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The map label for this gene is pbg3 [H]

Identifier: 28378133

GI number: 28378133

Start: 1284345

End: 1285817

Strand: Direct

Name: pbg3 [H]

Synonym: lp_1401

Alternate gene names: 28378133

Gene position: 1284345-1285817 (Clockwise)

Preceding gene: 28378132

Following gene: 28378138

Centisome position: 38.82

GC content: 44.33

Gene sequence:

>1473_bases
ATGCAATTTCCGGCTGATTTTTACTGGGGTGGGGCTACTGCCGCTAATCAATGCGAAGGTGCTTATGATGTTGATGGTCG
GGGCCTGACTATGAAGGATATTACGACAATGGGAGGCCTTAATCAGCGACGGCAAGTCACTTATTTGCAAGCGGATGGTA
CTCCTGGAAAAGGGGATTCAATTCCTGCTGGAGCACACGGAGCGGTACTGCCAGATGACTATTATCCTAATCAGACTAGT
ATTGACTTCTATCATCGATATCAAGAAGATATTGCGCTGTTTGCAGAGATGGGTTTCAAAATGTATCGAATGTCGATTTC
CTGGTCACGGATTTTCCCACGCGGAGATGAAAATGAACCTAATCAAGCAGGGTTGGATTTTTATCGCCGGGTGTTCGAAA
CTTTGAAAAAATATGAAATTGAACCATTGGTGACGATTTCACACTTCGATATGCCACTGTATCTGGAGGAAACGTATGGT
GGCTGGAATGACCGTCGAATGATTGGTTTTTACCAGCATTATGCGGAAACGCTATTTACGGCGTATCGTGGGCTGGTAAA
GCACTGGATTACCTTCAATGAAATTAATAATACAATCATGTTTCTGGCATTGCGTGCCAAAGCCGGTGATGCAGATTACC
AACGCGCTTATCAACAATTACATTATCAATTTGTTGCTAGTGCTTTAGCCGTACAGCAAGCACATGCGATTGATGGCGAA
AATAAAGTTGGTTGCATGATTTGCGGTATCACGTCTTATCCGTTAACCTGTGATCCGGCGGATGTCTTGCAGAATCGGTA
CGTCTGGGAACAAAACATCTATTACTGTGGTGATGTGCAGTGCCAGGGACAGTATCCAACCTATGCACGACGTTTATGGA
ATGAGCATCAAGTTGATTTAGACATTACGGACAGTGATTTAGAAGCCTTGAAAGCCGGTACGGTTGACTGGTATACCTTC
TCGTATTACATGTCAACGGCAGTTACAACACATGAGGTCAGTGCAACTGTTGGCGGTAATTTTTCGATGGGCGCGCGTAA
CCCATATCTACAATACTCTGAATGGGAGTGGGCGGATGACCCGGCTGGTCTACAATATTATTTGGAGCTGATGAATGATC
GATATCATTTACCACTAATGGTTGTTGAAAATGGATTGGGTGCCAAGGATTATTTGACAGTAGATGGTCAAATCCATGAT
GTATACCGGATTAGCTATCTACGTAAACATATTCAAGCAATGGCAACGGCAATTCACCATGGCGTCAAACTGATTGGTTA
TACCAGTTGGGGCTGTATTGATTTAGTTTCTGCAGGTACAGGGCAAATGTCTAAACGATACGGATTGATTTATGTGGATC
GTCAAGACGATGGTAGTGGAACTCTGGCCCGAATCCCGAAGGACTCTTTCTACTGGTATCAGCGAGTGATTGCATCCAAT
GGTCAAGATCTGGGTCCCTCGGCCTCAAATTAA

Upstream 100 bases:

>100_bases
CCAAAGAGGCGGCAGTTGAAGCTGAAGAAAAGCGGGCGGCAGTTTCGAATCAAGGTAATGTGCCCATGCAACCTGTGAAG
CCTGAGAAAGAGGTGTGAAC

Downstream 100 bases:

>100_bases
ATGAGTGTTGCTCAGCAGTTTTCGTCGATATCGGATGAAGACTGCTTTTGGTTGTTCAATAAGATAATTAGCCGCGTCAA
ACAAAACAAGAGGATACCAC

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MQFPADFYWGGATAANQCEGAYDVDGRGLTMKDITTMGGLNQRRQVTYLQADGTPGKGDSIPAGAHGAVLPDDYYPNQTS
IDFYHRYQEDIALFAEMGFKMYRMSISWSRIFPRGDENEPNQAGLDFYRRVFETLKKYEIEPLVTISHFDMPLYLEETYG
GWNDRRMIGFYQHYAETLFTAYRGLVKHWITFNEINNTIMFLALRAKAGDADYQRAYQQLHYQFVASALAVQQAHAIDGE
NKVGCMICGITSYPLTCDPADVLQNRYVWEQNIYYCGDVQCQGQYPTYARRLWNEHQVDLDITDSDLEALKAGTVDWYTF
SYYMSTAVTTHEVSATVGGNFSMGARNPYLQYSEWEWADDPAGLQYYLELMNDRYHLPLMVVENGLGAKDYLTVDGQIHD
VYRISYLRKHIQAMATAIHHGVKLIGYTSWGCIDLVSAGTGQMSKRYGLIYVDRQDDGSGTLARIPKDSFYWYQRVIASN
GQDLGPSASN

Sequences:

>Translated_490_residues
MQFPADFYWGGATAANQCEGAYDVDGRGLTMKDITTMGGLNQRRQVTYLQADGTPGKGDSIPAGAHGAVLPDDYYPNQTS
IDFYHRYQEDIALFAEMGFKMYRMSISWSRIFPRGDENEPNQAGLDFYRRVFETLKKYEIEPLVTISHFDMPLYLEETYG
GWNDRRMIGFYQHYAETLFTAYRGLVKHWITFNEINNTIMFLALRAKAGDADYQRAYQQLHYQFVASALAVQQAHAIDGE
NKVGCMICGITSYPLTCDPADVLQNRYVWEQNIYYCGDVQCQGQYPTYARRLWNEHQVDLDITDSDLEALKAGTVDWYTF
SYYMSTAVTTHEVSATVGGNFSMGARNPYLQYSEWEWADDPAGLQYYLELMNDRYHLPLMVVENGLGAKDYLTVDGQIHD
VYRISYLRKHIQAMATAIHHGVKLIGYTSWGCIDLVSAGTGQMSKRYGLIYVDRQDDGSGTLARIPKDSFYWYQRVIASN
GQDLGPSASN
>Mature_490_residues
MQFPADFYWGGATAANQCEGAYDVDGRGLTMKDITTMGGLNQRRQVTYLQADGTPGKGDSIPAGAHGAVLPDDYYPNQTS
IDFYHRYQEDIALFAEMGFKMYRMSISWSRIFPRGDENEPNQAGLDFYRRVFETLKKYEIEPLVTISHFDMPLYLEETYG
GWNDRRMIGFYQHYAETLFTAYRGLVKHWITFNEINNTIMFLALRAKAGDADYQRAYQQLHYQFVASALAVQQAHAIDGE
NKVGCMICGITSYPLTCDPADVLQNRYVWEQNIYYCGDVQCQGQYPTYARRLWNEHQVDLDITDSDLEALKAGTVDWYTF
SYYMSTAVTTHEVSATVGGNFSMGARNPYLQYSEWEWADDPAGLQYYLELMNDRYHLPLMVVENGLGAKDYLTVDGQIHD
VYRISYLRKHIQAMATAIHHGVKLIGYTSWGCIDLVSAGTGQMSKRYGLIYVDRQDDGSGTLARIPKDSFYWYQRVIASN
GQDLGPSASN

Specific function: Can hydrolyze salicin, cellobiose, and probably arbutin [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=517, Percent_Identity=27.6595744680851, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI32481206, Length=509, Percent_Identity=26.9155206286837, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI13273313, Length=509, Percent_Identity=26.3261296660118, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI24497614, Length=493, Percent_Identity=27.3833671399594, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI28376633, Length=499, Percent_Identity=23.4468937875752, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI1789070, Length=497, Percent_Identity=48.2897384305835, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI2367174, Length=500, Percent_Identity=44.8, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI2367270, Length=489, Percent_Identity=47.4437627811861, Blast_Score=432, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17552856, Length=510, Percent_Identity=28.2352941176471, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17539390, Length=506, Percent_Identity=27.2727272727273, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21356577, Length=514, Percent_Identity=25.4863813229572, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 55837; Mature: 55837

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFPADFYWGGATAANQCEGAYDVDGRGLTMKDITTMGGLNQRRQVTYLQADGTPGKGDS
CCCCCCCCCCCCCCHHCCCCCEECCCCCEEHHHHHHHCCCCCCCEEEEEEECCCCCCCCC
IPAGAHGAVLPDDYYPNQTSIDFYHRYQEDIALFAEMGFKMYRMSISWSRIFPRGDENEP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEHHCCCCCCCCCCC
NQAGLDFYRRVFETLKKYEIEPLVTISHFDMPLYLEETYGGWNDRRMIGFYQHYAETLFT
CHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCEEHHHHHHHHHHHHH
AYRGLVKHWITFNEINNTIMFLALRAKAGDADYQRAYQQLHYQFVASALAVQQAHAIDGE
HHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NKVGCMICGITSYPLTCDPADVLQNRYVWEQNIYYCGDVQCQGQYPTYARRLWNEHQVDL
CCCEEEEEECCCCCCCCCHHHHHHCCEEEECCCEEECCEEECCCCCHHHHHHCCCCEEEE
DITDSDLEALKAGTVDWYTFSYYMSTAVTTHEVSATVGGNFSMGARNPYLQYSEWEWADD
EECCHHHHHHHCCCCEEEEEEEHHHHHEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
PAGLQYYLELMNDRYHLPLMVVENGLGAKDYLTVDGQIHDVYRISYLRKHIQAMATAIHH
CHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHC
GVKLIGYTSWGCIDLVSAGTGQMSKRYGLIYVDRQDDGSGTLARIPKDSFYWYQRVIASN
CEEEEEECCCCHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEECCCHHHHHHHHHHHCC
GQDLGPSASN
CCCCCCCCCC
>Mature Secondary Structure
MQFPADFYWGGATAANQCEGAYDVDGRGLTMKDITTMGGLNQRRQVTYLQADGTPGKGDS
CCCCCCCCCCCCCCHHCCCCCEECCCCCEEHHHHHHHCCCCCCCEEEEEEECCCCCCCCC
IPAGAHGAVLPDDYYPNQTSIDFYHRYQEDIALFAEMGFKMYRMSISWSRIFPRGDENEP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEHHCCCCCCCCCCC
NQAGLDFYRRVFETLKKYEIEPLVTISHFDMPLYLEETYGGWNDRRMIGFYQHYAETLFT
CHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCEEHHHHHHHHHHHHH
AYRGLVKHWITFNEINNTIMFLALRAKAGDADYQRAYQQLHYQFVASALAVQQAHAIDGE
HHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NKVGCMICGITSYPLTCDPADVLQNRYVWEQNIYYCGDVQCQGQYPTYARRLWNEHQVDL
CCCEEEEEECCCCCCCCCHHHHHHCCEEEECCCEEECCEEECCCCCHHHHHHCCCCEEEE
DITDSDLEALKAGTVDWYTFSYYMSTAVTTHEVSATVGGNFSMGARNPYLQYSEWEWADD
EECCHHHHHHHCCCCEEEEEEEHHHHHEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
PAGLQYYLELMNDRYHLPLMVVENGLGAKDYLTVDGQIHDVYRISYLRKHIQAMATAIHH
CHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHC
GVKLIGYTSWGCIDLVSAGTGQMSKRYGLIYVDRQDDGSGTLARIPKDSFYWYQRVIASN
CEEEEEECCCCHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEECCCHHHHHHHHHHHCC
GQDLGPSASN
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]