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The map label for this gene is rstA [H]

Identifier: 29141800

GI number: 29141800

Start: 1405300

End: 1406031

Strand: Reverse

Name: rstA [H]

Synonym: t1343

Alternate gene names: 29141800

Gene position: 1406031-1405300 (Counterclockwise)

Preceding gene: 29141802

Following gene: 29141798

Centisome position: 29.34

GC content: 53.01

Gene sequence:

>732_bases
ATGAACCGCATTGTATTTGTTGAAGACGACGCTGAAGTGGGTTCTCTCATTGCCGCTTATCTGGCGAAACATGATATTGA
TGTCATTGTTGAGCCGCGTGGTGACCGGGCGGAGGATCTTATCCTCATCACTCAGCCCGATCTGGTTTTACTGGATATTA
TGCTTCCAGGTAAAGATGGCATGACGATATGCCGCGATTTACGCCACCGCTGGCAAGGTCCGATTGTCCTGCTCACCTCG
CTGGACAGTGATATGAATCATATTCTGGCGCTGGAGATGGGCGCCTGCGACTACATCCTCAAAACCACGCCTCCGGCCGT
TTTGCTGGCGCGTCTGCGCTTACACCTGCGGCAAAGCGAACAAACGCAACAGGCAAAAAGTCTTCAGGAGAGCGCGCTTA
CGCCGCATAAAGCGCTGCGTTTTGGCGCGTTAACCATCGATCCCCTTAACCGGGCGGTCCAGCTCAATGGCGATTTCATA
TCGCTCTCCACCGCCGATTTTGAATTGCTCTGGGAACTGGCGACACACGCCGGACAAATTATGGATCGCGACGCGTTGCT
AAAAACGCTGCGCGGCGTTAATTATGATGGTCTGGATCGCAGCGTGGATGTGGCGATTTCCCGCCTGCGTAAGAAACTGC
TGGATAGCGCTGCTGAGCCCTATCGTATAAAGACCATTCGCAATAAAGGCTATCTTTTTGCCCCTCATGCCTGGGACGAA
ACGACGGGATAA

Upstream 100 bases:

>100_bases
ACAGCATGTAGCATAACGGAACCGCTCTCGTTTAGAAAAGATTTATGGAAGGGAAAACAGAGGAGGTGTATGTTGGCGTT
TTCTATTCTCCATTTATAAT

Downstream 100 bases:

>100_bases
GTGAGTCGACAGATTTTACCTGCCTTTATCAAGTCATAGTGGCGATGCTCATCACCGCCACTGTTCAAAAAACAGACACC
GTATACATCAGTGCTATTTT

Product: DNA-binding transcriptional regulator RstA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MNRIVFVEDDAEVGSLIAAYLAKHDIDVIVEPRGDRAEDLILITQPDLVLLDIMLPGKDGMTICRDLRHRWQGPIVLLTS
LDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQSEQTQQAKSLQESALTPHKALRFGALTIDPLNRAVQLNGDFI
SLSTADFELLWELATHAGQIMDRDALLKTLRGVNYDGLDRSVDVAISRLRKKLLDSAAEPYRIKTIRNKGYLFAPHAWDE
TTG

Sequences:

>Translated_243_residues
MNRIVFVEDDAEVGSLIAAYLAKHDIDVIVEPRGDRAEDLILITQPDLVLLDIMLPGKDGMTICRDLRHRWQGPIVLLTS
LDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQSEQTQQAKSLQESALTPHKALRFGALTIDPLNRAVQLNGDFI
SLSTADFELLWELATHAGQIMDRDALLKTLRGVNYDGLDRSVDVAISRLRKKLLDSAAEPYRIKTIRNKGYLFAPHAWDE
TTG
>Mature_243_residues
MNRIVFVEDDAEVGSLIAAYLAKHDIDVIVEPRGDRAEDLILITQPDLVLLDIMLPGKDGMTICRDLRHRWQGPIVLLTS
LDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQSEQTQQAKSLQESALTPHKALRFGALTIDPLNRAVQLNGDFI
SLSTADFELLWELATHAGQIMDRDALLKTLRGVNYDGLDRSVDVAISRLRKKLLDSAAEPYRIKTIRNKGYLFAPHAWDE
TTG

Specific function: Member of the two-component regulatory system rstB/rstA [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693140, Length=239, Percent_Identity=84.9372384937239, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1789809, Length=234, Percent_Identity=32.9059829059829, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI2367329, Length=233, Percent_Identity=35.6223175965665, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788394, Length=229, Percent_Identity=33.6244541484716, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI87082012, Length=233, Percent_Identity=33.4763948497854, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1786784, Length=232, Percent_Identity=31.8965517241379, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1786599, Length=235, Percent_Identity=29.3617021276596, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1790863, Length=232, Percent_Identity=28.8793103448276, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787229, Length=214, Percent_Identity=31.3084112149533, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786911, Length=233, Percent_Identity=27.4678111587983, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789402, Length=230, Percent_Identity=29.1304347826087, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790860, Length=229, Percent_Identity=27.5109170305677, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790552, Length=237, Percent_Identity=27.0042194092827, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787375, Length=246, Percent_Identity=25.2032520325203, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 27215; Mature: 27215

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIVFVEDDAEVGSLIAAYLAKHDIDVIVEPRGDRAEDLILITQPDLVLLDIMLPGKDG
CCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCEEEEEEEECCCCC
MTICRDLRHRWQGPIVLLTSLDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQSE
HHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHH
QTQQAKSLQESALTPHKALRFGALTIDPLNRAVQLNGDFISLSTADFELLWELATHAGQI
HHHHHHHHHHHHCCHHHHHHCCCEEECCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHH
MDRDALLKTLRGVNYDGLDRSVDVAISRLRKKLLDSAAEPYRIKTIRNKGYLFAPHAWDE
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCC
TTG
CCC
>Mature Secondary Structure
MNRIVFVEDDAEVGSLIAAYLAKHDIDVIVEPRGDRAEDLILITQPDLVLLDIMLPGKDG
CCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCEEEEEEEECCCCC
MTICRDLRHRWQGPIVLLTSLDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQSE
HHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHH
QTQQAKSLQESALTPHKALRFGALTIDPLNRAVQLNGDFISLSTADFELLWELATHAGQI
HHHHHHHHHHHHCCHHHHHHCCCEEECCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHH
MDRDALLKTLRGVNYDGLDRSVDVAISRLRKKLLDSAAEPYRIKTIRNKGYLFAPHAWDE
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCC
TTG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1495392 [H]