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The map label for this gene is ansA [H]

Identifier: 29345936

GI number: 29345936

Start: 649703

End: 650743

Strand: Reverse

Name: ansA [H]

Synonym: BT_0526

Alternate gene names: 29345936

Gene position: 650743-649703 (Counterclockwise)

Preceding gene: 29345937

Following gene: 29345935

Centisome position: 10.39

GC content: 43.23

Gene sequence:

>1041_bases
ATGAGAGTAGAGACCCCTTCCGTTTTGTTAATTTACACGGGTGGAACTATCGGAATGATAGAAAATCCGGAGACAGGTGC
CTTAGAAAACTTTAATTTCGACCATTTGCTCAAGCACGTTCCTGAGCTGAAAAGATTCAACTATCGCATTTCCTCCTATC
AATTCGATCCTCCTCTCGACTCCTCGGATATGGAACCAGCTTACTGGGCAAAACTTGTAAAGATCATCAACTATAATTAC
GACTATTTCGATGGCTTTGTAATTTTGCATGGAACGGATACAATGGCCTATACGGCTTCTGCCCTAAGTTTTATGCTCGA
AAATCTGAGTAAGCCTGTTATTCTGACCGGTTCTCAATTGCCAATCGGAACGCTGCGTACGGACGGAAAAGAAAATCTGA
TCACTGCAATCGAGATTGCTGCTGCCAAGAATCCGGACGGTACAGCTATTGTCCCCGAAGTATGTATTTTCTTTGAAAAT
CACTTGATGCGGGGTAATCGTACCACAAAAATCAATGCTGAGAACTTTAATGCCTTCCGGTCTTTCAATTACCCGCCATT
GGCACGCGTAGGCATCCATATTAAATACGAGCCCAACCTTATTCGCAAACCGGACCCGGACAAACCACTAAAGCCACACT
ATCTGTTTGATACGAATGTGGTAATATTAACTCTTTTCCCCGGCATCCAGGAGGAGATCATTCATTCACTACTCCATGTC
CCCGGATTGAAAGCGGTCGTCATGAAAACATTCGGATCAGGAAATGCACCGCAAAAAGAATGGTTCATCCGGGAATTAAA
AGAGGCTACTGATCGTGGAATTATCATTGTTAACATCACACAATGTGCTTCCGGAGCAGTAGAAATGGGACGTTACGAGA
CCGGAATGCATCTACTCGAAGCAGGTGTTATCAGTGGATATGACAGTACTCCCGAATGTGCTGTAACTAAACTGATGTTT
TTGCTGGGACACGGACTATCTAATAAAGACATCCGATACAAAATGAACTCTTGTTTAATAGGGGAAATTACCAAACCCTA
A

Upstream 100 bases:

>100_bases
TAACATTTCACTGCAAACTACGAACAAATTCATCATTTACTATTCATAATTCGCAATTAATCTATATCTTTGTCAGCGTT
TTAACAGAAATTCATCAATT

Downstream 100 bases:

>100_bases
AAAAAAATCTCTATAGTAATCTCCTGATTTTCATTCTATAATATTTCTGCAAAAGAAAAAATCAGGAGGTTTTTCTTTCG
TTTTTCATATAAAGCGAACC

Product: L-asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MRVETPSVLLIYTGGTIGMIENPETGALENFNFDHLLKHVPELKRFNYRISSYQFDPPLDSSDMEPAYWAKLVKIINYNY
DYFDGFVILHGTDTMAYTASALSFMLENLSKPVILTGSQLPIGTLRTDGKENLITAIEIAAAKNPDGTAIVPEVCIFFEN
HLMRGNRTTKINAENFNAFRSFNYPPLARVGIHIKYEPNLIRKPDPDKPLKPHYLFDTNVVILTLFPGIQEEIIHSLLHV
PGLKAVVMKTFGSGNAPQKEWFIRELKEATDRGIIIVNITQCASGAVEMGRYETGMHLLEAGVISGYDSTPECAVTKLMF
LLGHGLSNKDIRYKMNSCLIGEITKP

Sequences:

>Translated_346_residues
MRVETPSVLLIYTGGTIGMIENPETGALENFNFDHLLKHVPELKRFNYRISSYQFDPPLDSSDMEPAYWAKLVKIINYNY
DYFDGFVILHGTDTMAYTASALSFMLENLSKPVILTGSQLPIGTLRTDGKENLITAIEIAAAKNPDGTAIVPEVCIFFEN
HLMRGNRTTKINAENFNAFRSFNYPPLARVGIHIKYEPNLIRKPDPDKPLKPHYLFDTNVVILTLFPGIQEEIIHSLLHV
PGLKAVVMKTFGSGNAPQKEWFIRELKEATDRGIIIVNITQCASGAVEMGRYETGMHLLEAGVISGYDSTPECAVTKLMF
LLGHGLSNKDIRYKMNSCLIGEITKP
>Mature_346_residues
MRVETPSVLLIYTGGTIGMIENPETGALENFNFDHLLKHVPELKRFNYRISSYQFDPPLDSSDMEPAYWAKLVKIINYNY
DYFDGFVILHGTDTMAYTASALSFMLENLSKPVILTGSQLPIGTLRTDGKENLITAIEIAAAKNPDGTAIVPEVCIFFEN
HLMRGNRTTKINAENFNAFRSFNYPPLARVGIHIKYEPNLIRKPDPDKPLKPHYLFDTNVVILTLFPGIQEEIIHSLLHV
PGLKAVVMKTFGSGNAPQKEWFIRELKEATDRGIIIVNITQCASGAVEMGRYETGMHLLEAGVISGYDSTPECAVTKLMF
LLGHGLSNKDIRYKMNSCLIGEITKP

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=362, Percent_Identity=44.1988950276243, Blast_Score=256, Evalue=2e-68,
Organism=Escherichia coli, GI1788065, Length=343, Percent_Identity=45.4810495626822, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1789327, Length=254, Percent_Identity=28.740157480315, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17558280, Length=369, Percent_Identity=39.5663956639566, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17558278, Length=369, Percent_Identity=39.5663956639566, Blast_Score=234, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI193207372, Length=369, Percent_Identity=39.5663956639566, Blast_Score=234, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI193207374, Length=369, Percent_Identity=39.5663956639566, Blast_Score=234, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6323189, Length=303, Percent_Identity=27.0627062706271, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323187, Length=303, Percent_Identity=27.0627062706271, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323186, Length=303, Percent_Identity=27.0627062706271, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323184, Length=303, Percent_Identity=27.0627062706271, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320527, Length=205, Percent_Identity=25.8536585365854, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24639609, Length=373, Percent_Identity=43.4316353887399, Blast_Score=267, Evalue=8e-72,
Organism=Drosophila melanogaster, GI24645530, Length=340, Percent_Identity=42.0588235294118, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 38732; Mature: 38732

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVETPSVLLIYTGGTIGMIENPETGALENFNFDHLLKHVPELKRFNYRISSYQFDPPLD
CCCCCCCEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCEEECCEECCCCCC
SSDMEPAYWAKLVKIINYNYDYFDGFVILHGTDTMAYTASALSFMLENLSKPVILTGSQL
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCC
PIGTLRTDGKENLITAIEIAAAKNPDGTAIVPEVCIFFENHLMRGNRTTKINAENFNAFR
CCCEECCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
SFNYPPLARVGIHIKYEPNLIRKPDPDKPLKPHYLFDTNVVILTLFPGIQEEIIHSLLHV
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCEEEEEECCCHHHHHHHHHHHC
PGLKAVVMKTFGSGNAPQKEWFIRELKEATDRGIIIVNITQCASGAVEMGRYETGMHLLE
CCHHEEHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCHHHHHHHHH
AGVISGYDSTPECAVTKLMFLLGHGLSNKDIRYKMNSCLIGEITKP
HHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEECCCC
>Mature Secondary Structure
MRVETPSVLLIYTGGTIGMIENPETGALENFNFDHLLKHVPELKRFNYRISSYQFDPPLD
CCCCCCCEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCEEECCEECCCCCC
SSDMEPAYWAKLVKIINYNYDYFDGFVILHGTDTMAYTASALSFMLENLSKPVILTGSQL
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCC
PIGTLRTDGKENLITAIEIAAAKNPDGTAIVPEVCIFFENHLMRGNRTTKINAENFNAFR
CCCEECCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
SFNYPPLARVGIHIKYEPNLIRKPDPDKPLKPHYLFDTNVVILTLFPGIQEEIIHSLLHV
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCEEEEEECCCHHHHHHHHHHHC
PGLKAVVMKTFGSGNAPQKEWFIRELKEATDRGIIIVNITQCASGAVEMGRYETGMHLLE
CCHHEEHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCHHHHHHHHH
AGVISGYDSTPECAVTKLMFLLGHGLSNKDIRYKMNSCLIGEITKP
HHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]