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The map label for this gene is phoR [H]

Identifier: 28572225

GI number: 28572225

Start: 44860

End: 46251

Strand: Reverse

Name: phoR [H]

Synonym: TW054

Alternate gene names: 28572225

Gene position: 46251-44860 (Counterclockwise)

Preceding gene: 28572226

Following gene: 28572215

Centisome position: 5.0

GC content: 44.76

Gene sequence:

>1392_bases
GTGGTAACGCTTGTAGCGCTTGTGCTTGCATTTTCGTTCACTGCTGTGGGTATTGGAACCACTCTGGTTATAAAGAACGA
GATGATAAAAACAATTGATGAGCAGCTAATTCATATAAGCGGTACATTTCCGCTGTTTGAAAGCAGTTTTGAAGTGTTCA
GTCCGGGAAGGGCAAGCTACAAAGCTCATATTTATAAATTCGACGGGACACTGATTTGGCCTATCGGAGAAAACCCTTCT
ATCGAGTTTGACAGGAAAATTGACATTACACTTTACAACGAAAAGGGTAACACGCCGTTTACTTTGCACTCGATATCGAG
ACAAAAGAAATATAGAATGCTCTTGCAGCCACTGGAAACGGGCAGTGGGCAAGAAATCCTTGCTTATTACCTGCCGCAGT
CTGGTACTGAAAATACCGCCAATTTTTATCAAAGAAGCTATATTTGGTTTAGTCTTGCGGTATTTGCAATCGCTGCGTAC
ATAGTTTATCTAATAACCGCACATACACTATCTCCACTATATGCTCTTGAGAAGACCGCAAGGGCGTTTGCAAGAGGGGA
TTATTCAAGGCGGATAAGGTCTGAATATGTTTCTGCAGAAATACTCAATCTAAACAAATCTCTGAATTCCATGCTGGACA
GTCTCGAAAAAGCCCTTGGTGAGCGAGATAAAACACTTGGCGAGATGAAGCAATTCTTGGCAGATGCAAGTCACGAATTG
CGAACGCCACTTGTATCTCTTAGGGGTTATGCCGAGCTCTATAGGATTGGCGCCCTTAAGGGTAAGGAGGATATTGACAA
CGCGATTGAGCGGATTGAAAAAGAGGCAATACGAATGGGCACAATGGTTGAAGACTTGCTGTCTCTGACGCGCCTTGAAA
AGGCTACAGACCTTGTGCCTGTCGATGTGAAATATCTTGTCCAAGAGTCAGTTATTGACATGAATGTCCTGGAAAAAACT
CGTAAGATCACAACCGGTATTCCGAAAAGGCAATCCTGTATGATTCTCGGGAACGAAAATATGCTTCGGCGGTTACTTGC
AAATCTGATTAACAATGCCCTGCACTATACCCCGCCAGGCTCTCCAATAGAGATTGTGTTACGCACTGATAAAACAGATG
TTGTGATTGATGTGCGCGACCATGGTCCGGGCGTGCCAAGACAGCTACACGATAAAGTGTTCAAGCGGTTTTGGCGATCT
GACGCATCGCGGGCACGGAACAGCGGTGGTGTGGGGCTTGGGCTTTCTATTGTGGCATCTATTGTAAAAGTCCATAAAGG
CACAATAGAGATATTGCGCACACGAGGTGGTGGCGCTACATTTAGAACCAGGATTCCCAAGCTTGTTAATAAACACCGTT
CTGGATCCTCTAGTCATAGTCTCACACGGTGA

Upstream 100 bases:

>100_bases
GCGAGGTTTTGGTTATATCATGAGAACGCGCTGACTCGTGTCAGCAAAGTTTCCCAGTAAGCTGTACAGACTCGTTGCAC
GCTCTTCGATAAAGAGTAAG

Downstream 100 bases:

>100_bases
TAAGAAAAATCTTGCACCGCACAGCACGATTCGATACAGCACGATTCGATACAGCACGATTCGATACAGCACGATTCGAT
ACAGCACGATTCGATACAGC

Product: two component system sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MVTLVALVLAFSFTAVGIGTTLVIKNEMIKTIDEQLIHISGTFPLFESSFEVFSPGRASYKAHIYKFDGTLIWPIGENPS
IEFDRKIDITLYNEKGNTPFTLHSISRQKKYRMLLQPLETGSGQEILAYYLPQSGTENTANFYQRSYIWFSLAVFAIAAY
IVYLITAHTLSPLYALEKTARAFARGDYSRRIRSEYVSAEILNLNKSLNSMLDSLEKALGERDKTLGEMKQFLADASHEL
RTPLVSLRGYAELYRIGALKGKEDIDNAIERIEKEAIRMGTMVEDLLSLTRLEKATDLVPVDVKYLVQESVIDMNVLEKT
RKITTGIPKRQSCMILGNENMLRRLLANLINNALHYTPPGSPIEIVLRTDKTDVVIDVRDHGPGVPRQLHDKVFKRFWRS
DASRARNSGGVGLGLSIVASIVKVHKGTIEILRTRGGGATFRTRIPKLVNKHRSGSSSHSLTR

Sequences:

>Translated_463_residues
MVTLVALVLAFSFTAVGIGTTLVIKNEMIKTIDEQLIHISGTFPLFESSFEVFSPGRASYKAHIYKFDGTLIWPIGENPS
IEFDRKIDITLYNEKGNTPFTLHSISRQKKYRMLLQPLETGSGQEILAYYLPQSGTENTANFYQRSYIWFSLAVFAIAAY
IVYLITAHTLSPLYALEKTARAFARGDYSRRIRSEYVSAEILNLNKSLNSMLDSLEKALGERDKTLGEMKQFLADASHEL
RTPLVSLRGYAELYRIGALKGKEDIDNAIERIEKEAIRMGTMVEDLLSLTRLEKATDLVPVDVKYLVQESVIDMNVLEKT
RKITTGIPKRQSCMILGNENMLRRLLANLINNALHYTPPGSPIEIVLRTDKTDVVIDVRDHGPGVPRQLHDKVFKRFWRS
DASRARNSGGVGLGLSIVASIVKVHKGTIEILRTRGGGATFRTRIPKLVNKHRSGSSSHSLTR
>Mature_463_residues
MVTLVALVLAFSFTAVGIGTTLVIKNEMIKTIDEQLIHISGTFPLFESSFEVFSPGRASYKAHIYKFDGTLIWPIGENPS
IEFDRKIDITLYNEKGNTPFTLHSISRQKKYRMLLQPLETGSGQEILAYYLPQSGTENTANFYQRSYIWFSLAVFAIAAY
IVYLITAHTLSPLYALEKTARAFARGDYSRRIRSEYVSAEILNLNKSLNSMLDSLEKALGERDKTLGEMKQFLADASHEL
RTPLVSLRGYAELYRIGALKGKEDIDNAIERIEKEAIRMGTMVEDLLSLTRLEKATDLVPVDVKYLVQESVIDMNVLEKT
RKITTGIPKRQSCMILGNENMLRRLLANLINNALHYTPPGSPIEIVLRTDKTDVVIDVRDHGPGVPRQLHDKVFKRFWRS
DASRARNSGGVGLGLSIVASIVKVHKGTIEILRTRGGGATFRTRIPKLVNKHRSGSSSHSLTR

Specific function: Member of the two-component regulatory system phoP/phoR involved in the alkaline phosphatase genes regulation. PhoR may function as a membrane-associated protein kinase that phosphorylates phoP in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790346, Length=279, Percent_Identity=31.8996415770609, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1786600, Length=332, Percent_Identity=28.9156626506024, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1786783, Length=295, Percent_Identity=28.4745762711864, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788393, Length=280, Percent_Identity=29.6428571428571, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787894, Length=301, Percent_Identity=27.2425249169435, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788279, Length=314, Percent_Identity=29.6178343949045, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790861, Length=250, Percent_Identity=28, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1789149, Length=347, Percent_Identity=24.4956772334294, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786912, Length=221, Percent_Identity=31.6742081447964, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790551, Length=288, Percent_Identity=30.5555555555556, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1789403, Length=291, Percent_Identity=28.5223367697594, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693157, Length=263, Percent_Identity=26.615969581749, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789808, Length=302, Percent_Identity=26.4900662251656, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081816, Length=260, Percent_Identity=26.5384615384615, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI48994928, Length=239, Percent_Identity=27.1966527196653, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788713, Length=246, Percent_Identity=25.609756097561, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87082128, Length=314, Percent_Identity=25.4777070063694, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=28.1938325991189, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788549, Length=240, Percent_Identity=26.25, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787374, Length=300, Percent_Identity=22.6666666666667, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52011; Mature: 52011

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTLVALVLAFSFTAVGIGTTLVIKNEMIKTIDEQLIHISGTFPLFESSFEVFSPGRASY
CHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHEEECCCCCHHHCCCHHCCCCCCCC
KAHIYKFDGTLIWPIGENPSIEFDRKIDITLYNEKGNTPFTLHSISRQKKYRMLLQPLET
EEEEEEECCEEEEECCCCCCEEECCEEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCC
GSGQEILAYYLPQSGTENTANFYQRSYIWFSLAVFAIAAYIVYLITAHTLSPLYALEKTA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAFARGDYSRRIRSEYVSAEILNLNKSLNSMLDSLEKALGERDKTLGEMKQFLADASHEL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHH
RTPLVSLRGYAELYRIGALKGKEDIDNAIERIEKEAIRMGTMVEDLLSLTRLEKATDLVP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VDVKYLVQESVIDMNVLEKTRKITTGIPKRQSCMILGNENMLRRLLANLINNALHYTPPG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCEECCCCC
SPIEIVLRTDKTDVVIDVRDHGPGVPRQLHDKVFKRFWRSDASRARNSGGVGLGLSIVAS
CCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
IVKVHKGTIEILRTRGGGATFRTRIPKLVNKHRSGSSSHSLTR
HHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MVTLVALVLAFSFTAVGIGTTLVIKNEMIKTIDEQLIHISGTFPLFESSFEVFSPGRASY
CHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHEEECCCCCHHHCCCHHCCCCCCCC
KAHIYKFDGTLIWPIGENPSIEFDRKIDITLYNEKGNTPFTLHSISRQKKYRMLLQPLET
EEEEEEECCEEEEECCCCCCEEECCEEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCC
GSGQEILAYYLPQSGTENTANFYQRSYIWFSLAVFAIAAYIVYLITAHTLSPLYALEKTA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAFARGDYSRRIRSEYVSAEILNLNKSLNSMLDSLEKALGERDKTLGEMKQFLADASHEL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHH
RTPLVSLRGYAELYRIGALKGKEDIDNAIERIEKEAIRMGTMVEDLLSLTRLEKATDLVP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VDVKYLVQESVIDMNVLEKTRKITTGIPKRQSCMILGNENMLRRLLANLINNALHYTPPG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCEECCCCC
SPIEIVLRTDKTDVVIDVRDHGPGVPRQLHDKVFKRFWRSDASRARNSGGVGLGLSIVAS
CCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
IVKVHKGTIEILRTRGGGATFRTRIPKLVNKHRSGSSSHSLTR
HHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3142862; 9387221; 9384377 [H]