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The map label for this gene is pucF [H]

Identifier: 28211532

GI number: 28211532

Start: 2018610

End: 2019830

Strand: Direct

Name: pucF [H]

Synonym: CTC01898

Alternate gene names: 28211532

Gene position: 2018610-2019830 (Clockwise)

Preceding gene: 28211528

Following gene: 28211533

Centisome position: 72.11

GC content: 36.28

Gene sequence:

>1221_bases
ATGTTAATTTGTAATAAGAAAAGATTGGAAAATAAGATTCTTACCTTTAGTAAATTTGGTGCTACTAAAAAAGGTGGGAT
AACAAGGTTATCCTTATCAAAACCTGCCCTCCAGGCAAGAGCAGAATTCTGTAAAAGAATGGACACCCTAGGTGCAAATA
TCGTAACGGATGATATGGGAAATGTATATGCAACCTTTAGAGGTACAGAGAATCTCCCACATATTGCAATGGGATCACAT
TGCGATTCAGTAGTACAAGGTGGAAACTATGATGGTATTTTAGGTGTACTTACTGCAATGGAAGTAGCTGAAACCATAGT
TACAGAGAAGATTCCACATCGACATCCAATTACAGTTATGATTTGGACCAATGAAGAAGGAGCTCGTTTCGATCCTGCTA
TGATGTCATCAGGTGTTATTACTGGTAAATTTGATAAATCTAAGATGTTAGCTTCAAAAGATACAGAAGGACTAACCTTT
GGTGAAGCTTTGAATGCAAGTGGATACAAGGGTGACGAGAAGAACAGACTTAATCCTAAGGACTATACGGCTTTTGTAGA
ACTTCACATTGAGCAAGGCCCAGTGCTAGAATCCACAAAAATGGATATTGGTGTTGTGGAAGGTGTTGTTGGAATGGTTA
ACTATGAATTTGAATTTGTAGGTCAAGCCGGTCATGCTGGAACCGTTCCACAGAAAATGAGAAAAGATGCTCTTTTAGCA
GCTTCTGAGGCTATTCAATATCTGCATAGAGAACTTGACAAGCTTGATAGTAAATTAGTGTACACTACAGGTAGAATAAT
TTGTTCACCTAATGTACATACAATTATACCAGATAACGTTAAATTTACCTTAGATGCAAGACATCAAGACCCTAATGTAA
TACAACAGGTTGTTGAAGTTATAGAAAACATTCCTAAAGAACTTGCAAAATGTAAAGTTAGTTATAAAGAGCTCTGGTCT
CGTAAAACTGTAAGCTTTAATGATGAATTTGTTAACTTAGTTGAAAAAAATGCTAATATTTATGGCTACTCTAATATGAG
AATATATAGTGGTCCTGGACATGATGCTCAATTTGTGGCTGATATGCTACCTACAACCATGATATTTGTTCCAAGTATTG
GTGGACACAGCCATTGCGAAATAGAAGAAACCCCTCTTGATAACTGCTTAAAAGGTGCTAATGTATTACTACAAACCATC
TTGGATATAGATAAGAAGTAG

Upstream 100 bases:

>100_bases
ATAAGGATAGGAAAACATAAAATTAATATTATGCCTTGGACAATTATTCTAGGCATGGTATTAATTTTAAAAACTATTTA
TAAAAAGGAGTGTAGATAAT

Downstream 100 bases:

>100_bases
ATTGTGTTTAACTTCCTAAATTCCAATAAAAGTTTTATTTTTACTGGAATTGCATTTTTTATGTTAATATTTTTTCTCCC
TAAATAAAGAAGCACACTCT

Product: allantoate amidohydrolase

Products: NA

Alternate protein names: Allantoate deiminase [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MLICNKKRLENKILTFSKFGATKKGGITRLSLSKPALQARAEFCKRMDTLGANIVTDDMGNVYATFRGTENLPHIAMGSH
CDSVVQGGNYDGILGVLTAMEVAETIVTEKIPHRHPITVMIWTNEEGARFDPAMMSSGVITGKFDKSKMLASKDTEGLTF
GEALNASGYKGDEKNRLNPKDYTAFVELHIEQGPVLESTKMDIGVVEGVVGMVNYEFEFVGQAGHAGTVPQKMRKDALLA
ASEAIQYLHRELDKLDSKLVYTTGRIICSPNVHTIIPDNVKFTLDARHQDPNVIQQVVEVIENIPKELAKCKVSYKELWS
RKTVSFNDEFVNLVEKNANIYGYSNMRIYSGPGHDAQFVADMLPTTMIFVPSIGGHSHCEIEETPLDNCLKGANVLLQTI
LDIDKK

Sequences:

>Translated_406_residues
MLICNKKRLENKILTFSKFGATKKGGITRLSLSKPALQARAEFCKRMDTLGANIVTDDMGNVYATFRGTENLPHIAMGSH
CDSVVQGGNYDGILGVLTAMEVAETIVTEKIPHRHPITVMIWTNEEGARFDPAMMSSGVITGKFDKSKMLASKDTEGLTF
GEALNASGYKGDEKNRLNPKDYTAFVELHIEQGPVLESTKMDIGVVEGVVGMVNYEFEFVGQAGHAGTVPQKMRKDALLA
ASEAIQYLHRELDKLDSKLVYTTGRIICSPNVHTIIPDNVKFTLDARHQDPNVIQQVVEVIENIPKELAKCKVSYKELWS
RKTVSFNDEFVNLVEKNANIYGYSNMRIYSGPGHDAQFVADMLPTTMIFVPSIGGHSHCEIEETPLDNCLKGANVLLQTI
LDIDKK
>Mature_406_residues
MLICNKKRLENKILTFSKFGATKKGGITRLSLSKPALQARAEFCKRMDTLGANIVTDDMGNVYATFRGTENLPHIAMGSH
CDSVVQGGNYDGILGVLTAMEVAETIVTEKIPHRHPITVMIWTNEEGARFDPAMMSSGVITGKFDKSKMLASKDTEGLTF
GEALNASGYKGDEKNRLNPKDYTAFVELHIEQGPVLESTKMDIGVVEGVVGMVNYEFEFVGQAGHAGTVPQKMRKDALLA
ASEAIQYLHRELDKLDSKLVYTTGRIICSPNVHTIIPDNVKFTLDARHQDPNVIQQVVEVIENIPKELAKCKVSYKELWS
RKTVSFNDEFVNLVEKNANIYGYSNMRIYSGPGHDAQFVADMLPTTMIFVPSIGGHSHCEIEETPLDNCLKGANVLLQTI
LDIDKK

Specific function: Involved in the anaerobic utilization of allantoin. Converts allantoate to (S)-ureidoglycolate and ammonia [H]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI1786726, Length=400, Percent_Identity=33, Blast_Score=207, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010158
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF01546 Peptidase_M20 [H]

EC number: 3.5.3.- [C]

Molecular weight: Translated: 44890; Mature: 44890

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLICNKKRLENKILTFSKFGATKKGGITRLSLSKPALQARAEFCKRMDTLGANIVTDDMG
CCCCCHHHHHHHHEEHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCC
NVYATFRGTENLPHIAMGSHCDSVVQGGNYDGILGVLTAMEVAETIVTEKIPHRHPITVM
CEEEEEECCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
IWTNEEGARFDPAMMSSGVITGKFDKSKMLASKDTEGLTFGEALNASGYKGDEKNRLNPK
EEECCCCCCCCHHHHHCCCEEECCCCHHHHHCCCCCCCEEHHHCCCCCCCCCCCCCCCCC
DYTAFVELHIEQGPVLESTKMDIGVVEGVVGMVNYEFEFVGQAGHAGTVPQKMRKDALLA
CEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHH
ASEAIQYLHRELDKLDSKLVYTTGRIICSPNVHTIIPDNVKFTLDARHQDPNVIQQVVEV
HHHHHHHHHHHHHHHCCEEEEECCEEEECCCCCEECCCCEEEEEECCCCCHHHHHHHHHH
IENIPKELAKCKVSYKELWSRKTVSFNDEFVNLVEKNANIYGYSNMRIYSGPGHDAQFVA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEECCEEEECCCCCHHHHHH
DMLPTTMIFVPSIGGHSHCEIEETPLDNCLKGANVLLQTILDIDKK
HHHCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLICNKKRLENKILTFSKFGATKKGGITRLSLSKPALQARAEFCKRMDTLGANIVTDDMG
CCCCCHHHHHHHHEEHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCC
NVYATFRGTENLPHIAMGSHCDSVVQGGNYDGILGVLTAMEVAETIVTEKIPHRHPITVM
CEEEEEECCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
IWTNEEGARFDPAMMSSGVITGKFDKSKMLASKDTEGLTFGEALNASGYKGDEKNRLNPK
EEECCCCCCCCHHHHHCCCEEECCCCHHHHHCCCCCCCEEHHHCCCCCCCCCCCCCCCCC
DYTAFVELHIEQGPVLESTKMDIGVVEGVVGMVNYEFEFVGQAGHAGTVPQKMRKDALLA
CEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHH
ASEAIQYLHRELDKLDSKLVYTTGRIICSPNVHTIIPDNVKFTLDARHQDPNVIQQVVEV
HHHHHHHHHHHHHHHCCEEEEECCEEEECCCCCEECCCCEEEEEECCCCCHHHHHHHHHH
IENIPKELAKCKVSYKELWSRKTVSFNDEFVNLVEKNANIYGYSNMRIYSGPGHDAQFVA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEECCEEEECCCCCHHHHHH
DMLPTTMIFVPSIGGHSHCEIEETPLDNCLKGANVLLQTILDIDKK
HHHCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]