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The map label for this gene is pts12BCA [H]

Identifier: 28377737

GI number: 28377737

Start: 833821

End: 835683

Strand: Direct

Name: pts12BCA [H]

Synonym: lp_0905

Alternate gene names: 28377737

Gene position: 833821-835683 (Clockwise)

Preceding gene: 28377736

Following gene: 28377738

Centisome position: 25.2

GC content: 39.88

Gene sequence:

>1863_bases
ATGACATATAAAGAAATGAACGACCAGATTATTAATGGTGTCGGGGGTAAAAGTAATATTGAGTCAGTTGTACACTGTGC
AACAAGGCTGAGATTCGTGCTGAAGGATGAGGCCAAGGCAGATGATGATTTGGTACGGTCGATTCCAGGAATTTTACAAG
TTGTCAAAAAAGCGGGACAGTACCAATTAGTTATCGGAAATAATGTTGAGGATGTTTATAATGAACTTTCTGATATGTTA
CAGCTGGATGAAAATGTAGATCATAATCAGTCTAAGGATAAGCGGAGTATTTTCGACAAAATAATTAGTACGATTACTGG
CTCAATTGCACCTGCGATTCCATTGCTTGCTGGTGCTGGGATGGGGAAAGTATTGTTGCTCATTCTGACACTGTCTGGAT
TATTAACTGAAAAAAGTCAAACTTATCAGATGTTAAATTTGATTTTTGATACTGGCTATTTCTTTATGCCAGCATTTATT
GGATTTTCCGCTGCTAAGATTTTTAAGACAAACCAATTTCTAGGGGCATTTATGGGATTGGTCACTGTTAATCCAAATTG
GGTTGCAATGGTAGCGGCTGGGAAGCCTGTTAGTTTCATAGGTGTTCCTATCCAATTGGTTTCTTATTCTTCAACCTTGA
TTACAGCTATTTTATCCGTATGGTTAATGTCTTATATTGAAAAGTTTGTAAAGAAGATTACGCCAGGCATGATTAAAGTC
TTTGCAGAGCCGATGCTTATCATGTTGATTACTGCACCACTGACTTTTATTGTTCTTGGACCAATTGCCAATTTGATTTC
AATGGGAATTGCAAGTATAAGCATGTTCTTGTATGATAACGCCGGCTTTATCGCAATTCCGCTACTTGCTGCCGCATATC
CATGGTTAGTTTCTATTGGTATTCATAAAGCGCTCAGTCCAATTAGTATCCAATTAGTTGCGACACAGGGATTTGATCCA
ATAATTCGGGTTGTGGCTTTGTGTTCGAACATGTCACAAGCTGCCGCATCATTAGCAGTCGGTTTGAAAACCAAAAATAA
GCAGTTGAGAGCCTTAGCACTTTCATCCACTGTGACAGCATATCTTGGTGGTATTACCGAACCAGCCATGTTTGGTGTGA
ATCTGAAGTTAAAGAAGCCGATGTACGGTGCCATGATTGGTGGGGCAGTTGCCGGTTTGTTTGCCGGATTTATGAAAATG
AAAGCATTTATTTATGTCACTCCTGGACTACTCAGCTTACCAATGTGGGTATCTAAGACAGAAAACTACGTCGTATTGGC
AGTCGCTACCATTGTTATTGCTAGTGTGGCAACATTTATCGCAACTTGGTTGATTGGCTTCGAAGATCCTGTCAGCGAGG
AAGTTATGAAACAAAAGCAGGCGCAGGCGAACAAAGTGGTGACAACTAAACATTCGATTAATAGCCCCGTTGTTGGGGAA
GCTCGGATGTTGAATGAAGTTAATGATGAAACGTTTGCAAGTGGCGTTATGGGCAAAGGGATTGCTGTCATCCCAACTGA
GGGTGTCGTGATTGCCCCCACGGATGGTGTTGCATCAGCTGTCTTTGATACCAGCCATGCGATTGGCCTTCATTTAGATA
ATGATGCTGATTTGTTGATTCATGTGGGAATTGATACTGTTGAATTAAAAGGTCAATATTTTGAAACATTAGTAAAAAAG
GGTGAGCGCTTTCACGCAGGTCAAGAGTTATTACGGTTTGATTTAGACAAGATAAAACAGGCTGGATATGATCCAACGGT
TATGATTATTGTGTTAAATACAAAGGACTTTTTAGAGGTTCTACCAGTACCAGAATCGAACGAATCTGTAACCGCAGATA
ATAATTTATTAATGTTAGCGTAA

Upstream 100 bases:

>100_bases
TTGTTCAGAAGACCTATCACTATACGCTTAACAATGAAGATTTACTGTACATGACGATTCATATCGCAAGAATTGCGGAT
GCAAATTAGGGGGCAGAATA

Downstream 100 bases:

>100_bases
GTGAGGTAGGATTGATGGATGTTTACAAAACGAGTCCTAACTTTATGTGGGGCGTAGCAACTGCTGCTAATCAAGTAGAA
GGCGCATGGAACGAGGACGG

Product: beta-glucosides PTS, EIIBCA

Products: NA

Alternate protein names: EIIBCA-Bgl; EII-Bgl; Beta-glucoside-specific phosphotransferase enzyme IIB component; PTS system beta-glucoside-specific EIIB component; Beta-glucoside permease IIC component; PTS system beta-glucoside-specific EIIC component; Beta-glucoside-specific phosphotransferase enzyme IIA component; PTS system beta-glucoside-specific EIIA component [H]

Number of amino acids: Translated: 620; Mature: 619

Protein sequence:

>620_residues
MTYKEMNDQIINGVGGKSNIESVVHCATRLRFVLKDEAKADDDLVRSIPGILQVVKKAGQYQLVIGNNVEDVYNELSDML
QLDENVDHNQSKDKRSIFDKIISTITGSIAPAIPLLAGAGMGKVLLLILTLSGLLTEKSQTYQMLNLIFDTGYFFMPAFI
GFSAAKIFKTNQFLGAFMGLVTVNPNWVAMVAAGKPVSFIGVPIQLVSYSSTLITAILSVWLMSYIEKFVKKITPGMIKV
FAEPMLIMLITAPLTFIVLGPIANLISMGIASISMFLYDNAGFIAIPLLAAAYPWLVSIGIHKALSPISIQLVATQGFDP
IIRVVALCSNMSQAAASLAVGLKTKNKQLRALALSSTVTAYLGGITEPAMFGVNLKLKKPMYGAMIGGAVAGLFAGFMKM
KAFIYVTPGLLSLPMWVSKTENYVVLAVATIVIASVATFIATWLIGFEDPVSEEVMKQKQAQANKVVTTKHSINSPVVGE
ARMLNEVNDETFASGVMGKGIAVIPTEGVVIAPTDGVASAVFDTSHAIGLHLDNDADLLIHVGIDTVELKGQYFETLVKK
GERFHAGQELLRFDLDKIKQAGYDPTVMIIVLNTKDFLEVLPVPESNESVTADNNLLMLA

Sequences:

>Translated_620_residues
MTYKEMNDQIINGVGGKSNIESVVHCATRLRFVLKDEAKADDDLVRSIPGILQVVKKAGQYQLVIGNNVEDVYNELSDML
QLDENVDHNQSKDKRSIFDKIISTITGSIAPAIPLLAGAGMGKVLLLILTLSGLLTEKSQTYQMLNLIFDTGYFFMPAFI
GFSAAKIFKTNQFLGAFMGLVTVNPNWVAMVAAGKPVSFIGVPIQLVSYSSTLITAILSVWLMSYIEKFVKKITPGMIKV
FAEPMLIMLITAPLTFIVLGPIANLISMGIASISMFLYDNAGFIAIPLLAAAYPWLVSIGIHKALSPISIQLVATQGFDP
IIRVVALCSNMSQAAASLAVGLKTKNKQLRALALSSTVTAYLGGITEPAMFGVNLKLKKPMYGAMIGGAVAGLFAGFMKM
KAFIYVTPGLLSLPMWVSKTENYVVLAVATIVIASVATFIATWLIGFEDPVSEEVMKQKQAQANKVVTTKHSINSPVVGE
ARMLNEVNDETFASGVMGKGIAVIPTEGVVIAPTDGVASAVFDTSHAIGLHLDNDADLLIHVGIDTVELKGQYFETLVKK
GERFHAGQELLRFDLDKIKQAGYDPTVMIIVLNTKDFLEVLPVPESNESVTADNNLLMLA
>Mature_619_residues
TYKEMNDQIINGVGGKSNIESVVHCATRLRFVLKDEAKADDDLVRSIPGILQVVKKAGQYQLVIGNNVEDVYNELSDMLQ
LDENVDHNQSKDKRSIFDKIISTITGSIAPAIPLLAGAGMGKVLLLILTLSGLLTEKSQTYQMLNLIFDTGYFFMPAFIG
FSAAKIFKTNQFLGAFMGLVTVNPNWVAMVAAGKPVSFIGVPIQLVSYSSTLITAILSVWLMSYIEKFVKKITPGMIKVF
AEPMLIMLITAPLTFIVLGPIANLISMGIASISMFLYDNAGFIAIPLLAAAYPWLVSIGIHKALSPISIQLVATQGFDPI
IRVVALCSNMSQAAASLAVGLKTKNKQLRALALSSTVTAYLGGITEPAMFGVNLKLKKPMYGAMIGGAVAGLFAGFMKMK
AFIYVTPGLLSLPMWVSKTENYVVLAVATIVIASVATFIATWLIGFEDPVSEEVMKQKQAQANKVVTTKHSINSPVVGEA
RMLNEVNDETFASGVMGKGIAVIPTEGVVIAPTDGVASAVFDTSHAIGLHLDNDADLLIHVGIDTVELKGQYFETLVKKG
ERFHAGQELLRFDLDKIKQAGYDPTVMIIVLNTKDFLEVLPVPESNESVTADNNLLMLA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790159, Length=623, Percent_Identity=34.8314606741573, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI48994906, Length=481, Percent_Identity=36.1746361746362, Blast_Score=298, Evalue=1e-81,
Organism=Escherichia coli, GI2367362, Length=407, Percent_Identity=28.7469287469287, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788769, Length=476, Percent_Identity=26.2605042016807, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1786894, Length=123, Percent_Identity=38.2113821138211, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788757, Length=130, Percent_Identity=33.0769230769231, Blast_Score=90, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011297 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 66868; Mature: 66736

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS51093 PTS_EIIA_TYPE_1 ; PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYKEMNDQIINGVGGKSNIESVVHCATRLRFVLKDEAKADDDLVRSIPGILQVVKKAGQ
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
YQLVIGNNVEDVYNELSDMLQLDENVDHNQSKDKRSIFDKIISTITGSIAPAIPLLAGAG
EEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCC
MGKVLLLILTLSGLLTEKSQTYQMLNLIFDTGYFFMPAFIGFSAAKIFKTNQFLGAFMGL
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VTVNPNWVAMVAAGKPVSFIGVPIQLVSYSSTLITAILSVWLMSYIEKFVKKITPGMIKV
EEECCCEEEEEECCCCCEEECCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
FAEPMLIMLITAPLTFIVLGPIANLISMGIASISMFLYDNAGFIAIPLLAAAYPWLVSIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHH
IHKALSPISIQLVATQGFDPIIRVVALCSNMSQAAASLAVGLKTKNKQLRALALSSTVTA
HHHCCCCEEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
YLGGITEPAMFGVNLKLKKPMYGAMIGGAVAGLFAGFMKMKAFIYVTPGLLSLPMWVSKT
HHCCCCCCHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHCCHHHCCC
ENYVVLAVATIVIASVATFIATWLIGFEDPVSEEVMKQKQAQANKVVTTKHSINSPVVGE
CCEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCH
ARMLNEVNDETFASGVMGKGIAVIPTEGVVIAPTDGVASAVFDTSHAIGLHLDNDADLLI
HHHHHHCCHHHHHHHHCCCCEEEEECCCEEEECCCCHHHHHHCCCCEEEEEECCCCCEEE
HVGIDTVELKGQYFETLVKKGERFHAGQELLRFDLDKIKQAGYDPTVMIIVLNTKDFLEV
EECCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHC
LPVPESNESVTADNNLLMLA
CCCCCCCCCEECCCCEEEEC
>Mature Secondary Structure 
TYKEMNDQIINGVGGKSNIESVVHCATRLRFVLKDEAKADDDLVRSIPGILQVVKKAGQ
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
YQLVIGNNVEDVYNELSDMLQLDENVDHNQSKDKRSIFDKIISTITGSIAPAIPLLAGAG
EEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCC
MGKVLLLILTLSGLLTEKSQTYQMLNLIFDTGYFFMPAFIGFSAAKIFKTNQFLGAFMGL
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VTVNPNWVAMVAAGKPVSFIGVPIQLVSYSSTLITAILSVWLMSYIEKFVKKITPGMIKV
EEECCCEEEEEECCCCCEEECCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
FAEPMLIMLITAPLTFIVLGPIANLISMGIASISMFLYDNAGFIAIPLLAAAYPWLVSIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHH
IHKALSPISIQLVATQGFDPIIRVVALCSNMSQAAASLAVGLKTKNKQLRALALSSTVTA
HHHCCCCEEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
YLGGITEPAMFGVNLKLKKPMYGAMIGGAVAGLFAGFMKMKAFIYVTPGLLSLPMWVSKT
HHCCCCCCHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHCCHHHCCC
ENYVVLAVATIVIASVATFIATWLIGFEDPVSEEVMKQKQAQANKVVTTKHSINSPVVGE
CCEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCH
ARMLNEVNDETFASGVMGKGIAVIPTEGVVIAPTDGVASAVFDTSHAIGLHLDNDADLLI
HHHHHHCCHHHHHHHHCCCCEEEEECCCEEEECCCCHHHHHHCCCCEEEEEECCCCCEEE
HVGIDTVELKGQYFETLVKKGERFHAGQELLRFDLDKIKQAGYDPTVMIIVLNTKDFLEV
EECCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHC
LPVPESNESVTADNNLLMLA
CCCCCCCCCEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7883710; 7704263; 9384377; 8628237 [H]