Click here to switch to the map view.

The map label for this gene is tpiA

Identifier: 28572619

GI number: 28572619

Start: 503561

End: 504442

Strand: Reverse

Name: tpiA

Synonym: TW470

Alternate gene names: 28572619

Gene position: 504442-503561 (Counterclockwise)

Preceding gene: 28572620

Following gene: 28572618

Centisome position: 54.48

GC content: 43.2

Gene sequence:

>882_bases
ATGCTTCCAAAGCGCGCGTATCCCGTGCAAGACTCGAATGTTCACTCTCAAGAGAAAAAAATAATTGCCGGCAATTGGAA
GATGAACATAAATCATAGTCAGGCTGTTTCGTATTTACAGGAGCTCAACTGGAGGCTTATTGATAATGGTCATGATTTTG
ATGCCTGTGAGATTGCAGTATTCCCGCCGTTCACTGATCTTCGTTCCGTACAAACTCTCGTTGCATCGGATGACATACAG
ATATCATACGGAGCCCAGGATGTCTCCGCATTTTCAGATGGCGCACATACAGGTCAGATTTCAGCCCAGTTTTTGAAAGA
TCTAGACTGCAAGTACGTCCTAATAGGTCATTCAGAGCAAAGATGTTTACCATGCTACCCCGGCAACAATAGTGCAATCA
ATGAGCTCAATAACAAGCACGACGGTCTTATTGCAAATAAATTATTGAGAAGCTTTGCGGCGGGGATTTGTCCGATCTTG
TGTATTGGTGATATTTCACCGGGTGATCATTTTGATGCAACTCTGTCTAGATTTCGTTCTGTTCTAAGTCACCTCAAAGC
GATATCAGATAAAAAACATAGCATCGGTTACGCCCTTGGATCCAAAACACACTTTTTAGATAGCGATCAGTTACACATGT
TGGTTGCTTATGAACCCTCATCGGCTATTAACTCGGGAAACTGTGCAAATTCAGGAGACATTGTCAGGATGGCTGCAGCA
ATAAAAGACATTGTAAATGTTCGGGTGCTCTACGGTGGGGGAGTGAATCTATTTAACGCATCTGCAGTTTTTAACGAGGA
TTTGCTGGACGGCATTCTTGTTGGCAGAGCTTCCTTGAATGCATCTGATTTCGCATCCCTGATAAAGACGTGCTGTTTGT
AA

Upstream 100 bases:

>100_bases
GGTTTTGATTATGGAGATTTTAGTCATGTTTCAACAGGCGGTGGTGCCTGCCTTGAACTGCTAGAGGGAAAAATATTGCC
AGCATTAGAGGTTTTGTAAA

Downstream 100 bases:

>100_bases
ATCTGGTGTATACTCGGGTTTGCGAACTGCATGTGTTTGTGCTTTCTCGGCTTTGTGCCTGTTTTTTTGACTGAGGATGT
TTTGATCGTTCTAAAGCTTG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MLPKRAYPVQDSNVHSQEKKIIAGNWKMNINHSQAVSYLQELNWRLIDNGHDFDACEIAVFPPFTDLRSVQTLVASDDIQ
ISYGAQDVSAFSDGAHTGQISAQFLKDLDCKYVLIGHSEQRCLPCYPGNNSAINELNNKHDGLIANKLLRSFAAGICPIL
CIGDISPGDHFDATLSRFRSVLSHLKAISDKKHSIGYALGSKTHFLDSDQLHMLVAYEPSSAINSGNCANSGDIVRMAAA
IKDIVNVRVLYGGGVNLFNASAVFNEDLLDGILVGRASLNASDFASLIKTCCL

Sequences:

>Translated_293_residues
MLPKRAYPVQDSNVHSQEKKIIAGNWKMNINHSQAVSYLQELNWRLIDNGHDFDACEIAVFPPFTDLRSVQTLVASDDIQ
ISYGAQDVSAFSDGAHTGQISAQFLKDLDCKYVLIGHSEQRCLPCYPGNNSAINELNNKHDGLIANKLLRSFAAGICPIL
CIGDISPGDHFDATLSRFRSVLSHLKAISDKKHSIGYALGSKTHFLDSDQLHMLVAYEPSSAINSGNCANSGDIVRMAAA
IKDIVNVRVLYGGGVNLFNASAVFNEDLLDGILVGRASLNASDFASLIKTCCL
>Mature_293_residues
MLPKRAYPVQDSNVHSQEKKIIAGNWKMNINHSQAVSYLQELNWRLIDNGHDFDACEIAVFPPFTDLRSVQTLVASDDIQ
ISYGAQDVSAFSDGAHTGQISAQFLKDLDCKYVLIGHSEQRCLPCYPGNNSAINELNNKHDGLIANKLLRSFAAGICPIL
CIGDISPGDHFDATLSRFRSVLSHLKAISDKKHSIGYALGSKTHFLDSDQLHMLVAYEPSSAINSGNCANSGDIVRMAAA
IKDIVNVRVLYGGGVNLFNASAVFNEDLLDGILVGRASLNASDFASLIKTCCL

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=282, Percent_Identity=26.5957446808511, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI226529917, Length=289, Percent_Identity=26.2975778546713, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1790353, Length=282, Percent_Identity=27.6595744680851, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17536593, Length=282, Percent_Identity=28.7234042553192, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320255, Length=281, Percent_Identity=27.7580071174377, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28572004, Length=290, Percent_Identity=25.8620689655172, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28572008, Length=278, Percent_Identity=25.5395683453237, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28572006, Length=278, Percent_Identity=25.5395683453237, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_TROW8 (Q83HP9)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789399.1
- ProteinModelPortal:   Q83HP9
- SMR:   Q83HP9
- STRING:   Q83HP9
- GeneID:   1064310
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW470
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   CLSK229793
- BioCyc:   TWHI218496:TW0444-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 31842; Mature: 31842

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 117-117 ACT_SITE 218-218 BINDING 25-25 BINDING 27-27

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPKRAYPVQDSNVHSQEKKIIAGNWKMNINHSQAVSYLQELNWRLIDNGHDFDACEIAV
CCCCCCCCCCCCCCCCCCCEEEECCEEEECCHHHHHHHHHHCCEEEECCCCCCCEEEEEE
FPPFTDLRSVQTLVASDDIQISYGAQDVSAFSDGAHTGQISAQFLKDLDCKYVLIGHSEQ
ECCCHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEECCCC
RCLPCYPGNNSAINELNNKHDGLIANKLLRSFAAGICPILCIGDISPGDHFDATLSRFRS
CEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHH
VLSHLKAISDKKHSIGYALGSKTHFLDSDQLHMLVAYEPSSAINSGNCANSGDIVRMAAA
HHHHHHHHCCCCHHHEEEECCCCEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHH
IKDIVNVRVLYGGGVNLFNASAVFNEDLLDGILVGRASLNASDFASLIKTCCL
HHHHHEEEEEECCCCCEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLPKRAYPVQDSNVHSQEKKIIAGNWKMNINHSQAVSYLQELNWRLIDNGHDFDACEIAV
CCCCCCCCCCCCCCCCCCCEEEECCEEEECCHHHHHHHHHHCCEEEECCCCCCCEEEEEE
FPPFTDLRSVQTLVASDDIQISYGAQDVSAFSDGAHTGQISAQFLKDLDCKYVLIGHSEQ
ECCCHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEECCCC
RCLPCYPGNNSAINELNNKHDGLIANKLLRSFAAGICPILCIGDISPGDHFDATLSRFRS
CEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHH
VLSHLKAISDKKHSIGYALGSKTHFLDSDQLHMLVAYEPSSAINSGNCANSGDIVRMAAA
HHHHHHHHCCCCHHHEEEECCCCEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHH
IKDIVNVRVLYGGGVNLFNASAVFNEDLLDGILVGRASLNASDFASLIKTCCL
HHHHHEEEEEECCCCCEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174