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The map label for this gene is emrD [H]

Identifier: 29144011

GI number: 29144011

Start: 3833345

End: 3834529

Strand: Reverse

Name: emrD [H]

Synonym: t3719

Alternate gene names: 29144011

Gene position: 3834529-3833345 (Counterclockwise)

Preceding gene: 29144015

Following gene: 29144008

Centisome position: 80.02

GC content: 58.31

Gene sequence:

>1185_bases
ATGAAAAGGCAGAGAAACGTCAATTTGTTGTTGATGTTGGTGTTACTGGTGGCCGTAGGGCAGATGGCGCAAACCATTTA
TATTCCCGCCATCGCCGATATGGCACAAGCGCTAAACGTCCGGGAAGGCGCCGTCCAGAGCGTAATGGCTGCTTACCTCC
TGACCTACGGCGTCTCGCAACTGTTTTACGGCCCGCTTTCCGACCGGGTTGGGCGCCGCCCGGTAATCCTCGTCGGCATG
TCTATTTTTATGGTAGCGACTCTGATCGCCATGACCACGCATAGTTTGACGGTGTTGATTGCCGCCAGCGCCATGCAAGG
GATGGGAACCGGCGTTGGCGGAGTAATGGCGAGAACGCTCCCGCGCGATCTGTATGAAGGAACGCAACTTCGTCATGCCA
ATAGCCTGTTAAATATGGGGATTCTGGTCAGCCCGCTGTTAGCGCCGCTGATTGGCGGCCTGCTGGATACCCTGTGGAAC
TGGCGCGCGTGTTACGCTTTCCTGCTGGTGCTTTGCGCTGGCGTCACCTTCAGCATGGCGCGCTGGATGCCGGAAACCCG
CCCCGCCGGCGCGCCGCGTACGCGGCTGATCGCCAGCTATAAGACGCTGTTTGGCAACGGCGCATTTAACTGTTACCTGC
TGATGCTAATCGGCGGGCTGGCTGGCATTGCGGTCTTTGAAGCCTGTTCCGGCGTGCTGATGGGAGCAGTATTAGGTCTC
AGCAGTATGGTGGTAAGCATTCTGTTTATTCTGCCGATTCCGGCGGCGTTCTTCGGCGCCTGGTTTGCCGGACGCCCGAA
TAAACGCTTCTCAGCCCTGATGTGGCAGTCAGTTATTTGCTGTCTGCTGGCAGGCCTTATGATGTGGATTCCCGGCTGGT
TTGGCGTGATGAACGTCTGGACGCTACTCATCCCCGCTGCGCTGTTTTTCTTCGGCGCCGGGATGTTATTTCCACTGGCC
ACCAGCGGCGCGATGGAGCCGTTTCCGTTCCTCGCAGGCACCGCTGGCGCGCTGGTCGGCGGGCTGCAAAATATTGGTTC
CGGCGTACTGGCGTGGCTTTCGGCAATGCTGCCGCAAACCGGTCAGGGCAGTCTGGGATTGCTGATGACCCTTATGGGAT
TGCTGATCTTGGCGTGCTGGCTGCCGCTGGCGTCACGGATATCGCATCAGGGACAGACGGTTTAA

Upstream 100 bases:

>100_bases
TCTGGCCTTCTTTTTCTGGTGTGATAGATTCCTCTCGCATTTATGGGAATGCGCAGCAAGTTATTCTTATTTTTTCTTTT
TTTGAAAAGTAATCAGCGAA

Downstream 100 bases:

>100_bases
CATTCCCCTCGCCCGGCTTTTTGCCGGGCGTTTCACATTCCGGCGCGATCATCGCATTGAGCAGGGGCTCCTGCGCCGGA
CGCCACGCTCCTTCTTCGCC

Product: multidrug resistance protein D

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMAQALNVREGAVQSVMAAYLLTYGVSQLFYGPLSDRVGRRPVILVGM
SIFMVATLIAMTTHSLTVLIAASAMQGMGTGVGGVMARTLPRDLYEGTQLRHANSLLNMGILVSPLLAPLIGGLLDTLWN
WRACYAFLLVLCAGVTFSMARWMPETRPAGAPRTRLIASYKTLFGNGAFNCYLLMLIGGLAGIAVFEACSGVLMGAVLGL
SSMVVSILFILPIPAAFFGAWFAGRPNKRFSALMWQSVICCLLAGLMMWIPGWFGVMNVWTLLIPAALFFFGAGMLFPLA
TSGAMEPFPFLAGTAGALVGGLQNIGSGVLAWLSAMLPQTGQGSLGLLMTLMGLLILACWLPLASRISHQGQTV

Sequences:

>Translated_394_residues
MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMAQALNVREGAVQSVMAAYLLTYGVSQLFYGPLSDRVGRRPVILVGM
SIFMVATLIAMTTHSLTVLIAASAMQGMGTGVGGVMARTLPRDLYEGTQLRHANSLLNMGILVSPLLAPLIGGLLDTLWN
WRACYAFLLVLCAGVTFSMARWMPETRPAGAPRTRLIASYKTLFGNGAFNCYLLMLIGGLAGIAVFEACSGVLMGAVLGL
SSMVVSILFILPIPAAFFGAWFAGRPNKRFSALMWQSVICCLLAGLMMWIPGWFGVMNVWTLLIPAALFFFGAGMLFPLA
TSGAMEPFPFLAGTAGALVGGLQNIGSGVLAWLSAMLPQTGQGSLGLLMTLMGLLILACWLPLASRISHQGQTV
>Mature_394_residues
MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMAQALNVREGAVQSVMAAYLLTYGVSQLFYGPLSDRVGRRPVILVGM
SIFMVATLIAMTTHSLTVLIAASAMQGMGTGVGGVMARTLPRDLYEGTQLRHANSLLNMGILVSPLLAPLIGGLLDTLWN
WRACYAFLLVLCAGVTFSMARWMPETRPAGAPRTRLIASYKTLFGNGAFNCYLLMLIGGLAGIAVFEACSGVLMGAVLGL
SSMVVSILFILPIPAAFFGAWFAGRPNKRFSALMWQSVICCLLAGLMMWIPGWFGVMNVWTLLIPAALFFFGAGMLFPLA
TSGAMEPFPFLAGTAGALVGGLQNIGSGVLAWLSAMLPQTGQGSLGLLMTLMGLLILACWLPLASRISHQGQTV

Specific function: Multidrug resistance pump that participates in a low energy shock adaptative response [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI87082312, Length=394, Percent_Identity=92.6395939086294, Blast_Score=654, Evalue=0.0,
Organism=Escherichia coli, GI48994889, Length=360, Percent_Identity=25, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788509, Length=361, Percent_Identity=24.6537396121884, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790146, Length=347, Percent_Identity=25.9365994236311, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790794, Length=184, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787065, Length=168, Percent_Identity=29.1666666666667, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=170, Percent_Identity=31.1764705882353, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41962; Mature: 41962

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
6.6 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8240355; 7686882; 9278503 [H]