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The map label for this gene is trpA

Identifier: 29345937

GI number: 29345937

Start: 651009

End: 651779

Strand: Reverse

Name: trpA

Synonym: BT_0527

Alternate gene names: 29345937

Gene position: 651779-651009 (Counterclockwise)

Preceding gene: 29345938

Following gene: 29345936

Centisome position: 10.41

GC content: 43.06

Gene sequence:

>771_bases
ATGAACAGAATTAATCAACTCTTTAATAGCAACAAGAAAGACATCCTTTCTATTTATTTTTGTGCAGGTAATCCTACCCT
TGATGGAACAGTCAATGTCATCCGTACTCTCGAAAAACATGGCGTCAGCATGATTGAAGTCGGTATTCCATTCAGTGACC
CTATGGCAGACGGCATCGTTATCCAGAATGCCGCCACTCAGGCATTACGCAACGGAATGTCATTGAAAATCCTTTTCGAA
CAATTACGAAACATTCGCCAGGAAGTAAGTATTCCATTGGTATTTATGGGATACTTAAACCCTATTATGCAGTTCGGATT
TGAAAACTTCTGCCGCAAATGCGTGGAATGCGGAATAGACGGCGTTATCATCCCCGACTTGCCTTTCAGAGATTATCAGG
ATCACTACCGTATCATTGCCGAACGTTATGGAATCAAAGTAATCATGCTCATCACCCCGGAGACCAGCGAAGAGCGGGTA
CGTGAGATCGATGCACATACAGACGGATTCATCTATATGGTTTCATCCGCAGCAACTACAGGCGCACAACAAGACTTTAA
TGAACAGAAACGTGCCTATTTCAAAAAGATCGAAGATATGAATCTGCGCAACCCGCTAATGGTTGGTTTCGGTATCTCCA
ACAAAGCTACTTTTCAAGCAGCTTGCGAACATGCTTCCGGAGCTATCATCGGTAGCCGCTTTGTCACTCTGCTTGAAGAA
GAAAAAGATCCGGAAAAAGCCATTCTAAAACTGAAAGATGCGCTTAAATAG

Upstream 100 bases:

>100_bases
CCTGAACAGTCGTTTCGAACTGAAGCCCGGGGAAAAAGATCCGGAACGAATCCGGATATTTCTAAATGAACTAAAGTCAT
AAAATTAAATTCTCAAAATC

Downstream 100 bases:

>100_bases
ACATAAGAAATAGTCAGACAAATGGTAGCTAAAATAGCCGGGCAGTATCGTTTTGCTTTGCATAACCATTCTTTCCAAAC
AATTTTAGCTACTTGACTAT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MNRINQLFNSNKKDILSIYFCAGNPTLDGTVNVIRTLEKHGVSMIEVGIPFSDPMADGIVIQNAATQALRNGMSLKILFE
QLRNIRQEVSIPLVFMGYLNPIMQFGFENFCRKCVECGIDGVIIPDLPFRDYQDHYRIIAERYGIKVIMLITPETSEERV
REIDAHTDGFIYMVSSAATTGAQQDFNEQKRAYFKKIEDMNLRNPLMVGFGISNKATFQAACEHASGAIIGSRFVTLLEE
EKDPEKAILKLKDALK

Sequences:

>Translated_256_residues
MNRINQLFNSNKKDILSIYFCAGNPTLDGTVNVIRTLEKHGVSMIEVGIPFSDPMADGIVIQNAATQALRNGMSLKILFE
QLRNIRQEVSIPLVFMGYLNPIMQFGFENFCRKCVECGIDGVIIPDLPFRDYQDHYRIIAERYGIKVIMLITPETSEERV
REIDAHTDGFIYMVSSAATTGAQQDFNEQKRAYFKKIEDMNLRNPLMVGFGISNKATFQAACEHASGAIIGSRFVTLLEE
EKDPEKAILKLKDALK
>Mature_256_residues
MNRINQLFNSNKKDILSIYFCAGNPTLDGTVNVIRTLEKHGVSMIEVGIPFSDPMADGIVIQNAATQALRNGMSLKILFE
QLRNIRQEVSIPLVFMGYLNPIMQFGFENFCRKCVECGIDGVIIPDLPFRDYQDHYRIIAERYGIKVIMLITPETSEERV
REIDAHTDGFIYMVSSAATTGAQQDFNEQKRAYFKKIEDMNLRNPLMVGFGISNKATFQAACEHASGAIIGSRFVTLLEE
EKDPEKAILKLKDALK

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=258, Percent_Identity=31.3953488372093, Blast_Score=121, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321412, Length=239, Percent_Identity=36.4016736401674, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_BACTN (Q8AAD8)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_809440.1
- ProteinModelPortal:   Q8AAD8
- SMR:   Q8AAD8
- GeneID:   1071997
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_0527
- NMPDR:   fig|226186.1.peg.527
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   BTHE226186:BT_0527-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28881; Mature: 28881

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 46-46 ACT_SITE 57-57

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRINQLFNSNKKDILSIYFCAGNPTLDGTVNVIRTLEKHGVSMIEVGIPFSDPMADGIV
CCHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEE
IQNAATQALRNGMSLKILFEQLRNIRQEVSIPLVFMGYLNPIMQFGFENFCRKCVECGID
EEHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCC
GVIIPDLPFRDYQDHYRIIAERYGIKVIMLITPETSEERVREIDAHTDGFIYMVSSAATT
CEEECCCCCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCC
GAQQDFNEQKRAYFKKIEDMNLRNPLMVGFGISNKATFQAACEHASGAIIGSRFVTLLEE
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHC
EKDPEKAILKLKDALK
CCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNRINQLFNSNKKDILSIYFCAGNPTLDGTVNVIRTLEKHGVSMIEVGIPFSDPMADGIV
CCHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEE
IQNAATQALRNGMSLKILFEQLRNIRQEVSIPLVFMGYLNPIMQFGFENFCRKCVECGID
EEHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCC
GVIIPDLPFRDYQDHYRIIAERYGIKVIMLITPETSEERVREIDAHTDGFIYMVSSAATT
CEEECCCCCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCC
GAQQDFNEQKRAYFKKIEDMNLRNPLMVGFGISNKATFQAACEHASGAIIGSRFVTLLEE
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHC
EKDPEKAILKLKDALK
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928