Click here to switch to the map view.

The map label for this gene is hsdM [H]

Identifier: 28377765

GI number: 28377765

Start: 870888

End: 872474

Strand: Direct

Name: hsdM [H]

Synonym: lp_0939

Alternate gene names: 28377765

Gene position: 870888-872474 (Clockwise)

Preceding gene: 28377764

Following gene: 28377766

Centisome position: 26.32

GC content: 41.4

Gene sequence:

>1587_bases
ATGAGTCATATTGAACAGGCCGAACAACAGGCTGAACTACAAAAAAGATTATGGGCTGTGGCAAATGATCTGCGCGGTAA
CATGGATGCCAGTGAGTATCGAAACTATATCTTAGGATTGATCTTTTATCGTTTTTTGTCCGAAAAAGTTGAAAACTATG
CAAATGAGTTACTTCAAGACGATGATGTTGACTTTGCAGATGCGGAACAAGATGCCGATTTGATGCAAGATTTGAAGGAT
GAAGTGATTGACGTCTTAGGGTTCTTCATTGAACCACGATATCTATTTACGACTATGGTCAAGAAGATTAACGCTGGTGA
TTTTGACGTTGAAATGTTACAAAATGCCATCAACGAAGTCCAGAATTCGACCTTAGGCAAAGAGTCAGAAAATGACTTTA
AAGGGCTATTCGAAGACTTGGATTTGCAGTCATCACGGTTAGGAAATACAGTTGCCAAGCGTAGTGAACTGATTGCTAAA
GTGATTTTATCCTTGTCGAATATTGATTTTGGTGAACAAGATATTAAAATCGATATTTTGGGTGATGCCTATGAATATTT
GATTGGTCAATTTGCCGCTTCGGCCGGGAAAAAGGCGGGCGAATTTTATACGCCACAACAGGTTTCTAAGTTATTGGCAA
GAATTGTTACGGCCGGTAAAGATCGTTTGAAGACGGTTTATGATCCTACGATGGGCTCCGGGTCGCTGTTATTACAATTG
GGTAATTACGCAACGATTGGAAACTACTATGGTCAAGAAATCAACGGTACGACTTACAACTTGGCGCGAATGAACTTGTT
GATGCATGAGGTTAGTTATAATCGGTTTGATTTACGTCAAGGCGATACTTTAGAGGAAGATCACTTTGATGATCTAACTT
TTGACGCTGTCGTTGCCAATCCACCTTATTCGGCTAAGTGGAATCCAGATGATAAATTAGATGATGAACGTTTTCGGAAA
TATGGGAAGACGGCACCGAAGTCGAAGGCGGATTTTGCGTTTGTTGAACACATGCTCTATCACTTAAATAATGAAGGCAC
GATGGCAGTTGTATTACCACATGGTGTCTTATTCCGCGGTGCTGCGGAAGGAAAAATCCGACAGTACATGATTGAACATG
ACAACGTCTTAGATGCCGTCATCGGATTACCAGCGAACTTATTCTACGGAACTAGTATTCCGACCGTTGTATTGGTCTTC
AAGAAGGGGCGCGAACGTCAGGATATCTTCTTTATTGACGCGTCTAATGATTTTGAAAAGGGTAAGAACCAAAACAACTT
AACGGACGAAAATGTCGATAAGATTTTGGAAACCCTTGAAAAACGTGAAGATGTGGATAAGTATGCTCATCGTGCAGAAG
TAGCTGAGATTGTCGAAAACGAGTATAACTTAAACATTCCGCGATACGTCGATACATTCGAAGAGGAACCACCTGTTGAT
GTGGATAAGTTGGTTAAGGAAATGGGCGAAGCGGATGCCAAAATCAACGACCTACAATCTGAATTATCCGGAATGATGGC
CGATTTAGTGGCGACTGACCCTGTCGCTCAACAGCAGCTTGAGGCCATTAAGAGGATGTTACAATGA

Upstream 100 bases:

>100_bases
GGGCTCGGCTTTCGAGAGAAACGGCAGGCCAAACGCGAGATTAAGGATTTTGTATCGACTATCGACCGAAAGTATTCACT
AAGCTAGGAGAATGAATTTA

Downstream 100 bases:

>100_bases
AAAACGAAAATTTAGTGCCAAAAGTGCGGTTTAAGGGGTTTAGTGACCCTTGGGAGCAGCGTAAGCTTGGAGAACTTGGC
AAAATTCAAGGCGGTGGAAC

Product: site-specific DNA-methyltransferase (adenine-specific), HsdM subunit

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSHIEQAEQQAELQKRLWAVANDLRGNMDASEYRNYILGLIFYRFLSEKVENYANELLQDDDVDFADAEQDADLMQDLKD
EVIDVLGFFIEPRYLFTTMVKKINAGDFDVEMLQNAINEVQNSTLGKESENDFKGLFEDLDLQSSRLGNTVAKRSELIAK
VILSLSNIDFGEQDIKIDILGDAYEYLIGQFAASAGKKAGEFYTPQQVSKLLARIVTAGKDRLKTVYDPTMGSGSLLLQL
GNYATIGNYYGQEINGTTYNLARMNLLMHEVSYNRFDLRQGDTLEEDHFDDLTFDAVVANPPYSAKWNPDDKLDDERFRK
YGKTAPKSKADFAFVEHMLYHLNNEGTMAVVLPHGVLFRGAAEGKIRQYMIEHDNVLDAVIGLPANLFYGTSIPTVVLVF
KKGRERQDIFFIDASNDFEKGKNQNNLTDENVDKILETLEKREDVDKYAHRAEVAEIVENEYNLNIPRYVDTFEEEPPVD
VDKLVKEMGEADAKINDLQSELSGMMADLVATDPVAQQQLEAIKRMLQ

Sequences:

>Translated_528_residues
MSHIEQAEQQAELQKRLWAVANDLRGNMDASEYRNYILGLIFYRFLSEKVENYANELLQDDDVDFADAEQDADLMQDLKD
EVIDVLGFFIEPRYLFTTMVKKINAGDFDVEMLQNAINEVQNSTLGKESENDFKGLFEDLDLQSSRLGNTVAKRSELIAK
VILSLSNIDFGEQDIKIDILGDAYEYLIGQFAASAGKKAGEFYTPQQVSKLLARIVTAGKDRLKTVYDPTMGSGSLLLQL
GNYATIGNYYGQEINGTTYNLARMNLLMHEVSYNRFDLRQGDTLEEDHFDDLTFDAVVANPPYSAKWNPDDKLDDERFRK
YGKTAPKSKADFAFVEHMLYHLNNEGTMAVVLPHGVLFRGAAEGKIRQYMIEHDNVLDAVIGLPANLFYGTSIPTVVLVF
KKGRERQDIFFIDASNDFEKGKNQNNLTDENVDKILETLEKREDVDKYAHRAEVAEIVENEYNLNIPRYVDTFEEEPPVD
VDKLVKEMGEADAKINDLQSELSGMMADLVATDPVAQQQLEAIKRMLQ
>Mature_527_residues
SHIEQAEQQAELQKRLWAVANDLRGNMDASEYRNYILGLIFYRFLSEKVENYANELLQDDDVDFADAEQDADLMQDLKDE
VIDVLGFFIEPRYLFTTMVKKINAGDFDVEMLQNAINEVQNSTLGKESENDFKGLFEDLDLQSSRLGNTVAKRSELIAKV
ILSLSNIDFGEQDIKIDILGDAYEYLIGQFAASAGKKAGEFYTPQQVSKLLARIVTAGKDRLKTVYDPTMGSGSLLLQLG
NYATIGNYYGQEINGTTYNLARMNLLMHEVSYNRFDLRQGDTLEEDHFDDLTFDAVVANPPYSAKWNPDDKLDDERFRKY
GKTAPKSKADFAFVEHMLYHLNNEGTMAVVLPHGVLFRGAAEGKIRQYMIEHDNVLDAVIGLPANLFYGTSIPTVVLVFK
KGRERQDIFFIDASNDFEKGKNQNNLTDENVDKILETLEKREDVDKYAHRAEVAEIVENEYNLNIPRYVDTFEEEPPVDV
DKLVKEMGEADAKINDLQSELSGMMADLVATDPVAQQQLEAIKRMLQ

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=363, Percent_Identity=22.8650137741047, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 59923; Mature: 59792

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHIEQAEQQAELQKRLWAVANDLRGNMDASEYRNYILGLIFYRFLSEKVENYANELLQD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DDVDFADAEQDADLMQDLKDEVIDVLGFFIEPRYLFTTMVKKINAGDFDVEMLQNAINEV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
QNSTLGKESENDFKGLFEDLDLQSSRLGNTVAKRSELIAKVILSLSNIDFGEQDIKIDIL
HHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
GDAYEYLIGQFAASAGKKAGEFYTPQQVSKLLARIVTAGKDRLKTVYDPTMGSGSLLLQL
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEE
GNYATIGNYYGQEINGTTYNLARMNLLMHEVSYNRFDLRQGDTLEEDHFDDLTFDAVVAN
CCEEEECHHCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCEEEEECC
PPYSAKWNPDDKLDDERFRKYGKTAPKSKADFAFVEHMLYHLNNEGTMAVVLPHGVLFRG
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEECC
AAEGKIRQYMIEHDNVLDAVIGLPANLFYGTSIPTVVLVFKKGRERQDIFFIDASNDFEK
CCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCHHC
GKNQNNLTDENVDKILETLEKREDVDKYAHRAEVAEIVENEYNLNIPRYVDTFEEEPPVD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCC
VDKLVKEMGEADAKINDLQSELSGMMADLVATDPVAQQQLEAIKRMLQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SHIEQAEQQAELQKRLWAVANDLRGNMDASEYRNYILGLIFYRFLSEKVENYANELLQD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DDVDFADAEQDADLMQDLKDEVIDVLGFFIEPRYLFTTMVKKINAGDFDVEMLQNAINEV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
QNSTLGKESENDFKGLFEDLDLQSSRLGNTVAKRSELIAKVILSLSNIDFGEQDIKIDIL
HHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
GDAYEYLIGQFAASAGKKAGEFYTPQQVSKLLARIVTAGKDRLKTVYDPTMGSGSLLLQL
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEE
GNYATIGNYYGQEINGTTYNLARMNLLMHEVSYNRFDLRQGDTLEEDHFDDLTFDAVVAN
CCEEEECHHCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCEEEEECC
PPYSAKWNPDDKLDDERFRKYGKTAPKSKADFAFVEHMLYHLNNEGTMAVVLPHGVLFRG
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEECC
AAEGKIRQYMIEHDNVLDAVIGLPANLFYGTSIPTVVLVFKKGRERQDIFFIDASNDFEK
CCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCHHC
GKNQNNLTDENVDKILETLEKREDVDKYAHRAEVAEIVENEYNLNIPRYVDTFEEEPPVD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCC
VDKLVKEMGEADAKINDLQSELSGMMADLVATDPVAQQQLEAIKRMLQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]