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The map label for this gene is llaDCHIA [H]

Identifier: 28210142

GI number: 28210142

Start: 380624

End: 381466

Strand: Direct

Name: llaDCHIA [H]

Synonym: CTC00387

Alternate gene names: 28210142

Gene position: 380624-381466 (Clockwise)

Preceding gene: 28210141

Following gene: 28210143

Centisome position: 13.6

GC content: 27.05

Gene sequence:

>843_bases
ATGAAAAAGAATAAAATAATATTAGCAGCACCTGTATTGAAATGGGTAGGTGGAAAAAGACAGTTGATGGGTGAGATAAA
GAAGGTATTGCCAAAAACATATACTGCATATTATGAGCCATTTATAGGTGGAGGAGCAGTATTATTTGAATTACAACCTA
ACAAAGCTGTTATTAATGATGTTAATGGTGAATTAATAAATTTATACAATATAATTAAATACGATGTAGAATCATTAATA
GAAGATTTGAGAAAACATGAAAATACATCAGAATATTTTTATAGAATTAGAGAGATTGATAGAAGTAAAGAGGAGTATGA
AAAATTAACTAATGTTGAAAAGGCATCGAGAATAATTTATTTAAATAAAACTTGTTTTAATGGATTATTTAGGGTTAATA
AAGCAGGAGAATTTAATTCTCCATTTGGTAAATATAAAAATCCTAATATAGTTGATGAAGTAACGCTTAGAGCTGTAAAT
AAGTATTTTAATAAAGCAAATATAAAAATTTTGAATGGCGATTTTGAACAGAGTTTAAAGAGAATAAGAAAAGGTGCTTT
TGTGTATTTAGATCCACCGTATGATCCAGTTTCTAGTAGTGCTAATTTTACGGGATATGATAAAGGTGGATTTAACAGGG
CAGAACAAATAAGGTTAAAAAATTTATGTGATAAGTTAGACAAAAAAGGAGTAAAATTTTTATTATCAAATTCTGCAACA
GATTTTATTAAAGAACTATATAAAGATTATAATATAAAAGTAGTCAAAGCTAAACGAACTATAAATTCAAATGGTAATTC
TAGAGGGGCGGTAGATGAAGTATTGGTGAGAAATTATGAGTAA

Upstream 100 bases:

>100_bases
TGTACGTAATATTAAATTTTAAAGGATAATTTAAAAAAACATGTGTTCTGTTCAGAGAATTTGATGTATACTTAAATATA
TAATACTTTGGAGGAATAAT

Downstream 100 bases:

>100_bases
GAATAAAAATGATGTTGCATGGGAAACCCTATTCAATAAGTATAATATAATTTCTGAGATTGATAAGAATGGAAAATTTG
AAATATCAGCAAAAGAAATT

Product: putative adenine-specific DNA methyltransferase

Products: NA

Alternate protein names: M.LlaDCHI A; M.LlaDCHIA; Adenine-specific methyltransferase LlaDCHIA; M.LlaII A [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKKNKIILAAPVLKWVGGKRQLMGEIKKVLPKTYTAYYEPFIGGGAVLFELQPNKAVINDVNGELINLYNIIKYDVESLI
EDLRKHENTSEYFYRIREIDRSKEEYEKLTNVEKASRIIYLNKTCFNGLFRVNKAGEFNSPFGKYKNPNIVDEVTLRAVN
KYFNKANIKILNGDFEQSLKRIRKGAFVYLDPPYDPVSSSANFTGYDKGGFNRAEQIRLKNLCDKLDKKGVKFLLSNSAT
DFIKELYKDYNIKVVKAKRTINSNGNSRGAVDEVLVRNYE

Sequences:

>Translated_280_residues
MKKNKIILAAPVLKWVGGKRQLMGEIKKVLPKTYTAYYEPFIGGGAVLFELQPNKAVINDVNGELINLYNIIKYDVESLI
EDLRKHENTSEYFYRIREIDRSKEEYEKLTNVEKASRIIYLNKTCFNGLFRVNKAGEFNSPFGKYKNPNIVDEVTLRAVN
KYFNKANIKILNGDFEQSLKRIRKGAFVYLDPPYDPVSSSANFTGYDKGGFNRAEQIRLKNLCDKLDKKGVKFLLSNSAT
DFIKELYKDYNIKVVKAKRTINSNGNSRGAVDEVLVRNYE
>Mature_280_residues
MKKNKIILAAPVLKWVGGKRQLMGEIKKVLPKTYTAYYEPFIGGGAVLFELQPNKAVINDVNGELINLYNIIKYDVESLI
EDLRKHENTSEYFYRIREIDRSKEEYEKLTNVEKASRIIYLNKTCFNGLFRVNKAGEFNSPFGKYKNPNIVDEVTLRAVN
KYFNKANIKILNGDFEQSLKRIRKGAFVYLDPPYDPVSSSANFTGYDKGGFNRAEQIRLKNLCDKLDKKGVKFLLSNSAT
DFIKELYKDYNIKVVKAKRTINSNGNSRGAVDEVLVRNYE

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=281, Percent_Identity=34.5195729537367, Blast_Score=170, Evalue=8e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32216; Mature: 32216

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNKIILAAPVLKWVGGKRQLMGEIKKVLPKTYTAYYEPFIGGGAVLFELQPNKAVIND
CCCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEC
VNGELINLYNIIKYDVESLIEDLRKHENTSEYFYRIREIDRSKEEYEKLTNVEKASRIIY
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCHHHHCEEEE
LNKTCFNGLFRVNKAGEFNSPFGKYKNPNIVDEVTLRAVNKYFNKANIKILNGDFEQSLK
EEHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHH
RIRKGAFVYLDPPYDPVSSSANFTGYDKGGFNRAEQIRLKNLCDKLDKKGVKFLLSNSAT
HHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
DFIKELYKDYNIKVVKAKRTINSNGNSRGAVDEVLVRNYE
HHHHHHHHCCCEEEEEEEEEECCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MKKNKIILAAPVLKWVGGKRQLMGEIKKVLPKTYTAYYEPFIGGGAVLFELQPNKAVIND
CCCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEC
VNGELINLYNIIKYDVESLIEDLRKHENTSEYFYRIREIDRSKEEYEKLTNVEKASRIIY
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCHHHHCEEEE
LNKTCFNGLFRVNKAGEFNSPFGKYKNPNIVDEVTLRAVNKYFNKANIKILNGDFEQSLK
EEHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHH
RIRKGAFVYLDPPYDPVSSSANFTGYDKGGFNRAEQIRLKNLCDKLDKKGVKFLLSNSAT
HHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
DFIKELYKDYNIKVVKAKRTINSNGNSRGAVDEVLVRNYE
HHHHHHHHCCCEEEEEEEEEECCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7793939; 11467810 [H]