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The map label for this gene is ilvD

Identifier: 28572716

GI number: 28572716

Start: 606392

End: 608101

Strand: Reverse

Name: ilvD

Synonym: TW568

Alternate gene names: 28572716

Gene position: 608101-606392 (Counterclockwise)

Preceding gene: 28572717

Following gene: 28572715

Centisome position: 65.67

GC content: 48.83

Gene sequence:

>1710_bases
GTGTTTATGTCGAGCGATGCAAATGCCTGTGAAATAGATATAAAGCCGAGAAGCCGAGTCGTAACCCATGGGATCGAGGC
GACAACATCGCGTGGTATGCTGCGCGCCGTTGGAATGGGTGATGCTGATTGGGAAAAACCACAGATTGGCATTGCATCCA
GTTGGAATAACATAACTCCGTGCAATTTGTCACTTGACAGACTTGCGCAGGGTGCAAGGGAAGGTGTTCACGCAGCCGGT
GGGTATCCGCTGCAGTTTTGCACTATATCGGTTTCCGACGGAATCTCTATGGGTCATGAGGGAATGCATTTTTCTCTTGT
TTCTCGCGAAGTTATTACCGACAGCGTGGAAACCGTTCTGATGGCAGAGGCACTGGATGGTGTTGTTCTTCTTGCTGGCT
GTGATAAATCCCTCCCGGGTATGCTTATGGCAGCTGCAAGGCTTGATGTGTCTGCAGTATTTCTATATGCCGGCAGTATT
GCCCCCGGTTATGTCACACTCAAGTGTGGCGAGTCAAAAGAAGTTACAATAATTGATTCATTCGAAGCCGTTGGTGCCTA
TAAGGCAGGGCTTATTGATCAGGATGATCTTGGCCGGATTGAAAGGGCCATATGCCCCGGGGAGGGCGCATGTGGCGGCA
TGTATACGGCAAACACAATGGCATCTGTTGCAGAAGCGCTTGGTATGAGTCTTTTGGGTTCGGCAAGTCCACCCTCGGCA
GATCGTCGTCGTGATGTTTATGCACATAAGTCGGGCGAGGCGGTTGTTGAGCTCCTGAAGCGGGGTATAACCGCAAGAGA
TATTTTGACAAAAGAGGCATTTGAAAATGCGATAGCAGTTGTTATGGCCCTTGGCGGATCTACAAACGCCGTTTTGCATT
TACTTGCGATAGCTCATGAGGCCCATGTGCCACTGACGATAGACGATTTTAACAAAATCGGGAATAGGGTTCCTCATATT
GCAGACCTCAAGCCTTTTGGGCGCTATGTAATGAATGATGTAGATCGCGTGGGAGGTATTCCAGTTGTTATAAATGCATT
GATGCGGGAAGGTTTTATACACGGTGATGTAATTACCGTTTCAGGCAGAACAATGGCCGAGGAGATATCTGATATCAATC
CGGGCCTTCCCGACGGAAAGGTTATACACTCTTTTTCCTCACCTCTGCACCCAACAGGCGGTATAAAGGTATTAAAAGGT
ACGCTTGCACCGGATGGGGCCGTTGCAAAAACAGCTGGTTTCGATACTGTGGTTTTTCAAGGCCCCGCAATGGTGTTTGA
TCGCGAGCGCGCGGCTATGGATGCTCTCAGTGCTGGGAATATAAAAAAGGGGAGCGTTATTGTTATTCGCTATGAAGGCC
CAAAAGGGGGTCCGGGTATGCGAGAGATGCTGGCTATTACCGCGGCAATAAAGGGAAGTGGCCTTGGTAAAGATGTTTTA
TTGTTGACAGACGGCAGGTTCTCGGGCGGTACAACCGGTCTGTGTATAGGGCATATTGCGCCAGAGGCGGTTGATCTTGG
GCCGATTGCGTTTGTGCAAGATGGCGATATAATCCGCGTTGATATTGAGAAATCGAGTATTGATGTTCTGGTTGATGAAA
AGCAATTGCGCGCCAGGCACTTGACTCCACCCCCACCCAGGTATACAAGCGGTGTTCTTTCAAAATATTCAAAACTCGTC
AAATCTGCTTCTTTGGGTGCAATAACTTAG

Upstream 100 bases:

>100_bases
CTCAATATCCTTATTTGTGTATAAATGCCAGTATATTTTTTTACTATCACAGGTAATACCTTTTTAATACCCCTGTTGCG
TAGGGATTGCTGGGTACAAT

Downstream 100 bases:

>100_bases
GCAAAAGGCATTTTTCATTTTTAGTATTGTGAAGGGTTTGGATCTTGGTTAGGATATCGGGTGCACAAGCGGTTATAAAA
TCCCTTGAGGAACTTGGTAT

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MFMSSDANACEIDIKPRSRVVTHGIEATTSRGMLRAVGMGDADWEKPQIGIASSWNNITPCNLSLDRLAQGAREGVHAAG
GYPLQFCTISVSDGISMGHEGMHFSLVSREVITDSVETVLMAEALDGVVLLAGCDKSLPGMLMAAARLDVSAVFLYAGSI
APGYVTLKCGESKEVTIIDSFEAVGAYKAGLIDQDDLGRIERAICPGEGACGGMYTANTMASVAEALGMSLLGSASPPSA
DRRRDVYAHKSGEAVVELLKRGITARDILTKEAFENAIAVVMALGGSTNAVLHLLAIAHEAHVPLTIDDFNKIGNRVPHI
ADLKPFGRYVMNDVDRVGGIPVVINALMREGFIHGDVITVSGRTMAEEISDINPGLPDGKVIHSFSSPLHPTGGIKVLKG
TLAPDGAVAKTAGFDTVVFQGPAMVFDRERAAMDALSAGNIKKGSVIVIRYEGPKGGPGMREMLAITAAIKGSGLGKDVL
LLTDGRFSGGTTGLCIGHIAPEAVDLGPIAFVQDGDIIRVDIEKSSIDVLVDEKQLRARHLTPPPPRYTSGVLSKYSKLV
KSASLGAIT

Sequences:

>Translated_569_residues
MFMSSDANACEIDIKPRSRVVTHGIEATTSRGMLRAVGMGDADWEKPQIGIASSWNNITPCNLSLDRLAQGAREGVHAAG
GYPLQFCTISVSDGISMGHEGMHFSLVSREVITDSVETVLMAEALDGVVLLAGCDKSLPGMLMAAARLDVSAVFLYAGSI
APGYVTLKCGESKEVTIIDSFEAVGAYKAGLIDQDDLGRIERAICPGEGACGGMYTANTMASVAEALGMSLLGSASPPSA
DRRRDVYAHKSGEAVVELLKRGITARDILTKEAFENAIAVVMALGGSTNAVLHLLAIAHEAHVPLTIDDFNKIGNRVPHI
ADLKPFGRYVMNDVDRVGGIPVVINALMREGFIHGDVITVSGRTMAEEISDINPGLPDGKVIHSFSSPLHPTGGIKVLKG
TLAPDGAVAKTAGFDTVVFQGPAMVFDRERAAMDALSAGNIKKGSVIVIRYEGPKGGPGMREMLAITAAIKGSGLGKDVL
LLTDGRFSGGTTGLCIGHIAPEAVDLGPIAFVQDGDIIRVDIEKSSIDVLVDEKQLRARHLTPPPPRYTSGVLSKYSKLV
KSASLGAIT
>Mature_569_residues
MFMSSDANACEIDIKPRSRVVTHGIEATTSRGMLRAVGMGDADWEKPQIGIASSWNNITPCNLSLDRLAQGAREGVHAAG
GYPLQFCTISVSDGISMGHEGMHFSLVSREVITDSVETVLMAEALDGVVLLAGCDKSLPGMLMAAARLDVSAVFLYAGSI
APGYVTLKCGESKEVTIIDSFEAVGAYKAGLIDQDDLGRIERAICPGEGACGGMYTANTMASVAEALGMSLLGSASPPSA
DRRRDVYAHKSGEAVVELLKRGITARDILTKEAFENAIAVVMALGGSTNAVLHLLAIAHEAHVPLTIDDFNKIGNRVPHI
ADLKPFGRYVMNDVDRVGGIPVVINALMREGFIHGDVITVSGRTMAEEISDINPGLPDGKVIHSFSSPLHPTGGIKVLKG
TLAPDGAVAKTAGFDTVVFQGPAMVFDRERAAMDALSAGNIKKGSVIVIRYEGPKGGPGMREMLAITAAIKGSGLGKDVL
LLTDGRFSGGTTGLCIGHIAPEAVDLGPIAFVQDGDIIRVDIEKSSIDVLVDEKQLRARHLTPPPPRYTSGVLSKYSKLV
KSASLGAIT

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=610, Percent_Identity=37.7049180327869, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1788157, Length=524, Percent_Identity=32.6335877862595, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI2367371, Length=498, Percent_Identity=33.7349397590361, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1786464, Length=491, Percent_Identity=32.5865580448065, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322476, Length=559, Percent_Identity=46.690518783542, Blast_Score=501, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_TROW8 (Q83HI6)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789496.1
- ProteinModelPortal:   Q83HI6
- SMR:   Q83HI6
- STRING:   Q83HI6
- GeneID:   1064771
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW568
- eggNOG:   COG0129
- HOGENOM:   HBG671001
- OMA:   NMPGAMI
- PhylomeDB:   Q83HI6
- ProtClustDB:   PRK00911
- BioCyc:   TWHI218496:TW0541-MONOMER
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 59724; Mature: 59724

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMSSDANACEIDIKPRSRVVTHGIEATTSRGMLRAVGMGDADWEKPQIGIASSWNNITP
CCCCCCCCEEEEEECCCCHHHHCCCHHHCCCCCEEEECCCCCCCCCCCEEEECCCCCCCC
CNLSLDRLAQGAREGVHAAGGYPLQFCTISVSDGISMGHEGMHFSLVSREVITDSVETVL
CCCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHH
MAEALDGVVLLAGCDKSLPGMLMAAARLDVSAVFLYAGSIAPGYVTLKCGESKEVTIIDS
HHHHCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCEEEEEC
FEAVGAYKAGLIDQDDLGRIERAICPGEGACGGMYTANTMASVAEALGMSLLGSASPPSA
CHHHCHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
DRRRDVYAHKSGEAVVELLKRGITARDILTKEAFENAIAVVMALGGSTNAVLHLLAIAHE
CHHCCHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHH
AHVPLTIDDFNKIGNRVPHIADLKPFGRYVMNDVDRVGGIPVVINALMREGFIHGDVITV
CCCCEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEE
SGRTMAEEISDINPGLPDGKVIHSFSSPLHPTGGIKVLKGTLAPDGAVAKTAGFDTVVFQ
CCCHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCEEEEE
GPAMVFDRERAAMDALSAGNIKKGSVIVIRYEGPKGGPGMREMLAITAAIKGSGLGKDVL
CCCCEEECHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEE
LLTDGRFSGGTTGLCIGHIAPEAVDLGPIAFVQDGDIIRVDIEKSSIDVLVDEKQLRARH
EEECCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEEEECCCCEEEEECCHHHHHCC
LTPPPPRYTSGVLSKYSKLVKSASLGAIT
CCCCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFMSSDANACEIDIKPRSRVVTHGIEATTSRGMLRAVGMGDADWEKPQIGIASSWNNITP
CCCCCCCCEEEEEECCCCHHHHCCCHHHCCCCCEEEECCCCCCCCCCCEEEECCCCCCCC
CNLSLDRLAQGAREGVHAAGGYPLQFCTISVSDGISMGHEGMHFSLVSREVITDSVETVL
CCCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHH
MAEALDGVVLLAGCDKSLPGMLMAAARLDVSAVFLYAGSIAPGYVTLKCGESKEVTIIDS
HHHHCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCEEEEEC
FEAVGAYKAGLIDQDDLGRIERAICPGEGACGGMYTANTMASVAEALGMSLLGSASPPSA
CHHHCHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
DRRRDVYAHKSGEAVVELLKRGITARDILTKEAFENAIAVVMALGGSTNAVLHLLAIAHE
CHHCCHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHH
AHVPLTIDDFNKIGNRVPHIADLKPFGRYVMNDVDRVGGIPVVINALMREGFIHGDVITV
CCCCEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEE
SGRTMAEEISDINPGLPDGKVIHSFSSPLHPTGGIKVLKGTLAPDGAVAKTAGFDTVVFQ
CCCHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCEEEEE
GPAMVFDRERAAMDALSAGNIKKGSVIVIRYEGPKGGPGMREMLAITAAIKGSGLGKDVL
CCCCEEECHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEE
LLTDGRFSGGTTGLCIGHIAPEAVDLGPIAFVQDGDIIRVDIEKSSIDVLVDEKQLRARH
EEECCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEEEECCCCEEEEECCHHHHHCC
LTPPPPRYTSGVLSKYSKLVKSASLGAIT
CCCCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174