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The map label for this gene is ogt [H]

Identifier: 28572456

GI number: 28572456

Start: 337674

End: 338228

Strand: Reverse

Name: ogt [H]

Synonym: TW300

Alternate gene names: 28572456

Gene position: 338228-337674 (Counterclockwise)

Preceding gene: 28572457

Following gene: 28572442

Centisome position: 36.53

GC content: 41.8

Gene sequence:

>555_bases
ATGTCTTCAGTGGAATTTGCAGTACGCATGAGAGCCTGTGCTTTTAGGGTAAAGGTTTATTCTTCGCAAACACATATCAT
TAGGGTAAAAATCTTTCGGTATGCAAGTGTGATAGATGATGCACATAAAAATACGAGATATAAAACCAGGTCGCTTTCGG
AGGGTTCATGTGATTGTCATGCGAGCAATAAGAACTCATCTTTGACCACAGATTTAACCGCAGAGGCAAGGCGGGAAATC
CTCGCATATTTCTCAGGAACTCTAAAGACATTTTCGGTGCCCATAAGCCTGGAAAAGGCATCTGCAAAACAGGCAGATGT
TCTCAATATGCTTTTAAAAATTCCATACGGTGGATGTGTAAGCTACGGTGAGATAGCATCAAAAGTAAACTCCTCACCAA
GGGCTGTTGGTGCTTATTGCGCAAAGAATCCCCTGCCGTTTTTTGTACCGTGTCACAGGGTTATACGGTCAAATGGTGAG
GTGGGAGGGTATTGTGGGTCTTCAAATAATTTGATAAAGCACTCACTGATCGAAATTGAACAGAAAAACTCATAA

Upstream 100 bases:

>100_bases
CAATCTGTGAAAGCTAAGATGGTTGAAATACCCGAGCGCGTACGGAAACTCATAAACGAAATCGAGATGCAGGAAACGTC
CTTTAGGCAAGCAGTCTAGT

Downstream 100 bases:

>100_bases
AAGATAACTCAGTGGCACTAATAACACTCTGGGTCGTAATGGGCATAGCGGTAATGAGCATATCTGTATTCAATAACAGA
TGCAATTGATTACAGATATC

Product: DNA-binding/repair enzyme

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MSSVEFAVRMRACAFRVKVYSSQTHIIRVKIFRYASVIDDAHKNTRYKTRSLSEGSCDCHASNKNSSLTTDLTAEARREI
LAYFSGTLKTFSVPISLEKASAKQADVLNMLLKIPYGGCVSYGEIASKVNSSPRAVGAYCAKNPLPFFVPCHRVIRSNGE
VGGYCGSSNNLIKHSLIEIEQKNS

Sequences:

>Translated_184_residues
MSSVEFAVRMRACAFRVKVYSSQTHIIRVKIFRYASVIDDAHKNTRYKTRSLSEGSCDCHASNKNSSLTTDLTAEARREI
LAYFSGTLKTFSVPISLEKASAKQADVLNMLLKIPYGGCVSYGEIASKVNSSPRAVGAYCAKNPLPFFVPCHRVIRSNGE
VGGYCGSSNNLIKHSLIEIEQKNS
>Mature_183_residues
SSVEFAVRMRACAFRVKVYSSQTHIIRVKIFRYASVIDDAHKNTRYKTRSLSEGSCDCHASNKNSSLTTDLTAEARREIL
AYFSGTLKTFSVPISLEKASAKQADVLNMLLKIPYGGCVSYGEIASKVNSSPRAVGAYCAKNPLPFFVPCHRVIRSNGEV
GGYCGSSNNLIKHSLIEIEQKNS

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20251; Mature: 20120

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVEFAVRMRACAFRVKVYSSQTHIIRVKIFRYASVIDDAHKNTRYKTRSLSEGSCDCH
CCCHHHHHHHHHHHEEEEEECCCCEEEEEEHHHHHHHHHHHHCCCCEEECCCCCCCCCEE
ASNKNSSLTTDLTAEARREILAYFSGTLKTFSVPISLEKASAKQADVLNMLLKIPYGGCV
CCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEECEEECCCCCHHHHHHHHHHHCCCCCCC
SYGEIASKVNSSPRAVGAYCAKNPLPFFVPCHRVIRSNGEVGGYCGSSNNLIKHSLIEIE
CHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHEE
QKNS
CCCC
>Mature Secondary Structure 
SSVEFAVRMRACAFRVKVYSSQTHIIRVKIFRYASVIDDAHKNTRYKTRSLSEGSCDCH
CCHHHHHHHHHHHEEEEEECCCCEEEEEEHHHHHHHHHHHHCCCCEEECCCCCCCCCEE
ASNKNSSLTTDLTAEARREILAYFSGTLKTFSVPISLEKASAKQADVLNMLLKIPYGGCV
CCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEECEEECCCCCHHHHHHHHHHHCCCCCCC
SYGEIASKVNSSPRAVGAYCAKNPLPFFVPCHRVIRSNGEVGGYCGSSNNLIKHSLIEIE
CHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHEE
QKNS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2505068; 2506524; 9384377 [H]