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The map label for this gene is ksgA [H]

Identifier: 28572717

GI number: 28572717

Start: 616652

End: 617908

Strand: Reverse

Name: ksgA [H]

Synonym: TW569

Alternate gene names: 28572717

Gene position: 617908-616652 (Counterclockwise)

Preceding gene: 28572718

Following gene: 28572716

Centisome position: 66.73

GC content: 48.05

Gene sequence:

>1257_bases
GTGATTCACGGACATGACTATTATTCTGCGGTAAAGCGATTCGTTCCTTATAAACGTTTTGGTCAACATTTTGTTACTGA
CCCTAATATATGTAACAAAATTGTTACTCTGGCCGGCGTTGCCCCGGGTGATTGCGTTTTGGAGATTGGCCCCGGTTTTG
GTGCGCTAACCGTTGCACTGGCCAAGGCAAAGGCAACGGTGTGTGGGGTTGAAATTGACACAAGGTTGTTTAACGCTCTT
TGTAAGGTTATTCCCGCTGCATTGTCTGCTCCTCAACCGTGCACAGGTCAATCTCATACGGATCAACCGTGTGACCTGGG
CCGGTTGTGTGATCCGCATGAGTTACCACATCGATTCACCGATGAACGTACTAACAAATTACCCACTTCACAGTTACACG
GGTTACAGTTACACGGGTTACAACACCTAACAAACCAAGGGAGATGTGACACAGGATTACAGGGAGATGTGACACAAGGT
GTTAGGCACAGCACTAACCTGAACAGGGATGATCAACGGGGGGCCAATCAACACAGCAACAGACAGTACAGTGACGGGCA
GTACAGATCAGTGCAACACAGCACTGTTGAGGAGAGTATCCTGGAGGCAGAAAAGCTATCTATTGCAGCCCGCACAAATA
CACACTCTACAGATACACGGCACGATAACACGCATGAGCACATGCGGAAATGGGGTGAAGTGTATTTGATAAATGACGAT
GCCTTAAAGTTGCGAGCCTTGCCCATTACCCCAAAGATGTTGGTTGCTAATCTCCCGTACAATATCGCGGTGCCGCTTAT
AATGCACGTCTTGCAACAATTTCCCAGTATCGTATCTGCAACTATTATGCTGCAGAGTGAAGTTGCGGATCGTATATGCG
CTAAGCCACCTTCCAGATCCGCTGGTGCGGTGACTGCCAAAGCGGCGTGGTTCGGTCACTGGAAAAAGGTTATGAAGGTC
GCAAGGCATGTTTTTTACCCCATTCCCGGTGTTGATTCCGTAATTGTGCAATTTATCAGAGATGAGAAGAGCGATTGTGA
AGTTTTGCGGGAAGCGACCTTTTCTTTGATTGAGGATGCATTTTCCAAGAGGCGCAAAATGCTCAGGCAATCTTTGAGAT
ACATTCCCCAGGCTGATTTTGTCGCGGCTGGGATTGATCCCATGCTGCGCGCGGAGAATCTGTCTGTTGATAACTTTATG
AATCTTGCGAAACGTCGTGTATACTCGACGGGCCGGAACCTTACCCATTTGGCCTAG

Upstream 100 bases:

>100_bases
GTGTTGAAGCTAATACAGGAACATATACCTTCCCTGTTGTTGTAAATGGTGTGTATACCGTTCCATATACAGTGGTTGTG
ACAGAGTTACCATCACAGGT

Downstream 100 bases:

>100_bases
CTCTGGGTTTTGCTTTGGAATAAGTTTTGTAGCACCATGTTTTGTGTGTTACCCTACAGTGTTACTTTTCCGTCAATATT
CAACGGTAAGCTTGTTTTTA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MIHGHDYYSAVKRFVPYKRFGQHFVTDPNICNKIVTLAGVAPGDCVLEIGPGFGALTVALAKAKATVCGVEIDTRLFNAL
CKVIPAALSAPQPCTGQSHTDQPCDLGRLCDPHELPHRFTDERTNKLPTSQLHGLQLHGLQHLTNQGRCDTGLQGDVTQG
VRHSTNLNRDDQRGANQHSNRQYSDGQYRSVQHSTVEESILEAEKLSIAARTNTHSTDTRHDNTHEHMRKWGEVYLINDD
ALKLRALPITPKMLVANLPYNIAVPLIMHVLQQFPSIVSATIMLQSEVADRICAKPPSRSAGAVTAKAAWFGHWKKVMKV
ARHVFYPIPGVDSVIVQFIRDEKSDCEVLREATFSLIEDAFSKRRKMLRQSLRYIPQADFVAAGIDPMLRAENLSVDNFM
NLAKRRVYSTGRNLTHLA

Sequences:

>Translated_418_residues
MIHGHDYYSAVKRFVPYKRFGQHFVTDPNICNKIVTLAGVAPGDCVLEIGPGFGALTVALAKAKATVCGVEIDTRLFNAL
CKVIPAALSAPQPCTGQSHTDQPCDLGRLCDPHELPHRFTDERTNKLPTSQLHGLQLHGLQHLTNQGRCDTGLQGDVTQG
VRHSTNLNRDDQRGANQHSNRQYSDGQYRSVQHSTVEESILEAEKLSIAARTNTHSTDTRHDNTHEHMRKWGEVYLINDD
ALKLRALPITPKMLVANLPYNIAVPLIMHVLQQFPSIVSATIMLQSEVADRICAKPPSRSAGAVTAKAAWFGHWKKVMKV
ARHVFYPIPGVDSVIVQFIRDEKSDCEVLREATFSLIEDAFSKRRKMLRQSLRYIPQADFVAAGIDPMLRAENLSVDNFM
NLAKRRVYSTGRNLTHLA
>Mature_418_residues
MIHGHDYYSAVKRFVPYKRFGQHFVTDPNICNKIVTLAGVAPGDCVLEIGPGFGALTVALAKAKATVCGVEIDTRLFNAL
CKVIPAALSAPQPCTGQSHTDQPCDLGRLCDPHELPHRFTDERTNKLPTSQLHGLQLHGLQHLTNQGRCDTGLQGDVTQG
VRHSTNLNRDDQRGANQHSNRQYSDGQYRSVQHSTVEESILEAEKLSIAARTNTHSTDTRHDNTHEHMRKWGEVYLINDD
ALKLRALPITPKMLVANLPYNIAVPLIMHVLQQFPSIVSATIMLQSEVADRICAKPPSRSAGAVTAKAAWFGHWKKVMKV
ARHVFYPIPGVDSVIVQFIRDEKSDCEVLREATFSLIEDAFSKRRKMLRQSLRYIPQADFVAAGIDPMLRAENLSVDNFM
NLAKRRVYSTGRNLTHLA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786236, Length=159, Percent_Identity=33.9622641509434, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 46609; Mature: 46609

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHGHDYYSAVKRFVPYKRFGQHFVTDPNICNKIVTLAGVAPGDCVLEIGPGFGALTVAL
CCCCHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHH
AKAKATVCGVEIDTRLFNALCKVIPAALSAPQPCTGQSHTDQPCDLGRLCDPHELPHRFT
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHH
DERTNKLPTSQLHGLQLHGLQHLTNQGRCDTGLQGDVTQGVRHSTNLNRDDQRGANQHSN
HHHHCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHCCCCCCCC
RQYSDGQYRSVQHSTVEESILEAEKLSIAARTNTHSTDTRHDNTHEHMRKWGEVYLINDD
CCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHCCCEEEEECC
ALKLRALPITPKMLVANLPYNIAVPLIMHVLQQFPSIVSATIMLQSEVADRICAKPPSRS
CEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AGAVTAKAAWFGHWKKVMKVARHVFYPIPGVDSVIVQFIRDEKSDCEVLREATFSLIEDA
CCCEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FSKRRKMLRQSLRYIPQADFVAAGIDPMLRAENLSVDNFMNLAKRRVYSTGRNLTHLA
HHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIHGHDYYSAVKRFVPYKRFGQHFVTDPNICNKIVTLAGVAPGDCVLEIGPGFGALTVAL
CCCCHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHH
AKAKATVCGVEIDTRLFNALCKVIPAALSAPQPCTGQSHTDQPCDLGRLCDPHELPHRFT
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHH
DERTNKLPTSQLHGLQLHGLQHLTNQGRCDTGLQGDVTQGVRHSTNLNRDDQRGANQHSN
HHHHCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHCCCCCCCC
RQYSDGQYRSVQHSTVEESILEAEKLSIAARTNTHSTDTRHDNTHEHMRKWGEVYLINDD
CCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHCCCEEEEECC
ALKLRALPITPKMLVANLPYNIAVPLIMHVLQQFPSIVSATIMLQSEVADRICAKPPSRS
CEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AGAVTAKAAWFGHWKKVMKVARHVFYPIPGVDSVIVQFIRDEKSDCEVLREATFSLIEDA
CCCEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FSKRRKMLRQSLRYIPQADFVAAGIDPMLRAENLSVDNFMNLAKRRVYSTGRNLTHLA
HHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA