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The map label for this gene is gshR1 [H]

Identifier: 28377284

GI number: 28377284

Start: 337088

End: 338419

Strand: Direct

Name: gshR1 [H]

Synonym: lp_0369

Alternate gene names: 28377284

Gene position: 337088-338419 (Clockwise)

Preceding gene: 28377283

Following gene: 28377285

Centisome position: 10.19

GC content: 50.75

Gene sequence:

>1332_bases
ATGGCGGAACAGTACGATGTTGTTGTGATTGGTGGCGGACCAGCCGGCAATGCCATGGCTAGCGGATTAAAGGCCCAGGG
CAAGACAGTGTTGATCGTTGAAGCGGATCTGTGGGGCGGCACTTGTCCTAACCGCGGTTGTGACCCTAAGAAAATCCTGT
TAAGCGCCGTCGAAGCGCGACAAGCGGCGCAACATTTACAAGGGCAGGGCCTGATTGGCGCGCCCAAAATTGATTGGCCA
GCACTGATGGCGCATAAACGAGGCTATACGGATGGCATCAACGATGGGACGTTGAACGGACTAACGGGGCAAGATATTGC
GACGTTACATGGCCAAGCACACTTTCAATCCGACAATCAGTTAGCGGTCGGGGATCGAGTAGTCAGTGCCACTGATTACG
TGATTGCTACTGGTCAGCGTCCAGCGATTCTACCGATTACCGGGCACGAATACTTTAAGACGAGCACTGACTTCTTAGAT
TTGGACCAGATGCCTAAACGCGTGACATTTGTAGGTGGTGGCTACGTAGGCTTTGAATTGGCGACGATTGCGAATGCCGC
TGGCGCTGATGTGCACGTGATTCATCATAATGACCGCCCGTTAAAAGCTTTTGATGCAGATTTGGTTAAGGATTTGATGG
CCGCAATGACGGCTGATGGAATCACGTTTGACTTGAATACGGATGTCCAAGCAATTACTAAAACGGCGACCGGTCTACAA
TTGACAGCTGATAATTTCGAGCTGACAACGGATCTGGTCATCAGCTCAGCGGGACGGATTCCGAACGCGGACCAGTTAGG
TCTAGCCAACGTGGGCGTTACCTTTGATCGGCATGGGATTCAAGTCAACGATCATTTGCAGACGGCCAACCCGCACATTT
ATGCCATTGGGGATGTCAGCGATACACCGGTACCGAAGTTAACGCCAGTTGCAGGTTTTGAAGCGCGTTATCTGGTCGGT
GAGTTGACGCATCCTGGCGCAGCCATAAAGTATCCCGTTGTGCCAACGCAGGTTTTTGCAGCGCCCAAGTTAGCGCAAGT
CGGGATCAGCGCGGCCGTGGCGACTGAGCATCCAGATGAGTATCGTGTCAATACACTTGATATGACGAAGTGGTTCACTT
ATTACCGCTTTGGCGCACAACAAGCCCAAGCTAAAGTAGTGGTTGCTAAAGCGAGTGGGCAGGTTGTGGGTGCTACCCTT
CTAAGTGATGTTGCCGACGAGATGATTAACTACTTCACGTTGTTAATTGAAAAACACGTGACTTTACCAGATTTACAACG
GTTGGTATTGGCTTACCCAACGCCGGCTAGTGACTTACAATATTTGTATTAA

Upstream 100 bases:

>100_bases
GTCAACATGAAGAAGTATCTTAATCGTCAATTAAGGATGAATCAGTTACACTAGGTTGTGATAATTGATGGTCGCTAATG
AATTTAAGGAGGAATTTCGA

Downstream 100 bases:

>100_bases
TGTGTGGTTGAATTAAATCAAAGTCCAGCTATTCAGCTTAACTGAATATGTCAGCACTAACGGAGTGACCAGGAATGACC
GGCCACTCTTTTTTTGTTTG

Product: glutathione reductase

Products: NA

Alternate protein names: GR; GRase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MAEQYDVVVIGGGPAGNAMASGLKAQGKTVLIVEADLWGGTCPNRGCDPKKILLSAVEARQAAQHLQGQGLIGAPKIDWP
ALMAHKRGYTDGINDGTLNGLTGQDIATLHGQAHFQSDNQLAVGDRVVSATDYVIATGQRPAILPITGHEYFKTSTDFLD
LDQMPKRVTFVGGGYVGFELATIANAAGADVHVIHHNDRPLKAFDADLVKDLMAAMTADGITFDLNTDVQAITKTATGLQ
LTADNFELTTDLVISSAGRIPNADQLGLANVGVTFDRHGIQVNDHLQTANPHIYAIGDVSDTPVPKLTPVAGFEARYLVG
ELTHPGAAIKYPVVPTQVFAAPKLAQVGISAAVATEHPDEYRVNTLDMTKWFTYYRFGAQQAQAKVVVAKASGQVVGATL
LSDVADEMINYFTLLIEKHVTLPDLQRLVLAYPTPASDLQYLY

Sequences:

>Translated_443_residues
MAEQYDVVVIGGGPAGNAMASGLKAQGKTVLIVEADLWGGTCPNRGCDPKKILLSAVEARQAAQHLQGQGLIGAPKIDWP
ALMAHKRGYTDGINDGTLNGLTGQDIATLHGQAHFQSDNQLAVGDRVVSATDYVIATGQRPAILPITGHEYFKTSTDFLD
LDQMPKRVTFVGGGYVGFELATIANAAGADVHVIHHNDRPLKAFDADLVKDLMAAMTADGITFDLNTDVQAITKTATGLQ
LTADNFELTTDLVISSAGRIPNADQLGLANVGVTFDRHGIQVNDHLQTANPHIYAIGDVSDTPVPKLTPVAGFEARYLVG
ELTHPGAAIKYPVVPTQVFAAPKLAQVGISAAVATEHPDEYRVNTLDMTKWFTYYRFGAQQAQAKVVVAKASGQVVGATL
LSDVADEMINYFTLLIEKHVTLPDLQRLVLAYPTPASDLQYLY
>Mature_442_residues
AEQYDVVVIGGGPAGNAMASGLKAQGKTVLIVEADLWGGTCPNRGCDPKKILLSAVEARQAAQHLQGQGLIGAPKIDWPA
LMAHKRGYTDGINDGTLNGLTGQDIATLHGQAHFQSDNQLAVGDRVVSATDYVIATGQRPAILPITGHEYFKTSTDFLDL
DQMPKRVTFVGGGYVGFELATIANAAGADVHVIHHNDRPLKAFDADLVKDLMAAMTADGITFDLNTDVQAITKTATGLQL
TADNFELTTDLVISSAGRIPNADQLGLANVGVTFDRHGIQVNDHLQTANPHIYAIGDVSDTPVPKLTPVAGFEARYLVGE
LTHPGAAIKYPVVPTQVFAAPKLAQVGISAAVATEHPDEYRVNTLDMTKWFTYYRFGAQQAQAKVVVAKASGQVVGATLL
SDVADEMINYFTLLIEKHVTLPDLQRLVLAYPTPASDLQYLY

Specific function: Maintains high levels of reduced glutathione in the cytosol [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI50301238, Length=460, Percent_Identity=28.2608695652174, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI91199540, Length=449, Percent_Identity=28.2850779510022, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519430, Length=425, Percent_Identity=28.2352941176471, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI33519428, Length=425, Percent_Identity=28.2352941176471, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI33519426, Length=425, Percent_Identity=28.2352941176471, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI148277065, Length=425, Percent_Identity=28.2352941176471, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI148277071, Length=425, Percent_Identity=28.2352941176471, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI22035672, Length=429, Percent_Identity=28.9044289044289, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI291045266, Length=478, Percent_Identity=27.4058577405858, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI291045268, Length=348, Percent_Identity=29.5977011494253, Blast_Score=103, Evalue=5e-22,
Organism=Escherichia coli, GI1789915, Length=424, Percent_Identity=31.6037735849057, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1786307, Length=449, Percent_Identity=28.9532293986637, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI87082354, Length=461, Percent_Identity=25.3796095444685, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87081717, Length=453, Percent_Identity=25.60706401766, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789065, Length=249, Percent_Identity=28.5140562248996, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71983429, Length=427, Percent_Identity=29.9765807962529, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI71983419, Length=427, Percent_Identity=29.9765807962529, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI32565766, Length=446, Percent_Identity=27.5784753363229, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=25.7383966244726, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=436, Percent_Identity=24.0825688073394, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325166, Length=462, Percent_Identity=28.5714285714286, Blast_Score=159, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6321091, Length=460, Percent_Identity=23.9130434782609, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=23.0277185501066, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21358499, Length=458, Percent_Identity=27.9475982532751, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24640553, Length=475, Percent_Identity=30.1052631578947, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24640549, Length=475, Percent_Identity=30.1052631578947, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24640551, Length=475, Percent_Identity=30.1052631578947, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI17737741, Length=477, Percent_Identity=28.9308176100629, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 650 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006324
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.7 [H]

Molecular weight: Translated: 47281; Mature: 47150

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQYDVVVIGGGPAGNAMASGLKAQGKTVLIVEADLWGGTCPNRGCDPKKILLSAVEAR
CCCCEEEEEECCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
QAAQHLQGQGLIGAPKIDWPALMAHKRGYTDGINDGTLNGLTGQDIATLHGQAHFQSDNQ
HHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCEEECCCCE
LAVGDRVVSATDYVIATGQRPAILPITGHEYFKTSTDFLDLDQMPKRVTFVGGGYVGFEL
EEECCEEEECCCEEEECCCCCEEEEECCCHHHCCCCCEECCCCCCCEEEEEECCEEEEEH
ATIANAAGADVHVIHHNDRPLKAFDADLVKDLMAAMTADGITFDLNTDVQAITKTATGLQ
HHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCEE
LTADNFELTTDLVISSAGRIPNADQLGLANVGVTFDRHGIQVNDHLQTANPHIYAIGDVS
EECCCCEEEEHHHEECCCCCCCCCCCCEEEECEEEECCCEEECCCEECCCCEEEEECCCC
DTPVPKLTPVAGFEARYLVGELTHPGAAIKYPVVPTQVFAAPKLAQVGISAAVATEHPDE
CCCCCCCCCCCCCCEEEEEEECCCCCCEEECCCCCHHHHCCCCHHHHCCEEEEECCCCCC
YRVNTLDMTKWFTYYRFGAQQAQAKVVVAKASGQVVGATLLSDVADEMINYFTLLIEKHV
EEEEEEEHHHHHHHHHHCCCCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHCCC
TLPDLQRLVLAYPTPASDLQYLY
CCHHHHHHEEECCCCCCCCCCCC
>Mature Secondary Structure 
AEQYDVVVIGGGPAGNAMASGLKAQGKTVLIVEADLWGGTCPNRGCDPKKILLSAVEAR
CCCEEEEEECCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
QAAQHLQGQGLIGAPKIDWPALMAHKRGYTDGINDGTLNGLTGQDIATLHGQAHFQSDNQ
HHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCEEECCCCE
LAVGDRVVSATDYVIATGQRPAILPITGHEYFKTSTDFLDLDQMPKRVTFVGGGYVGFEL
EEECCEEEECCCEEEECCCCCEEEEECCCHHHCCCCCEECCCCCCCEEEEEECCEEEEEH
ATIANAAGADVHVIHHNDRPLKAFDADLVKDLMAAMTADGITFDLNTDVQAITKTATGLQ
HHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCEE
LTADNFELTTDLVISSAGRIPNADQLGLANVGVTFDRHGIQVNDHLQTANPHIYAIGDVS
EECCCCEEEEHHHEECCCCCCCCCCCCEEEECEEEECCCEEECCCEECCCCEEEEECCCC
DTPVPKLTPVAGFEARYLVGELTHPGAAIKYPVVPTQVFAAPKLAQVGISAAVATEHPDE
CCCCCCCCCCCCCCEEEEEEECCCCCCEEECCCCCHHHHCCCCHHHHCCEEEEECCCCCC
YRVNTLDMTKWFTYYRFGAQQAQAKVVVAKASGQVVGATLLSDVADEMINYFTLLIEKHV
EEEEEEEHHHHHHHHHHCCCCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHCCC
TLPDLQRLVLAYPTPASDLQYLY
CCHHHHHHEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7559423; 11759840 [H]