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The map label for this gene is als [H]

Identifier: 28377817

GI number: 28377817

Start: 927175

End: 928857

Strand: Direct

Name: als [H]

Synonym: lp_1005

Alternate gene names: 28377817

Gene position: 927175-928857 (Clockwise)

Preceding gene: 28377816

Following gene: 28377818

Centisome position: 28.03

GC content: 48.6

Gene sequence:

>1683_bases
ATGCCAGATAAGAAATATTACGGGGCCGATGCGATCGTCGACAGCCTTGTAAACCACGACGTTAAGTACGTATTCGGGAT
TCCAGGCGCTAAGATCGATCGGGTCTTTGAGCGCTTGGAGCACCCAGTCAACCCGAAAAGTCCGCGGTTGATCGTGACGC
GGCATGAACAGAATGCCGCGTTTATCGCTGCCGGAATCGGTCGGATTACTGGGAAACCTGGTGTAGTGATGACAACATCT
GGCCCCGGTGCCAGCAATTTAGCCACGGGGCTCGTAACGGCAACTGCTGAAGGTGACCCAGTCTTGGCAATTTCTGGTCA
AGTTCAGCGTGCTGACTTGTTACGGTTGACCCACCAAAGTATGAATAACGCGGCACTATTTAAGCCGATTACAAAGTACA
GTGCGGAAGTTCAAGAACCGGAAAACATTTCGGAAGTGCTCGCAAACGCCTACCAAGAAGCCACAGCTGCTAAGCAGGGT
GCCAGCTTTGTTAGTGTGCCACAAGATGTCACTGATTCGATTGTACGCACACCTGTTATTACACCAATCCAAGCACCTAA
GTTAGGGCCTGCTAGCCCGGTCGAAGCCACGTTATTAGCACAAAAAATCAAAGCAGCTAAGTTGCCGGTCTTGCTAGTTG
GGATGCGGGCTTCTTCACCAGAAGTGACGAAAGCAATTCGGAACTTGGTGGCTGCTGCTAATCTACCAGTTGTCGAGACT
TTCCAAGCGGCCGGCGTGATTTCACGTGATTTGGAAGCCAACCATTTCTTCGGTCGGGTCGGGTTATTCCGTAACCAACC
GGGGGACATGTTACTAAAGAAGTCCGACTTAGTGATTGCGGTCGGTTATGACCCGATTGAATATGAACCCCGCAACTGGA
ATGCGGAAGGCAAATCCCGTATCGTGGTTATCGATGCAATGCGCGCTGAAATTGATCATAATTTTCAACCGGAAACCGAA
TTGATCGGTGATATTGCCCAGACGTTAGACTTTTTGTTACCGTACATGAAGGGCTATGATATTAGCGATAGTGCGCGAGC
TTACTTGGGTGAATTACAAGAACGGCTTCAGACGCGTGATTTCGTACCTAATATTGATAAGCAGTCCAAGTTAAATCATC
CGCTGTCAGTAATTGCAGCCTTACAACAACGGGTCAGTGATGACATGACAGTGACGGTCGATGTCGGCAGTCATTATATC
TGGATGGCGCGTCACTTTCGGAGCTATGAACCACGGCACTTGTTGTTCAGTAATGGGATGCAAACGCTAGGGGTGGCCTT
ACCTTGGGCAATCGCAGCGGCTTTGGTTCGTCCAGATACGCAAATCGTGTCTGTCTCTGGTGATGGTGGTTTCCTATTTT
CCGCCCAAGAGCTGGAAACGGCTGTTCGACTGAAGCAAAATATCGTTCATTTGATCTGGAATGATGGCACCTACGATATG
GTTAAATTCCAAGAAGAAATGAAGTATGGCGAGGATGCAGCGGTCCACTTTGGCCCAGTTGATTTTGTGAAGTATGCTGA
AAGCTTTGGTGCGACTGGCTTGCGGGTAAACCAACCTGCTGATTTGGAAAAAGTGCTTGATCAGGCCTTTGCCACGGATG
GTCCAGTTGTGGTGGATATTCCAATTGATTATTCTGATAACAAAGCGTTAGGCAAGACAATGTTACCAGACCAGTTTTAT
TAA

Upstream 100 bases:

>100_bases
TCAAAATGATACACTTTATTTCACAAGGCTGAAAATGCTGTTATTAAAGCGTTTTTTATGGTATTCTAACAATGAATTTC
ACAAGATAGAGGGGAACAAC

Downstream 100 bases:

>100_bases
AATTAACATTAGTGACCGGTTGTCAAAAGATGGTCGGTCAGATATAATAGTTGAGTATATCGAAAGAAGAGGATGCGGTT
AACAATAGTAGCCGGCTGGA

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MPDKKYYGADAIVDSLVNHDVKYVFGIPGAKIDRVFERLEHPVNPKSPRLIVTRHEQNAAFIAAGIGRITGKPGVVMTTS
GPGASNLATGLVTATAEGDPVLAISGQVQRADLLRLTHQSMNNAALFKPITKYSAEVQEPENISEVLANAYQEATAAKQG
ASFVSVPQDVTDSIVRTPVITPIQAPKLGPASPVEATLLAQKIKAAKLPVLLVGMRASSPEVTKAIRNLVAAANLPVVET
FQAAGVISRDLEANHFFGRVGLFRNQPGDMLLKKSDLVIAVGYDPIEYEPRNWNAEGKSRIVVIDAMRAEIDHNFQPETE
LIGDIAQTLDFLLPYMKGYDISDSARAYLGELQERLQTRDFVPNIDKQSKLNHPLSVIAALQQRVSDDMTVTVDVGSHYI
WMARHFRSYEPRHLLFSNGMQTLGVALPWAIAAALVRPDTQIVSVSGDGGFLFSAQELETAVRLKQNIVHLIWNDGTYDM
VKFQEEMKYGEDAAVHFGPVDFVKYAESFGATGLRVNQPADLEKVLDQAFATDGPVVVDIPIDYSDNKALGKTMLPDQFY

Sequences:

>Translated_560_residues
MPDKKYYGADAIVDSLVNHDVKYVFGIPGAKIDRVFERLEHPVNPKSPRLIVTRHEQNAAFIAAGIGRITGKPGVVMTTS
GPGASNLATGLVTATAEGDPVLAISGQVQRADLLRLTHQSMNNAALFKPITKYSAEVQEPENISEVLANAYQEATAAKQG
ASFVSVPQDVTDSIVRTPVITPIQAPKLGPASPVEATLLAQKIKAAKLPVLLVGMRASSPEVTKAIRNLVAAANLPVVET
FQAAGVISRDLEANHFFGRVGLFRNQPGDMLLKKSDLVIAVGYDPIEYEPRNWNAEGKSRIVVIDAMRAEIDHNFQPETE
LIGDIAQTLDFLLPYMKGYDISDSARAYLGELQERLQTRDFVPNIDKQSKLNHPLSVIAALQQRVSDDMTVTVDVGSHYI
WMARHFRSYEPRHLLFSNGMQTLGVALPWAIAAALVRPDTQIVSVSGDGGFLFSAQELETAVRLKQNIVHLIWNDGTYDM
VKFQEEMKYGEDAAVHFGPVDFVKYAESFGATGLRVNQPADLEKVLDQAFATDGPVVVDIPIDYSDNKALGKTMLPDQFY
>Mature_559_residues
PDKKYYGADAIVDSLVNHDVKYVFGIPGAKIDRVFERLEHPVNPKSPRLIVTRHEQNAAFIAAGIGRITGKPGVVMTTSG
PGASNLATGLVTATAEGDPVLAISGQVQRADLLRLTHQSMNNAALFKPITKYSAEVQEPENISEVLANAYQEATAAKQGA
SFVSVPQDVTDSIVRTPVITPIQAPKLGPASPVEATLLAQKIKAAKLPVLLVGMRASSPEVTKAIRNLVAAANLPVVETF
QAAGVISRDLEANHFFGRVGLFRNQPGDMLLKKSDLVIAVGYDPIEYEPRNWNAEGKSRIVVIDAMRAEIDHNFQPETEL
IGDIAQTLDFLLPYMKGYDISDSARAYLGELQERLQTRDFVPNIDKQSKLNHPLSVIAALQQRVSDDMTVTVDVGSHYIW
MARHFRSYEPRHLLFSNGMQTLGVALPWAIAAALVRPDTQIVSVSGDGGFLFSAQELETAVRLKQNIVHLIWNDGTYDMV
KFQEEMKYGEDAAVHFGPVDFVKYAESFGATGLRVNQPADLEKVLDQAFATDGPVVVDIPIDYSDNKALGKTMLPDQFY

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=583, Percent_Identity=26.7581475128645, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI21361361, Length=592, Percent_Identity=25.1689189189189, Blast_Score=146, Evalue=5e-35,
Organism=Escherichia coli, GI1790104, Length=555, Percent_Identity=30.0900900900901, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI87081685, Length=562, Percent_Identity=29.1814946619217, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1786717, Length=582, Percent_Identity=24.5704467353952, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1787096, Length=549, Percent_Identity=26.0473588342441, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1788716, Length=566, Percent_Identity=24.9116607773852, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17542570, Length=578, Percent_Identity=24.2214532871972, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17531299, Length=559, Percent_Identity=25.2236135957066, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17531301, Length=559, Percent_Identity=25.2236135957066, Blast_Score=123, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=564, Percent_Identity=27.3049645390071, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320816, Length=544, Percent_Identity=26.2867647058824, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19922626, Length=570, Percent_Identity=24.3859649122807, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61233; Mature: 61101

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDKKYYGADAIVDSLVNHDVKYVFGIPGAKIDRVFERLEHPVNPKSPRLIVTRHEQNAA
CCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCE
FIAAGIGRITGKPGVVMTTSGPGASNLATGLVTATAEGDPVLAISGQVQRADLLRLTHQS
EEEECHHHCCCCCCEEEEECCCCCCHHHHCEEEEECCCCEEEEECCCCHHHHHHHHHHHC
MNNAALFKPITKYSAEVQEPENISEVLANAYQEATAAKQGASFVSVPQDVTDSIVRTPVI
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHCCCC
TPIQAPKLGPASPVEATLLAQKIKAAKLPVLLVGMRASSPEVTKAIRNLVAAANLPVVET
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHH
FQAAGVISRDLEANHFFGRVGLFRNQPGDMLLKKSDLVIAVGYDPIEYEPRNWNAEGKSR
HHHHCHHHHCCCCCHHHEECCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCE
IVVIDAMRAEIDHNFQPETELIGDIAQTLDFLLPYMKGYDISDSARAYLGELQERLQTRD
EEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
FVPNIDKQSKLNHPLSVIAALQQRVSDDMTVTVDVGSHYIWMARHFRSYEPRHLLFSNGM
CCCCCCCHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEEHHHHCCCCCCCHHHHCCH
QTLGVALPWAIAAALVRPDTQIVSVSGDGGFLFSAQELETAVRLKQNIVHLIWNDGTYDM
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHHHHCEEEEEECCCCCHH
VKFQEEMKYGEDAAVHFGPVDFVKYAESFGATGLRVNQPADLEKVLDQAFATDGPVVVDI
HHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCCCEEEEE
PIDYSDNKALGKTMLPDQFY
ECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PDKKYYGADAIVDSLVNHDVKYVFGIPGAKIDRVFERLEHPVNPKSPRLIVTRHEQNAA
CCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCE
FIAAGIGRITGKPGVVMTTSGPGASNLATGLVTATAEGDPVLAISGQVQRADLLRLTHQS
EEEECHHHCCCCCCEEEEECCCCCCHHHHCEEEEECCCCEEEEECCCCHHHHHHHHHHHC
MNNAALFKPITKYSAEVQEPENISEVLANAYQEATAAKQGASFVSVPQDVTDSIVRTPVI
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHCCCC
TPIQAPKLGPASPVEATLLAQKIKAAKLPVLLVGMRASSPEVTKAIRNLVAAANLPVVET
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHH
FQAAGVISRDLEANHFFGRVGLFRNQPGDMLLKKSDLVIAVGYDPIEYEPRNWNAEGKSR
HHHHCHHHHCCCCCHHHEECCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCE
IVVIDAMRAEIDHNFQPETELIGDIAQTLDFLLPYMKGYDISDSARAYLGELQERLQTRD
EEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
FVPNIDKQSKLNHPLSVIAALQQRVSDDMTVTVDVGSHYIWMARHFRSYEPRHLLFSNGM
CCCCCCCHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEEHHHHCCCCCCCHHHHCCH
QTLGVALPWAIAAALVRPDTQIVSVSGDGGFLFSAQELETAVRLKQNIVHLIWNDGTYDM
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHHHHCEEEEEECCCCCHH
VKFQEEMKYGEDAAVHFGPVDFVKYAESFGATGLRVNQPADLEKVLDQAFATDGPVVVDI
HHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCCCEEEEE
PIDYSDNKALGKTMLPDQFY
ECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]