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The map label for this gene is dapF

Identifier: 29345958

GI number: 29345958

Start: 674370

End: 675173

Strand: Reverse

Name: dapF

Synonym: BT_0548

Alternate gene names: 29345958

Gene position: 675173-674370 (Counterclockwise)

Preceding gene: 29345960

Following gene: 29345957

Centisome position: 10.78

GC content: 44.65

Gene sequence:

>804_bases
ATGACAACCAAGATTAAGTTCACAAAAATGCATGGAGCAGGTAATGACTACATATATGTAGATACTACCCGATATCCGAT
AGCAGCCCCTGAAAAGAAAGCAATCGAATGGAGCAAGTTTCATACGGGAATTGGAAGTGACGGACTTATATTGATTGGCA
GTTCTGACAAAGCAGATTTCAGTATGCGTATATTTAATGCAGATGGTTCGGAAGCAATGATGTGTGGCAATGGAAGCCGT
TGTGTAGGCAAGTATGTATACGAATACGGATTAACTGCCAAAAAGGAAATCACGCTTGACACCCGTTCGGGCATTAAGGT
TCTGAAATTACATGTAGAAGGAGGAAAGGTGACGGCAGTCACTGTAGATATGGGTAGTCCTCTGGAAACGGAAGCGGTAG
ATTTCGGCGATCAATTTCCTTTTCAGTCTACCCGGGTATCAATGGGAAACCCGCATCTGGTAACTTTTGTAGAAGATATT
ACCCAAATCAATCTGCCGGAAATTGGTCCGCAGTTAGAAAATTATCATCTCTTTCCGGACAGGACAAATGTAGAGTTTGC
TCAGATCGTAGGTAAAGACACCATTCGGATGAGAGTTTGGGAAAGAGGTTCAGGAATCACCCAGGCCTGTGGAACAGGCG
CTTGTGCCACGGCTGTAGCCGCTGTTCTCCATGGATTGGCAGGAAGAAAATGTGATATAATAATGGATGGGGGAACCGTC
ACCATCGAGTGGGAAGAAGCAACGGGACACATATTAATGACAGGACCGGCAACTAAAGTTTTTGATGGGGAGATGGAGGG
ATAG

Upstream 100 bases:

>100_bases
TAGAGATTAGACATCTTTTCATCAAAAGTTAAATCAAAAAGTCATATCTTTGCATTGTAATAAAACAAAAGGATACAACA
ACCAATTAATAAAGAACAAA

Downstream 100 bases:

>100_bases
GTAATAAGTATTAGGTAATAGGTAACAGGTTTTGCAGAAACTTAAAACAATAACAATATGGCATTAGTAAACGAACATTT
TTTGAAATTACCGGGGAGCT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTTKIKFTKMHGAGNDYIYVDTTRYPIAAPEKKAIEWSKFHTGIGSDGLILIGSSDKADFSMRIFNADGSEAMMCGNGSR
CVGKYVYEYGLTAKKEITLDTRSGIKVLKLHVEGGKVTAVTVDMGSPLETEAVDFGDQFPFQSTRVSMGNPHLVTFVEDI
TQINLPEIGPQLENYHLFPDRTNVEFAQIVGKDTIRMRVWERGSGITQACGTGACATAVAAVLHGLAGRKCDIIMDGGTV
TIEWEEATGHILMTGPATKVFDGEMEG

Sequences:

>Translated_267_residues
MTTKIKFTKMHGAGNDYIYVDTTRYPIAAPEKKAIEWSKFHTGIGSDGLILIGSSDKADFSMRIFNADGSEAMMCGNGSR
CVGKYVYEYGLTAKKEITLDTRSGIKVLKLHVEGGKVTAVTVDMGSPLETEAVDFGDQFPFQSTRVSMGNPHLVTFVEDI
TQINLPEIGPQLENYHLFPDRTNVEFAQIVGKDTIRMRVWERGSGITQACGTGACATAVAAVLHGLAGRKCDIIMDGGTV
TIEWEEATGHILMTGPATKVFDGEMEG
>Mature_266_residues
TTKIKFTKMHGAGNDYIYVDTTRYPIAAPEKKAIEWSKFHTGIGSDGLILIGSSDKADFSMRIFNADGSEAMMCGNGSRC
VGKYVYEYGLTAKKEITLDTRSGIKVLKLHVEGGKVTAVTVDMGSPLETEAVDFGDQFPFQSTRVSMGNPHLVTFVEDIT
QINLPEIGPQLENYHLFPDRTNVEFAQIVGKDTIRMRVWERGSGITQACGTGACATAVAAVLHGLAGRKCDIIMDGGTVT
IEWEEATGHILMTGPATKVFDGEMEG

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=280, Percent_Identity=37.1428571428571, Blast_Score=187, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_BACTN (Q8AAB7)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_809461.1
- ProteinModelPortal:   Q8AAB7
- GeneID:   1072044
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_0548
- NMPDR:   fig|226186.1.peg.548
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   Q8AAB7
- ProtClustDB:   PRK00450
- BioCyc:   BTHE226186:BT_0548-MONOMER
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 28970; Mature: 28839

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 210-210

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKIKFTKMHGAGNDYIYVDTTRYPIAAPEKKAIEWSKFHTGIGSDGLILIGSSDKADF
CCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCEEHHHHCCCCCCCCEEEEECCCCCCE
SMRIFNADGSEAMMCGNGSRCVGKYVYEYGLTAKKEITLDTRSGIKVLKLHVEGGKVTAV
EEEEEECCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEECCCEEEEE
TVDMGSPLETEAVDFGDQFPFQSTRVSMGNPHLVTFVEDITQINLPEIGPQLENYHLFPD
EEECCCCCCCCEECCCCCCCCCCCEEECCCCEEEEEEHHHHHCCCCCCCCCCCCEEECCC
RTNVEFAQIVGKDTIRMRVWERGSGITQACGTGACATAVAAVLHGLAGRKCDIIMDGGTV
CCCCHHHHHHCCCEEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCEE
TIEWEEATGHILMTGPATKVFDGEMEG
EEEEECCCCEEEEECCCCEEECCCCCC
>Mature Secondary Structure 
TTKIKFTKMHGAGNDYIYVDTTRYPIAAPEKKAIEWSKFHTGIGSDGLILIGSSDKADF
CCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCEEHHHHCCCCCCCCEEEEECCCCCCE
SMRIFNADGSEAMMCGNGSRCVGKYVYEYGLTAKKEITLDTRSGIKVLKLHVEGGKVTAV
EEEEEECCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEECCCEEEEE
TVDMGSPLETEAVDFGDQFPFQSTRVSMGNPHLVTFVEDITQINLPEIGPQLENYHLFPD
EEECCCCCCCCEECCCCCCCCCCCEEECCCCEEEEEEHHHHHCCCCCCCCCCCCEEECCC
RTNVEFAQIVGKDTIRMRVWERGSGITQACGTGACATAVAAVLHGLAGRKCDIIMDGGTV
CCCCHHHHHHCCCEEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCEE
TIEWEEATGHILMTGPATKVFDGEMEG
EEEEECCCCEEEEECCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928