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The map label for this gene is trpS

Identifier: 28572846

GI number: 28572846

Start: 799092

End: 800099

Strand: Direct

Name: trpS

Synonym: TW705

Alternate gene names: 28572846

Gene position: 799092-800099 (Clockwise)

Preceding gene: 28572845

Following gene: 28572847

Centisome position: 86.3

GC content: 45.34

Gene sequence:

>1008_bases
ATGACTTCTAGCGCAATACGGATTAACAAGAAACCGGTTGTACTGAGTGGGATACAGCCATCCTCTGGGATGTTGCACCT
TGGAAACTATCTCGGGGCGCTGAAGAGCTTCGGGCGTATGCAAGATGATTACACAACCTACTTTATGCTTGCAAATCTAC
ACTCAATGACATTCCCACAAAACCCAGAGGTCTTGCGGGAGAATACAATTAGAATTGCGGCTCAGTGTATTGCAGCCGGT
ATTGACCCAGCCAAGAGCATTGTGTTCTTACAATCCGATGTGTATCAGCACAATCAACTCGCATGGGTTCTGGGCAATGT
GTGCACATTCGGCGAGGCGGCTCGGATGACACAGTTCAAAGATAAAAGCGGAAAGCAAGGCAATATCTCTACCGGCCTTT
TTACCTACCCAATCCTGATGGCATCTGACATACTGCTGTATGACTCAGCGTTTGTCCCTGTTGGAGCTGACCAGAAGCAG
CATCTGGAGCTTACACGCACCCTGGCAAGACGGTTCAATGCACAATATGGTCAGACCTTCCTTGTGCCACAGCCATTTGA
GTGCTCGATTAGAATATACGATCTGCAAGATCCTGCTGTAAAAATGTCAAAATCCTCAGCAACCGAAAGTGGAACAATTT
TTCTTTTAGATAGCCCGGATAAAATCGTGAAAAAGATAATGCGCTCTGTAACTGACAGTGAGGATGTTATCGGATACGAC
AGGGAAACCAAGCCGGGTGTTAGCAATCTTGTAGTGATGTACTCTTGCTTGACTGATTGTACAATTGAGCAAACGGTTAA
CATATACTCTGGGAAAAAATATAGCGTCCTGAAAAAGGATCTATCGGATATTCTGGTCGAGGTGTGCACGACTATCGCAA
CCAGAACAAATGAACTTCTCGATGATAAAAACTACATAAGGAAGATTCTCGTAACGGCTTCCGAACAGGCAAGAGGAGTT
GCGCAAAAAACAATAGATCGTGTATATGAGAAGCTCGGCGTGTACTGA

Upstream 100 bases:

>100_bases
CAGTTCGCCACGCAGAGACTTAAAGCTCTTTGGGTGGCTATTGACAGGCAGAAAAGTTATGCACAAAGGTGGACTGATCA
CGCACCCGTTGTTGTGAAAT

Downstream 100 bases:

>100_bases
CCTCTTTGTGTTGGCGATGCATAGAGCCATCACTATTGCTCAGCATGGCCCCGTATTTGATAGGAATCCACAGGTTGGTT
GTGTGATTTTGGATAGAGAT

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTSSAIRINKKPVVLSGIQPSSGMLHLGNYLGALKSFGRMQDDYTTYFMLANLHSMTFPQNPEVLRENTIRIAAQCIAAG
IDPAKSIVFLQSDVYQHNQLAWVLGNVCTFGEAARMTQFKDKSGKQGNISTGLFTYPILMASDILLYDSAFVPVGADQKQ
HLELTRTLARRFNAQYGQTFLVPQPFECSIRIYDLQDPAVKMSKSSATESGTIFLLDSPDKIVKKIMRSVTDSEDVIGYD
RETKPGVSNLVVMYSCLTDCTIEQTVNIYSGKKYSVLKKDLSDILVEVCTTIATRTNELLDDKNYIRKILVTASEQARGV
AQKTIDRVYEKLGVY

Sequences:

>Translated_335_residues
MTSSAIRINKKPVVLSGIQPSSGMLHLGNYLGALKSFGRMQDDYTTYFMLANLHSMTFPQNPEVLRENTIRIAAQCIAAG
IDPAKSIVFLQSDVYQHNQLAWVLGNVCTFGEAARMTQFKDKSGKQGNISTGLFTYPILMASDILLYDSAFVPVGADQKQ
HLELTRTLARRFNAQYGQTFLVPQPFECSIRIYDLQDPAVKMSKSSATESGTIFLLDSPDKIVKKIMRSVTDSEDVIGYD
RETKPGVSNLVVMYSCLTDCTIEQTVNIYSGKKYSVLKKDLSDILVEVCTTIATRTNELLDDKNYIRKILVTASEQARGV
AQKTIDRVYEKLGVY
>Mature_334_residues
TSSAIRINKKPVVLSGIQPSSGMLHLGNYLGALKSFGRMQDDYTTYFMLANLHSMTFPQNPEVLRENTIRIAAQCIAAGI
DPAKSIVFLQSDVYQHNQLAWVLGNVCTFGEAARMTQFKDKSGKQGNISTGLFTYPILMASDILLYDSAFVPVGADQKQH
LELTRTLARRFNAQYGQTFLVPQPFECSIRIYDLQDPAVKMSKSSATESGTIFLLDSPDKIVKKIMRSVTDSEDVIGYDR
ETKPGVSNLVVMYSCLTDCTIEQTVNIYSGKKYSVLKKDLSDILVEVCTTIATRTNELLDDKNYIRKILVTASEQARGVA
QKTIDRVYEKLGVY

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=334, Percent_Identity=39.5209580838323, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI41352700, Length=185, Percent_Identity=47.5675675675676, Blast_Score=178, Evalue=5e-45,
Organism=Escherichia coli, GI1789786, Length=328, Percent_Identity=44.5121951219512, Blast_Score=284, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI71982800, Length=332, Percent_Identity=34.9397590361446, Blast_Score=190, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI71982793, Length=332, Percent_Identity=34.9397590361446, Blast_Score=188, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6320474, Length=357, Percent_Identity=35.8543417366947, Blast_Score=203, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24666151, Length=332, Percent_Identity=40.0602409638554, Blast_Score=240, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_TROW8 (Q83HC3)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789626.1
- ProteinModelPortal:   Q83HC3
- SMR:   Q83HC3
- STRING:   Q83HC3
- GeneID:   1064905
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW705
- eggNOG:   COG0180
- HOGENOM:   HBG293263
- OMA:   NKPGVSN
- PhylomeDB:   Q83HC3
- ProtClustDB:   PRK00927
- BioCyc:   TWHI218496:TW0671-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 37339; Mature: 37208

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 204-204

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSAIRINKKPVVLSGIQPSSGMLHLGNYLGALKSFGRMQDDYTTYFMLANLHSMTFPQ
CCCCCEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
NPEVLRENTIRIAAQCIAAGIDPAKSIVFLQSDVYQHNQLAWVLGNVCTFGEAARMTQFK
CCHHHHHHHHHHHHHHHHHCCCHHHHEEEEEHHHHHHCHHHHHHHHHHHCCHHHHHHHHH
DKSGKQGNISTGLFTYPILMASDILLYDSAFVPVGADQKQHLELTRTLARRFNAQYGQTF
CCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHCHHCCCEE
LVPQPFECSIRIYDLQDPAVKMSKSSATESGTIFLLDSPDKIVKKIMRSVTDSEDVIGYD
ECCCCCCCEEEEEECCCCHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHCCCC
RETKPGVSNLVVMYSCLTDCTIEQTVNIYSGKKYSVLKKDLSDILVEVCTTIATRTNELL
CCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DDKNYIRKILVTASEQARGVAQKTIDRVYEKLGVY
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSSAIRINKKPVVLSGIQPSSGMLHLGNYLGALKSFGRMQDDYTTYFMLANLHSMTFPQ
CCCCEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
NPEVLRENTIRIAAQCIAAGIDPAKSIVFLQSDVYQHNQLAWVLGNVCTFGEAARMTQFK
CCHHHHHHHHHHHHHHHHHCCCHHHHEEEEEHHHHHHCHHHHHHHHHHHCCHHHHHHHHH
DKSGKQGNISTGLFTYPILMASDILLYDSAFVPVGADQKQHLELTRTLARRFNAQYGQTF
CCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHCHHCCCEE
LVPQPFECSIRIYDLQDPAVKMSKSSATESGTIFLLDSPDKIVKKIMRSVTDSEDVIGYD
ECCCCCCCEEEEEECCCCHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHCCCC
RETKPGVSNLVVMYSCLTDCTIEQTVNIYSGKKYSVLKKDLSDILVEVCTTIATRTNELL
CCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DDKNYIRKILVTASEQARGVAQKTIDRVYEKLGVY
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174