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The map label for this gene is frr

Identifier: 28572476

GI number: 28572476

Start: 353710

End: 354255

Strand: Direct

Name: frr

Synonym: TW321

Alternate gene names: 28572476

Gene position: 353710-354255 (Clockwise)

Preceding gene: 28572475

Following gene: 28572477

Centisome position: 38.2

GC content: 46.34

Gene sequence:

>546_bases
GTGGGCATCATGTGTAGCGGCGATGTGTCAGAGCGAATGAACAAAACAATTGAGCTTCTCAAGGAGCAGTTTTTGTCCAT
ACACAGTGGCAGGGTAAATTCAGGACAATTCGAAAAAGTTATGGTTGATTGCGAGGGCGCTAGCGTTCCTCTGGTTAGCC
TCGCGAGCATTAGGGTTTTGAATGCTAACACGATTGTCGTTACTCCATACGATTCAGCGCTTCTTTCACAGATTGACCGT
GCACTTCGTAATGTACCGAATATTGGCACTCCCGGAAATGATGGGGAGTGCATAAAGATTGTCATGCCACAGCTAACAGA
GGCGCGCAGACACGAATATGTAAAACAGGCTCGGGTGAAAGCAGAAGAGGCGAGGGTTTCAGCTAGGAATATACGCCGCA
AGGCTAGGGCAAGCCTTGATGCTATGGGACTGGCAAAGGATGAAATTGTCCGTCGTGAAAAGGAATTAGACAAACTAACT
AAGGATGTTATATCCGTAGTAGATGATTTGTTGAGACATAAGGAGTCTGAGCTTCTTAGGCTTTGA

Upstream 100 bases:

>100_bases
GGGTGTGATTTTGAGATAAGTGGGTTGTAAGTTTTGTTACGCTTAACTGTGTGCTGCCCCTTCTGGGCAGCTTGTCGAGC
TTTGATGGTTTGGATCTTAG

Downstream 100 bases:

>100_bases
TCCAGCCCCAAGACTTATCCGCGAATCTGTAGTGTTTGAATGAGGCTGCAATGCTTAGATATTTCTTCATGCTAAAACAA
AGGGTACAGGCCCGCACGGG

Product: ribosome recycling factor

Products: NA

Alternate protein names: RRF; Ribosome-releasing factor

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MGIMCSGDVSERMNKTIELLKEQFLSIHSGRVNSGQFEKVMVDCEGASVPLVSLASIRVLNANTIVVTPYDSALLSQIDR
ALRNVPNIGTPGNDGECIKIVMPQLTEARRHEYVKQARVKAEEARVSARNIRRKARASLDAMGLAKDEIVRREKELDKLT
KDVISVVDDLLRHKESELLRL

Sequences:

>Translated_181_residues
MGIMCSGDVSERMNKTIELLKEQFLSIHSGRVNSGQFEKVMVDCEGASVPLVSLASIRVLNANTIVVTPYDSALLSQIDR
ALRNVPNIGTPGNDGECIKIVMPQLTEARRHEYVKQARVKAEEARVSARNIRRKARASLDAMGLAKDEIVRREKELDKLT
KDVISVVDDLLRHKESELLRL
>Mature_180_residues
GIMCSGDVSERMNKTIELLKEQFLSIHSGRVNSGQFEKVMVDCEGASVPLVSLASIRVLNANTIVVTPYDSALLSQIDRA
LRNVPNIGTPGNDGECIKIVMPQLTEARRHEYVKQARVKAEEARVSARNIRRKARASLDAMGLAKDEIVRREKELDKLTK
DVISVVDDLLRHKESELLRL

Specific function: Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another

COG id: COG0233

COG function: function code J; Ribosome recycling factor

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RRF family

Homologues:

Organism=Escherichia coli, GI1786368, Length=179, Percent_Identity=31.2849162011173, Blast_Score=91, Evalue=6e-20,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): RRF_TROW8 (Q83HZ5)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789256.1
- ProteinModelPortal:   Q83HZ5
- SMR:   Q83HZ5
- STRING:   Q83HZ5
- GeneID:   1064609
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW321
- eggNOG:   COG0233
- HOGENOM:   HBG533975
- OMA:   YYGAETP
- ProtClustDB:   CLSK229652
- BioCyc:   TWHI218496:TW0301-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00040
- InterPro:   IPR002661
- InterPro:   IPR015998
- PANTHER:   PTHR20982
- PANTHER:   PTHR20982:SF3

Pfam domain/function: PF01765 RRF; SSF55194 Ribosome_recyc_fac

EC number: NA

Molecular weight: Translated: 20209; Mature: 20078

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIMCSGDVSERMNKTIELLKEQFLSIHSGRVNSGQFEKVMVDCEGASVPLVSLASIRVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHEEEE
NANTIVVTPYDSALLSQIDRALRNVPNIGTPGNDGECIKIVMPQLTEARRHEYVKQARVK
CCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
AEEARVSARNIRRKARASLDAMGLAKDEIVRREKELDKLTKDVISVVDDLLRHKESELLR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
L
C
>Mature Secondary Structure 
GIMCSGDVSERMNKTIELLKEQFLSIHSGRVNSGQFEKVMVDCEGASVPLVSLASIRVL
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHEEEE
NANTIVVTPYDSALLSQIDRALRNVPNIGTPGNDGECIKIVMPQLTEARRHEYVKQARVK
CCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
AEEARVSARNIRRKARASLDAMGLAKDEIVRREKELDKLTKDVISVVDDLLRHKESELLR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174