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The map label for this gene is ilvB [H]

Identifier: 28572715

GI number: 28572715

Start: 604602

End: 606347

Strand: Reverse

Name: ilvB [H]

Synonym: TW567

Alternate gene names: 28572715

Gene position: 606347-604602 (Counterclockwise)

Preceding gene: 28572716

Following gene: 28572714

Centisome position: 65.48

GC content: 46.45

Gene sequence:

>1746_bases
TTGGTTAGGATATCGGGTGCACAAGCGGTTATAAAATCCCTTGAGGAACTTGGTATTGATCTTGTTTTTGGCATTCCTGG
CGGGGCGATACTTGAGGTTTATAACTCGTTATTTAGCTCAAGCATTAGGCATATTCTTGTGAGGCATGAACAGGGCGCTG
GTCATGCTGCCGAAGGTTATGCATGTTCTTCTGGAAGGCTGGGGGTTTGCTTGGCCACCTCGGGTCCAGGCGCAACAAAT
CTTGTGACTGCCATTGCGGATGCGTATATGGATTCTGTTCCGATTCTCGCCATAACAGGTCAGGTATATTCTCATTTACT
CGGAAGTGATGCATTTCAGGAGTCGGATATTATCGGGATAACAATGCCGATTACAAAGCATGCTTTCTCTGTAAAAAGGG
CCACAGAGATCCCGGCGGCCATTTTTGCCGGTGCAAAAATTGCAACCACCGGAAGGCCAGGACCCGTTTTGATAGATATC
ACAAAAGATGCACAACAAGACGAATTCGATTTTATGTGGCCACCAAAAAGCATTGATTTATCCGGATATTCCGTTGCGCA
AAACATTCCAGATACATTGGCGCAGTTTGCAGATGGTTTTTTTGACATGCTGTCTGGCAGCATTAGGCCTGTTATTTATG
CTGGCGGAGGGACCGTGCGCTCTGGAGCCTCAGAAGAGCTTATTCGTTTTGCGGAAGGCTTTGGTATACCGGTTGTAACC
ACCCTAACCGCGCGCGGTGTTTTGCCGGATTCAAATAGGCTAAATCTCGGTATGCCGGGTATGCACGGAACTGTTGCGGC
AATTCTCGCCCTGCAGAAGTCAGATTTACTGATTGCTCTGGGTACACGTTTTGATGACCGTGTGACGGGAAGGCTTGATT
CCTTTGCACAAAATGCACGGGTTGTTCATGTGGATATTGATCCGGCTGAGATTGGCAAGAATCGACGTCCAGATCTTTTT
GTGATCGGGGACGTAAAAGAGGTGTTGGGCTATATCAACTCGCATTCGGAGAATAGAACCACGGGGGATCTTACTGCTTG
GTGGCGGTCTTTAGAGAAAATGCGTCAGATTTATCCGTTGGTTTTTCCACAAAAAAGCAGCGATGGCAATTTATCTCCCG
GTTATGTGATAAAGAGACTCGGAGAATTGTCTGGCCCGGATGCGATTTATGCAACCGGGGTTGGACAGCATCAAATGTGG
GCTTCGCAGGCTATAAGTTTTTCGAGACCCAATTCGTTTCTCACTTCCGGTGGGGCCGGAACCATGGGATACGCTGTGCC
AGCGGCTATGGGCGCTAAAGCTGCAAATCCTGAAAAAATTGTTTGGGCCATTGATGGAGATGGTTGTTTTCAAATGACCA
ATCAGGAGCTTGCAACATGTTCTGTAAACGGACTTCCAATAAAAGTTGCTGTTATAAACAACCGTTCTCTCGGGATGGTG
CGCCAGTGGCAGGGATTGTTTTATAACAAACGCTATTCTCAGACCGACCTGAATACCGGTGATGCGTATATACCTGATTT
TTGTAAACTTGCCGAATCGTATTCATGCGCAAGTTGCAGGGTTAGCTCTGTTGATGAAGTTGATAATGCAATTGAATTTG
CCCTGGCAATAAATGATAGACCGGTTGTTATAGATTTTGTCGTCTGTAAAGACTCTATGGTTTGGCCAATGGTTCCGCAA
GGGCTTGGTAATTCACATCTTAAGTATTTACAGGATGTATCACCCGTTTGGCACGATAATGAATAG

Upstream 100 bases:

>100_bases
TTCTTTCAAAATATTCAAAACTCGTCAAATCTGCTTCTTTGGGTGCAATAACTTAGGCAAAAGGCATTTTTCATTTTTAG
TATTGTGAAGGGTTTGGATC

Downstream 100 bases:

>100_bases
GGATGTAACAATCTCGGTTCTTGCAAAGGACACACCGGGGTTGCTTGCACGACTGTCATGTGTTTTTTCACGCCGTGGGA
AAAATATAATCTCATTCTCG

Product: acetolactate synthase 1 catalytic subunit

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MVRISGAQAVIKSLEELGIDLVFGIPGGAILEVYNSLFSSSIRHILVRHEQGAGHAAEGYACSSGRLGVCLATSGPGATN
LVTAIADAYMDSVPILAITGQVYSHLLGSDAFQESDIIGITMPITKHAFSVKRATEIPAAIFAGAKIATTGRPGPVLIDI
TKDAQQDEFDFMWPPKSIDLSGYSVAQNIPDTLAQFADGFFDMLSGSIRPVIYAGGGTVRSGASEELIRFAEGFGIPVVT
TLTARGVLPDSNRLNLGMPGMHGTVAAILALQKSDLLIALGTRFDDRVTGRLDSFAQNARVVHVDIDPAEIGKNRRPDLF
VIGDVKEVLGYINSHSENRTTGDLTAWWRSLEKMRQIYPLVFPQKSSDGNLSPGYVIKRLGELSGPDAIYATGVGQHQMW
ASQAISFSRPNSFLTSGGAGTMGYAVPAAMGAKAANPEKIVWAIDGDGCFQMTNQELATCSVNGLPIKVAVINNRSLGMV
RQWQGLFYNKRYSQTDLNTGDAYIPDFCKLAESYSCASCRVSSVDEVDNAIEFALAINDRPVVIDFVVCKDSMVWPMVPQ
GLGNSHLKYLQDVSPVWHDNE

Sequences:

>Translated_581_residues
MVRISGAQAVIKSLEELGIDLVFGIPGGAILEVYNSLFSSSIRHILVRHEQGAGHAAEGYACSSGRLGVCLATSGPGATN
LVTAIADAYMDSVPILAITGQVYSHLLGSDAFQESDIIGITMPITKHAFSVKRATEIPAAIFAGAKIATTGRPGPVLIDI
TKDAQQDEFDFMWPPKSIDLSGYSVAQNIPDTLAQFADGFFDMLSGSIRPVIYAGGGTVRSGASEELIRFAEGFGIPVVT
TLTARGVLPDSNRLNLGMPGMHGTVAAILALQKSDLLIALGTRFDDRVTGRLDSFAQNARVVHVDIDPAEIGKNRRPDLF
VIGDVKEVLGYINSHSENRTTGDLTAWWRSLEKMRQIYPLVFPQKSSDGNLSPGYVIKRLGELSGPDAIYATGVGQHQMW
ASQAISFSRPNSFLTSGGAGTMGYAVPAAMGAKAANPEKIVWAIDGDGCFQMTNQELATCSVNGLPIKVAVINNRSLGMV
RQWQGLFYNKRYSQTDLNTGDAYIPDFCKLAESYSCASCRVSSVDEVDNAIEFALAINDRPVVIDFVVCKDSMVWPMVPQ
GLGNSHLKYLQDVSPVWHDNE
>Mature_581_residues
MVRISGAQAVIKSLEELGIDLVFGIPGGAILEVYNSLFSSSIRHILVRHEQGAGHAAEGYACSSGRLGVCLATSGPGATN
LVTAIADAYMDSVPILAITGQVYSHLLGSDAFQESDIIGITMPITKHAFSVKRATEIPAAIFAGAKIATTGRPGPVLIDI
TKDAQQDEFDFMWPPKSIDLSGYSVAQNIPDTLAQFADGFFDMLSGSIRPVIYAGGGTVRSGASEELIRFAEGFGIPVVT
TLTARGVLPDSNRLNLGMPGMHGTVAAILALQKSDLLIALGTRFDDRVTGRLDSFAQNARVVHVDIDPAEIGKNRRPDLF
VIGDVKEVLGYINSHSENRTTGDLTAWWRSLEKMRQIYPLVFPQKSSDGNLSPGYVIKRLGELSGPDAIYATGVGQHQMW
ASQAISFSRPNSFLTSGGAGTMGYAVPAAMGAKAANPEKIVWAIDGDGCFQMTNQELATCSVNGLPIKVAVINNRSLGMV
RQWQGLFYNKRYSQTDLNTGDAYIPDFCKLAESYSCASCRVSSVDEVDNAIEFALAINDRPVVIDFVVCKDSMVWPMVPQ
GLGNSHLKYLQDVSPVWHDNE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=574, Percent_Identity=23.1707317073171, Blast_Score=146, Evalue=6e-35,
Organism=Homo sapiens, GI21361361, Length=506, Percent_Identity=26.2845849802372, Blast_Score=133, Evalue=4e-31,
Organism=Escherichia coli, GI87081685, Length=574, Percent_Identity=39.3728222996516, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1790104, Length=572, Percent_Identity=41.0839160839161, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1786717, Length=567, Percent_Identity=33.3333333333333, Blast_Score=296, Evalue=4e-81,
Organism=Escherichia coli, GI1787096, Length=558, Percent_Identity=27.4193548387097, Blast_Score=191, Evalue=2e-49,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=24.2805755395683, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=591, Percent_Identity=25.2115059221658, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=591, Percent_Identity=25.2115059221658, Blast_Score=140, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=512, Percent_Identity=25.390625, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=39.7941680960549, Blast_Score=395, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6320816, Length=488, Percent_Identity=25, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19922626, Length=583, Percent_Identity=24.3567753001715, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62405; Mature: 62405

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRISGAQAVIKSLEELGIDLVFGIPGGAILEVYNSLFSSSIRHILVRHEQGAGHAAEGY
CEEECHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCE
ACSSGRLGVCLATSGPGATNLVTAIADAYMDSVPILAITGQVYSHLLGSDAFQESDIIGI
EECCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHCCCCCCCCCEEEE
TMPITKHAFSVKRATEIPAAIFAGAKIATTGRPGPVLIDITKDAQQDEFDFMWPPKSIDL
EECHHHHHHHHHHHHCCCHHHHCCCEEEECCCCCCEEEEECCCCCCCCCCEECCCCCCCC
SGYSVAQNIPDTLAQFADGFFDMLSGSIRPVIYAGGGTVRSGASEELIRFAEGFGIPVVT
CCCHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCEEE
TLTARGVLPDSNRLNLGMPGMHGTVAAILALQKSDLLIALGTRFDDRVTGRLDSFAQNAR
EEECCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCE
VVHVDIDPAEIGKNRRPDLFVIGDVKEVLGYINSHSENRTTGDLTAWWRSLEKMRQIYPL
EEEEECCHHHHCCCCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCE
VFPQKSSDGNLSPGYVIKRLGELSGPDAIYATGVGQHQMWASQAISFSRPNSFLTSGGAG
EECCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCEECCCCC
TMGYAVPAAMGAKAANPEKIVWAIDGDGCFQMTNQELATCSVNGLPIKVAVINNRSLGMV
CCHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCCEEEEECCCEEEEEEEECCCCCCHH
RQWQGLFYNKRYSQTDLNTGDAYIPDFCKLAESYSCASCRVSSVDEVDNAIEFALAINDR
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHEEEEEEECCC
PVVIDFVVCKDSMVWPMVPQGLGNSHLKYLQDVSPVWHDNE
CEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVRISGAQAVIKSLEELGIDLVFGIPGGAILEVYNSLFSSSIRHILVRHEQGAGHAAEGY
CEEECHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCE
ACSSGRLGVCLATSGPGATNLVTAIADAYMDSVPILAITGQVYSHLLGSDAFQESDIIGI
EECCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHCCCCCCCCCEEEE
TMPITKHAFSVKRATEIPAAIFAGAKIATTGRPGPVLIDITKDAQQDEFDFMWPPKSIDL
EECHHHHHHHHHHHHCCCHHHHCCCEEEECCCCCCEEEEECCCCCCCCCCEECCCCCCCC
SGYSVAQNIPDTLAQFADGFFDMLSGSIRPVIYAGGGTVRSGASEELIRFAEGFGIPVVT
CCCHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCEEE
TLTARGVLPDSNRLNLGMPGMHGTVAAILALQKSDLLIALGTRFDDRVTGRLDSFAQNAR
EEECCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCE
VVHVDIDPAEIGKNRRPDLFVIGDVKEVLGYINSHSENRTTGDLTAWWRSLEKMRQIYPL
EEEEECCHHHHCCCCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCE
VFPQKSSDGNLSPGYVIKRLGELSGPDAIYATGVGQHQMWASQAISFSRPNSFLTSGGAG
EECCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCEECCCCC
TMGYAVPAAMGAKAANPEKIVWAIDGDGCFQMTNQELATCSVNGLPIKVAVINNRSLGMV
CCHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCCEEEEECCCEEEEEEEECCCCCCHH
RQWQGLFYNKRYSQTDLNTGDAYIPDFCKLAESYSCASCRVSSVDEVDNAIEFALAINDR
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHEEEEEEECCC
PVVIDFVVCKDSMVWPMVPQGLGNSHLKYLQDVSPVWHDNE
CEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]