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The map label for this gene is pgk

Identifier: 28572620

GI number: 28572620

Start: 504444

End: 505631

Strand: Reverse

Name: pgk

Synonym: TW471

Alternate gene names: 28572620

Gene position: 505631-504444 (Counterclockwise)

Preceding gene: 28572621

Following gene: 28572619

Centisome position: 54.61

GC content: 44.61

Gene sequence:

>1188_bases
TTGAGAACTCTTGGCGGTTTCACCTTTCCTTGTGATGCTCCTGTTTTTGTCCGGGTTGATTTCAATGTGCCTATGGATGA
GGTCGGCACAATAACGGATGATCTGCGTATAAGAGCTTCCCTTCCGACTATAGAAAGCTTGCTTGGCCGTGGCGCGCCAC
TTATATTGATTTCTCATCTCGGGCGACCGGTTAAAAATGAGCAGCAAGGATTTTCCCTAAAACCCTGTGCTGAGCGACTA
TCGGAATATATCGGAGTCAATGTACCGCTTGTGGATTTTCTTGATCTAGATACAAAGCTTTATGGTGAACTTACTAGGCA
TCTGGACAAGTCTGGGATAGTTCTTTTTGAAAATATACGATTTTTTGCCGAGGAAACTTCAAAGGCACAGGCTGATCGAA
GGATTCTGGCTGAGCAACTTGCGCCTTTTGCGGGTGCGTATGTCAATGATGCCTTTGGTGCTTCCCACAGGAGGCATGCT
AGTGTGTATGAGCTTGCACAGGCTTTCTCAAATAAGGCGGCAGGCTTTCTCATTGAATCAGAGATGCAGGCTTTTTCACA
TCTTGCCAGCGAGGCCAAGCGTCCCTATACCGTTATTCTCGGCGGGGCAAAATTATCGGACAAGCTACGGCTTATAGAGA
ATATCCTGCCAACGGTTGATCGTCTGTTACTGTGTGGTGGAATGGCATTTACATTTCTAGCGGCACAGGGGTGTGAAACA
GGCAAAAGTCTCCTGGAAGAGAGTTTTATCTCGGAGGCAAATAAAATCATTGATTTTGCTAAGCAAAATAATGTTGAACT
TATACTTCCGGTAGATGTTATTGAGGCGCGAAGCCTAACTTCACCCCTTGGGGTTGTAAGTAAAGCTGAATCTATATCTT
ATATGGGTCTTGATATAGGTCCTGAAACTCAGAGTATCTTTAGAAATGTAATACAAGATTCGAAAACTGTTTTTTGGAAT
GGCCCGGCTGGTTTATTTGAAAACCCAAGCTTTTCAAACGGAACACGTGCACTGCTTGATGCATTGTCGTCTTCTAGCGC
GTTTACATTTATCGGAGGAGGCGATACAGCAGCAGCTGTCAGCCTTCTTGGTTTTGATTATGGAGATTTTAGTCATGTTT
CAACAGGCGGTGGTGCCTGCCTTGAACTGCTAGAGGGAAAAATATTGCCAGCATTAGAGGTTTTGTAA

Upstream 100 bases:

>100_bases
TATGGTCAAGCTATCTGCCTGGTATGACAATGAATGGGCATATTCTCGGCGGCTGGTTGATTTGGCCAAATATATGGCGT
CTGTTGGGCTCTGAGTTGCC

Downstream 100 bases:

>100_bases
AATGCTTCCAAAGCGCGCGTATCCCGTGCAAGACTCGAATGTTCACTCTCAAGAGAAAAAAATAATTGCCGGCAATTGGA
AGATGAACATAAATCATAGT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MRTLGGFTFPCDAPVFVRVDFNVPMDEVGTITDDLRIRASLPTIESLLGRGAPLILISHLGRPVKNEQQGFSLKPCAERL
SEYIGVNVPLVDFLDLDTKLYGELTRHLDKSGIVLFENIRFFAEETSKAQADRRILAEQLAPFAGAYVNDAFGASHRRHA
SVYELAQAFSNKAAGFLIESEMQAFSHLASEAKRPYTVILGGAKLSDKLRLIENILPTVDRLLLCGGMAFTFLAAQGCET
GKSLLEESFISEANKIIDFAKQNNVELILPVDVIEARSLTSPLGVVSKAESISYMGLDIGPETQSIFRNVIQDSKTVFWN
GPAGLFENPSFSNGTRALLDALSSSSAFTFIGGGDTAAAVSLLGFDYGDFSHVSTGGGACLELLEGKILPALEVL

Sequences:

>Translated_395_residues
MRTLGGFTFPCDAPVFVRVDFNVPMDEVGTITDDLRIRASLPTIESLLGRGAPLILISHLGRPVKNEQQGFSLKPCAERL
SEYIGVNVPLVDFLDLDTKLYGELTRHLDKSGIVLFENIRFFAEETSKAQADRRILAEQLAPFAGAYVNDAFGASHRRHA
SVYELAQAFSNKAAGFLIESEMQAFSHLASEAKRPYTVILGGAKLSDKLRLIENILPTVDRLLLCGGMAFTFLAAQGCET
GKSLLEESFISEANKIIDFAKQNNVELILPVDVIEARSLTSPLGVVSKAESISYMGLDIGPETQSIFRNVIQDSKTVFWN
GPAGLFENPSFSNGTRALLDALSSSSAFTFIGGGDTAAAVSLLGFDYGDFSHVSTGGGACLELLEGKILPALEVL
>Mature_395_residues
MRTLGGFTFPCDAPVFVRVDFNVPMDEVGTITDDLRIRASLPTIESLLGRGAPLILISHLGRPVKNEQQGFSLKPCAERL
SEYIGVNVPLVDFLDLDTKLYGELTRHLDKSGIVLFENIRFFAEETSKAQADRRILAEQLAPFAGAYVNDAFGASHRRHA
SVYELAQAFSNKAAGFLIESEMQAFSHLASEAKRPYTVILGGAKLSDKLRLIENILPTVDRLLLCGGMAFTFLAAQGCET
GKSLLEESFISEANKIIDFAKQNNVELILPVDVIEARSLTSPLGVVSKAESISYMGLDIGPETQSIFRNVIQDSKTVFWN
GPAGLFENPSFSNGTRALLDALSSSSAFTFIGGGDTAAAVSLLGFDYGDFSHVSTGGGACLELLEGKILPALEVL

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI31543397, Length=405, Percent_Identity=38.7654320987654, Blast_Score=251, Evalue=6e-67,
Organism=Homo sapiens, GI4505763, Length=405, Percent_Identity=37.7777777777778, Blast_Score=246, Evalue=4e-65,
Organism=Escherichia coli, GI1789294, Length=387, Percent_Identity=38.7596899224806, Blast_Score=243, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17508823, Length=404, Percent_Identity=40.5940594059406, Blast_Score=277, Evalue=7e-75,
Organism=Saccharomyces cerevisiae, GI6319857, Length=406, Percent_Identity=40.1477832512315, Blast_Score=257, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24581203, Length=403, Percent_Identity=40.4466501240695, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI17136394, Length=401, Percent_Identity=38.9027431421446, Blast_Score=258, Evalue=6e-69,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_TROW8 (Q83HP8)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789400.1
- ProteinModelPortal:   Q83HP8
- SMR:   Q83HP8
- STRING:   Q83HP8
- GeneID:   1064311
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW471
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- ProtClustDB:   PRK00073
- BioCyc:   TWHI218496:TW0445-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42679; Mature: 42679

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 120-120 BINDING 157-157 BINDING 208-208 BINDING 296-296 BINDING 327-327

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLGGFTFPCDAPVFVRVDFNVPMDEVGTITDDLRIRASLPTIESLLGRGAPLILISHL
CCCCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCEEEECCCHHHHHHCCCCCEEEHHHC
GRPVKNEQQGFSLKPCAERLSEYIGVNVPLVDFLDLDTKLYGELTRHLDKSGIVLFENIR
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHCCCCCEEEEEHHH
FFAEETSKAQADRRILAEQLAPFAGAYVNDAFGASHRRHASVYELAQAFSNKAAGFLIES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEHH
EMQAFSHLASEAKRPYTVILGGAKLSDKLRLIENILPTVDRLLLCGGMAFTFLAAQGCET
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
GKSLLEESFISEANKIIDFAKQNNVELILPVDVIEARSLTSPLGVVSKAESISYMGLDIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCHHHHHHHHCCCEEEECCCC
PETQSIFRNVIQDSKTVFWNGPAGLFENPSFSNGTRALLDALSSSSAFTFIGGGDTAAAV
CCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHH
SLLGFDYGDFSHVSTGGGACLELLEGKILPALEVL
HHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHCC
>Mature Secondary Structure
MRTLGGFTFPCDAPVFVRVDFNVPMDEVGTITDDLRIRASLPTIESLLGRGAPLILISHL
CCCCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCEEEECCCHHHHHHCCCCCEEEHHHC
GRPVKNEQQGFSLKPCAERLSEYIGVNVPLVDFLDLDTKLYGELTRHLDKSGIVLFENIR
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHCCCCCEEEEEHHH
FFAEETSKAQADRRILAEQLAPFAGAYVNDAFGASHRRHASVYELAQAFSNKAAGFLIES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEHH
EMQAFSHLASEAKRPYTVILGGAKLSDKLRLIENILPTVDRLLLCGGMAFTFLAAQGCET
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
GKSLLEESFISEANKIIDFAKQNNVELILPVDVIEARSLTSPLGVVSKAESISYMGLDIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCHHHHHHHHCCCEEEECCCC
PETQSIFRNVIQDSKTVFWNGPAGLFENPSFSNGTRALLDALSSSSAFTFIGGGDTAAAV
CCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHH
SLLGFDYGDFSHVSTGGGACLELLEGKILPALEVL
HHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174