Click here to switch to the map view.

The map label for this gene is pts4ABC [H]

Identifier: 28377190

GI number: 28377190

Start: 237710

End: 239707

Strand: Direct

Name: pts4ABC [H]

Synonym: lp_0264

Alternate gene names: 28377190

Gene position: 237710-239707 (Clockwise)

Preceding gene: 28377189

Following gene: 28377191

Centisome position: 7.19

GC content: 47.7

Gene sequence:

>1998_bases
ATGGCAAAGGCACAAACAGTAACAATTTTGTCGCCGGTCAATGGGGACGTTATTGCGTTAAATAAGGTTAAAGACCCGGT
CTTTTCAGCCGGTATGATGGGACTGGGCTTTGGCGTTCAACCTTCAAGTGGAGAAGTTGTTGCACCAGTCAGTGGTCAGG
TCACGATGGTGGCTGAGACGAAACACGCGATTGGGTTCACAACGCCTGATGATCATTTGGAAGTATTAGTGCATCTCGGA
ATCGACACGGTCGACTTAAAGGGTGCCCCGTTCACGGTCAAAGTGGCCGCGGGCGATGATGTTCAGGCGGGGGACGTGAT
TGCCACCATGGATTTAGCTGCTATTCAAGCCGCTAATCGTCAGACCACAGTGATTTTAGCAGTGACTAACACTAGTGATA
AATTAGAATCGTTGGACGTCGTTACGGGGGCAAAAGCTGCCGGTGACGTGACAGCTACGGGCCGTCTGAACGCCAACACA
TTTAATGCTAAGCGGCAGAATCGATTACCAAAAGCGGGTAAATATGATCAGTTAGCCGCGGACATCGTGGCTAATGTTGG
TGGTGTCGAGAATGTTAGTAGTTTGATTCACTGCATTACTCGCTTGCGTTTTTATTTGAAAGATGAGAGTAAAGCCAATG
ATGACGTCGTGCGGGACTTAAAAGGGGTCATCGACGTAGCAAAAGCGGGCGGTCAATATCAGGTCGTGATTGGTCCTGCT
GTCACGGATGTCTATGATGCGATCATCAAGCAAATCGGACCGGGATTTTCAAATGATTCGGAGACGGCTAAAGCTGTTGC
TGAGACTGCCGCAGCAGCTCAGCGGCCAACGACGCCGTGGGGCAAAGTTAAATGGGGTATCAGTAATTTAATTGGTGTTA
TCACGGGTTCAATGATTCCCGTCATTGGATTATTAGCAGCCTCTGGTATTTTAAAAGGAATTCTTGCGTTACTTACCACG
TTTAATTTAGTTAGCCCGACCGCACCAACGTACGTGATTATTAATGCGATGGGCGACTCGGTCTTTTACTTCCTGCCAAT
CTTCGTCGGTTTTACGGCTGGTAAAAAGTTAGGGGCAGATCCGGTGATTATGGGAATCATCGGTGGGGTGCTGACTTATC
CAGCTATTGTAACGATGGCAAGTGGTAAAGCGACGAGTACGCTTTTAGGAATGGGGATCAACGCGAACTTCTTTGGCTTG
CCGGTACACGTTGCCTCGTATACGTATTCAATTTTCCCGATGATTGCGGGGGCCTGGCTAGCAAGTAAGTTGGAACCATG
GCTAAAACGGGTGATTCCAACGGTATTACGAATGATTTTTGTGCCATTATTTGAAGTTGTGATTGTTTCCGGTGCGATTC
TATTATTCCTTGGTCCAATTATTACTGTGCTTTCAACGGGATTGGCAAATATTATTGTGTGGGTCTACAATTTGAGCCCT
GCGATTTCTGGCTTAATCATTGGTGGCTTCTATCAAGTATTGGTGATCTTCGGGTTGCACTGGGCGGTTATTCCGATTGT
GGCCAACGATATTGCAACGACGGGTCACAGTTACTTGAACGCGATTGTTTCAGCGACGATGGTTGCCCAGGGGGGCGCAG
TATTCGCAATCGCCTTAAAGTCAAAGCTGGCTAACATTCAAGAACTTGCTTGGCCAGCAACGATTTCAGCCTTCTGTGGT
GTTACCGAACCAGCCATGTACGGGATCAACTTGAAATATGGTCGGGCCTTTGTGACTGCCAGTATTGGGGCGGCAGTTGG
TGGCTTCTTGACCGGCTTGATGAATGTCAATATGTGGGGCTTTACGGGTTCACTGATTGGCTTCACGTCCTTTGTTAATC
CGAAGGGCTTGGACTTTAGTTTTTATGGTTTCTTGATTGCATCCGCAGCCACGATTGTCGTGTCCTTCGCTTTAACTTGG
ATGTTTGGTTTTAAGGATGAGGATGCAAATGCAGTTAAAGTAGCGCCTAAAAAGCGACACTTAGGTGCAACCGCCTAG

Upstream 100 bases:

>100_bases
TTACTTTCCAATTATCAAGATGTTAATGTCACAGCACAATTGACGATGCAACCCTATCAAGCGGTTGCGCTGTTGTTAGG
TTAAGAATGGAGGAATGGGT

Downstream 100 bases:

>100_bases
TTAATGGCGACGTCAAATAATTAATCAACGTTAGGATAAACAAGTATTTTTCCGATTCGGAAAAATGCTTGTTTTTGATA
TTTAGTCGCCCTAACGAAGT

Product: beta-glucosides PTS, EIIABC

Products: NA

Alternate protein names: EIIBCA-Bgl; EII-Bgl; Beta-glucoside-specific phosphotransferase enzyme IIB component; PTS system beta-glucoside-specific EIIB component; Beta-glucoside permease IIC component; PTS system beta-glucoside-specific EIIC component; Beta-glucoside-specific phosphotransferase enzyme IIA component; PTS system beta-glucoside-specific EIIA component [H]

Number of amino acids: Translated: 665; Mature: 664

Protein sequence:

>665_residues
MAKAQTVTILSPVNGDVIALNKVKDPVFSAGMMGLGFGVQPSSGEVVAPVSGQVTMVAETKHAIGFTTPDDHLEVLVHLG
IDTVDLKGAPFTVKVAAGDDVQAGDVIATMDLAAIQAANRQTTVILAVTNTSDKLESLDVVTGAKAAGDVTATGRLNANT
FNAKRQNRLPKAGKYDQLAADIVANVGGVENVSSLIHCITRLRFYLKDESKANDDVVRDLKGVIDVAKAGGQYQVVIGPA
VTDVYDAIIKQIGPGFSNDSETAKAVAETAAAAQRPTTPWGKVKWGISNLIGVITGSMIPVIGLLAASGILKGILALLTT
FNLVSPTAPTYVIINAMGDSVFYFLPIFVGFTAGKKLGADPVIMGIIGGVLTYPAIVTMASGKATSTLLGMGINANFFGL
PVHVASYTYSIFPMIAGAWLASKLEPWLKRVIPTVLRMIFVPLFEVVIVSGAILLFLGPIITVLSTGLANIIVWVYNLSP
AISGLIIGGFYQVLVIFGLHWAVIPIVANDIATTGHSYLNAIVSATMVAQGGAVFAIALKSKLANIQELAWPATISAFCG
VTEPAMYGINLKYGRAFVTASIGAAVGGFLTGLMNVNMWGFTGSLIGFTSFVNPKGLDFSFYGFLIASAATIVVSFALTW
MFGFKDEDANAVKVAPKKRHLGATA

Sequences:

>Translated_665_residues
MAKAQTVTILSPVNGDVIALNKVKDPVFSAGMMGLGFGVQPSSGEVVAPVSGQVTMVAETKHAIGFTTPDDHLEVLVHLG
IDTVDLKGAPFTVKVAAGDDVQAGDVIATMDLAAIQAANRQTTVILAVTNTSDKLESLDVVTGAKAAGDVTATGRLNANT
FNAKRQNRLPKAGKYDQLAADIVANVGGVENVSSLIHCITRLRFYLKDESKANDDVVRDLKGVIDVAKAGGQYQVVIGPA
VTDVYDAIIKQIGPGFSNDSETAKAVAETAAAAQRPTTPWGKVKWGISNLIGVITGSMIPVIGLLAASGILKGILALLTT
FNLVSPTAPTYVIINAMGDSVFYFLPIFVGFTAGKKLGADPVIMGIIGGVLTYPAIVTMASGKATSTLLGMGINANFFGL
PVHVASYTYSIFPMIAGAWLASKLEPWLKRVIPTVLRMIFVPLFEVVIVSGAILLFLGPIITVLSTGLANIIVWVYNLSP
AISGLIIGGFYQVLVIFGLHWAVIPIVANDIATTGHSYLNAIVSATMVAQGGAVFAIALKSKLANIQELAWPATISAFCG
VTEPAMYGINLKYGRAFVTASIGAAVGGFLTGLMNVNMWGFTGSLIGFTSFVNPKGLDFSFYGFLIASAATIVVSFALTW
MFGFKDEDANAVKVAPKKRHLGATA
>Mature_664_residues
AKAQTVTILSPVNGDVIALNKVKDPVFSAGMMGLGFGVQPSSGEVVAPVSGQVTMVAETKHAIGFTTPDDHLEVLVHLGI
DTVDLKGAPFTVKVAAGDDVQAGDVIATMDLAAIQAANRQTTVILAVTNTSDKLESLDVVTGAKAAGDVTATGRLNANTF
NAKRQNRLPKAGKYDQLAADIVANVGGVENVSSLIHCITRLRFYLKDESKANDDVVRDLKGVIDVAKAGGQYQVVIGPAV
TDVYDAIIKQIGPGFSNDSETAKAVAETAAAAQRPTTPWGKVKWGISNLIGVITGSMIPVIGLLAASGILKGILALLTTF
NLVSPTAPTYVIINAMGDSVFYFLPIFVGFTAGKKLGADPVIMGIIGGVLTYPAIVTMASGKATSTLLGMGINANFFGLP
VHVASYTYSIFPMIAGAWLASKLEPWLKRVIPTVLRMIFVPLFEVVIVSGAILLFLGPIITVLSTGLANIIVWVYNLSPA
ISGLIIGGFYQVLVIFGLHWAVIPIVANDIATTGHSYLNAIVSATMVAQGGAVFAIALKSKLANIQELAWPATISAFCGV
TEPAMYGINLKYGRAFVTASIGAAVGGFLTGLMNVNMWGFTGSLIGFTSFVNPKGLDFSFYGFLIASAATIVVSFALTWM
FGFKDEDANAVKVAPKKRHLGATA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790159, Length=485, Percent_Identity=35.4639175257732, Blast_Score=298, Evalue=7e-82,
Organism=Escherichia coli, GI48994906, Length=488, Percent_Identity=26.844262295082, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI2367362, Length=436, Percent_Identity=28.8990825688073, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1788769, Length=454, Percent_Identity=27.3127753303965, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788757, Length=144, Percent_Identity=31.25, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786894, Length=125, Percent_Identity=36.8, Blast_Score=83, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011297 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 69491; Mature: 69360

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS51093 PTS_EIIA_TYPE_1 ; PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAQTVTILSPVNGDVIALNKVKDPVFSAGMMGLGFGVQPSSGEVVAPVSGQVTMVAET
CCCCEEEEEEECCCCCEEEEECCCCHHHHCCHHHCCCCCCCCCCCEEEECCCCEEEEEEC
KHAIGFTTPDDHLEVLVHLGIDTVDLKGAPFTVKVAAGDDVQAGDVIATMDLAAIQAANR
CCCCCCCCCCHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHCCC
QTTVILAVTNTSDKLESLDVVTGAKAAGDVTATGRLNANTFNAKRQNRLPKAGKYDQLAA
CEEEEEEEECCCHHHHHHHHHCCCCCCCCEEECCEECCCCCCCHHHHCCCCCCCHHHHHH
DIVANVGGVENVSSLIHCITRLRFYLKDESKANDDVVRDLKGVIDVAKAGGQYQVVIGPA
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCH
VTDVYDAIIKQIGPGFSNDSETAKAVAETAAAAQRPTTPWGKVKWGISNLIGVITGSMIP
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
VIGLLAASGILKGILALLTTFNLVSPTAPTYVIINAMGDSVFYFLPIFVGFTAGKKLGAD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCC
PVIMGIIGGVLTYPAIVTMASGKATSTLLGMGINANFFGLPVHVASYTYSIFPMIAGAWL
HHHHHHHHHHHHHHHHEEECCCCCHHHHEECCCCCCEEECEEEHHHHHHHHHHHHHHHHH
ASKLEPWLKRVIPTVLRMIFVPLFEVVIVSGAILLFLGPIITVLSTGLANIIVWVYNLSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCH
AISGLIIGGFYQVLVIFGLHWAVIPIVANDIATTGHSYLNAIVSATMVAQGGAVFAIALK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEHH
SKLANIQELAWPATISAFCGVTEPAMYGINLKYGRAFVTASIGAAVGGFLTGLMNVNMWG
HHHHHHHHHHCHHHHHHHCCCCCCCEEEEEEECCCEEEEHHHHHHHHHHHHHHHHCEECC
FTGSLIGFTSFVNPKGLDFSFYGFLIASAATIVVSFALTWMFGFKDEDANAVKVAPKKRH
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCC
LGATA
CCCCC
>Mature Secondary Structure 
AKAQTVTILSPVNGDVIALNKVKDPVFSAGMMGLGFGVQPSSGEVVAPVSGQVTMVAET
CCCEEEEEEECCCCCEEEEECCCCHHHHCCHHHCCCCCCCCCCCEEEECCCCEEEEEEC
KHAIGFTTPDDHLEVLVHLGIDTVDLKGAPFTVKVAAGDDVQAGDVIATMDLAAIQAANR
CCCCCCCCCCHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHCCC
QTTVILAVTNTSDKLESLDVVTGAKAAGDVTATGRLNANTFNAKRQNRLPKAGKYDQLAA
CEEEEEEEECCCHHHHHHHHHCCCCCCCCEEECCEECCCCCCCHHHHCCCCCCCHHHHHH
DIVANVGGVENVSSLIHCITRLRFYLKDESKANDDVVRDLKGVIDVAKAGGQYQVVIGPA
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCH
VTDVYDAIIKQIGPGFSNDSETAKAVAETAAAAQRPTTPWGKVKWGISNLIGVITGSMIP
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
VIGLLAASGILKGILALLTTFNLVSPTAPTYVIINAMGDSVFYFLPIFVGFTAGKKLGAD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCC
PVIMGIIGGVLTYPAIVTMASGKATSTLLGMGINANFFGLPVHVASYTYSIFPMIAGAWL
HHHHHHHHHHHHHHHHEEECCCCCHHHHEECCCCCCEEECEEEHHHHHHHHHHHHHHHHH
ASKLEPWLKRVIPTVLRMIFVPLFEVVIVSGAILLFLGPIITVLSTGLANIIVWVYNLSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCH
AISGLIIGGFYQVLVIFGLHWAVIPIVANDIATTGHSYLNAIVSATMVAQGGAVFAIALK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEHH
SKLANIQELAWPATISAFCGVTEPAMYGINLKYGRAFVTASIGAAVGGFLTGLMNVNMWG
HHHHHHHHHHCHHHHHHHCCCCCCCEEEEEEECCCEEEEHHHHHHHHHHHHHHHHCEECC
FTGSLIGFTSFVNPKGLDFSFYGFLIASAATIVVSFALTWMFGFKDEDANAVKVAPKKRH
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCC
LGATA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]