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The map label for this gene is pflB2 [H]

Identifier: 28379671

GI number: 28379671

Start: 2949059

End: 2951317

Strand: Direct

Name: pflB2 [H]

Synonym: lp_3313

Alternate gene names: 28379671

Gene position: 2949059-2951317 (Clockwise)

Preceding gene: 28379669

Following gene: 28379672

Centisome position: 89.14

GC content: 43.96

Gene sequence:

>2259_bases
ATGATTACATCAGAAAAGACAACAAAACCAGCAGCTTGGAAAGGTTTCAAAGGCGGGCACTGGCAGGAAGAAATCAACAT
TCGTGATTTTATTCAAAATAACTTCACACAGTACAATGGCGACGAAAGCTTCCTGGCCGGACCAACAGCCGCTACTAAGA
CCTTGAATGACAAAGTCTTAGAATTAAAGAAACAAGAACGTGCCGCTGGTGGTGTGTTGGATGCTGATACTAAAGTCGTT
GCAACGATTACTTCACACGGCCCTGGTTATATTCAAAAAGATCTCGAAAAGATTGTTGGTCTCCAGACTGACAAGCCTTT
GAAGCGGGCCTTCATGCCATTTGGTGGTATTCGAATGGCTGATGACGCTTTGAAATCATACGGTTATACCCCTGATGAAG
AAAACGACAAGATTTTCACTGAATATCGCAAGACTCATAACCAAGGCGTCTTCGATGTTTATACTCCTGACATGCGGAAA
GCACGTCACTACAAGATCATCACCGGACTACCAGATGCATACGCACGTGGCCGTCTCATTCCTGATCTTCCACGGGTCGC
TGTTTATGGGATCGATCGTTTAATGGAAGACAAAGCTAATGACTTTGCCCACATTGGTGATGGTGAATTGACTGATGATG
TTATTCGCCTCCGTGAAGAAGTTCAAGATCAATACCGTGCTTTAGCAGATATGAAGAAGATGGCTGCCAGTTATGGCTAC
GATATTAGCAAGCCTGCAACTAATGCTCAAGAAGCTATTCAATGGATGTACTTCGCTTACTTAGCTGCTATCAAGACCCA
AAACGGCGCTGCAATGTCCGTTGGCCGGATTGATACAACGATGGACATCTTCATCCAACGTGACTTGGACAATGGTGTTC
TGGACGAAAGCCAAGCTCAAGAATTAATTGATCAATTCGTCATGAAACTACGGATGGTTCGGTTCATCCGTACTGAAGAA
TACAATTCTCTCTTCTCTGGTGACCCAATCTGGGCAACCTTATCAATGTGTGGTTTAGGCGTCGACGGTCAACACCATGT
GACTAAGACTGCTTTCCGGATTTTAAAGACTTTGGACAACATGGGCGCCGCACCAGAACCAAACATCACGATTTTATGGT
CAGACCGCTTACCAGAAGACTTCAAACGTTACGCAACTGAAGTTTCAATCGACAGTTCAACCATTCAGTATGAAAATGAT
GACTTGATGCGGGTACAATGGGGTACCGATTATTATGGCATTGCTTGCTGTGTTTCCGCACAACCAATTGCTGATGGAAT
CCAGTACTTCGGTGCCCGGGCAAACTTAGCCAAAGCGATTCTTTATGCCATCAATGGTGGCCGCGACGAAATTGCTGGAG
ATCAAGTTGGCCCTGCTTACGAACCAATTACTTCAGAATACATCGATTACGACGAATTCATGAAGAAATTAGACAAGCAA
ATGGATTGGTTAGCTGACACTTACGTTAACTCACTGAATGCAATTCATTATATGCATGATAAGTACTACTATGAAGCTGC
CCAATTAGCTTTGAAGAATACTGATCTTGATCGGACCTTTGCAACTGGGATTTCTGGCTTATCACATGCCGCGGATTCAA
TCTCAGCTATCAAGTATGGTCACGTTAAAGTAATTCGTGACGAACGTGGTATCGCCGTTGACTTCAAAGCCGACAATGAC
TACCCACGTTATGGGAACAATGACGATCGCGCTGATGACATTGCTAAATGGTTAGTCAAAGAATTATACAGCAAGATGAA
CACGCATCACCTCTATCGGAATGCCAAACTTTCAACTTCTGTTTTGACGATTACCTCCAACGTTGTTTATGGTAAGAACA
CTGGTACCACGCCAAATGGCCGTCAAAAAGGCGAACCATTCTCACCAGGTGCTAACCCTGCATACGGTGCTGAAAAGAGT
GGTGCATTAGCTTCACTTCTTTCAACTGCCAAATTACCATACCGTTACGCAACTGACGGGATTTCCAACACGTTCGGCGT
TACCCCTAACACGTTAGGCCATGACCTCGAATCACGGAAAGACACGTTAGTAAACATGTTAGACGGTTACATGAAGAACG
ATGGGATGCACTTGAACATCAACGTCTTCAATAAAGACACTTTGATTGATGCTCAGAAACACCCTGAAGAATACCCAACA
TTAACGGTTCGGGTTTCTGGCTATTGTGTCTACTTCGCAGATTTAACCAAGGAACAACAAGATGACGTTATTTCACGGAC
ATTCTTCGAATCAATGTAA

Upstream 100 bases:

>100_bases
AATCACCTGAAAGCGGTTACAAATCCAGCAAAAAATGTTATGATTCAAATATAGAATTTTATCAATCATTTGATTGGATT
CAAATTATGGAGGCGAGAAA

Downstream 100 bases:

>100_bases
CATTTAATCTTTAATAGTTAAGAAGGCGAAAGGCTCATGGAAAACAAACAAGTTTCAACAACGCAAGCGGCGGCAAAGGA
GCCTTTGATAGGCTACGTTC

Product: formate C-acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 752; Mature: 752

Protein sequence:

>752_residues
MITSEKTTKPAAWKGFKGGHWQEEINIRDFIQNNFTQYNGDESFLAGPTAATKTLNDKVLELKKQERAAGGVLDADTKVV
ATITSHGPGYIQKDLEKIVGLQTDKPLKRAFMPFGGIRMADDALKSYGYTPDEENDKIFTEYRKTHNQGVFDVYTPDMRK
ARHYKIITGLPDAYARGRLIPDLPRVAVYGIDRLMEDKANDFAHIGDGELTDDVIRLREEVQDQYRALADMKKMAASYGY
DISKPATNAQEAIQWMYFAYLAAIKTQNGAAMSVGRIDTTMDIFIQRDLDNGVLDESQAQELIDQFVMKLRMVRFIRTEE
YNSLFSGDPIWATLSMCGLGVDGQHHVTKTAFRILKTLDNMGAAPEPNITILWSDRLPEDFKRYATEVSIDSSTIQYEND
DLMRVQWGTDYYGIACCVSAQPIADGIQYFGARANLAKAILYAINGGRDEIAGDQVGPAYEPITSEYIDYDEFMKKLDKQ
MDWLADTYVNSLNAIHYMHDKYYYEAAQLALKNTDLDRTFATGISGLSHAADSISAIKYGHVKVIRDERGIAVDFKADND
YPRYGNNDDRADDIAKWLVKELYSKMNTHHLYRNAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPAYGAEKS
GALASLLSTAKLPYRYATDGISNTFGVTPNTLGHDLESRKDTLVNMLDGYMKNDGMHLNINVFNKDTLIDAQKHPEEYPT
LTVRVSGYCVYFADLTKEQQDDVISRTFFESM

Sequences:

>Translated_752_residues
MITSEKTTKPAAWKGFKGGHWQEEINIRDFIQNNFTQYNGDESFLAGPTAATKTLNDKVLELKKQERAAGGVLDADTKVV
ATITSHGPGYIQKDLEKIVGLQTDKPLKRAFMPFGGIRMADDALKSYGYTPDEENDKIFTEYRKTHNQGVFDVYTPDMRK
ARHYKIITGLPDAYARGRLIPDLPRVAVYGIDRLMEDKANDFAHIGDGELTDDVIRLREEVQDQYRALADMKKMAASYGY
DISKPATNAQEAIQWMYFAYLAAIKTQNGAAMSVGRIDTTMDIFIQRDLDNGVLDESQAQELIDQFVMKLRMVRFIRTEE
YNSLFSGDPIWATLSMCGLGVDGQHHVTKTAFRILKTLDNMGAAPEPNITILWSDRLPEDFKRYATEVSIDSSTIQYEND
DLMRVQWGTDYYGIACCVSAQPIADGIQYFGARANLAKAILYAINGGRDEIAGDQVGPAYEPITSEYIDYDEFMKKLDKQ
MDWLADTYVNSLNAIHYMHDKYYYEAAQLALKNTDLDRTFATGISGLSHAADSISAIKYGHVKVIRDERGIAVDFKADND
YPRYGNNDDRADDIAKWLVKELYSKMNTHHLYRNAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPAYGAEKS
GALASLLSTAKLPYRYATDGISNTFGVTPNTLGHDLESRKDTLVNMLDGYMKNDGMHLNINVFNKDTLIDAQKHPEEYPT
LTVRVSGYCVYFADLTKEQQDDVISRTFFESM
>Mature_752_residues
MITSEKTTKPAAWKGFKGGHWQEEINIRDFIQNNFTQYNGDESFLAGPTAATKTLNDKVLELKKQERAAGGVLDADTKVV
ATITSHGPGYIQKDLEKIVGLQTDKPLKRAFMPFGGIRMADDALKSYGYTPDEENDKIFTEYRKTHNQGVFDVYTPDMRK
ARHYKIITGLPDAYARGRLIPDLPRVAVYGIDRLMEDKANDFAHIGDGELTDDVIRLREEVQDQYRALADMKKMAASYGY
DISKPATNAQEAIQWMYFAYLAAIKTQNGAAMSVGRIDTTMDIFIQRDLDNGVLDESQAQELIDQFVMKLRMVRFIRTEE
YNSLFSGDPIWATLSMCGLGVDGQHHVTKTAFRILKTLDNMGAAPEPNITILWSDRLPEDFKRYATEVSIDSSTIQYEND
DLMRVQWGTDYYGIACCVSAQPIADGIQYFGARANLAKAILYAINGGRDEIAGDQVGPAYEPITSEYIDYDEFMKKLDKQ
MDWLADTYVNSLNAIHYMHDKYYYEAAQLALKNTDLDRTFATGISGLSHAADSISAIKYGHVKVIRDERGIAVDFKADND
YPRYGNNDDRADDIAKWLVKELYSKMNTHHLYRNAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPAYGAEKS
GALASLLSTAKLPYRYATDGISNTFGVTPNTLGHDLESRKDTLVNMLDGYMKNDGMHLNINVFNKDTLIDAQKHPEEYPT
LTVRVSGYCVYFADLTKEQQDDVISRTFFESM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=750, Percent_Identity=59.4666666666667, Blast_Score=920, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=750, Percent_Identity=57.2, Blast_Score=882, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=582, Percent_Identity=25.7731958762887, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1790388, Length=625, Percent_Identity=22.88, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI1788933, Length=58, Percent_Identity=65.5172413793103, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84481; Mature: 84481

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSEKTTKPAAWKGFKGGHWQEEINIRDFIQNNFTQYNGDESFLAGPTAATKTLNDKVL
CCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHCCCCCCCCCCCEECCCCHHHHHHHHHHH
ELKKQERAAGGVLDADTKVVATITSHGPGYIQKDLEKIVGLQTDKPLKRAFMPFGGIRMA
HHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHH
DDALKSYGYTPDEENDKIFTEYRKTHNQGVFDVYTPDMRKARHYKIITGLPDAYARGRLI
HHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHCCCEEEEECCCCHHHCCCCC
PDLPRVAVYGIDRLMEDKANDFAHIGDGELTDDVIRLREEVQDQYRALADMKKMAASYGY
CCCHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DISKPATNAQEAIQWMYFAYLAAIKTQNGAAMSVGRIDTTMDIFIQRDLDNGVLDESQAQ
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEEECCCCCCCCHHHHH
ELIDQFVMKLRMVRFIRTEEYNSLFSGDPIWATLSMCGLGVDGQHHVTKTAFRILKTLDN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
MGAAPEPNITILWSDRLPEDFKRYATEVSIDSSTIQYENDDLMRVQWGTDYYGIACCVSA
CCCCCCCCEEEEECCCCCHHHHHHHHHEEECCCEEEECCCCEEEEEECCCCEEEEEEECC
QPIADGIQYFGARANLAKAILYAINGGRDEIAGDQVGPAYEPITSEYIDYDEFMKKLDKQ
CHHHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHH
MDWLADTYVNSLNAIHYMHDKYYYEAAQLALKNTDLDRTFATGISGLSHAADSISAIKYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEC
HVKVIRDERGIAVDFKADNDYPRYGNNDDRADDIAKWLVKELYSKMNTHHLYRNAKLSTS
CEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPAYGAEKSGALASLLSTAKLPYRYATDG
EEEEEECEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCC
ISNTFGVTPNTLGHDLESRKDTLVNMLDGYMKNDGMHLNINVFNKDTLIDAQKHPEEYPT
CCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEECCHHCCCCCCE
LTVRVSGYCVYFADLTKEQQDDVISRTFFESM
EEEEECCEEEEEEHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MITSEKTTKPAAWKGFKGGHWQEEINIRDFIQNNFTQYNGDESFLAGPTAATKTLNDKVL
CCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHCCCCCCCCCCCEECCCCHHHHHHHHHHH
ELKKQERAAGGVLDADTKVVATITSHGPGYIQKDLEKIVGLQTDKPLKRAFMPFGGIRMA
HHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHH
DDALKSYGYTPDEENDKIFTEYRKTHNQGVFDVYTPDMRKARHYKIITGLPDAYARGRLI
HHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHCCCEEEEECCCCHHHCCCCC
PDLPRVAVYGIDRLMEDKANDFAHIGDGELTDDVIRLREEVQDQYRALADMKKMAASYGY
CCCHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DISKPATNAQEAIQWMYFAYLAAIKTQNGAAMSVGRIDTTMDIFIQRDLDNGVLDESQAQ
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEEECCCCCCCCHHHHH
ELIDQFVMKLRMVRFIRTEEYNSLFSGDPIWATLSMCGLGVDGQHHVTKTAFRILKTLDN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
MGAAPEPNITILWSDRLPEDFKRYATEVSIDSSTIQYENDDLMRVQWGTDYYGIACCVSA
CCCCCCCCEEEEECCCCCHHHHHHHHHEEECCCEEEECCCCEEEEEECCCCEEEEEEECC
QPIADGIQYFGARANLAKAILYAINGGRDEIAGDQVGPAYEPITSEYIDYDEFMKKLDKQ
CHHHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHH
MDWLADTYVNSLNAIHYMHDKYYYEAAQLALKNTDLDRTFATGISGLSHAADSISAIKYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEC
HVKVIRDERGIAVDFKADNDYPRYGNNDDRADDIAKWLVKELYSKMNTHHLYRNAKLSTS
CEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPAYGAEKSGALASLLSTAKLPYRYATDG
EEEEEECEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCC
ISNTFGVTPNTLGHDLESRKDTLVNMLDGYMKNDGMHLNINVFNKDTLIDAQKHPEEYPT
CCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEECCHHCCCCCCE
LTVRVSGYCVYFADLTKEQQDDVISRTFFESM
EEEEECCEEEEEEHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA