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The map label for this gene is pts15C [H]

Identifier: 28378132

GI number: 28378132

Start: 1282987

End: 1284342

Strand: Direct

Name: pts15C [H]

Synonym: lp_1400

Alternate gene names: 28378132

Gene position: 1282987-1284342 (Clockwise)

Preceding gene: 28378131

Following gene: 28378133

Centisome position: 38.78

GC content: 42.99

Gene sequence:

>1356_bases
ATGGAACGCTTTTTTAATAGTAAACTGATGGTCGGCTTACAACGCGCCGGCCAGGGACTTGGCAATAATAAGTTTATTAC
CGCATTACAGGCAGCTATGATGTCTTTGATGGGTATTCTAATGGTTGGCGCGATCTTTCAAATCACGATGTCAGTACTTG
GACCGACGATTTTTGGCGTGTTATCAGCAAAGAGTATTCTGTTAGCCTACTTGAAGATTCCATTCCAATTCTCAATGAAT
TCTTTATCAATTTGGGTTGTTTTATTCTTGGGATTCCATTACGCCAAGAATTTAAAAATGACGGCCCCAATCATGAATGC
GATTGACGCATTATTTGGTTTTTGGATTGTGGCCGGTGGGTTGATTGTCAGTAAGACTGGAACAGTTAGCATTGATACAA
CCTATTTAGGTGCTCAGGGAATGTTTATTGGCTTCGTCGTAGTTTGGATTTCCGTCCGCGTTGAGAAATTATGTACAGAC
AAGAATATTCGAATCAACATGCCAGATGCAGTCCCCCAATTTCTCCAAGACGGTTTTGCATCCATTGTGCCATTATTGTT
TAACGCCGTGATTTTCTTGGGCGCATCGTTATTAGTCACGGTTGGAACAGCCGGTAAGTATAATATTTGTTCGGGTTTTA
TGGAATTACTGGCAATTCCACTGAGTGCACTGACTTCAACCATAGGTATTTTTGTCCTGTGTATCTTGGGTGCTTTATTA
TGGACCTTTGGCATTCATGGTACGATGATTTTAGTCCCAATTGTGATGCCGTTAGTGATTCAAGCGGTTTCTGAAAATGC
CGCATTGCAGGCAGCTGGGAAGCCGGTGGTGTTTGCACCTGTGATGTTGTTCTCATTTATGACCGTAGCAGGTGGAACTG
GGAATACCCTGCCGTTAGCGCTAATGGCGATGCGTTCTAAATCTAAACAGCTGAGTGCCGTTGGTAAGCTGTCAATTATC
CCCGGCTGGTTCAAAATTAATGAACCCATGACTTTTGGTATTCCAATTATGTATAATCCGATTATGAGTATCCCGTTTGT
CCTGAATATTCCGGTAGTTATGTTGTGTGCACTCGTACTATACAAGATTGGATTTTTAACACCGGGGTGGATTGTAATTA
CCGCGATTTTACCCATGGGTTTTGGAAGTTATCTCAGTACGCTCAGTTGGCGCAACGCATTGACTGACTATTTGATGCTG
ATTCCGTCAGCGCTTATCTGGTATCCCTTCTTTAAAGTTTATGAAAAGCAACTGATTGCCAAAGAGGCGGCAGTTGAAGC
TGAAGAAAAGCGGGCGGCAGTTTCGAATCAAGGTAATGTGCCCATGCAACCTGTGAAGCCTGAGAAAGAGGTGTGA

Upstream 100 bases:

>100_bases
TCCACCATTGGACTATGCGGTGGGTGACAGTGCCCATATTTTCAAACTGGCCGAGAACTTGGTGTGATGACGAGGGATTA
GTATTCGGGGGTGAAGGACG

Downstream 100 bases:

>100_bases
ACATGCAATTTCCGGCTGATTTTTACTGGGGTGGGGCTACTGCCGCTAATCAATGCGAAGGTGCTTATGATGTTGATGGT
CGGGGCCTGACTATGAAGGA

Product: beta-glucosides PTS, EIIC

Products: protein histidine; sugar phosphate

Alternate protein names: Glucomannan utilization protein C; PTS system oligo-beta-mannoside-specific EIIC component [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MERFFNSKLMVGLQRAGQGLGNNKFITALQAAMMSLMGILMVGAIFQITMSVLGPTIFGVLSAKSILLAYLKIPFQFSMN
SLSIWVVLFLGFHYAKNLKMTAPIMNAIDALFGFWIVAGGLIVSKTGTVSIDTTYLGAQGMFIGFVVVWISVRVEKLCTD
KNIRINMPDAVPQFLQDGFASIVPLLFNAVIFLGASLLVTVGTAGKYNICSGFMELLAIPLSALTSTIGIFVLCILGALL
WTFGIHGTMILVPIVMPLVIQAVSENAALQAAGKPVVFAPVMLFSFMTVAGGTGNTLPLALMAMRSKSKQLSAVGKLSII
PGWFKINEPMTFGIPIMYNPIMSIPFVLNIPVVMLCALVLYKIGFLTPGWIVITAILPMGFGSYLSTLSWRNALTDYLML
IPSALIWYPFFKVYEKQLIAKEAAVEAEEKRAAVSNQGNVPMQPVKPEKEV

Sequences:

>Translated_451_residues
MERFFNSKLMVGLQRAGQGLGNNKFITALQAAMMSLMGILMVGAIFQITMSVLGPTIFGVLSAKSILLAYLKIPFQFSMN
SLSIWVVLFLGFHYAKNLKMTAPIMNAIDALFGFWIVAGGLIVSKTGTVSIDTTYLGAQGMFIGFVVVWISVRVEKLCTD
KNIRINMPDAVPQFLQDGFASIVPLLFNAVIFLGASLLVTVGTAGKYNICSGFMELLAIPLSALTSTIGIFVLCILGALL
WTFGIHGTMILVPIVMPLVIQAVSENAALQAAGKPVVFAPVMLFSFMTVAGGTGNTLPLALMAMRSKSKQLSAVGKLSII
PGWFKINEPMTFGIPIMYNPIMSIPFVLNIPVVMLCALVLYKIGFLTPGWIVITAILPMGFGSYLSTLSWRNALTDYLML
IPSALIWYPFFKVYEKQLIAKEAAVEAEEKRAAVSNQGNVPMQPVKPEKEV
>Mature_451_residues
MERFFNSKLMVGLQRAGQGLGNNKFITALQAAMMSLMGILMVGAIFQITMSVLGPTIFGVLSAKSILLAYLKIPFQFSMN
SLSIWVVLFLGFHYAKNLKMTAPIMNAIDALFGFWIVAGGLIVSKTGTVSIDTTYLGAQGMFIGFVVVWISVRVEKLCTD
KNIRINMPDAVPQFLQDGFASIVPLLFNAVIFLGASLLVTVGTAGKYNICSGFMELLAIPLSALTSTIGIFVLCILGALL
WTFGIHGTMILVPIVMPLVIQAVSENAALQAAGKPVVFAPVMLFSFMTVAGGTGNTLPLALMAMRSKSKQLSAVGKLSII
PGWFKINEPMTFGIPIMYNPIMSIPFVLNIPVVMLCALVLYKIGFLTPGWIVITAILPMGFGSYLSTLSWRNALTDYLML
IPSALIWYPFFKVYEKQLIAKEAAVEAEEKRAAVSNQGNVPMQPVKPEKEV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=321, Percent_Identity=30.5295950155763, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: 2.7.1.69

Molecular weight: Translated: 48926; Mature: 48926

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFFNSKLMVGLQRAGQGLGNNKFITALQAAMMSLMGILMVGAIFQITMSVLGPTIFGV
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSAKSILLAYLKIPFQFSMNSLSIWVVLFLGFHYAKNLKMTAPIMNAIDALFGFWIVAGG
HHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
LIVSKTGTVSIDTTYLGAQGMFIGFVVVWISVRVEKLCTDKNIRINMPDAVPQFLQDGFA
EEEECCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHH
SIVPLLFNAVIFLGASLLVTVGTAGKYNICSGFMELLAIPLSALTSTIGIFVLCILGALL
HHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WTFGIHGTMILVPIVMPLVIQAVSENAALQAAGKPVVFAPVMLFSFMTVAGGTGNTLPLA
HHHCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
LMAMRSKSKQLSAVGKLSIIPGWFKINEPMTFGIPIMYNPIMSIPFVLNIPVVMLCALVL
HHHHHHHHHHHHHHCCCEECCCEEEECCCCEECCCHHHCHHHHCCHHHCCHHHHHHHHHH
YKIGFLTPGWIVITAILPMGFGSYLSTLSWRNALTDYLMLIPSALIWYPFFKVYEKQLIA
HHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEAAVEAEEKRAAVSNQGNVPMQPVKPEKEV
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MERFFNSKLMVGLQRAGQGLGNNKFITALQAAMMSLMGILMVGAIFQITMSVLGPTIFGV
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSAKSILLAYLKIPFQFSMNSLSIWVVLFLGFHYAKNLKMTAPIMNAIDALFGFWIVAGG
HHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
LIVSKTGTVSIDTTYLGAQGMFIGFVVVWISVRVEKLCTDKNIRINMPDAVPQFLQDGFA
EEEECCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHH
SIVPLLFNAVIFLGASLLVTVGTAGKYNICSGFMELLAIPLSALTSTIGIFVLCILGALL
HHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WTFGIHGTMILVPIVMPLVIQAVSENAALQAAGKPVVFAPVMLFSFMTVAGGTGNTLPLA
HHHCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
LMAMRSKSKQLSAVGKLSIIPGWFKINEPMTFGIPIMYNPIMSIPFVLNIPVVMLCALVL
HHHHHHHHHHHHHHCCCEECCCEEEECCCCEECCCHHHCHHHHCCHHHCCHHHHHHHHHH
YKIGFLTPGWIVITAILPMGFGSYLSTLSWRNALTDYLMLIPSALIWYPFFKVYEKQLIA
HHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEAAVEAEEKRAAVSNQGNVPMQPVKPEKEV
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]