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The map label for this gene is xthA1 [H]

Identifier: 28572897

GI number: 28572897

Start: 853087

End: 853896

Strand: Direct

Name: xthA1 [H]

Synonym: TW756

Alternate gene names: 28572897

Gene position: 853087-853896 (Clockwise)

Preceding gene: 28572895

Following gene: 28572904

Centisome position: 92.13

GC content: 47.16

Gene sequence:

>810_bases
GTGAGAATCGCAACTTGGAATGTAAACTCAATAAAAACGCGATTGAGTCAAGTCTTGCACTGGTTGACAGAAAACGAAAT
TGACATTCTCGCCCTTCAGGAAATAAAATGCAGGACCAATGCTTTTCCGGCAGAGGTTTTTATGGAGCACGGGTATTTTT
GTGCTGCCCACGGCCTTGGTGGCCGAGAGGGCGTGGCAATAGTAAGTAAATCAGAGCCACAAGACATAACAACAGACGTG
GAAGGGATTCCCGGATATGATGACCGGAATACAGGTAAACAGGATAAATCAGATGTACAAGCACGCATCATTGCAGCAAC
TGTTGGGGGTGTAAGGCTGTACAGTGTTTACGTTCCAAACGGTAGGGATGTACACAGCCCACATTACCTATATAAGCTCG
ACTGGCTTGAAGGGTTCGCGACTCATATTCGAGGGGTGATAAACACCGAGCCGAATCTGGTGATTGCCGGGGATTTTAAC
ATCGTACTATTTGATAGTGATAGCAACGACCCTCGCGTACAGACAGACACTGATGTATTTCGCACACCACATGAGCGGCA
GGCATTCTACAACCTGCTTGATTTGGGCCTTAGGGACCTTGTAAGGCCAACAGAACCAGAGGGCTTCACTTTCTGGGACT
ATCGAGCAGGGCGTTTTCAGAAAAATGAAGGCCTAAGAATAGACCATATACTGGGAACCGACCCAATTGCGAACAGCGTA
AGGTTTGCAAAAATACATCACGAGCAACGAAAAGGAGATGAGGGTAAAACTCCAAGCGATCATGTGCCCGTTATGATCGA
GCTGCGTTAG

Upstream 100 bases:

>100_bases
ACCTTCCAAACGGACCCATGAAGGGCACGCGGATCTACAGTCAACGCATAAATCCGGCGTAAATCTAAAATCCTTTGCAC
TTATCTGCTTTACTGGGATT

Downstream 100 bases:

>100_bases
TTTTATCTGCTTCAAGGCAGGCCTTGGGAGGATTCTCCCGCTTCTTAATGACAAGCCTTCCGGAAAAGAGAGGCGTTATG
ACAAAATGTCGCAGACCGAG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MRIATWNVNSIKTRLSQVLHWLTENEIDILALQEIKCRTNAFPAEVFMEHGYFCAAHGLGGREGVAIVSKSEPQDITTDV
EGIPGYDDRNTGKQDKSDVQARIIAATVGGVRLYSVYVPNGRDVHSPHYLYKLDWLEGFATHIRGVINTEPNLVIAGDFN
IVLFDSDSNDPRVQTDTDVFRTPHERQAFYNLLDLGLRDLVRPTEPEGFTFWDYRAGRFQKNEGLRIDHILGTDPIANSV
RFAKIHHEQRKGDEGKTPSDHVPVMIELR

Sequences:

>Translated_269_residues
MRIATWNVNSIKTRLSQVLHWLTENEIDILALQEIKCRTNAFPAEVFMEHGYFCAAHGLGGREGVAIVSKSEPQDITTDV
EGIPGYDDRNTGKQDKSDVQARIIAATVGGVRLYSVYVPNGRDVHSPHYLYKLDWLEGFATHIRGVINTEPNLVIAGDFN
IVLFDSDSNDPRVQTDTDVFRTPHERQAFYNLLDLGLRDLVRPTEPEGFTFWDYRAGRFQKNEGLRIDHILGTDPIANSV
RFAKIHHEQRKGDEGKTPSDHVPVMIELR
>Mature_269_residues
MRIATWNVNSIKTRLSQVLHWLTENEIDILALQEIKCRTNAFPAEVFMEHGYFCAAHGLGGREGVAIVSKSEPQDITTDV
EGIPGYDDRNTGKQDKSDVQARIIAATVGGVRLYSVYVPNGRDVHSPHYLYKLDWLEGFATHIRGVINTEPNLVIAGDFN
IVLFDSDSNDPRVQTDTDVFRTPHERQAFYNLLDLGLRDLVRPTEPEGFTFWDYRAGRFQKNEGLRIDHILGTDPIANSV
RFAKIHHEQRKGDEGKTPSDHVPVMIELR

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=278, Percent_Identity=29.4964028776978, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI18375503, Length=278, Percent_Identity=29.4964028776978, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI18375501, Length=278, Percent_Identity=29.4964028776978, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1788046, Length=282, Percent_Identity=28.7234042553192, Blast_Score=108, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71989536, Length=281, Percent_Identity=24.5551601423488, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI221330655, Length=275, Percent_Identity=30.9090909090909, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17136678, Length=275, Percent_Identity=30.9090909090909, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30482; Mature: 30482

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATWNVNSIKTRLSQVLHWLTENEIDILALQEIKCRTNAFPAEVFMEHGYFCAAHGLG
CEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEEEEHHCCCCCCHHHHHHCCCEEEECCCC
GREGVAIVSKSEPQDITTDVEGIPGYDDRNTGKQDKSDVQARIIAATVGGVRLYSVYVPN
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEEEEECCEEEEEEECCC
GRDVHSPHYLYKLDWLEGFATHIRGVINTEPNLVIAGDFNIVLFDSDSNDPRVQTDTDVF
CCCCCCCEEEEEEHHHHHHHHHHCEEECCCCCEEEEECEEEEEEECCCCCCCEECCCHHH
RTPHERQAFYNLLDLGLRDLVRPTEPEGFTFWDYRAGRFQKNEGLRIDHILGTDPIANSV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEECCCCCEEEEEECCCCCCCCE
RFAKIHHEQRKGDEGKTPSDHVPVMIELR
EEEEHHHHHHCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MRIATWNVNSIKTRLSQVLHWLTENEIDILALQEIKCRTNAFPAEVFMEHGYFCAAHGLG
CEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEEEEHHCCCCCCHHHHHHCCCEEEECCCC
GREGVAIVSKSEPQDITTDVEGIPGYDDRNTGKQDKSDVQARIIAATVGGVRLYSVYVPN
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEEEEECCEEEEEEECCC
GRDVHSPHYLYKLDWLEGFATHIRGVINTEPNLVIAGDFNIVLFDSDSNDPRVQTDTDVF
CCCCCCCEEEEEEHHHHHHHHHHCEEECCCCCEEEEECEEEEEEECCCCCCCEECCCHHH
RTPHERQAFYNLLDLGLRDLVRPTEPEGFTFWDYRAGRFQKNEGLRIDHILGTDPIANSV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEECCCCCEEEEEECCCCCCCCE
RFAKIHHEQRKGDEGKTPSDHVPVMIELR
EEEEHHHHHHCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]