVYVISFLLFSFTGNAAIPEEPEEEILFVTSYNSDTKYTYDNINTFVET
CEEEECCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCEEHHHHHHHHHH
YRQLGGKYSTIVENMNVTDLNQSRKWKKRLTNILDKHPNAKLVILLGGEAWSSFLHLEDE
HHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHEECCCH
KYRQLPVFCAMASRNGIRIPEDSIDIRTYEPQSIDLTERMNKYNIIYCNTYEYDIDKDIE
HHHCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHCCCEEEEEEEEEECCCCHHHH
MMRSFYPDMEHLVFISDNTYNGLAEQAWVKKNMKRYPEISTTYIDGRIHTLDAAAKQLRD
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCEEEEHHHHHHHHHH
VPKNSVALLGIWRIDNRGITYMNNSVYAFSKANPELPVFSLTATAIGYWAIGGYIPQYDG
CCCCCEEEEEEEEECCCCEEEECCCEEEEECCCCCCCEEEEHHHHHHHHHHCCCCCCCCC
IGRSMGEQAYQFLDKGKNNVGHIHLLPNRYKFDANKLHEWGFQDKKLPFNSLIINQQVPF
CCHHHHHHHHHHHHCCCCCCEEEEECCCCEECCHHHHHHCCCCCCCCCHHHEEECCCCCH
FQAYRTEVQFILFTFLVLIGGLFISLYYYYRTKILKNHLEKTTAQLREDKKKLELSEIAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHAKERAEEANQLKSAFVSNMSHEIRTPLNAIVGFSSLLIDTVETTDEQKEYADIIQTNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCH
ELLLQLISDVLDVSRLESGKLLFKYEWCELVNHCQNMITLTRKNRTRDIDVRLQMPKEPY
HHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHEECCCCCCEEEEEEEECCCCCE
MLYTDPLRLQQVIINLLNNALKFTPDGGSITLDYEIDEEDQCMLFSVTDTGAGIPEDKQE
EEEECHHHHHHHHHHHHHHHEEECCCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCHHH
LVFQRFEKLNEFVQGTGLGLAICKLTIQRMGGDIWIDKNYKNGARFVFSHPIKKRESEEK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TLQLKNKKWRIVYVISFLLFSFTGNAAIPEEPEEEILFVTSYNSDTKYTYDNINTFVET
EEEECCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCEEHHHHHHHHHH
YRQLGGKYSTIVENMNVTDLNQSRKWKKRLTNILDKHPNAKLVILLGGEAWSSFLHLEDE
HHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHEECCCH
KYRQLPVFCAMASRNGIRIPEDSIDIRTYEPQSIDLTERMNKYNIIYCNTYEYDIDKDIE
HHHCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHCCCEEEEEEEEEECCCCHHHH
MMRSFYPDMEHLVFISDNTYNGLAEQAWVKKNMKRYPEISTTYIDGRIHTLDAAAKQLRD
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCEEEEHHHHHHHHHH
VPKNSVALLGIWRIDNRGITYMNNSVYAFSKANPELPVFSLTATAIGYWAIGGYIPQYDG
CCCCCEEEEEEEEECCCCEEEECCCEEEEECCCCCCCEEEEHHHHHHHHHHCCCCCCCCC
IGRSMGEQAYQFLDKGKNNVGHIHLLPNRYKFDANKLHEWGFQDKKLPFNSLIINQQVPF
CCHHHHHHHHHHHHCCCCCCEEEEECCCCEECCHHHHHHCCCCCCCCCHHHEEECCCCCH
FQAYRTEVQFILFTFLVLIGGLFISLYYYYRTKILKNHLEKTTAQLREDKKKLELSEIAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHAKERAEEANQLKSAFVSNMSHEIRTPLNAIVGFSSLLIDTVETTDEQKEYADIIQTNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCH
ELLLQLISDVLDVSRLESGKLLFKYEWCELVNHCQNMITLTRKNRTRDIDVRLQMPKEPY
HHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHEECCCCCCEEEEEEEECCCCCE
MLYTDPLRLQQVIINLLNNALKFTPDGGSITLDYEIDEEDQCMLFSVTDTGAGIPEDKQE
EEEECHHHHHHHHHHHHHHHEEECCCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCHHH
LVFQRFEKLNEFVQGTGLGLAICKLTIQRMGGDIWIDKNYKNGARFVFSHPIKKRESEEK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]