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The map label for this gene is ddl

Identifier: 28210407

GI number: 28210407

Start: 700107

End: 701012

Strand: Direct

Name: ddl

Synonym: CTC00683

Alternate gene names: 28210407

Gene position: 700107-701012 (Clockwise)

Preceding gene: 28210405

Following gene: 28210408

Centisome position: 25.01

GC content: 29.47

Gene sequence:

>906_bases
ATGCAAATAGGAGTAGTTATGGGAGGAGTATCTACAGAAAAAGAGATATCAAATCTTACGGGTAAATATATAATAGAGAA
CTTAGATAAAAGTAAATATGAAATACTCCCTATACCCATAAATACAAAGTTTGAATTAATAGATAAAATAAAGTGTTTAG
AATTTGCTTTTATTGCATTGCATGGTAATTTTGGAGAAGATGGAAAAGTTCAAGCCCTTTTAGAAACCATGGGAGTACCT
TATTCAGGATCAGGAGTATTGGCAAGTTCTCTTTGTATGGACAAAAATATGAGCAAAAAAATACTGCAAGGAGAGGGGAT
TAAAACTCCAAGGTGGATAATGCTATATAAAAATGAAGATATAGACTTTGAGAGTATTAAAAATATAGGTTACCCCCTAG
TAGTAAAGCCAAATAGTGGTGGATCCAGTATTGGTATAACTATAGTTAAAAAAGAACAAGAATTAATAAAAGCTATAGAA
GAAGCTTTTAAATTTGATGAAGAAATTTTAATAGAGGAATATATAAAAGGAGAAGAGATTACCTGTTGTATGTTAGATGG
CAAGCCACTTCCTATATTATCTATAAAAACAAAGGAAGAATTTTTTAATTACAAAGCTAAGTATTTTGAAGGGGTAGCAG
AGGAGAAGGTTGCAGATCTTTCTTTAGAATTAAAAGACCAAGTTGAGAAAATAAGTAATAAATGTTGGAAAAGTTTTAAG
TTGAAGGTTTATGGTAGAATTGATATGATTATAAAAGGGAATGAAGTATATGTAATAGAGATAAATACTTTACCAGGTAT
GACTAAATATAGCCTCTTTCCCAAAAGTGCTAAAGCTTATGGCTTAAATTTTAGTGAACTTTTAGATAAGATAATAGAAC
TTTCCATTAAAGAATATGAATTTTGA

Upstream 100 bases:

>100_bases
ATCCACATGTTATAAAAAAATAGATCATGGTGGTTCTTTCCGTAATGGAATAATACTAATACAATATATATTGTATTAGT
ATTTTTTTAGGAGGATTTTT

Downstream 100 bases:

>100_bases
GGTGTTTTTATGTTTCCTATTTTTAATATAAAGGATGGTCAGATAGCCTATGAAGAAATATATAAATACTTAAAAGAAAT
TATAAAAAATGGAATGATGC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase A; D-alanylalanine synthetase A

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MQIGVVMGGVSTEKEISNLTGKYIIENLDKSKYEILPIPINTKFELIDKIKCLEFAFIALHGNFGEDGKVQALLETMGVP
YSGSGVLASSLCMDKNMSKKILQGEGIKTPRWIMLYKNEDIDFESIKNIGYPLVVKPNSGGSSIGITIVKKEQELIKAIE
EAFKFDEEILIEEYIKGEEITCCMLDGKPLPILSIKTKEEFFNYKAKYFEGVAEEKVADLSLELKDQVEKISNKCWKSFK
LKVYGRIDMIIKGNEVYVIEINTLPGMTKYSLFPKSAKAYGLNFSELLDKIIELSIKEYEF

Sequences:

>Translated_301_residues
MQIGVVMGGVSTEKEISNLTGKYIIENLDKSKYEILPIPINTKFELIDKIKCLEFAFIALHGNFGEDGKVQALLETMGVP
YSGSGVLASSLCMDKNMSKKILQGEGIKTPRWIMLYKNEDIDFESIKNIGYPLVVKPNSGGSSIGITIVKKEQELIKAIE
EAFKFDEEILIEEYIKGEEITCCMLDGKPLPILSIKTKEEFFNYKAKYFEGVAEEKVADLSLELKDQVEKISNKCWKSFK
LKVYGRIDMIIKGNEVYVIEINTLPGMTKYSLFPKSAKAYGLNFSELLDKIIELSIKEYEF
>Mature_301_residues
MQIGVVMGGVSTEKEISNLTGKYIIENLDKSKYEILPIPINTKFELIDKIKCLEFAFIALHGNFGEDGKVQALLETMGVP
YSGSGVLASSLCMDKNMSKKILQGEGIKTPRWIMLYKNEDIDFESIKNIGYPLVVKPNSGGSSIGITIVKKEQELIKAIE
EAFKFDEEILIEEYIKGEEITCCMLDGKPLPILSIKTKEEFFNYKAKYFEGVAEEKVADLSLELKDQVEKISNKCWKSFK
LKVYGRIDMIIKGNEVYVIEINTLPGMTKYSLFPKSAKAYGLNFSELLDKIIELSIKEYEF

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=304, Percent_Identity=36.8421052631579, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI1786579, Length=352, Percent_Identity=29.8295454545455, Blast_Score=181, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDLA_CLOTE (Q897P8)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781351.1
- ProteinModelPortal:   Q897P8
- SMR:   Q897P8
- GeneID:   1058953
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00683
- NMPDR:   fig|212717.1.peg.573
- HOGENOM:   HBG427092
- OMA:   GNEMIAH
- PhylomeDB:   Q897P8
- ProtClustDB:   PRK01372
- BioCyc:   CTET212717:CTC_00683-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 34031; Mature: 34031

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGVVMGGVSTEKEISNLTGKYIIENLDKSKYEILPIPINTKFELIDKIKCLEFAFIAL
CEEEEEECCCCCHHHHHHCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHEEEEEEE
HGNFGEDGKVQALLETMGVPYSGSGVLASSLCMDKNMSKKILQGEGIKTPRWIMLYKNED
ECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEEECCC
IDFESIKNIGYPLVVKPNSGGSSIGITIVKKEQELIKAIEEAFKFDEEILIEEYIKGEEI
CCHHHHHHCCCEEEEEECCCCCEEEEEEEECHHHHHHHHHHHHCCCHHHHHHHHCCCCCE
TCCMLDGKPLPILSIKTKEEFFNYKAKYFEGVAEEKVADLSLELKDQVEKISNKCWKSFK
EEEEECCCCCEEEEEECHHHHHHHHHHHHHCHHHHHHHHCEEHHHHHHHHHHHHHHHHEE
LKVYGRIDMIIKGNEVYVIEINTLPGMTKYSLFPKSAKAYGLNFSELLDKIIELSIKEYE
EEEEEEEEEEEECCEEEEEEEECCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCC
F
C
>Mature Secondary Structure
MQIGVVMGGVSTEKEISNLTGKYIIENLDKSKYEILPIPINTKFELIDKIKCLEFAFIAL
CEEEEEECCCCCHHHHHHCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHEEEEEEE
HGNFGEDGKVQALLETMGVPYSGSGVLASSLCMDKNMSKKILQGEGIKTPRWIMLYKNED
ECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEEECCC
IDFESIKNIGYPLVVKPNSGGSSIGITIVKKEQELIKAIEEAFKFDEEILIEEYIKGEEI
CCHHHHHHCCCEEEEEECCCCCEEEEEEEECHHHHHHHHHHHHCCCHHHHHHHHCCCCCE
TCCMLDGKPLPILSIKTKEEFFNYKAKYFEGVAEEKVADLSLELKDQVEKISNKCWKSFK
EEEEECCCCCEEEEEECHHHHHHHHHHHHHCHHHHHHHHCEEHHHHHHHHHHHHHHHHEE
LKVYGRIDMIIKGNEVYVIEINTLPGMTKYSLFPKSAKAYGLNFSELLDKIIELSIKEYE
EEEEEEEEEEEECCEEEEEEEECCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129