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The map label for this gene is glpK

Identifier: 28377285

GI number: 28377285

Start: 338885

End: 340402

Strand: Direct

Name: glpK

Synonym: lp_0370

Alternate gene names: 28377285

Gene position: 338885-340402 (Clockwise)

Preceding gene: 28377284

Following gene: 28377286

Centisome position: 10.24

GC content: 48.55

Gene sequence:

>1518_bases
ATGACCGACAAGTACATTATGGCAATTGACGAAGGGACAACCAGTACCCGCGCCATTATCTTTGACCATGCGGGCCATAA
AGTGGCCGACTCACAACGGGAATTTCCACAGTATTTCCCGCAGCCGGGCTGGGTCGAACATAATGCCAATGAAATCTGGA
ATGCTGTCTTGTCTACTATTGCGAATGCCTTCATTGAATCGGGAATCAAACCCGCTCAAATTAGTGGTATTGGAATTACG
AATCAACGGGAAACAACCATCGTTTGGGACAAGCAAACTGGCTTGCCAATCTATAACGCAATCGTGTGGCAATCACGGCA
AACCGCGCCGATTGCTGAGAAGCTGGTTAAAGATGGCTATGGTGATTTGATTCATCAACATACGGGACTCGTCACAGACG
CTTACTTCTCGGCAACTAAAATTCGCTGGATCTTAGACCACGTGAAGGGGGCCCAAGAACGAGCCGAAAAGGGTGAATTA
CTCTTTGGAACGATCGATACGTGGCTCCTATGGAAACTTACCGGTGGGGCCACCCACGTGACGGATTATTCCAATGCCAG
TCGGACGATGCTCTTCAATATTCATGATTTGAAGTGGGACGACCAGATTTTGCAATTATTAAATATTCCAGCGGCGATGT
TGCCAGAAGTTCGCACTAATTCTGAAGTTTATGGCAAGACCAAGGACTATCATTTCTTTGGGTCGGAAGTTCCAATTAGT
GGGATGGCTGGTGATCAACAAGCGGCCTTGTTTGGTCAACTGGCGTTTGAACCGGGAACCGTGAAGAATACTTACGGGAC
TGGGGCTTTTATCGTGATGAACACCGGTGAAAAACCCCAATTATCCGATAATAATCTATTGACCACGATCGGTTACGGCA
TCAACGGTAAAGTGTACTACGCCTTGGAAGGGTCAATTTTCGTTGCCGGTTCAGCGATTCAGTGGTTGCGTGATGGGATT
CGCCTAGTTGAAAGCGCTCCCGATTCTGAACGGGCGGCGCGCGAATCGCATAATGAAAATGAAGTCTATGTGGTTCCGGC
CTTTACTGGTTTAGGGGCACCATATTGGGACTCTGAAGCACGTGGCTCAGTCTTTGGCTTGACGCGTGGCACGACTCGTG
AAGATTTTATTAAAGCAACCTTGCAAGCATTGGCTTACCAAACGCGGGACGTGGTTGAAACAATGAAGAAAGATTCCGGT
ATTGAGATTCCAGTATTAAAAGTTGACGGTGGCGCTGCTCGTAATGACTGGTTGATGCAATTTCAGGCGGATATCTTGGA
TACGCAAATTATGCGGGCTGCTAATCTGGAAACGACAGCGCTTGGTGCCGCCTTCTTGGCCGGGCTTAGCGTTGGTTATT
GGCAGGATTTGGAAGAATTAAAAGCGTCGTATAAGCCCGGCACGAGTTTTGACCCTGAGATGGGGCCAGCTGAACGGACG
AACTTATACGAGGGATGGCAGGCAGCGGTCAAAGCGACCCAAGTCTTTAAGCACACCCCATACCACGCAGGAAAATAG

Upstream 100 bases:

>100_bases
TATGCAAATCTATTCACAAAACGGTCGCAACATGGTATGATTCAATCATCAATTATGATAGCGTTTTCATAAAACTGTTA
TGACTAAGGAGTGACACACC

Downstream 100 bases:

>100_bases
AGGAGGACAACAACATGGCATTTTCAGCAACGACGCGTCAACAAAATATTGAACGCTTACAAAATGAAACGTTAGATTTA
CTCGTTATCGGTGGTGGGAT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase 1; Glycerokinase 1; GK 1

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MTDKYIMAIDEGTTSTRAIIFDHAGHKVADSQREFPQYFPQPGWVEHNANEIWNAVLSTIANAFIESGIKPAQISGIGIT
NQRETTIVWDKQTGLPIYNAIVWQSRQTAPIAEKLVKDGYGDLIHQHTGLVTDAYFSATKIRWILDHVKGAQERAEKGEL
LFGTIDTWLLWKLTGGATHVTDYSNASRTMLFNIHDLKWDDQILQLLNIPAAMLPEVRTNSEVYGKTKDYHFFGSEVPIS
GMAGDQQAALFGQLAFEPGTVKNTYGTGAFIVMNTGEKPQLSDNNLLTTIGYGINGKVYYALEGSIFVAGSAIQWLRDGI
RLVESAPDSERAARESHNENEVYVVPAFTGLGAPYWDSEARGSVFGLTRGTTREDFIKATLQALAYQTRDVVETMKKDSG
IEIPVLKVDGGAARNDWLMQFQADILDTQIMRAANLETTALGAAFLAGLSVGYWQDLEELKASYKPGTSFDPEMGPAERT
NLYEGWQAAVKATQVFKHTPYHAGK

Sequences:

>Translated_505_residues
MTDKYIMAIDEGTTSTRAIIFDHAGHKVADSQREFPQYFPQPGWVEHNANEIWNAVLSTIANAFIESGIKPAQISGIGIT
NQRETTIVWDKQTGLPIYNAIVWQSRQTAPIAEKLVKDGYGDLIHQHTGLVTDAYFSATKIRWILDHVKGAQERAEKGEL
LFGTIDTWLLWKLTGGATHVTDYSNASRTMLFNIHDLKWDDQILQLLNIPAAMLPEVRTNSEVYGKTKDYHFFGSEVPIS
GMAGDQQAALFGQLAFEPGTVKNTYGTGAFIVMNTGEKPQLSDNNLLTTIGYGINGKVYYALEGSIFVAGSAIQWLRDGI
RLVESAPDSERAARESHNENEVYVVPAFTGLGAPYWDSEARGSVFGLTRGTTREDFIKATLQALAYQTRDVVETMKKDSG
IEIPVLKVDGGAARNDWLMQFQADILDTQIMRAANLETTALGAAFLAGLSVGYWQDLEELKASYKPGTSFDPEMGPAERT
NLYEGWQAAVKATQVFKHTPYHAGK
>Mature_504_residues
TDKYIMAIDEGTTSTRAIIFDHAGHKVADSQREFPQYFPQPGWVEHNANEIWNAVLSTIANAFIESGIKPAQISGIGITN
QRETTIVWDKQTGLPIYNAIVWQSRQTAPIAEKLVKDGYGDLIHQHTGLVTDAYFSATKIRWILDHVKGAQERAEKGELL
FGTIDTWLLWKLTGGATHVTDYSNASRTMLFNIHDLKWDDQILQLLNIPAAMLPEVRTNSEVYGKTKDYHFFGSEVPISG
MAGDQQAALFGQLAFEPGTVKNTYGTGAFIVMNTGEKPQLSDNNLLTTIGYGINGKVYYALEGSIFVAGSAIQWLRDGIR
LVESAPDSERAARESHNENEVYVVPAFTGLGAPYWDSEARGSVFGLTRGTTREDFIKATLQALAYQTRDVVETMKKDSGI
EIPVLKVDGGAARNDWLMQFQADILDTQIMRAANLETTALGAAFLAGLSVGYWQDLEELKASYKPGTSFDPEMGPAERTN
LYEGWQAAVKATQVFKHTPYHAGK

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=496, Percent_Identity=44.3548387096774, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI42794763, Length=500, Percent_Identity=44, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI4504007, Length=496, Percent_Identity=44.3548387096774, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI41393575, Length=496, Percent_Identity=43.75, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI88196792, Length=507, Percent_Identity=34.1222879684418, Blast_Score=313, Evalue=2e-85,
Organism=Escherichia coli, GI1790361, Length=488, Percent_Identity=59.6311475409836, Blast_Score=611, Evalue=1e-176,
Organism=Escherichia coli, GI1790005, Length=502, Percent_Identity=22.7091633466135, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1787789, Length=476, Percent_Identity=23.109243697479, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789987, Length=466, Percent_Identity=23.175965665236, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=437, Percent_Identity=41.8764302059497, Blast_Score=357, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI71986140, Length=526, Percent_Identity=24.1444866920152, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71986147, Length=304, Percent_Identity=28.6184210526316, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321755, Length=549, Percent_Identity=32.7868852459016, Blast_Score=296, Evalue=5e-81,
Organism=Drosophila melanogaster, GI17864214, Length=504, Percent_Identity=40.4761904761905, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24655555, Length=493, Percent_Identity=37.9310344827586, Blast_Score=347, Evalue=8e-96,
Organism=Drosophila melanogaster, GI24655560, Length=493, Percent_Identity=37.9310344827586, Blast_Score=347, Evalue=8e-96,
Organism=Drosophila melanogaster, GI24655570, Length=493, Percent_Identity=37.9310344827586, Blast_Score=347, Evalue=8e-96,
Organism=Drosophila melanogaster, GI24655574, Length=493, Percent_Identity=37.9310344827586, Blast_Score=347, Evalue=8e-96,
Organism=Drosophila melanogaster, GI24655565, Length=493, Percent_Identity=37.9310344827586, Blast_Score=347, Evalue=8e-96,
Organism=Drosophila melanogaster, GI24654573, Length=447, Percent_Identity=40.4921700223714, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI19922058, Length=524, Percent_Identity=33.969465648855, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=34.4195519348269, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24656367, Length=492, Percent_Identity=27.6422764227642, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI21357867, Length=505, Percent_Identity=26.9306930693069, Blast_Score=214, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24656372, Length=325, Percent_Identity=29.8461538461538, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI45552909, Length=278, Percent_Identity=25.5395683453237, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK1_LACPL (Q88ZF1)

Other databases:

- EMBL:   AL935252
- RefSeq:   NP_784177.1
- ProteinModelPortal:   Q88ZF1
- SMR:   Q88ZF1
- GeneID:   1064209
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0370
- NMPDR:   fig|220668.1.peg.311
- HOGENOM:   HBG511469
- OMA:   VYGTTRG
- ProtClustDB:   PRK00047
- BioCyc:   LPLA220668:LP_0370-MONOMER
- BRENDA:   2.7.1.30
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55818; Mature: 55687

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 13-13 BINDING 17-17 BINDING 83-83 BINDING 135-135 BINDING 245-245 BINDING 267-267 BINDING 310-310

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKYIMAIDEGTTSTRAIIFDHAGHKVADSQREFPQYFPQPGWVEHNANEIWNAVLSTI
CCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
ANAFIESGIKPAQISGIGITNQRETTIVWDKQTGLPIYNAIVWQSRQTAPIAEKLVKDGY
HHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHEEECCCCCCHHHHHHHHCCH
GDLIHQHTGLVTDAYFSATKIRWILDHVKGAQERAEKGELLFGTIDTWLLWKLTGGATHV
HHHHHHHCCCEEHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEECCEEEEEEECCCEEEE
TDYSNASRTMLFNIHDLKWDDQILQLLNIPAAMLPEVRTNSEVYGKTKDYHFFGSEVPIS
ECCCCCCEEEEEEEECCCCHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCEEEECCCCCCC
GMAGDQQAALFGQLAFEPGTVKNTYGTGAFIVMNTGEKPQLSDNNLLTTIGYGINGKVYY
CCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCEEEE
ALEGSIFVAGSAIQWLRDGIRLVESAPDSERAARESHNENEVYVVPAFTGLGAPYWDSEA
EEECCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEEEHHCCCCCCCCCCC
RGSVFGLTRGTTREDFIKATLQALAYQTRDVVETMKKDSGIEIPVLKVDGGAARNDWLMQ
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHH
FQADILDTQIMRAANLETTALGAAFLAGLSVGYWQDLEELKASYKPGTSFDPEMGPAERT
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
NLYEGWQAAVKATQVFKHTPYHAGK
HHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDKYIMAIDEGTTSTRAIIFDHAGHKVADSQREFPQYFPQPGWVEHNANEIWNAVLSTI
CCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
ANAFIESGIKPAQISGIGITNQRETTIVWDKQTGLPIYNAIVWQSRQTAPIAEKLVKDGY
HHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHEEECCCCCCHHHHHHHHCCH
GDLIHQHTGLVTDAYFSATKIRWILDHVKGAQERAEKGELLFGTIDTWLLWKLTGGATHV
HHHHHHHCCCEEHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEECCEEEEEEECCCEEEE
TDYSNASRTMLFNIHDLKWDDQILQLLNIPAAMLPEVRTNSEVYGKTKDYHFFGSEVPIS
ECCCCCCEEEEEEEECCCCHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCEEEECCCCCCC
GMAGDQQAALFGQLAFEPGTVKNTYGTGAFIVMNTGEKPQLSDNNLLTTIGYGINGKVYY
CCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCEEEE
ALEGSIFVAGSAIQWLRDGIRLVESAPDSERAARESHNENEVYVVPAFTGLGAPYWDSEA
EEECCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEEEHHCCCCCCCCCCC
RGSVFGLTRGTTREDFIKATLQALAYQTRDVVETMKKDSGIEIPVLKVDGGAARNDWLMQ
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHH
FQADILDTQIMRAANLETTALGAAFLAGLSVGYWQDLEELKASYKPGTSFDPEMGPAERT
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
NLYEGWQAAVKATQVFKHTPYHAGK
HHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566