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The map label for this gene is rfbM [H]

Identifier: 28572566

GI number: 28572566

Start: 451660

End: 452754

Strand: Reverse

Name: rfbM [H]

Synonym: TW414

Alternate gene names: 28572566

Gene position: 452754-451660 (Counterclockwise)

Preceding gene: 28572579

Following gene: 28572544

Centisome position: 48.9

GC content: 47.67

Gene sequence:

>1095_bases
ATGGACTGCTCAGAGCTCTACAGTGTCATCCCTGCGGGAGGAATAGGGTCCCGCTTGTGGCCACTCTCGAGAGCGGCGTC
GCCTAAGTTCCTACACAAACTGACCGGGACTGAAACCTCGTTGCTGCGCGATACATGGGATCGTCTTGTGCCCATCTCTG
GGGTAGATCGAATTATGGTAGTGATCGGCTCTGACCACTTCGACGCAGTTACATCCCAGCTACCGGAACTTAAGGGGCAT
AACGTAATTCTGGAGCCAGAACCCAAAGACTCAACAGCAGCAATATGCCTTGCCGCGGCCATTCTGAATACTAGAGAGCC
TGATGTGATTATTGGCTCATTCCCCGCCGATCATGTGATAAAAACCTCTCCCCTGTTTCAGCAATCTGTTGCACAGGCCG
TAGTAGCGGCAAATGAAGGATATGTTGTAACAATTGCAATTACCCCAACTGAGCCTGCAACACAGTTTGGATACATATTA
TGTGGCGATTCAGCTGGAATAAAGGGGGCACCGCAGGTCCTGTCGGTAAAAAGATTTGTTGAAAAACCTAGCCAGAAAGA
TGCAGACCGCTATCTATCTTCTGGAAAATATTTGTGGAATGCCGGTATGTTCATAGCTAAGGCATGTGTTTTGCTTGAAC
AGCTTGAAAAGGCAAGACCTGACATACACAGCCTGATAATAGACATTGCCAATAGCCCTGATTTGGATTCTGCGCTAAAA
AAGAATTGGCCAAAAATGCCTCGTATTGCAATAGAATACTCTGTCGCAGAGCCGTGCGCCGCAGAAGGTAGGATGGTGAC
TGTCCCGGCGAAGTTTGAATGGAACGACGCGGGAGATTTTTCATCAATTGCAAGACTAAACTCACGGGGAGGGAGCGACC
TTGTTATTCTCGGGGAAACTAGCCGCGTACTCTCGGATTCAGCCACAGGGATAGTTGTAAGTCAGACCGACAGAATAATC
TCTGTGATAGGCACAAAAGACATAGTTGTTGTAGATACGCCCGATTCTCTTTTGGTTACAACAAAAGATGATGCAAAGAA
AGTAAAACAGATAGTCGAGGCACTGCGATTGTCTGGCCGAGATGATGTATTGTAA

Upstream 100 bases:

>100_bases
CATTCGATTACAAGAAGATAGGTTAGAATTCAGTTCTGGAGCATAACCTTCTTGAAGTGGTGGAACGTACCCATCTCTGG
GGTAGAATTTTGGATGGTTT

Downstream 100 bases:

>100_bases
CCCTATTGGGTGACCAGATCGAATGGCTTCGAACAGGCAAGTATCTCTAAGATCTTTTATAAGCCACTTTGGCAAGTTTC
CTGAGGGACTCGACGCTATC

Product: sugar-1-phosphate nucleotidyltransferase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP; GTP--mannose-1-phosphate guanylyltransferase [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MDCSELYSVIPAGGIGSRLWPLSRAASPKFLHKLTGTETSLLRDTWDRLVPISGVDRIMVVIGSDHFDAVTSQLPELKGH
NVILEPEPKDSTAAICLAAAILNTREPDVIIGSFPADHVIKTSPLFQQSVAQAVVAANEGYVVTIAITPTEPATQFGYIL
CGDSAGIKGAPQVLSVKRFVEKPSQKDADRYLSSGKYLWNAGMFIAKACVLLEQLEKARPDIHSLIIDIANSPDLDSALK
KNWPKMPRIAIEYSVAEPCAAEGRMVTVPAKFEWNDAGDFSSIARLNSRGGSDLVILGETSRVLSDSATGIVVSQTDRII
SVIGTKDIVVVDTPDSLLVTTKDDAKKVKQIVEALRLSGRDDVL

Sequences:

>Translated_364_residues
MDCSELYSVIPAGGIGSRLWPLSRAASPKFLHKLTGTETSLLRDTWDRLVPISGVDRIMVVIGSDHFDAVTSQLPELKGH
NVILEPEPKDSTAAICLAAAILNTREPDVIIGSFPADHVIKTSPLFQQSVAQAVVAANEGYVVTIAITPTEPATQFGYIL
CGDSAGIKGAPQVLSVKRFVEKPSQKDADRYLSSGKYLWNAGMFIAKACVLLEQLEKARPDIHSLIIDIANSPDLDSALK
KNWPKMPRIAIEYSVAEPCAAEGRMVTVPAKFEWNDAGDFSSIARLNSRGGSDLVILGETSRVLSDSATGIVVSQTDRII
SVIGTKDIVVVDTPDSLLVTTKDDAKKVKQIVEALRLSGRDDVL
>Mature_364_residues
MDCSELYSVIPAGGIGSRLWPLSRAASPKFLHKLTGTETSLLRDTWDRLVPISGVDRIMVVIGSDHFDAVTSQLPELKGH
NVILEPEPKDSTAAICLAAAILNTREPDVIIGSFPADHVIKTSPLFQQSVAQAVVAANEGYVVTIAITPTEPATQFGYIL
CGDSAGIKGAPQVLSVKRFVEKPSQKDADRYLSSGKYLWNAGMFIAKACVLLEQLEKARPDIHSLIIDIANSPDLDSALK
KNWPKMPRIAIEYSVAEPCAAEGRMVTVPAKFEWNDAGDFSSIARLNSRGGSDLVILGETSRVLSDSATGIVVSQTDRII
SVIGTKDIVVVDTPDSLLVTTKDDAKKVKQIVEALRLSGRDDVL

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group C2 O antigen [H]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=370, Percent_Identity=33.2432432432432, Blast_Score=171, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 39204; Mature: 39204

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDCSELYSVIPAGGIGSRLWPLSRAASPKFLHKLTGTETSLLRDTWDRLVPISGVDRIMV
CCHHHHHHHCCCCCCCCCCCCCHHCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEE
VIGSDHFDAVTSQLPELKGHNVILEPEPKDSTAAICLAAAILNTREPDVIIGSFPADHVI
EECCCHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCEE
KTSPLFQQSVAQAVVAANEGYVVTIAITPTEPATQFGYILCGDSAGIKGAPQVLSVKRFV
ECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHH
EKPSQKDADRYLSSGKYLWNAGMFIAKACVLLEQLEKARPDIHSLIIDIANSPDLDSALK
HCCCCCHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHH
KNWPKMPRIAIEYSVAEPCAAEGRMVTVPAKFEWNDAGDFSSIARLNSRGGSDLVILGET
HCCCCCCEEEEEEECCCCCCCCCEEEEECCEECCCCCCCHHHHHHHHCCCCCEEEEECCC
SRVLSDSATGIVVSQTDRIISVIGTKDIVVVDTPDSLLVTTKDDAKKVKQIVEALRLSGR
HHHHCCCCCEEEEECCCCEEEEECCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHCCCC
DDVL
CCCC
>Mature Secondary Structure
MDCSELYSVIPAGGIGSRLWPLSRAASPKFLHKLTGTETSLLRDTWDRLVPISGVDRIMV
CCHHHHHHHCCCCCCCCCCCCCHHCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEE
VIGSDHFDAVTSQLPELKGHNVILEPEPKDSTAAICLAAAILNTREPDVIIGSFPADHVI
EECCCHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCEE
KTSPLFQQSVAQAVVAANEGYVVTIAITPTEPATQFGYILCGDSAGIKGAPQVLSVKRFV
ECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHH
EKPSQKDADRYLSSGKYLWNAGMFIAKACVLLEQLEKARPDIHSLIIDIANSPDLDSALK
HCCCCCHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHH
KNWPKMPRIAIEYSVAEPCAAEGRMVTVPAKFEWNDAGDFSSIARLNSRGGSDLVILGET
HCCCCCCEEEEEEECCCCCCCCCEEEEECCEECCCCCCCHHHHHHHHCCCCCEEEEECCC
SRVLSDSATGIVVSQTDRIISVIGTKDIVVVDTPDSLLVTTKDDAKKVKQIVEALRLSGR
HHHHCCCCCEEEEECCCCEEEEECCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHCCCC
DDVL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379320 [H]