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The map label for this gene is yeiT [H]

Identifier: 28210532

GI number: 28210532

Start: 862143

End: 863414

Strand: Direct

Name: yeiT [H]

Synonym: CTC00815

Alternate gene names: 28210532

Gene position: 862143-863414 (Clockwise)

Preceding gene: 28210531

Following gene: 28210533

Centisome position: 30.8

GC content: 30.58

Gene sequence:

>1272_bases
ATGGAAAAAAATAATTATTCAAAGGAAGTTAAAAAAACTTATACTCCACTACTAGCAATAGAGGAAGCTTCAAGATGTCT
TTTATGTTATGATGCACCCTGTAGTAAGAATTGTCCTGTAGATACGAAACCAGATGAATTCATAAGATCTTTAAGATTTA
AAAATTTAAAAGGGGCAGCAGAAACCATCAGAAAAAATAATATATTAGGTGGAGTATGCTCAAGACTTTGTCCTTATGAT
AAATATTGTCAAAAAAATTGTACAAGAGGAAAAATTGATAAGCCTATAGATATAGGATCACTTCAAAGATATTTAATAGA
ATTTGAGAAAAACATAAGATTAGATACGTTAAATAAAGTTGAAATTAATAAGGAAAAGGTGGCCATTATAGGATCGGGTC
CTGGAGGATTATCAGCGGCTGCATCTTTAGCTCAAAAAGGATATAAAGTTAATATTTTTGAGAAGAAAGAAAAATTTGGT
GGTTGGTTAACTTATGGAATACCAGAATACAGATTACCACAAAATTTAGTGGATTATGAGATTGATATTATAAGGAAATT
AGGGGTGGAATTTAAGGCTAATTGTAATGTAGGTAAAGATATTACGATAGACGATTTAAAAGAGAAAGGATTTGAAGCTA
TTCTATTAGCTTGTGGACAACAACAAAGTAAAGAATTAAACATAGAAGGAATAAAGTTAAAAGGAGTATTAAAGGGAACA
GAATTTTTATCTGAGGCTAAATTAAGTAATGGGAACTTAGAAGTAGGGCAAAAGGTTATAGTTATAGGTGGTGGAGATGT
GGCTATAGATTCCGCAGTAACAGCTAAATTACTTGGAGCGGAGGATGTGAAAATAGTATATAGAAGAAATATAGAAAAAA
TGCCCTCAAATAAAAATGAGAGAATCTATGCACATAATTTAAATATACCTATATTTACGGGAATAAAGCCTTATAGCTTA
ATAGGAAATAATGAGAAGGTTATTAGTTTTAAAGGTGTAGGGATGTTTGATGATTCTAGTTTAGAATTGGAAGTGGATAC
AGTTATATTTGCTATAGGGCAAGAATTAGAAGATCTTTCCTATATAGATGAATTAAAAATTGAAAATAATGAGGTTCTGG
TGGATGAAAATTTAAATACCAGCATAGAAGGAATATTTGCCTGTGGAGATATTATAAAAGGAAATAAAACAGTAGTGGAT
GCAGTTGCAACGGGTAAAAGAGTTGCTATAGAAATAGAAGAATATATAAGAAGAAAGGGAGATGTTAGGTAA

Upstream 100 bases:

>100_bases
GTAATAAAATGTAATATTAATTTATTTACAAAATATATATAGAATAGGGAAAAAACCTTTAATATAATCTGATTTTTAAC
AAATATTTGGAGGGGAACAG

Downstream 100 bases:

>100_bases
TGAAAAAAGTAGATTTATCCATTGAATTTTGTGGGGTGAAATGTGAAAATCCATTTTTCTTATCATCTTCTCCTGTTGGA
CACAATTATGAAATGTGTGC

Product: putative NADPH-dependent glutamate synthase beta chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MEKNNYSKEVKKTYTPLLAIEEASRCLLCYDAPCSKNCPVDTKPDEFIRSLRFKNLKGAAETIRKNNILGGVCSRLCPYD
KYCQKNCTRGKIDKPIDIGSLQRYLIEFEKNIRLDTLNKVEINKEKVAIIGSGPGGLSAAASLAQKGYKVNIFEKKEKFG
GWLTYGIPEYRLPQNLVDYEIDIIRKLGVEFKANCNVGKDITIDDLKEKGFEAILLACGQQQSKELNIEGIKLKGVLKGT
EFLSEAKLSNGNLEVGQKVIVIGGGDVAIDSAVTAKLLGAEDVKIVYRRNIEKMPSNKNERIYAHNLNIPIFTGIKPYSL
IGNNEKVISFKGVGMFDDSSLELEVDTVIFAIGQELEDLSYIDELKIENNEVLVDENLNTSIEGIFACGDIIKGNKTVVD
AVATGKRVAIEIEEYIRRKGDVR

Sequences:

>Translated_423_residues
MEKNNYSKEVKKTYTPLLAIEEASRCLLCYDAPCSKNCPVDTKPDEFIRSLRFKNLKGAAETIRKNNILGGVCSRLCPYD
KYCQKNCTRGKIDKPIDIGSLQRYLIEFEKNIRLDTLNKVEINKEKVAIIGSGPGGLSAAASLAQKGYKVNIFEKKEKFG
GWLTYGIPEYRLPQNLVDYEIDIIRKLGVEFKANCNVGKDITIDDLKEKGFEAILLACGQQQSKELNIEGIKLKGVLKGT
EFLSEAKLSNGNLEVGQKVIVIGGGDVAIDSAVTAKLLGAEDVKIVYRRNIEKMPSNKNERIYAHNLNIPIFTGIKPYSL
IGNNEKVISFKGVGMFDDSSLELEVDTVIFAIGQELEDLSYIDELKIENNEVLVDENLNTSIEGIFACGDIIKGNKTVVD
AVATGKRVAIEIEEYIRRKGDVR
>Mature_423_residues
MEKNNYSKEVKKTYTPLLAIEEASRCLLCYDAPCSKNCPVDTKPDEFIRSLRFKNLKGAAETIRKNNILGGVCSRLCPYD
KYCQKNCTRGKIDKPIDIGSLQRYLIEFEKNIRLDTLNKVEINKEKVAIIGSGPGGLSAAASLAQKGYKVNIFEKKEKFG
GWLTYGIPEYRLPQNLVDYEIDIIRKLGVEFKANCNVGKDITIDDLKEKGFEAILLACGQQQSKELNIEGIKLKGVLKGT
EFLSEAKLSNGNLEVGQKVIVIGGGDVAIDSAVTAKLLGAEDVKIVYRRNIEKMPSNKNERIYAHNLNIPIFTGIKPYSL
IGNNEKVISFKGVGMFDDSSLELEVDTVIFAIGQELEDLSYIDELKIENNEVLVDENLNTSIEGIFACGDIIKGNKTVVD
AVATGKRVAIEIEEYIRRKGDVR

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=439, Percent_Identity=31.4350797266515, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI237757300, Length=90, Percent_Identity=42.2222222222222, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1788468, Length=416, Percent_Identity=42.5480769230769, Blast_Score=341, Evalue=5e-95,
Organism=Escherichia coli, GI1789606, Length=443, Percent_Identity=30.0225733634311, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI87082180, Length=447, Percent_Identity=26.6219239373602, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1788811, Length=466, Percent_Identity=28.1115879828326, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1789243, Length=431, Percent_Identity=31.0904872389791, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI71984108, Length=453, Percent_Identity=30.0220750551876, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17570289, Length=453, Percent_Identity=27.1523178807947, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17543860, Length=162, Percent_Identity=32.7160493827161, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=465, Percent_Identity=27.741935483871, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24640763, Length=445, Percent_Identity=30.7865168539326, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI18858217, Length=445, Percent_Identity=30.7865168539326, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24665547, Length=441, Percent_Identity=24.9433106575964, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24665543, Length=441, Percent_Identity=24.9433106575964, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI28574881, Length=446, Percent_Identity=25.1121076233184, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24665539, Length=446, Percent_Identity=25.1121076233184, Blast_Score=131, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 47023; Mature: 47023

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKNNYSKEVKKTYTPLLAIEEASRCLLCYDAPCSKNCPVDTKPDEFIRSLRFKNLKGAA
CCCCCHHHHHHHHHCCEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHH
ETIRKNNILGGVCSRLCPYDKYCQKNCTRGKIDKPIDIGSLQRYLIEFEKNIRLDTLNKV
HHHHHCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCEE
EINKEKVAIIGSGPGGLSAAASLAQKGYKVNIFEKKEKFGGWLTYGIPEYRLPQNLVDYE
EECCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECHHHCCCEEEECCCCCCCCCHHHHHH
IDIIRKLGVEFKANCNVGKDITIDDLKEKGFEAILLACGQQQSKELNIEGIKLKGVLKGT
HHHHHHCCCCEEECCCCCCCCCHHHHHHCCHHEEHHHCCCCCCCCCCCEEEEEEEEECHH
EFLSEAKLSNGNLEVGQKVIVIGGGDVAIDSAVTAKLLGAEDVKIVYRRNIEKMPSNKNE
HHHHHHHCCCCCEECCCEEEEECCCCEEECHHHHHHHCCCCCEEEEECCCHHHCCCCCCC
RIYAHNLNIPIFTGIKPYSLIGNNEKVISFKGVGMFDDSSLELEVDTVIFAIGQELEDLS
EEEEEECCCEEEECCCCEEEECCCCEEEEEECCCEECCCCCEEEEHHEEEEHHHHHHHHH
YIDELKIENNEVLVDENLNTSIEGIFACGDIIKGNKTVVDAVATGKRVAIEIEEYIRRKG
HHHHEEECCCEEEEECCCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEEHHHHHHHCC
DVR
CCC
>Mature Secondary Structure
MEKNNYSKEVKKTYTPLLAIEEASRCLLCYDAPCSKNCPVDTKPDEFIRSLRFKNLKGAA
CCCCCHHHHHHHHHCCEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHH
ETIRKNNILGGVCSRLCPYDKYCQKNCTRGKIDKPIDIGSLQRYLIEFEKNIRLDTLNKV
HHHHHCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCEE
EINKEKVAIIGSGPGGLSAAASLAQKGYKVNIFEKKEKFGGWLTYGIPEYRLPQNLVDYE
EECCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECHHHCCCEEEECCCCCCCCCHHHHHH
IDIIRKLGVEFKANCNVGKDITIDDLKEKGFEAILLACGQQQSKELNIEGIKLKGVLKGT
HHHHHHCCCCEEECCCCCCCCCHHHHHHCCHHEEHHHCCCCCCCCCCCEEEEEEEEECHH
EFLSEAKLSNGNLEVGQKVIVIGGGDVAIDSAVTAKLLGAEDVKIVYRRNIEKMPSNKNE
HHHHHHHCCCCCEECCCEEEEECCCCEEECHHHHHHHCCCCCEEEEECCCHHHCCCCCCC
RIYAHNLNIPIFTGIKPYSLIGNNEKVISFKGVGMFDDSSLELEVDTVIFAIGQELEDLS
EEEEEECCCEEEECCCCEEEECCCCEEEEEECCCEECCCCCEEEEHHEEEEHHHHHHHHH
YIDELKIENNEVLVDENLNTSIEGIFACGDIIKGNKTVVDAVATGKRVAIEIEEYIRRKG
HHHHEEECCCEEEEECCCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEEHHHHHHHCC
DVR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]