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The map label for this gene is thiE

Identifier: 29346062

GI number: 29346062

Start: 802785

End: 803414

Strand: Reverse

Name: thiE

Synonym: BT_0652

Alternate gene names: 29346062

Gene position: 803414-802785 (Counterclockwise)

Preceding gene: 29346063

Following gene: 29346061

Centisome position: 12.83

GC content: 47.78

Gene sequence:

>630_bases
ATGGTCAGTCTACAATTTATCACCCACCAGACCGATCGGTATACTTATTTCGAATCGGCACTCATGGCACTTGAAGGAGG
ATGTAAGTGGATTCAGCTACGCATGAAGGAAGCTCCGTGTGAAGAGGTGGAAGCTGTTGCCCTCCAACTAAAGCCGCTCT
GCAAAGAAAAAGAAGCGATCTTACTTCTGGATGACCACGTCGAACTTGCCAAAAAGCTGGAAGTGGACGGAGTGCATCTG
GGCAAAAAAGACATGCCGATAGATCAGGCACGACAATTACTTGGAGAAGCATTTATTATCGGAGGTACGGCAAATACATT
CGAAGATGTCGTACAGCACTACCGTGCCGGAGCGGATTACCTCGGCATCGGTCCTTTCCGGTTTACCACTACAAAGAAAA
ACCTGAGTCCTGTACTGGGACTGGAAGGTTATACCGCTATTTTATCTCAGATGAAGGAAGCGAATATCGAACTTCCGGTA
GTAGCCATCGGAGGAATCACCCGTGAGGATATACCCGCCATACTCGAAACCGGAGTGAACGGAATCGCGCTTTCAGGAAC
GATTCTTCGGGCGGAGGATCCGGCAGCGGAAACACGAAAGATTTTGAACATGAAACGTATAATCAAATAA

Upstream 100 bases:

>100_bases
GTAAACAACAAAATGATTCCACGTTCCGAATGGGAGAAATTCGCGTTACAGGAGAATGATAACCTCGTGGTCATCAAAGC
GGCTTGTGGAGGGTAAAAGT

Downstream 100 bases:

>100_bases
ACTTATTGAAAAGTGGAAAAGTTAGTAATTGCAGGCCGTGAGTTCGACTCCCGCCTTTTTCTGGGTACAGGAAAATTCAA
TTCCAATGAAGTAATGGAAC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MVSLQFITHQTDRYTYFESALMALEGGCKWIQLRMKEAPCEEVEAVALQLKPLCKEKEAILLLDDHVELAKKLEVDGVHL
GKKDMPIDQARQLLGEAFIIGGTANTFEDVVQHYRAGADYLGIGPFRFTTTKKNLSPVLGLEGYTAILSQMKEANIELPV
VAIGGITREDIPAILETGVNGIALSGTILRAEDPAAETRKILNMKRIIK

Sequences:

>Translated_209_residues
MVSLQFITHQTDRYTYFESALMALEGGCKWIQLRMKEAPCEEVEAVALQLKPLCKEKEAILLLDDHVELAKKLEVDGVHL
GKKDMPIDQARQLLGEAFIIGGTANTFEDVVQHYRAGADYLGIGPFRFTTTKKNLSPVLGLEGYTAILSQMKEANIELPV
VAIGGITREDIPAILETGVNGIALSGTILRAEDPAAETRKILNMKRIIK
>Mature_209_residues
MVSLQFITHQTDRYTYFESALMALEGGCKWIQLRMKEAPCEEVEAVALQLKPLCKEKEAILLLDDHVELAKKLEVDGVHL
GKKDMPIDQARQLLGEAFIIGGTANTFEDVVQHYRAGADYLGIGPFRFTTTKKNLSPVLGLEGYTAILSQMKEANIELPV
VAIGGITREDIPAILETGVNGIALSGTILRAEDPAAETRKILNMKRIIK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=181, Percent_Identity=30.939226519337, Blast_Score=75, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=193, Percent_Identity=31.6062176165803, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_BACTN (Q8AA13)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_809565.1
- ProteinModelPortal:   Q8AA13
- GeneID:   1072399
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_0652
- NMPDR:   fig|226186.1.peg.652
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- PhylomeDB:   Q8AA13
- ProtClustDB:   PRK00043
- BioCyc:   BTHE226186:BT_0652-MONOMER
- BRENDA:   2.5.1.3
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23050; Mature: 23050

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLQFITHQTDRYTYFESALMALEGGCKWIQLRMKEAPCEEVEAVALQLKPLCKEKEAI
CEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEE
LLLDDHVELAKKLEVDGVHLGKKDMPIDQARQLLGEAFIIGGTANTFEDVVQHYRAGADY
EEEECHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCEEEECCCCHHHHHHHHHHHCCCCE
LGIGPFRFTTTKKNLSPVLGLEGYTAILSQMKEANIELPVVAIGGITREDIPAILETGVN
ECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCC
GIALSGTILRAEDPAAETRKILNMKRIIK
CEEECCEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVSLQFITHQTDRYTYFESALMALEGGCKWIQLRMKEAPCEEVEAVALQLKPLCKEKEAI
CEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEE
LLLDDHVELAKKLEVDGVHLGKKDMPIDQARQLLGEAFIIGGTANTFEDVVQHYRAGADY
EEEECHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCEEEECCCCHHHHHHHHHHHCCCCE
LGIGPFRFTTTKKNLSPVLGLEGYTAILSQMKEANIELPVVAIGGITREDIPAILETGVN
ECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCC
GIALSGTILRAEDPAAETRKILNMKRIIK
CEEECCEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928