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The map label for this gene is tag2 [H]

Identifier: 28379448

GI number: 28379448

Start: 2686705

End: 2687349

Strand: Direct

Name: tag2 [H]

Synonym: lp_3020

Alternate gene names: 28379448

Gene position: 2686705-2687349 (Clockwise)

Preceding gene: 28379447

Following gene: 28379449

Centisome position: 81.21

GC content: 48.06

Gene sequence:

>645_bases
ATGATTACCAACTGGTTTGATGATGATCCACTATTCAATCGTTACTATCCGTACGATGCCGGCTACCGGTATTACTTTGA
ACACGAATGGGGTATCCCGGTTCAGCGCGATGAACAATTATTTGAGTTTCTGAGTCTTGGCGGTTTTGCCGCTGGTCTCA
ACTGGGCCGTCGTGTTCAATAAACGGTCAGCATTCAGCGCAGCATTTGACGCCTGGCAAATTGAGGTGGTGGCAGCGTAT
CGCTCAGAACAAATCACCAATCTCCTCAGCAATTCGGCCATCATACGAAACCGTCGTAAAATCGAAGCCGTGATTCACAA
CGCTCGCCTGATTCAACAAATTCGGACCACACAGTCTTTTAAAGACTATCTCTGGCAACAATTGGGCCATCAACAATTAA
TTATCCAGCCCCAGTATTATGATGAACTCCCACGCACAACAATTACTGGCGATCGCATCGCACAGCACTTACAACAAGTT
GGTTTTAAATTTGTCGGTCCGGTAACGATTAATGCTTGGCTGGTAAACACCGGTTTTATTACGGCACGTCCCGACCAACT
GGGCATTATTACGACGACTCCCCGGCCTTCGTCAACGATGCTGCCCCCACGCTCACCGCACCAACTCGGGCATTTTAAGC
CTTAA

Upstream 100 bases:

>100_bases
CTCAGGAGGATTGGAAAAGTTTTTTTATCCACGTGTTTTCCGACCCATTTACCGTATTTAGTTAGTGTCTACTAATTGAT
TTGGAAATGAGGCTTGTTTA

Downstream 100 bases:

>100_bases
CCCAGGCTTGTTGCGCTGCTTTAATTGCCGTTCGGCGTATCTAGCGCGAGGCCCGCTGATTTTCCCTGATAATAACGGCT
AAAAAGCCAATTAAAGTCCA

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MITNWFDDDPLFNRYYPYDAGYRYYFEHEWGIPVQRDEQLFEFLSLGGFAAGLNWAVVFNKRSAFSAAFDAWQIEVVAAY
RSEQITNLLSNSAIIRNRRKIEAVIHNARLIQQIRTTQSFKDYLWQQLGHQQLIIQPQYYDELPRTTITGDRIAQHLQQV
GFKFVGPVTINAWLVNTGFITARPDQLGIITTTPRPSSTMLPPRSPHQLGHFKP

Sequences:

>Translated_214_residues
MITNWFDDDPLFNRYYPYDAGYRYYFEHEWGIPVQRDEQLFEFLSLGGFAAGLNWAVVFNKRSAFSAAFDAWQIEVVAAY
RSEQITNLLSNSAIIRNRRKIEAVIHNARLIQQIRTTQSFKDYLWQQLGHQQLIIQPQYYDELPRTTITGDRIAQHLQQV
GFKFVGPVTINAWLVNTGFITARPDQLGIITTTPRPSSTMLPPRSPHQLGHFKP
>Mature_214_residues
MITNWFDDDPLFNRYYPYDAGYRYYFEHEWGIPVQRDEQLFEFLSLGGFAAGLNWAVVFNKRSAFSAAFDAWQIEVVAAY
RSEQITNLLSNSAIIRNRRKIEAVIHNARLIQQIRTTQSFKDYLWQQLGHQQLIIQPQYYDELPRTTITGDRIAQHLQQV
GFKFVGPVTINAWLVNTGFITARPDQLGIITTTPRPSSTMLPPRSPHQLGHFKP

Specific function: Catalyzes the synthesis of GMP from XMP [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 N-acetyltransferase domains [H]

Homologues:

Organism=Escherichia coli, GI1789971, Length=179, Percent_Identity=32.9608938547486, Blast_Score=112, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR005019
- InterPro:   IPR001962
- InterPro:   IPR001317
- InterPro:   IPR011257
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF03352 Adenine_glyco; PF00733 Asn_synthase; PF00117 GATase; PF00958 GMP_synt_C [H]

EC number: =6.3.5.2 [H]

Molecular weight: Translated: 24850; Mature: 24850

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITNWFDDDPLFNRYYPYDAGYRYYFEHEWGIPVQRDEQLFEFLSLGGFAAGLNWAVVFN
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCHHCCCEEEEEEE
KRSAFSAAFDAWQIEVVAAYRSEQITNLLSNSAIIRNRRKIEAVIHNARLIQQIRTTQSF
CCCHHHHHHCCEEEEEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDYLWQQLGHQQLIIQPQYYDELPRTTITGDRIAQHLQQVGFKFVGPVTINAWLVNTGFI
HHHHHHHCCCCEEEECCHHHHHCCCCCCCHHHHHHHHHHCCCEEECCEEEEEEEEECCEE
TARPDQLGIITTTPRPSSTMLPPRSPHQLGHFKP
EECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MITNWFDDDPLFNRYYPYDAGYRYYFEHEWGIPVQRDEQLFEFLSLGGFAAGLNWAVVFN
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCHHCCCEEEEEEE
KRSAFSAAFDAWQIEVVAAYRSEQITNLLSNSAIIRNRRKIEAVIHNARLIQQIRTTQSF
CCCHHHHHHCCEEEEEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDYLWQQLGHQQLIIQPQYYDELPRTTITGDRIAQHLQQVGFKFVGPVTINAWLVNTGFI
HHHHHHHCCCCEEEECCHHHHHCCCCCCCHHHHHHHHHHCCCEEECCEEEEEEEEECCEE
TARPDQLGIITTTPRPSSTMLPPRSPHQLGHFKP
EECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954 [H]