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The map label for this gene is malS [H]

Identifier: 29144133

GI number: 29144133

Start: 3981034

End: 3983061

Strand: Reverse

Name: malS [H]

Synonym: t3855

Alternate gene names: 29144133

Gene position: 3983061-3981034 (Counterclockwise)

Preceding gene: 29144135

Following gene: 29144132

Centisome position: 83.12

GC content: 55.57

Gene sequence:

>2028_bases
ATGAAACTTGCCGCCTTCGCTCTGACGCTGATACCCGGTATCGCGATCGCCTCATCATGGACCTCGCCCGGTTTTCCGAC
GCTCTCGACGCAGGAAACCGGACGCTTTACCAGCCATGCTGCATTAACAAAAGGTACACGCGCGTTAACGCTCCATATTG
ACCAGCAGTGCTGGCAGCCCTCCGGCGCTATAAAACTCAACCAAATGTTGTCGTTAAAACCCTGCGAAGGCGCGCCGCCG
CAATGGCGTCTGTTTAAAGATGGCGACTATACGATAACGGTAGATACCCGATCCGGTACGCCGACCCTGTTGTTATCGAT
AAAAACTGAACCTGAACGCACCACGCAGCTCGCCTACCAGTGTCCCGTCTGGGATGGTTCGCCGCTCACGCTGGATGTTC
GCCAAACCTTTCCGGAAGGGACGGTAGTGCGCGATTATTACAGTGGTCAAATCGATACCGTCCAAAACGGAAAAATCACG
CTGCTACCTGCCGACAGCCACGGGCTTTTATTGCTGGAACGCGCGGAAACCCATGCGTCAGCGCCTTTTAACTGGCGCAA
CGCCACCGTTTATTTTGTGCTTACGGATCGCTTTCGCAATGGCGATCCAACCAACGACCACAGTTATGGTCGCCATAAGG
ATGGTATGCAAGAGATTGGCACTTTCCACGGCGGCGATTTGCGCGGGTTGACGAGTCAACTGGACTATCTACAGCAATTA
GGCGTGAACGCCTTGTGGATAAGCTCGCCGTTTGAACAGATCCACGGCTGGGTCGGCGGCGGAACAAAAGGCGATTTTCC
TCATTACGCCTATCACGGCTATTACACTCAGGACTGGACGACGCTGGATGCCAATATGGGCAGCGAAGCCGATCTCCGCG
CGCTGGTCGACGGCGCGCACCAGCGCGGCATCCGTATTTTATTTGACGTAGTAATGAATCATGCCGGTTACGCCACGCTG
GCGGATATGCAGGAGTATCAGTTCGGCGCGCTCTATTTATCCGGCGCGGAACGGCAAAAAATTCTCGGCGATCGCTGGAC
AAACTGGCGACCGGCCGCCGGACAAAGCTGGCACAGCTTTAACGACTACATCAACTTCAGCGACAGCGCCGCCTGGGAAA
AATGGTGGGGGAAAAAGTGGATTCGTACCGATATTGGCGACTACGACAGTCCGGGATTTGACGATTTAACCCTGTCGCTG
GCCTTCCTGCCGGATATAAAAACGGAATCTACCACGCCTTCCGGCCTACCCGTGTTCTATGCCAACAAACCCGATACTAA
AGCAAAGTTCATTGAGGGCTATACGCCACGGGATTATCTGACTCACTGGTTAAGCCAGTGGGTGCATGATTACGGCATTG
ACGGGTTCCGGATCGATACTGCCAAAAACGTTGAGCTTCCTGCCTGGCAACAGCTAAAAACCCAGGCCAGCGCGGCGTTA
CATGAATGGAAGCAGGCCAATCCGGACAAAGCGCTGGATGATAGCCCGTTCTGGATGACTGGCGAAGCGTGGGGCCACGG
CGTCATGAAAAGTGATTATTATCGCTATGGTTTCGACGCGATGATCAATTTTGATTATCAGGAGCAGGCGGCGAAAGCGG
TCGATTGCCTGGCGGAAATGGGGCCAGTCTGGCAGCAGATGGCGGATAAAATGCAGGATTTCAACGTATTAAGTTACCTC
TCCTCGCATGATACGCGCCTTTTCCGTGAGGGCGGTGATAAGGCGGCGGAACTGCTGCTGCTTTCGCCAGGCGCGGTGCA
GATCTTTTACGGCGATGAATCCGCTCGTCCCTTCGGCCCCACCGGCTCCGACCCGCTGCAAGGCACCCGTTCTGATATGA
ACTGGCAGGACGTGAGCGGAAAGTCAGCCGCAGCCGTCGCGCACTGGCAGCGTATTAGCCAGTTCCGCGCCAGACATCCC
GCCATCGGCGCAGGCCAACAAACCACGCTGACGCTAAAACACGGGTACGGTTTTGTACGCCAGTACGGCGACGATACGGT
GATGGTCGTCTGGGCGGGCCGCCGCTAA

Upstream 100 bases:

>100_bases
TCACCCTCGCGCCTCCTCCTGCGCGACTCTCTGCTGGGGAGGAGTTCATTCGCCTAAAAACCAGGCAAGCTGATGAATAT
TGCCCACAAAGGATAGCGTG

Downstream 100 bases:

>100_bases
TCGTGGCGCTACCGGTCCCCTCGCGTACCGGTAACCTCTTCAAAATAAAAAACTAAACTGATAAAAATTTGTAGCATAAA
ACATCAATACCACCGCCTGT

Product: periplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 675; Mature: 675

Protein sequence:

>675_residues
MKLAAFALTLIPGIAIASSWTSPGFPTLSTQETGRFTSHAALTKGTRALTLHIDQQCWQPSGAIKLNQMLSLKPCEGAPP
QWRLFKDGDYTITVDTRSGTPTLLLSIKTEPERTTQLAYQCPVWDGSPLTLDVRQTFPEGTVVRDYYSGQIDTVQNGKIT
LLPADSHGLLLLERAETHASAPFNWRNATVYFVLTDRFRNGDPTNDHSYGRHKDGMQEIGTFHGGDLRGLTSQLDYLQQL
GVNALWISSPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTTLDANMGSEADLRALVDGAHQRGIRILFDVVMNHAGYATL
ADMQEYQFGALYLSGAERQKILGDRWTNWRPAAGQSWHSFNDYINFSDSAAWEKWWGKKWIRTDIGDYDSPGFDDLTLSL
AFLPDIKTESTTPSGLPVFYANKPDTKAKFIEGYTPRDYLTHWLSQWVHDYGIDGFRIDTAKNVELPAWQQLKTQASAAL
HEWKQANPDKALDDSPFWMTGEAWGHGVMKSDYYRYGFDAMINFDYQEQAAKAVDCLAEMGPVWQQMADKMQDFNVLSYL
SSHDTRLFREGGDKAAELLLLSPGAVQIFYGDESARPFGPTGSDPLQGTRSDMNWQDVSGKSAAAVAHWQRISQFRARHP
AIGAGQQTTLTLKHGYGFVRQYGDDTVMVVWAGRR

Sequences:

>Translated_675_residues
MKLAAFALTLIPGIAIASSWTSPGFPTLSTQETGRFTSHAALTKGTRALTLHIDQQCWQPSGAIKLNQMLSLKPCEGAPP
QWRLFKDGDYTITVDTRSGTPTLLLSIKTEPERTTQLAYQCPVWDGSPLTLDVRQTFPEGTVVRDYYSGQIDTVQNGKIT
LLPADSHGLLLLERAETHASAPFNWRNATVYFVLTDRFRNGDPTNDHSYGRHKDGMQEIGTFHGGDLRGLTSQLDYLQQL
GVNALWISSPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTTLDANMGSEADLRALVDGAHQRGIRILFDVVMNHAGYATL
ADMQEYQFGALYLSGAERQKILGDRWTNWRPAAGQSWHSFNDYINFSDSAAWEKWWGKKWIRTDIGDYDSPGFDDLTLSL
AFLPDIKTESTTPSGLPVFYANKPDTKAKFIEGYTPRDYLTHWLSQWVHDYGIDGFRIDTAKNVELPAWQQLKTQASAAL
HEWKQANPDKALDDSPFWMTGEAWGHGVMKSDYYRYGFDAMINFDYQEQAAKAVDCLAEMGPVWQQMADKMQDFNVLSYL
SSHDTRLFREGGDKAAELLLLSPGAVQIFYGDESARPFGPTGSDPLQGTRSDMNWQDVSGKSAAAVAHWQRISQFRARHP
AIGAGQQTTLTLKHGYGFVRQYGDDTVMVVWAGRR
>Mature_675_residues
MKLAAFALTLIPGIAIASSWTSPGFPTLSTQETGRFTSHAALTKGTRALTLHIDQQCWQPSGAIKLNQMLSLKPCEGAPP
QWRLFKDGDYTITVDTRSGTPTLLLSIKTEPERTTQLAYQCPVWDGSPLTLDVRQTFPEGTVVRDYYSGQIDTVQNGKIT
LLPADSHGLLLLERAETHASAPFNWRNATVYFVLTDRFRNGDPTNDHSYGRHKDGMQEIGTFHGGDLRGLTSQLDYLQQL
GVNALWISSPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTTLDANMGSEADLRALVDGAHQRGIRILFDVVMNHAGYATL
ADMQEYQFGALYLSGAERQKILGDRWTNWRPAAGQSWHSFNDYINFSDSAAWEKWWGKKWIRTDIGDYDSPGFDDLTLSL
AFLPDIKTESTTPSGLPVFYANKPDTKAKFIEGYTPRDYLTHWLSQWVHDYGIDGFRIDTAKNVELPAWQQLKTQASAAL
HEWKQANPDKALDDSPFWMTGEAWGHGVMKSDYYRYGFDAMINFDYQEQAAKAVDCLAEMGPVWQQMADKMQDFNVLSYL
SSHDTRLFREGGDKAAELLLLSPGAVQIFYGDESARPFGPTGSDPLQGTRSDMNWQDVSGKSAAAVAHWQRISQFRARHP
AIGAGQQTTLTLKHGYGFVRQYGDDTVMVVWAGRR

Specific function: Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=676, Percent_Identity=81.508875739645, Blast_Score=1165, Evalue=0.0,
Organism=Escherichia coli, GI1786604, Length=143, Percent_Identity=30.0699300699301, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014635
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 75689; Mature: 75689

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAAFALTLIPGIAIASSWTSPGFPTLSTQETGRFTSHAALTKGTRALTLHIDQQCWQP
CCHHHHHHHHHCCHHEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEEECHHHCCC
SGAIKLNQMLSLKPCEGAPPQWRLFKDGDYTITVDTRSGTPTLLLSIKTEPERTTQLAYQ
CCCEEEEEEEEECCCCCCCCCEEEEECCCEEEEEECCCCCCEEEEEEECCCCCHHEEEEE
CPVWDGSPLTLDVRQTFPEGTVVRDYYSGQIDTVQNGKITLLPADSHGLLLLERAETHAS
CCCCCCCCEEEEHHHCCCCCCEEEEECCCEEEEECCCEEEEEECCCCCEEEEEECCCCCC
APFNWRNATVYFVLTDRFRNGDPTNDHSYGRHKDGMQEIGTFHGGDLRGLTSQLDYLQQL
CCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHC
GVNALWISSPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTTLDANMGSEADLRALVDGAH
CCCEEEECCCHHHHCCCCCCCCCCCCCCEEECCEEECCCEEEECCCCCCHHHHHHHHHHH
QRGIRILFDVVMNHAGYATLADMQEYQFGALYLSGAERQKILGDRWTNWRPAAGQSWHSF
HCCHHHHHHHHHCCCCCEEHHHHHHCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCH
NDYINFSDSAAWEKWWGKKWIRTDIGDYDSPGFDDLTLSLAFLPDIKTESTTPSGLPVFY
HCEECCCCCHHHHHHHCCCHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEE
ANKPDTKAKFIEGYTPRDYLTHWLSQWVHDYGIDGFRIDTAKNVELPAWQQLKTQASAAL
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHH
HEWKQANPDKALDDSPFWMTGEAWGHGVMKSDYYRYGFDAMINFDYQEQAAKAVDCLAEM
HHHHHCCCCCCCCCCCCEEECHHHCCCCCCCCCEEECCEEEEECCHHHHHHHHHHHHHHH
GPVWQQMADKMQDFNVLSYLSSHDTRLFREGGDKAAELLLLSPGAVQIFYGDESARPFGP
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEEEECCCEEEEEECCCCCCCCCC
TGSDPLQGTRSDMNWQDVSGKSAAAVAHWQRISQFRARHPAIGAGQQTTLTLKHGYGFVR
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHH
QYGDDTVMVVWAGRR
HCCCCEEEEEECCCC
>Mature Secondary Structure
MKLAAFALTLIPGIAIASSWTSPGFPTLSTQETGRFTSHAALTKGTRALTLHIDQQCWQP
CCHHHHHHHHHCCHHEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEEECHHHCCC
SGAIKLNQMLSLKPCEGAPPQWRLFKDGDYTITVDTRSGTPTLLLSIKTEPERTTQLAYQ
CCCEEEEEEEEECCCCCCCCCEEEEECCCEEEEEECCCCCCEEEEEEECCCCCHHEEEEE
CPVWDGSPLTLDVRQTFPEGTVVRDYYSGQIDTVQNGKITLLPADSHGLLLLERAETHAS
CCCCCCCCEEEEHHHCCCCCCEEEEECCCEEEEECCCEEEEEECCCCCEEEEEECCCCCC
APFNWRNATVYFVLTDRFRNGDPTNDHSYGRHKDGMQEIGTFHGGDLRGLTSQLDYLQQL
CCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHC
GVNALWISSPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTTLDANMGSEADLRALVDGAH
CCCEEEECCCHHHHCCCCCCCCCCCCCCEEECCEEECCCEEEECCCCCCHHHHHHHHHHH
QRGIRILFDVVMNHAGYATLADMQEYQFGALYLSGAERQKILGDRWTNWRPAAGQSWHSF
HCCHHHHHHHHHCCCCCEEHHHHHHCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCH
NDYINFSDSAAWEKWWGKKWIRTDIGDYDSPGFDDLTLSLAFLPDIKTESTTPSGLPVFY
HCEECCCCCHHHHHHHCCCHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEE
ANKPDTKAKFIEGYTPRDYLTHWLSQWVHDYGIDGFRIDTAKNVELPAWQQLKTQASAAL
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHH
HEWKQANPDKALDDSPFWMTGEAWGHGVMKSDYYRYGFDAMINFDYQEQAAKAVDCLAEM
HHHHHCCCCCCCCCCCCEEECHHHCCCCCCCCCEEECCEEEEECCHHHHHHHHHHHHHHH
GPVWQQMADKMQDFNVLSYLSSHDTRLFREGGDKAAELLLLSPGAVQIFYGDESARPFGP
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEEEECCCEEEEEECCCCCCCCCC
TGSDPLQGTRSDMNWQDVSGKSAAAVAHWQRISQFRARHPAIGAGQQTTLTLKHGYGFVR
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHH
QYGDDTVMVVWAGRR
HCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1544897; 8041620; 9278503; 9268356 [H]