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The map label for this gene is exoA [H]

Identifier: 28572845

GI number: 28572845

Start: 798257

End: 799102

Strand: Direct

Name: exoA [H]

Synonym: TW704

Alternate gene names: 28572845

Gene position: 798257-799102 (Clockwise)

Preceding gene: 28572844

Following gene: 28572846

Centisome position: 86.21

GC content: 47.87

Gene sequence:

>846_bases
ATGCTAGAAGTCGCCTCAGTAAATGTAAATGGGATAAGGGCGGCGCACCGTAAGGGAATGGACTTGTGGCTCAAGGAGAG
AAATCCCGACATCCTAACAATCCAGGAAGTAAGGGCGCAGGAATCGGATCTACGTGGCATCCTGCCCAATTGGAATATAT
CCTTTGATCCATGTTCAATCCCCGGTAGGGCAGGCGTTGCGATAGCGAGCAAATTTCCCCCAATAGCTGTGAGGACAAAG
CTCCGGGCAAATAAGATCCTGAACCCGGAAACTGCCAGCGAAGAGCCGCTCAATACGAGTGGTAGGTGGATTGAAGCTGA
TTATGATATCGCCGGAAAACTTGTTACCGTGGTGAGCTGTTATGTTTATGCTGGCGAGGCGAGAACAATAAAACAGGAGG
AAAAAATGGCTTTTCTGCAAGCTGTGGAAAAGCGGATGAAATCCCTTGTAAAAGAGGAGTTCGCTCTTGTAACCGGGGAT
TTCAATGTTGGACATACAACCCTGGATATAAAGAATTGGCGCGGAAATCTTGATAAGTCTGGGTTCTTACCGGAAGAAAG
AGCGTATCTGGATGCTCTGGTTGGTAACGAGTCTGATAAAGAATACAATGCTGGCGCAGGGCAAGGGTGGATTGATGTTG
TCAGAAAGATAAAAGGGGGGATCGATGGACCCTATACATGGTGGTCCATGCGTGGCAACGCTTTTGATAATGACACTGGA
TGGCGCATAGACTACCAGTTCGCCACGCAGAGACTTAAAGCTCTTTGGGTGGCTATTGACAGGCAGAAAAGTTATGCACA
AAGGTGGACTGATCACGCACCCGTTGTTGTGAAATATGACTTCTAG

Upstream 100 bases:

>100_bases
GTCACGGGGGATAAATTTGTGGGTTTCTTGCTTTAGTTCTGAGTAAAGCTGCAGATTGGTAAGAATCTCAAAGAAATGGT
GTTTTGTGTAAAATCGAGAC

Downstream 100 bases:

>100_bases
CGCAATACGGATTAACAAGAAACCGGTTGTACTGAGTGGGATACAGCCATCCTCTGGGATGTTGCACCTTGGAAACTATC
TCGGGGCGCTGAAGAGCTTC

Product: exodeoxyribonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MLEVASVNVNGIRAAHRKGMDLWLKERNPDILTIQEVRAQESDLRGILPNWNISFDPCSIPGRAGVAIASKFPPIAVRTK
LRANKILNPETASEEPLNTSGRWIEADYDIAGKLVTVVSCYVYAGEARTIKQEEKMAFLQAVEKRMKSLVKEEFALVTGD
FNVGHTTLDIKNWRGNLDKSGFLPEERAYLDALVGNESDKEYNAGAGQGWIDVVRKIKGGIDGPYTWWSMRGNAFDNDTG
WRIDYQFATQRLKALWVAIDRQKSYAQRWTDHAPVVVKYDF

Sequences:

>Translated_281_residues
MLEVASVNVNGIRAAHRKGMDLWLKERNPDILTIQEVRAQESDLRGILPNWNISFDPCSIPGRAGVAIASKFPPIAVRTK
LRANKILNPETASEEPLNTSGRWIEADYDIAGKLVTVVSCYVYAGEARTIKQEEKMAFLQAVEKRMKSLVKEEFALVTGD
FNVGHTTLDIKNWRGNLDKSGFLPEERAYLDALVGNESDKEYNAGAGQGWIDVVRKIKGGIDGPYTWWSMRGNAFDNDTG
WRIDYQFATQRLKALWVAIDRQKSYAQRWTDHAPVVVKYDF
>Mature_281_residues
MLEVASVNVNGIRAAHRKGMDLWLKERNPDILTIQEVRAQESDLRGILPNWNISFDPCSIPGRAGVAIASKFPPIAVRTK
LRANKILNPETASEEPLNTSGRWIEADYDIAGKLVTVVSCYVYAGEARTIKQEEKMAFLQAVEKRMKSLVKEEFALVTGD
FNVGHTTLDIKNWRGNLDKSGFLPEERAYLDALVGNESDKEYNAGAGQGWIDVVRKIKGGIDGPYTWWSMRGNAFDNDTG
WRIDYQFATQRLKALWVAIDRQKSYAQRWTDHAPVVVKYDF

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=283, Percent_Identity=27.9151943462898, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI18375503, Length=283, Percent_Identity=27.9151943462898, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI18375501, Length=283, Percent_Identity=27.9151943462898, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1788046, Length=293, Percent_Identity=27.6450511945392, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71989536, Length=279, Percent_Identity=25.4480286738351, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI221330655, Length=291, Percent_Identity=26.8041237113402, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17136678, Length=291, Percent_Identity=26.8041237113402, Blast_Score=91, Evalue=7e-19,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31739; Mature: 31739

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEVASVNVNGIRAAHRKGMDLWLKERNPDILTIQEVRAQESDLRGILPNWNISFDPCSI
CEEEEECCCCHHHHHHHCCCEEEEECCCCCEEEHHHHHCCHHHCCCCCCCCCCCCCCCCC
PGRAGVAIASKFPPIAVRTKLRANKILNPETASEEPLNTSGRWIEADYDIAGKLVTVVSC
CCCCCEEEECCCCCEEEEHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHH
YVYAGEARTIKQEEKMAFLQAVEKRMKSLVKEEFALVTGDFNVGHTTLDIKNWRGNLDKS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEEECCCCCCCCC
GFLPEERAYLDALVGNESDKEYNAGAGQGWIDVVRKIKGGIDGPYTWWSMRGNAFDNDTG
CCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCC
WRIDYQFATQRLKALWVAIDRQKSYAQRWTDHAPVVVKYDF
CEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure
MLEVASVNVNGIRAAHRKGMDLWLKERNPDILTIQEVRAQESDLRGILPNWNISFDPCSI
CEEEEECCCCHHHHHHHCCCEEEEECCCCCEEEHHHHHCCHHHCCCCCCCCCCCCCCCCC
PGRAGVAIASKFPPIAVRTKLRANKILNPETASEEPLNTSGRWIEADYDIAGKLVTVVSC
CCCCCEEEECCCCCEEEEHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHH
YVYAGEARTIKQEEKMAFLQAVEKRMKSLVKEEFALVTGDFNVGHTTLDIKNWRGNLDKS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEEECCCCCCCCC
GFLPEERAYLDALVGNESDKEYNAGAGQGWIDVVRKIKGGIDGPYTWWSMRGNAFDNDTG
CCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCC
WRIDYQFATQRLKALWVAIDRQKSYAQRWTDHAPVVVKYDF
CEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]