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The map label for this gene is pflA2 [H]

Identifier: 28379672

GI number: 28379672

Start: 2951354

End: 2952175

Strand: Direct

Name: pflA2 [H]

Synonym: lp_3314

Alternate gene names: 28379672

Gene position: 2951354-2952175 (Clockwise)

Preceding gene: 28379671

Following gene: 28379674

Centisome position: 89.21

GC content: 46.23

Gene sequence:

>822_bases
ATGGAAAACAAACAAGTTTCAACAACGCAAGCGGCGGCAAAGGAGCCTTTGATAGGCTACGTTCACTCCATCGAAACGTT
TGGCTCCGTTGACGGACCAGGTATCCGTTACGTGGCATTCCTTCAAGGATGCCACATGCGTTGCCAATACTGTCACAACC
CTGATACTTGGAAACTCAACGTTGGCGATCAAATGACGGCCGACGAGATTCTCGAAGACGCGGCTAAATACCGGGCTTTC
TGGGGCAAGACGGGTGGCATCACAGTCAGTGGTGGTGAATCACTGGTACAAATCGACTTCATCTTAGACTTATTCGAAAA
AGCCAAGGCGATGAATATCAGTACTTGTCTGGATACCTCTGGACAGCCTTTTACCCGAGAACAACCTTTCTTTGACAAGT
TCGAACGTCTAATGAAGGTCACGGACATTTCGTTGGTCGACATTAAGCACATCGATTCTGCCAAACACAAGCAGTTGACC
CAGTATGGGAACGAAAATATCTTGGATATGATTCAGTACATGGCCCAACACCACGATGATATGTGGATTCGTCACGTCCT
GGTTCCCCAACGGACTGATTACGATGAAGACTTGAAGAAACTCGGCGATTACATTGCTAAAATTCCAAACGACGTCGTTC
AAAAAGTCGAAGTATTGCCGTACCATACTTTGGGCGTTAAAAAATATCATGAAATGAAGATCAAGTACCGGCTTGAAGGA
ATCGAGTCTCCAACCCAAGATCGGGTGGCAAATGCCGAAAAGCTACTGCACACTGCTGATTACAACGGGTACAAGACATG
GATGCCATTGCCAAAACTTTAA

Upstream 100 bases:

>100_bases
CGCAGATTTAACCAAGGAACAACAAGATGACGTTATTTCACGGACATTCTTCGAATCAATGTAACATTTAATCTTTAATA
GTTAAGAAGGCGAAAGGCTC

Downstream 100 bases:

>100_bases
ATCGGCATTTCTAGCATGACTAAAACACCAGCGATACAATCGTCTACGGATGGTTGTGTCACTGGTGTTTTTTTGCTGGC
TCGACGCATACACGTATTGC

Product: formate acetyltransferase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MENKQVSTTQAAAKEPLIGYVHSIETFGSVDGPGIRYVAFLQGCHMRCQYCHNPDTWKLNVGDQMTADEILEDAAKYRAF
WGKTGGITVSGGESLVQIDFILDLFEKAKAMNISTCLDTSGQPFTREQPFFDKFERLMKVTDISLVDIKHIDSAKHKQLT
QYGNENILDMIQYMAQHHDDMWIRHVLVPQRTDYDEDLKKLGDYIAKIPNDVVQKVEVLPYHTLGVKKYHEMKIKYRLEG
IESPTQDRVANAEKLLHTADYNGYKTWMPLPKL

Sequences:

>Translated_273_residues
MENKQVSTTQAAAKEPLIGYVHSIETFGSVDGPGIRYVAFLQGCHMRCQYCHNPDTWKLNVGDQMTADEILEDAAKYRAF
WGKTGGITVSGGESLVQIDFILDLFEKAKAMNISTCLDTSGQPFTREQPFFDKFERLMKVTDISLVDIKHIDSAKHKQLT
QYGNENILDMIQYMAQHHDDMWIRHVLVPQRTDYDEDLKKLGDYIAKIPNDVVQKVEVLPYHTLGVKKYHEMKIKYRLEG
IESPTQDRVANAEKLLHTADYNGYKTWMPLPKL
>Mature_273_residues
MENKQVSTTQAAAKEPLIGYVHSIETFGSVDGPGIRYVAFLQGCHMRCQYCHNPDTWKLNVGDQMTADEILEDAAKYRAF
WGKTGGITVSGGESLVQIDFILDLFEKAKAMNISTCLDTSGQPFTREQPFFDKFERLMKVTDISLVDIKHIDSAKHKQLT
QYGNENILDMIQYMAQHHDDMWIRHVLVPQRTDYDEDLKKLGDYIAKIPNDVVQKVEVLPYHTLGVKKYHEMKIKYRLEG
IESPTQDRVANAEKLLHTADYNGYKTWMPLPKL

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=243, Percent_Identity=38.6831275720165, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1790839, Length=294, Percent_Identity=27.891156462585, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790389, Length=289, Percent_Identity=21.1072664359862, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 31319; Mature: 31319

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKQVSTTQAAAKEPLIGYVHSIETFGSVDGPGIRYVAFLQGCHMRCQYCHNPDTWKLN
CCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
VGDQMTADEILEDAAKYRAFWGKTGGITVSGGESLVQIDFILDLFEKAKAMNISTCLDTS
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCEEHHHHHHHHHHHHHHCCHHHHHCCC
GQPFTREQPFFDKFERLMKVTDISLVDIKHIDSAKHKQLTQYGNENILDMIQYMAQHHDD
CCCCCCCCCHHHHHHHHHHHHCCCEEEHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCCC
MWIRHVLVPQRTDYDEDLKKLGDYIAKIPNDVVQKVEVLPYHTLGVKKYHEMKIKYRLEG
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHCHHHHHHHEEEEEECC
IESPTQDRVANAEKLLHTADYNGYKTWMPLPKL
CCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCC
>Mature Secondary Structure
MENKQVSTTQAAAKEPLIGYVHSIETFGSVDGPGIRYVAFLQGCHMRCQYCHNPDTWKLN
CCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
VGDQMTADEILEDAAKYRAFWGKTGGITVSGGESLVQIDFILDLFEKAKAMNISTCLDTS
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCEEHHHHHHHHHHHHHHCCHHHHHCCC
GQPFTREQPFFDKFERLMKVTDISLVDIKHIDSAKHKQLTQYGNENILDMIQYMAQHHDD
CCCCCCCCCHHHHHHHHHHHHCCCEEEHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCCC
MWIRHVLVPQRTDYDEDLKKLGDYIAKIPNDVVQKVEVLPYHTLGVKKYHEMKIKYRLEG
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHCHHHHHHHEEEEEECC
IESPTQDRVANAEKLLHTADYNGYKTWMPLPKL
CCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11029425; 10899886; 12397186 [H]